Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,142	C→T	C114C (TGC→TGT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,142	0	C	T	98.8%	265.5 / NA	81	C114C (TGC→TGT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (40/40);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCG‑ATGACCCTGGAAAAGAA‑TATGCC‑GATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCAC  >  NC_000913/2847‑3436                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                        ggTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCa                                                                                                                                                                                                                                                                                                     >  2:630940/1‑300 (MQ=255)  gTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGTAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATAGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCaa                                                                                                                                                                                                                                                                                                    <  1:258703/300‑1 (MQ=255)           gCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTaa                                                                                                                                                                                                                                                                                           >  1:460798/1‑300 (MQ=255)           gCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTAGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTaa                                                                                                                                                                                                                                                                                           >  2:87981/1‑300 (MQ=255)             tCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGTAGATGTAGTCACGGTTGAGGCGGCATAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATg                                                                                                                                                                                                                                                                                         <  1:94666/300‑1 (MQ=255)             tCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATg                                                                                                                                                                                                                                                                                         <  1:94161/300‑1 (MQ=255)               ggggCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGNGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGa                                                                                                                                                                                                                                                                                        <  2:508181/299‑1 (MQ=255)                gggCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGaca                                                                                                                                                                                                                                                                                      >  2:591952/1‑300 (MQ=255)                    ggcgtTGACACCTGTTGATGGTGCATTGCTCGTAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGTGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGacac                                                                                                                                                                                                                                                                                     <  2:384329/297‑1 (MQ=255)                                 gTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCttt                                                                                                                                                                                                                                                                      >  1:696663/1‑299 (MQ=255)                                 gTTGATGGTGCATTGCTAGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCA‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCttt                                                                                                                                                                                                                                                                      >  2:627299/1‑299 (MQ=255)                                     aTGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCATGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGAt                                                                                                                                                                                                                                                                   >  1:633622/1‑298 (MQ=255)                                        gTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGc                                                                                                                                                                                                                                                               >  1:395229/1‑299 (MQ=255)                                               gCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGa                                                                                                                                                                                                                                                       >  2:403898/1‑300 (MQ=255)                                               gCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGa                                                                                                                                                                                                                                                       >  1:175722/1‑300 (MQ=255)                                                   gtagaTGTAGTCACGGTTGAGGCGGCATAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACTGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAgga                                                                                                                                                                                                                                                   <  2:398959/298‑2 (MQ=255)                                                    tagaTGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAggac                                                                                                                                                                                                                                                  >  1:561126/2‑298 (MQ=255)                                                    gagaTGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAggac                                                                                                                                                                                                                                                  >  1:105834/1‑298 (MQ=255)                                                      gaTGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAggacga                                                                                                                                                                                                                                                >  1:452935/1‑296 (MQ=255)                                                                  gTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGTTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCAGgcagc                                                                                                                                                                                                                                    >  2:136214/1‑300 (MQ=255)                                                                  gTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGgccgc                                                                                                                                                                                                                                    >  2:136267/1‑297 (MQ=255)                                                                  gTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGgcagc                                                                                                                                                                                                                                    >  1:454414/1‑300 (MQ=255)                                                                          ggcAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGTGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTa                                                                                                                                                                                                                            <  1:216236/300‑1 (MQ=255)                                                                              gagaCATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCATGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGAc                                                                                                                                                                                                                        <  2:395229/300‑1 (MQ=255)                                                                               agaCATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGAc                                                                                                                                                                                                                        <  2:410962/299‑1 (MQ=255)                                                                                  cATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAAc                                                                                                                                                                                                                     >  2:603531/1‑299 (MQ=255)                                                                                        ttctcAACAACCTCTTACGCTTTGCCGATAAGCTTCCTTCAGAACCACGGTAAAATATCGTTTATCAGTGCTGGGATCGTTTTTGCCAGGAGCTTGGCAGGCAAATGTCGGTGGCG‑ATGACCCTGGAAAAAAA‑TATGCC‑AATCGGTTCTGGCTTAGGCTCCAGCGCCTGTGCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTTCGGCAGCATTCATGACGACAACGTGGCAc                                                                                                                                                                                                              <  2:390753/299‑1 (MQ=255)                                                                                        gTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGGCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGGAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCAc                                                                                                                                                                                                              <  2:648081/300‑1 (MQ=255)                                                                                            ccacaacCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGNCACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgt                                                                                                                                                                                                           >  1:135923/3‑299 (MQ=255)                                                                                            caacaaCCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgt                                                                                                                                                                                                           >  2:184423/1‑299 (MQ=255)                                                                                            caacaaCCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCgt                                                                                                                                                                                                           >  1:53487/1‑299 (MQ=255)                                                                                                   cTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTtctt                                                                                                                                                                                                   >  1:489019/1‑299 (MQ=255)                                                                                                       gACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTggtg                                                                                                                                                                                               <  1:348333/300‑1 (MQ=255)                                                                                                             ttGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGc                                                                                                                                                                                         <  1:913/300‑1 (MQ=255)                                                                                                               gCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCa                                                                                                                                                                                        >  1:235846/1‑299 (MQ=255)                                                                                                                  gATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGGGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtg                                                                                                                                                                                    <  1:426808/300‑1 (MQ=255)                                                                                                                  gATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTtg                                                                                                                                                                                    <  1:426903/300‑1 (MQ=255)                                                                                                                  gATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATAAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGt                                                                                                                                                                                      <  1:695291/298‑1 (MQ=255)                                                                                                                            cGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCga                                                                                                                                                                            >  2:69869/1‑298 (MQ=255)                                                                                                                                         ggAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGAcatcat                                                                                                                                                             <  1:436179/300‑1 (MQ=255)                                                                                                                                              atatCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATcagc                                                                                                                                                         >  2:488849/1‑299 (MQ=255)                                                                                                                                                           ttgctagaagcgcccttGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑CATCGGTTCGGGCTTCGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGACTGCACACTGTGGCAAGCCGCTTAATGACACTCGTTTGTTGGCTTTGATGGGCGAGCTGGAAGGACGCATCTCCGGCCGCACTCATTACGCCAACGTGGCACCGTGTTTTCTCGGTGGTATGCCGTTGATGATCGAAGAAAACGACATCATCGGCCAGCAAGTGCCa                                                                                                                                             >  2:679497/16‑298 (MQ=255)                                                                                                                                                           gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATTGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCAAGCAAGTGc                                                                                                                                               >  2:468591/1‑296 (MQ=255)                                                                                                                                                           gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGCCAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGc                                                                                                                                               >  2:198176/1‑296 (MQ=255)                                                                                                                                                           gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGCTCGAAGAAAACGACATCATCAGCCAGCAAGTGCCa                                                                                                                                             >  2:29265/1‑298 (MQ=255)                                                                                                                                                           gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCa                                                                                                                                             >  1:126819/1‑298 (MQ=255)                                                                                                                                                           gTGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCATGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAAAGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGCTCGAAGAGAACGACATCATCATCCGGCAAGTGCCa                                                                                                                                             >  2:30784/1‑298 (MQ=255)                                                                                                                                                            tacTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAggg                                                                                                                                          <  1:519027/298‑1 (MQ=255)                                                                                                                                                            tGCTGGGAGCGTTTTTGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAggg                                                                                                                                          <  1:518965/300‑1 (MQ=255)                                                                                                                                                                           tGCCAGGAGCTTGGCAAGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCtg                                                                                                                          <  1:571289/300‑1 (MQ=255)                                                                                                                                                                                 gagcttggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGt                                                                                                                     >  2:438674/10‑299 (MQ=255)                                                                                                                                                                                   gcttggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AAGCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCt                                                                                                                  <  2:558439/293‑1 (MQ=255)                                                                                                                                                                                       ggcaaGCAAATTCCAGTTGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTTGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCTGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCg                                                                                                              <  2:489019/297‑1 (MQ=255)                                                                                                                                                                                       ggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGNTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCg                                                                                                              <  1:149383/297‑1 (MQ=255)                                                                                                                                                                                       ggcaaGCAAATTCCAGTGGCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCg                                                                                                              <  1:599649/297‑1 (MQ=255)                                                                                                                                                                                          agacgaaTTCCAGTGGCNCATGACTCTGGAAAAGAANTATGCCAGATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGTNGGCTGTGGGTGCTGGc                                                                                                               <  1:581687/295‑1 (MQ=255)                                                                                                                                                                                                        ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGt                                                                                             <  1:120717/300‑1 (MQ=255)                                                                                                                                                                                                        ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTCAAGt                                                                                             >  2:614327/1‑300 (MQ=255)                                                                                                                                                                                                        ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGACAGCAAGTGCAAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGt                                                                                             >  2:458130/1‑300 (MQ=255)                                                                                                                                                                                                        ggCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGAGGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTTGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGt                                                                                             <  1:119723/300‑1 (MQ=255)                                                                                                                                                                                                         gCG‑ATGACTCTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGt                                                                                             <  1:119739/299‑1 (MQ=255)                                                                                                                                                                                                                 ctcTGGAAAAGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGAc                                                                                       >  1:189942/3‑298 (MQ=255)                                                                                                                                                                                                                         aaGAG‑TATGCC‑AATCGGTTCGGGCGTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGAGGGCGATGAATGAACACTTCGGCAAGCCGCTTAATGACACTCGTTGGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACTTGGCACCGTGTTTTCTTGTGGGTATGCAGGGGATGATCGAAGAAAACGACACCAGCAGCCAGCAAGGGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGGATCCTGGGATTAAAGTCTCGACGGCAGAAGcc                                                                             <  1:331504/300‑1 (MQ=255)                                                                                                                                                                                                                         aaGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGTCTCGACGGCAGAAGcc                                                                             <  1:391586/300‑1 (MQ=255)                                                                                                                                                                                                                         aaGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGcc                                                                             <  1:392749/300‑1 (MQ=255)                                                                                                                                                                                                                         aaGAA‑TATACC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGcc                                                                             <  1:108031/300‑1 (MQ=255)                                                                                                                                                                                                                          aGAA‑TATGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTTGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCGGGAAGCACGAATCTCCGGCAGTATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGcc                                                                             <  2:561126/299‑1 (MQ=255)                                                                                                                                                                                                                               tatGCC‑AATCGGTTCGGGCTTAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCAAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGTCTCGACGGCAGAAGCCAGGGc                                                                        <  1:87981/300‑1 (MQ=255)                                                                                                                                                                                                                                  tgaaaagtggggtgggtttAGGCTCCAGCGCCGGTGAGGGGGTCGTGGCGCTGATGGCGATGAATAAACACGGTTGTAAGCCGCTTAATGACACTCGTTTACTAGCTGTGATTGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCATCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCATGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGc                                                                        <  2:111252/281‑1 (MQ=255)                                                                                                                                                                                                                                                   ttAGGCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGCCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTAt                                                     <  1:290561/300‑1 (MQ=255)                                                                                                                                                                                                                                                    tagaatccagcgcttggtCGGTGGTCGAGGTGCTGATTGCGATGAGTAAACACTGTGACAAGCCGCTTAATGGCATTCGTTTGCTGGCTTTGATGGACGAGCGGGAAGGACGAATCTCTTGCGGCATGCATTACGACAACGTGGCACCGTTTTTTCTTGGTGGCATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTAGCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTAt                                                     <  2:52251/282‑1 (MQ=255)                                                                                                                                                                                                                                                      ggCTCCAGCGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTAGAAGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATc                                                    >  2:311813/1‑298 (MQ=255)                                                                                                                                                                                                                                                                   gTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTAGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgca                                     >  2:110224/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   gTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCAGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTTTGGGTGGTATGCAGTTGATGGTCGAAGAGAACGACATAATCGGCAAGAAAGTGCAAGGGTTTGATGAGT‑GGCTGCGGGTGCTGGCGTATCGGGGGATTAAAGTCTAGCCGGCAGAAGCCAGGGCTGTTTTACCGGCGAAATATCGCCGCTAGGcttgc                                      >  2:110232/1‑299 (MQ=255)                                                                                                                                                                                                                                                                    ttCGCTGGTCGCTGCTCTGATGGCTATGAATGAACACTTTGACAATCCGCTTAATGACACTCGTTTGATTGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGGGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGTGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgcat                                    <  2:399667/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     tCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGattgcat                                    <  1:110172/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tGATGGCGATGAATGAACACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATc                   <  1:403898/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tgaatgaaCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGANCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTggctggct          >  2:493491/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               gaatgaaCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCAAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTAGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGACAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCtt         >  2:436047/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   gaaCACTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTGTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCttcatt     <  1:549857/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      cacTGTGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGACGAATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTTGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCAc  >  2:680041/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                        GGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATTCCAGTGGCG‑ATGACCCTGGAAAAGAA‑TATGCC‑GATCGGTTCGGGCTTAGGCTCCAGTGCCTGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGACACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTACGACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATCATCAGCCAGCAAGTGCCAGGGTTTGATGAGT‑GGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCAC  >  NC_000913/2847‑3436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑