Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,199	T→A	R133R (CGT→CGA)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,199	0	T	A	98.6%	211.7 / NA	70	R133R (CGT→CGA)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (29/40);  minor base C (1/0);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCG‑ATGACCCTGGAAAAGAA‑TATGCC‑GATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCG  >  NC_000913/2912‑3467                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                              gTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGTTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCAGgcagc                                                                                                                                                                                                                                                                   >  2:136214/1‑300 (MQ=255) gTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGgccgc                                                                                                                                                                                                                                                                   >  2:136267/1‑297 (MQ=255) gTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGgcagc                                                                                                                                                                                                                                                                   >  1:454414/1‑300 (MQ=255)         ggcAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGTGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTa                                                                                                                                                                                                                                                           <  1:216236/300‑1 (MQ=255)             gagaCATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCATGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGAc                                                                                                                                                                                                                                                       <  2:395229/300‑1 (MQ=255)              agaCATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGAc                                                                                                                                                                                                                                                       <  2:410962/299‑1 (MQ=255)                 cATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAAc                                                                                                                                                                                                                                                    >  2:603531/1‑299 (MQ=255)                       ttctcAACAACCTCTTACGCTTTGCCGATAAGCTTCCTTCAGAACCACGGTAAAATATCGTTTATCAGTGCTGGGATCGTTTTTGCCAGGAGCTTGGCAGGCAAATGTCGGTGGCG‑ATGACCCTGGAAAAAAA‑TATGCC‑AATCGGTTCTGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTTCGGCAGCATTCATGACGACAACGTGGCAc                                                                                                                                                                                                                                             <  2:390753/299‑1 (MQ=255)                       gTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGGCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGGAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCAc                                                                                                                                                                                                                                             <  2:648081/300‑1 (MQ=255)                           ccacaacCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGNCACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgt                                                                                                                                                                                                                                          >  1:135923/3‑299 (MQ=255)                           caacaaCCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgt                                                                                                                                                                                                                                          >  2:184423/1‑299 (MQ=255)                           caacaaCCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgt                                                                                                                                                                                                                                          >  1:53487/1‑299 (MQ=255)                                  cTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTtctt                                                                                                                                                                                                                                  >  1:489019/1‑299 (MQ=255)                                      gACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTggtg                                                                                                                                                                                                                              <  1:348333/300‑1 (MQ=255)                                            ttGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGc                                                                                                                                                                                                                        <  1:913/300‑1 (MQ=255)                                              gCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCa                                                                                                                                                                                                                       >  1:235846/1‑299 (MQ=255)                                                 gATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGGGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtg                                                                                                                                                                                                                   <  1:426808/300‑1 (MQ=255)                                                 gATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtg                                                                                                                                                                                                                   <  1:426903/300‑1 (MQ=255)                                                 gATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATAAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGt                                                                                                                                                                                                                     <  1:695291/298‑1 (MQ=255)                                                           cGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCga                                                                                                                                                                                                           >  2:69869/1‑298 (MQ=255)                                                                        ggAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatcat                                                                                                                                                                                            <  1:436179/300‑1 (MQ=255)                                                                             atatCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATcagc                                                                                                                                                                                        >  2:488849/1‑299 (MQ=255)                                                                                          ttgctagaagcgcccttGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑CATCGGTTCGGGCTTCGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGACTGCACACTGTGGCAAGCCGCTTAATGACACTCGTTTGTTGGCTTTGATGGGCGAGCTGGAAGGACGCATCTCCGGCCGCACTCATTACGCCAACGTGGCACCGTGTTTTCTCGGTGGTATGCCGTTGATGATCGAAGAAAACGACATCATCGGCCAGCAAGTGCCa                                                                                                                                                                            >  2:679497/16‑298 (MQ=255)                                                                                          gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATTGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCAAGCAAGTGc                                                                                                                                                                              >  2:468591/1‑296 (MQ=255)                                                                                          gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGCCAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                                                                                                                                                                              >  2:198176/1‑296 (MQ=255)                                                                                          gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGCTCGAAGAAAACGACATCATCAGCCAGCAAGTGCCa                                                                                                                                                                            >  2:29265/1‑298 (MQ=255)                                                                                          gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCa                                                                                                                                                                            >  1:126819/1‑298 (MQ=255)                                                                                          gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCATGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAAAGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGCTCGAAGAGAACGACATCATCATCCGGCAAGTGCCa                                                                                                                                                                            >  2:30784/1‑298 (MQ=255)                                                                                           tacTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAggg                                                                                                                                                                         <  1:519027/298‑1 (MQ=255)                                                                                           tGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAggg                                                                                                                                                                         <  1:518965/300‑1 (MQ=255)                                                                                                          tGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCtg                                                                                                                                                         <  1:571289/300‑1 (MQ=255)                                                                                                                gagcttggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGt                                                                                                                                                    >  2:438674/10‑299 (MQ=255)                                                                                                                  gcttggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCt                                                                                                                                                 <  2:558439/293‑1 (MQ=255)                                                                                                                      ggcaaGCAAATTCCAGTTGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTTGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCTGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCg                                                                                                                                             <  2:489019/297‑1 (MQ=255)                                                                                                                      ggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGNTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCg                                                                                                                                             <  1:149383/297‑1 (MQ=255)                                                                                                                      ggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCg                                                                                                                                             <  1:599649/297‑1 (MQ=255)                                                                                                                         agacgaaTTCCAGTGGCNCATGACTCTGGAAAAGAANTATGCCAGATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTNGGCTGTGGGTGCTGGc                                                                                                                                              <  1:581687/295‑1 (MQ=255)                                                                                                                                       ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGt                                                                                                                            <  1:120717/300‑1 (MQ=255)                                                                                                                                       ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTCAAGt                                                                                                                            >  2:614327/1‑300 (MQ=255)                                                                                                                                       ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGACAGCAAGTGCAAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGt                                                                                                                            >  2:458130/1‑300 (MQ=255)                                                                                                                                       ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGAGGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTTGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGt                                                                                                                            <  1:119723/300‑1 (MQ=255)                                                                                                                                        gCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGt                                                                                                                            <  1:119739/299‑1 (MQ=255)                                                                                                                                                ctcTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGAc                                                                                                                      >  1:189942/3‑298 (MQ=255)                                                                                                                                                        aaGAG‑TATGCC‑AATCGGTTCGGGCGTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGAGGGCGATGAATGAACACTTCGGCAAGCCGCTTAATGACACTCGTTGGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACTTGGCACCGTGTTTTCTTGTGGGTATGCAGGGGATGATCGAAGAAAACGACACCAGCAGCCAGCAAGGGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGGATCCTGGGATTAAAGTCTCGACGGCAGAAGcc                                                                                                            <  1:331504/300‑1 (MQ=255)                                                                                                                                                        aaGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGTCTCGACGGCAGAAGcc                                                                                                            <  1:391586/300‑1 (MQ=255)                                                                                                                                                        aaGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGcc                                                                                                            <  1:392749/300‑1 (MQ=255)                                                                                                                                                        aaGAA‑TATACC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGcc                                                                                                            <  1:108031/300‑1 (MQ=255)                                                                                                                                                         aGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTTGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCGGGAAGCACGAATCTCCGGCAGTATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGcc                                                                                                            <  2:561126/299‑1 (MQ=255)                                                                                                                                                              tatGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCAAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGTCTCGACGGCAGAAGCCAGGGc                                                                                                       <  1:87981/300‑1 (MQ=255)                                                                                                                                                                 tgaaaagtggggtgggtttAGGCTCCAGCGCCGGTGAGGGGGTCGTGGCGCTGATGGCGATGAATAAACACGGTTGTAAGCCGCTTAATGACACTCGTTTACTAGCTGTGATTGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCATCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCATGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGc                                                                                                       <  2:111252/281‑1 (MQ=255)                                                                                                                                                                                  ttAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGCCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTAt                                                                                    <  1:290561/300‑1 (MQ=255)                                                                                                                                                                                   tagaatccagcgcttggtCGGTGGTCGAGGTGCTGATTGCGATGAGTAAACACTGTGACAAGCCGCTTAATGGCATTCGTTTGCTGGCTTTGATGGACGAGCGGGAAGGACGAATCTCTTGCGGCATGCATTACGACAACGTGGCACCGTTTTTTCTTGGTGGCATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTAGCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTAt                                                                                    <  2:52251/282‑1 (MQ=255)                                                                                                                                                                                     ggCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTAGAAGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATc                                                                                   >  2:311813/1‑298 (MQ=255)                                                                                                                                                                                                  gTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTAGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgca                                                                    >  2:110224/1‑300 (MQ=255)                                                                                                                                                                                                  gTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCAGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTTTGGGTGGTATGCAGTTGATGGTCGAAGAGAACGACATAATCGGCAAGAAAGTGCAAGGGTTTGATGAGT‑GGCTGCGGGTGCTGGCGTATCGGGGGATTAAAGTCTAGCCGGCAGAAGCCAGGGCTGTTTTACCGGCGAAATATCGCCGCTAGGcttgc                                                                     >  2:110232/1‑299 (MQ=255)                                                                                                                                                                                                   ttCGCTGGTCGCTGCTCTGATGGCTATGAATGAACACTTTGACAATCCGCTTAATGACACTCGTTTGATTGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGGGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgcat                                                                   <  2:399667/300‑1 (MQ=255)                                                                                                                                                                                                    tCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgcat                                                                   <  1:110172/299‑1 (MQ=255)                                                                                                                                                                                                                    tGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATc                                                  <  1:403898/300‑1 (MQ=255)                                                                                                                                                                                                                             tgaatgaaCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGANCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTggctggct                                         >  2:493491/1‑300 (MQ=255)                                                                                                                                                                                                                              gaatgaaCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCAAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTAGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGACAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCtt                                        >  2:436047/1‑300 (MQ=255)                                                                                                                                                                                                                                  gaaCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTGTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCttcatt                                    <  1:549857/300‑1 (MQ=255)                                                                                                                                                                                                                                     cacTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCAc                                 >  2:680041/1‑300 (MQ=255)                                                                                                                                                                                                                                       ctgtggCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAGACGACATCATCAGCAAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGc                               >  2:594860/5‑300 (MQ=255)                                                                                                                                                                                                                                                 ccGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGCTGATGATCGAAGAAAACGACATCATCAGCAAGCAAGTGCCGGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCAAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTc                      >  2:380904/1‑299 (MQ=255)                                                                                                                                                                                                                                                   gCTTAATGACACTCGNTTGCTGGCTTTGATGGGCNAGCTGGAAGTACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCANTCACGCCTGCTATTcc                     <  1:591952/298‑1 (MQ=255)                                                                                                                                                                                                                                                               tcttttGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTNCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCt       <  1:136214/297‑1 (MQ=255)                                                                                                                                                                                                                                                               tCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTNCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCt       <  1:136267/300‑1 (MQ=255)                                                                                                                                                                                                                                                                cGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTNTCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGTGCAGTATCGCCGCCAGGATTGCCTTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCtt      >  2:220398/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  ttggCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGc        <  2:696663/293‑1 (MQ=255)                                                                                                                                                                                                                                                                    tGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCcg  <  1:603531/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                              GTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCG‑ATGACCCTGGAAAAGAA‑TATGCC‑GATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCG  >  NC_000913/2912‑3467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑