Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,171,417	G→A	L850L (CTG→TTG)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,171,417	0	G	A	100.0%	180.4 / NA	54	L850L (CTG→TTG)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/33);  total (21/33)

GCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATTGCTTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCATTGCCATATTCAGCGTACGTGGGATCGGCGTTGCA  >  NC_000913/1171132‑1171700                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                            gcgcAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATTGCTTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCa                                                                                                                                                                                                                                                                               >  2:425806/1‑300 (MQ=255)        aCCTGCCCCGAGATCTTCCAGCGAGGCAATTGCTTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCTGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGccc                                                                                                                                                                                                                                                                        <  2:387373/300‑1 (MQ=255)                 gagaTCTTCCAGCGAGGCAATTGCTTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGAc                                                                                                                                                                                                                                                                   <  1:458654/296‑1 (MQ=255)                 gagaTCTTCCAGCGAGGCAATTGCTTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGAt                                                                                                                                                                                                                                                               >  2:451285/1‑300 (MQ=255)                  agaTCTTCCAGCGAGGCAATTTCTTCAAGACGTTTTTGAGCATCGTTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGAc                                                                                                                                                                                                                                                                   <  1:458662/295‑1 (MQ=255)                                   aaTTTCTTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCACTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTg                                                                                                                                                                                                                                              <  2:32847/299‑1 (MQ=255)                                            aaGACGTTTTTGTGCATCGTTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACTTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTc                                                                                                                                                                                                                                    <  2:344547/300‑1 (MQ=255)                                            aaGACGTTTTTGTGCATCGTTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTc                                                                                                                                                                                                                                    <  2:476133/300‑1 (MQ=255)                                             aGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTc                                                                                                                                                                                                                                    <  2:6093/299‑1 (MQ=255)                                                                tAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCggcggc                                                                                                                                                                                                                  <  2:244486/298‑1 (MQ=255)                                                                                     gtgtCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCAcatcat                                                                                                                                                                                           >  2:256974/1‑300 (MQ=255)                                                                                     gtgtCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAANAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTNGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCAcatcat                                                                                                                                                                                           >  2:525894/1‑300 (MQ=255)                                                                                     gtgtCAGCAACCATGCATATGCCTGATAATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACCAACCAGCACGTTGAAACGCTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCAAGCCGTTCGGCGGCTTTCTGAATGTTTTCCAcatcat                                                                                                                                                                                           >  2:128410/1‑300 (MQ=255)                                                                                       gtCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGCAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCAcatcat                                                                                                                                                                                           >  2:26115/1‑298 (MQ=255)                                                                                       gtCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCAtt                                                                                                                                                                                          >  1:390310/1‑299 (MQ=255)                                                                                       gtCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCAtt                                                                                                                                                                                          >  2:24993/1‑299 (MQ=255)                                                                                       gtCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTACACATCAtt                                                                                                                                                                                          >  1:390285/1‑299 (MQ=255)                                                                                          aGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGCGACTGGTGC‑AGCTGCGCC‑AGACCGAATTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACAGCATTGTag                                                                                                                                                                                      <  2:600440/300‑1 (MQ=255)                                                                                          aGCAACCATGCATATGCCTGATGAGGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTag                                                                                                                                                                                      <  2:600448/300‑1 (MQ=255)                                                                                             aaCCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGATTGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTGCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTagag                                                                                                                                                                                    <  2:182521/299‑1 (MQ=255)                                                                                             aaCCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCNGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTagag                                                                                                                                                                                    <  1:140455/299‑1 (MQ=255)                                                                                             aaCCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTagag                                                                                                                                                                                    <  1:259159/299‑1 (MQ=255)                                                                                              aCCATGCATATGCCTGATGATGCGAC‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCACGTGACCGATGGCGATCCGCGCTTCTGGCACAAGTTCTGCCGGCAGTTCGGCGGCTTTCTGAATGTTTTCCAAATCATTGTAGAGat                                                                                                                                                                                  >  2:662173/1‑300 (MQ=255)                                                                                              aCCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAACCCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGat                                                                                                                                                                                  >  1:353847/1‑300 (MQ=255)                                                                                                                 atgCGAAACGTCCGACGNCGACCGCGTAACTGGTGCNAGCTGCGCCCAGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGNTAATAAACCTGtcctc                                                                                                                                                                   <  1:575577/300‑1 (MQ=255)                                                                                                                  tgCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCgcgc                                                                                                                                                              <  1:83076/300‑1 (MQ=255)                                                                                                                                            aaCTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGTTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGcc                                                                                                                                         <  2:319093/297‑1 (MQ=255)                                                                                                                                                ggTGC‑AGCGGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTATAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCgta                                                                                                                                  <  2:567025/300‑3 (MQ=255)                                                                                                                                                 gTGC‑AGCTGCGCC‑AGACCGAAGCGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGa                                                                                                                                  <  2:640027/299‑1 (MQ=255)                                                                                                                                                                     gAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGTTGTCTGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCGGCCCGTGACCGATGGCGATCCGCGCTCCTTGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCACGCTGTCATACTc                                                                                                               <  2:273811/300‑1 (MQ=255)                                                                                                                                                                     gAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTc                                                                                                               <  1:392785/300‑1 (MQ=255)                                                                                                                                                                      aaGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTATAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATAc                                                                                                                 <  2:638845/297‑1 (MQ=255)                                                                                                                                                                               gcgcgTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGGTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGAc                                                                                                         <  2:647266/296‑1 (MQ=255)                                                                                                                                                                                       aaTGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGTTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTATAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAAc                                                                                             <  2:52706/300‑1 (MQ=255)                                                                                                                                                                                                 gtgtTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTACCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAgacga                                                                                   >  2:691951/1‑300 (MQ=255)                                                                                                                                                                                                     tGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGAcggg                                                                               <  1:121105/300‑1 (MQ=255)                                                                                                                                                                                                                           ggTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGa                                                         >  1:363858/1‑300 (MQ=255)                                                                                                                                                                                                                             tttCGATAATGTTTGTACAAACCAGCACGTTGAAACGTTGATGATGTAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGAc                                                        <  2:375649/299‑1 (MQ=255)                                                                                                                                                                                                                                      tGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCNTGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCa                                              >  1:512304/1‑300 (MQ=255)                                                                                                                                                                                                                                      tGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCa                                              <  1:198434/300‑1 (MQ=255)                                                                                                                                                                                                                                      tGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTACCGGACCACCNTGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCa                                              >  1:512295/1‑300 (MQ=255)                                                                                                                                                                                                                                       ggTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGTGGCGTGGCGATAATCGACAGGTCACGCAt                                             <  1:399604/300‑1 (MQ=255)                                                                                                                                                                                                                                       ggTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCAt                                             <  1:399595/300‑1 (MQ=255)                                                                                                                                                                                                                                           gTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTcc                                          >  2:399138/1‑299 (MQ=255)                                                                                                                                                                                                                                           gTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCAACCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTcc                                          >  2:365203/1‑299 (MQ=255)                                                                                                                                                                                                                                                aaCCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCAt                                    >  1:89235/1‑300 (MQ=255)                                                                                                                                                                                                                                                        cGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCATTGCCatat                            >  2:440213/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gTTGAAGCGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCTGCGGCTTTCTGAATGTTTTCCACATCATTGTATAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGTACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCATTGCCatat                            <  2:597259/299‑1 (MQ=255)                                                                                                                                                                                                                                                          ttGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCATTGCCATATTc                          <  1:520813/300‑1 (MQ=255)                                                                                                                                                                                                                                                          ttGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCCCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCATTGCCATATTc                          <  2:482325/300‑1 (MQ=255)                                                                                                                                                                                                                                                           tGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGTCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCATTGCCATATTc                          <  2:57922/299‑1 (MQ=255)                                                                                                                                                                                                                                                               aCGTTGATGATGGAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTATAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCATTGCCATATTCAGCGt                     <  2:667581/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          ggAAATCATTCATCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCATTGCCATATTCAGCGTACGTGGGAt            >  2:51971/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                  ttcatCACCCGTTCCAATTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCAGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCATTGACATATTCAGCGTACGTGGGATCGGCGTTGCa  >  2:376053/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                            GCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATTGCTTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAA‑CGTCCGACG‑CGACCGCGTAACTGGTGC‑AGCTGCGCC‑AGACCGAAGTGATCCGCGCGTTCAATGATAATAGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTTGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGCTTCTGGCACCAGTTCTGCCAGCCGTTCGGCGGCTTTCTGAATGTTTTCCACATCATTGTAGAGATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGCGTGGCGATAATCGACAGGTCACGCATTCCGCTCATTGCCATATTCAGCGTACGTGGGATCGGCGTTGCA  >  NC_000913/1171132‑1171700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑