Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,348,366	C→A	T328K (ACG→AAG)	nrdB →	ribonucleoside‑diphosphate reductase 1 subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,348,366	0	C	A	100.0%	184.9 / NA	55	T328K (ACG→AAG)	nrdB	ribonucleoside‑diphosphate reductase 1 subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/31);  total (24/31)

TTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGACGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCC  >  NC_000913/2348078‑2348658                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                     ttCGCCTGATTGCCCTCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTTAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGTAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAAcc                                                                                                                                                                                                                                                                                           <  2:234890/300‑1 (MQ=255) ttCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAAcc                                                                                                                                                                                                                                                                                           <  2:234916/300‑1 (MQ=255)  tCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAAccc                                                                                                                                                                                                                                                                                          <  2:628660/300‑1 (MQ=255)       tGATTGCCCGCGACGAANCCCTGCACCTGACCGGCANCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTTAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAANCGCTCCAACCCGATcc                                                                                                                                                                                                                                                                                     <  1:590464/300‑1 (MQ=255)       tGATTGCCCGCGACGAANCCCTGCACCTGACCGGCANCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAANCGCTCCAACCCGATcc                                                                                                                                                                                                                                                                                     <  1:590441/300‑1 (MQ=255)                     gaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGANTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAacac                                                                                                                                                                                                                                                                        <  2:550095/299‑1 (MQ=255)                      aaGCCCTGCACCTTACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGTCTAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGTGCGGATTATCTGTTCCGCTACGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGCGGATCAacac                                                                                                                                                                                                                                                                        <  2:204784/298‑1 (MQ=255)                      aaGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATGGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAacac                                                                                                                                                                                                                                                                        <  2:59446/298‑1 (MQ=255)                                ccTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTc                                                                                                                                                                                                                                                            >  1:374896/1‑300 (MQ=255)                                ccTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCAACAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCAGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTc                                                                                                                                                                                                                                                            >  1:374018/1‑300 (MQ=255)                                    aCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGAt                                                                                                                                                                                                                                                        <  1:43750/300‑1 (MQ=255)                                       ggCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATaa                                                                                                                                                                                                                                                      <  2:561050/299‑1 (MQ=255)                                        gCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACg                                                                                                                                                                                                                                                    <  1:412539/300‑1 (MQ=255)                                               gCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAgat                                                                                                                                                                                                                                             >  1:534881/1‑298 (MQ=255)                                                        gAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAAGTGCAGGTTGCTCCGCa                                                                                                                                                                                                                                    >  2:154747/1‑300 (MQ=255)                                                            ctgctgCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTTAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCAACAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTACAGAAGCGCTCAAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATaa                                                                                                                                                                                                                                                      >  2:67303/1‑278 (MQ=255)                                                            ctgctgCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATTCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAAACCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAAGTTGCTCCGCAggaa                                                                                                                                                                                                                                >  2:68158/1‑300 (MQ=255)                                                             tgctgcGCAGCGGCGCGGACGATCCTTAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAggaag                                                                                                                                                                                                                               <  1:519063/300‑1 (MQ=255)                                                             tgctgcGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAggaag                                                                                                                                                                                                                               <  1:518523/300‑1 (MQ=255)                                                             tgctgcGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTGCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTGTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAggaag                                                                                                                                                                                                                               <  2:410715/300‑1 (MQ=255)                                                                     aGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTc                                                                                                                                                                                                                       <  1:408075/300‑1 (MQ=255)                                                                           gcgGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCANCAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTAGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTc                                                                                                                                                                                                                  <  1:513715/299‑1 (MQ=255)                                                                               acaaTCCTGAGACGGCGGAAATTGACTAAGGGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCATGCAGCTCAACAGGAGAAAGACTGGGCGGACTATCTGTTCCGCGACGGTTCGATGATTGGGCTGAATAANGACATTCTCTGCCAGTACGTTGAATACATCACCAAGATCCGTATGCAGGCAGTCGGTTTGGAGCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATc                                                                                                                                                                                                             <  2:230471/297‑1 (MQ=255)                                                                               aCGATTCTGAGATTGCGGAAATTGCCGAAAAGTGGAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGTGCAACAGTAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAANGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCTTATGCAGGCAGTCGGTTTGGATCTGCCGTTCTAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATc                                                                                                                                                                                                             <  2:230625/300‑1 (MQ=255)                                                                               aCGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAANGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCATGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATc                                                                                                                                                                                                             <  2:230683/300‑1 (MQ=255)                                                                               aCGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATc                                                                                                                                                                                                             <  1:208749/300‑1 (MQ=255)                                                                                  aTCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTACCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCt                                                                                                                                                                                                            >  1:659148/1‑298 (MQ=255)                                                                                             gCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGAtt                                                                                                                                                                                               <  1:172101/300‑1 (MQ=255)                                                                                                    ttGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTc                                                                                                                                                                                          >  1:168399/1‑298 (MQ=255)                                                                                                             agTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAAAAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCAAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTCTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCACGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATATGGTCGGGCAGATTGACTCGGAAGTGGac                                                                                                                                                                                >  2:350552/1‑299 (MQ=255)                                                                                                              gtgtAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACNAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGacac                                                                                                                                                                              >  2:499216/1‑300 (MQ=255)                                                                                                                  aaGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACcgac                                                                                                                                                                          >  1:649615/1‑300 (MQ=255)                                                                                                                            gCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTa                                                                                                                                                                >  2:277279/1‑300 (MQ=255)                                                                                                                                            tCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATg                                                                                                                                                <  1:277279/300‑1 (MQ=255)                                                                                                                                                          aggaggAAGATTGGGCGGATTATCTGTTCCGCGACGGTTTGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTAccc                                                                                                                                   <  1:545120/299‑1 (MQ=255)                                                                                                                                                          aGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTAccc                                                                                                                                   <  1:5146/299‑1 (MQ=255)                                                                                                                                                           ggAGAAAGACTGAGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGTTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCt                                                                                                                                  >  1:259283/1‑299 (MQ=255)                                                                                                                                                                           gATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGcacac                                                                                                                 <  1:411392/300‑1 (MQ=255)                                                                                                                                                                           gATTATCTGTTCCGCGACAGTTCGATGATTGGTATGAATAAAGACATTCTCTGCCAGTACGGTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTGTGGATCTGCCTTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGGGGAAGTCAGTTCTTATCCGGTCGGGCAGATTGACTCGTAAGTGGACACCGACGAGTTGAGTAACTTCCAGCTCTGATGGcc                                                                                                                                             <  2:286928/272‑1 (MQ=255)                                                                                                                                                                                   gTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGNAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAActgct                                                                                                          >  1:493666/1‑299 (MQ=255)                                                                                                                                                                                        gcgACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGc                                                                                                      <  1:208462/298‑1 (MQ=255)                                                                                                                                                                                              gTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGTCAGGATGa                                                                                              >  2:307047/1‑300 (MQ=255)                                                                                                                                                                                                         ggTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGTAGTCGGTTTGGATCTGCCGTTCCAGAAGTGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACAGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGTACACAACTGCTGTGCCAGGATGACCAcccttcac                                                                                   >  2:81805/1‑298 (MQ=255)                                                                                                                                                                                                                                     aCGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAAtgt                                                       >  1:112951/1‑300 (MQ=255)                                                                                                                                                                                                                                       gTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAAtgt                                                       >  1:391489/1‑298 (MQ=255)                                                                                                                                                                                                                                       gTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCAATGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTATGGCGGCGCTGGAATCCCACAAtgt                                                       >  2:259493/1‑298 (MQ=255)                                                                                                                                                                                                                                       gTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGAAGATTTGAGTAACTTCCAGCTCTGCTGGCACGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCACTTCTGGCGGCGCTGGAATCCAACAAtgt                                                       >  2:278720/1‑298 (MQ=255)                                                                                                                                                                                                                                       gTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGAAGATTTGAGTAACTTACAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTACCTTATGGCGGCGCTGGAATCCCACAAtgt                                                       >  2:278669/1‑298 (MQ=255)                                                                                                                                                                                                                                                   aCCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTAcc                                         <  1:265026/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aCCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTAc                                          <  1:565725/299‑1 (MQ=255)                                                                                                                                                                                                                                                      aaTATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGGTCTTATCTCGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAg                                       <  2:365850/299‑1 (MQ=255)                                                                                                                                                                                                                                                          tCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCATGCGCATCACTGGCACACAAATGCTGTGCCAGGATGAACACCCTTACCTTATGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTc                                   >  2:35068/1‑299 (MQ=255)                                                                                                                                                                                                                                                                   aggcagTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGTTGTCTGATAACGTGCAGGTTGCTCCGCAGGAATTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGTAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAgg                            <  2:119421/297‑1 (MQ=255)                                                                                                                                                                                                                                                                          cGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGc                  >  2:620859/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          ttCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCTCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGcc  >  1:603447/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                     TTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGACGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACACGCC  >  NC_000913/2348078‑2348658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑