| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,117,707 | G→A | G301S (GGT→AGT) | trhO → | tRNA U34 hydroxylase TrhO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,117,707 | 0 | G | A | 98.5% | 209.4 / ‑4.4 | 66 | G301S (GGT→AGT) | trhO | tRNA U34 hydroxylase TrhO |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (33/32); total (33/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GTGAAAAGGCCAGTGCCTGGATGAAACATAACGGATTCAACAAAGTCTGGCATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AGGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTT > NC_000913/1117423‑1117998 | gtgAAAAGGCCAGTGCCTGGATGAAACATAACGGATTCAACAAAGTCTGGCATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTgaga < 1:409715/300‑1 (MQ=255) ggCCAGTGCCTGGATGAAACATAACGGATTCAACAAAGTCTGGCATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAANTGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTtgct > 1:140352/1‑299 (MQ=255) aTTCAACAAAGTCTGGCATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCagaaga < 1:77835/300‑1 (MQ=255) tCAACGAAGTCTGGCATATCGAGGGCGGAATTATTGACTACGTCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGTAAGAGTTTTGTTGTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACTAGTGCGGAGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATGCAGTGTCCAGTATGCGCGTAAAAATACAAGAGGT‑GTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAAc < 1:373464/300‑1 (MQ=255) aacaaAGTCTGGCATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAg < 1:229827/300‑1 (MQ=255) gTCTGGCATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCTAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGc > 1:368854/1‑300 (MQ=255) attGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAANTTTTGTTTTTGACGAGCGGGTGGGCGAACGTATATCGGATTAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAAc < 2:555248/268‑1 (MQ=255) attGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGGTGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAAc > 1:555248/1‑268 (MQ=255) attGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAAc > 1:206151/1‑268 (MQ=255) attGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAAc < 2:206151/268‑1 (MQ=255) cGCCCGTAAGGCGCGCNAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCNACGCCGTCGGGCAGGACGNGAAAATGGCAATGAGAt > 1:582409/1‑300 (MQ=255) cGCCCGTAAGGCGCGCGAGTAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGAt > 1:609129/1‑300 (MQ=255) cccGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGTGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAC‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAGGATCt > 2:64937/1‑300 (MQ=255) cccGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGNTTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCt > 1:144130/1‑300 (MQ=255) cccGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCt > 2:35698/1‑300 (MQ=255) cccGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTCGTTTTTGACGAGCGGACGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTCAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTACAGTATGCGCGGAAAAATACAC‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATACGATCt > 2:9944/1‑300 (MQ=255) gcgcgcTAGCAGGGCTTGCCGGTTCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAg < 1:381085/300‑1 (MQ=255) gcgcgcGATCAGTGCTTGCCTGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGGGAAAATGGCAATAAGATCTTTAATAAg < 1:379834/300‑1 (MQ=255) gcgcgcGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAg < 1:379102/300‑1 (MQ=255) gAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGt > 1:683581/1‑300 (MQ=255) aGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATTAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTTTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGAc < 1:381893/300‑1 (MQ=255) aGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGAc < 1:381789/300‑1 (MQ=255) gggCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGTGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTCAACATGATGGCTGCCAACTGCTGTTTATCCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTATAGTGAGATTTGCTGCGACGAAAGCGCGTTACCGCAAGAAGAACAGCGACACCGTCAGGTAGGACGTGAAAATGGCAATAAGATCTTTAATAAGtct > 2:63543/1‑292 (MQ=255) gggCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGa > 2:63429/1‑298 (MQ=255) gggCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGAc > 1:237724/1‑299 (MQ=255) gggCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGAAGCCGTCGGGCAGGACGTGAAAATGGCAATAAAATCTTTAATAAGTCTCGTGGAc > 2:128669/1‑299 (MQ=255) ggTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATTAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAAt < 1:636490/298‑1 (MQ=255) ggTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAAt < 1:636494/298‑1 (MQ=255) ggTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATac < 1:51511/300‑1 (MQ=255) ggTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGGAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAAt < 1:636691/298‑1 (MQ=255) gCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATTAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATacaac < 1:634005/300‑1 (MQ=255) gTTTTATTTGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATTAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAacac < 1:624646/300‑1 (MQ=255) gTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCATAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGACTTTTGATAAGTCTCGTGGACGTCTGAATACAacac < 1:374324/300‑1 (MQ=255) gTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAacac < 1:10759/300‑1 (MQ=255) ttttATTGGCAAAAATTTTGTTGTTGACGAGCGGAGGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAacac < 1:243763/299‑1 (MQ=255) aaaaaTTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATTAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTcc < 2:688936/299‑1 (MQ=255) ttGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAacacctgtctcttatacacatctcgcag < 1:162303/300‑25 (MQ=255) cGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGAt > 2:678667/1‑297 (MQ=255) cGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTNGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTACTAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGc > 2:501638/1‑299 (MQ=255) tatCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGcc < 1:115765/300‑1 (MQ=255) ggATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATcc < 1:607205/300‑1 (MQ=255) gATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATcc > 1:628366/1‑299 (MQ=255) agaTTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTgtcaccaggcaa > 2:327088/1‑289 (MQ=255) gaTTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGTCAAc > 2:154464/1‑300 (MQ=255) gaTTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAAc > 2:155056/1‑300 (MQ=255) gcATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACg < 1:162637/299‑1 (MQ=255) tGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTAc > 1:484847/1‑300 (MQ=255) aGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTctgc < 1:600201/299‑1 (MQ=255) gTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTTAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTctgctg < 2:643416/300‑1 (MQ=255) gTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCTAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTctgctg < 2:449932/300‑1 (MQ=255) gTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTctgctg < 2:366864/300‑1 (MQ=255) gcgcgcCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGc < 1:645711/300‑1 (MQ=255) gTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCAAGCTTACTTCTGCTGTACGCCTTCCa > 2:560915/1‑299 (MQ=255) gCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAaataa > 1:181056/1‑300 (MQ=255) gCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATCGCAGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTACACTGAaataa > 2:295742/1‑300 (MQ=255) cATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATc > 2:677991/1‑300 (MQ=255) cATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATc > 1:327411/1‑300 (MQ=255) cTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCa > 1:251888/1‑299 (MQ=255) gTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACtt < 1:35698/300‑1 (MQ=255) gTAAAAATGATGGCTGCCACCTACTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACtt < 1:64937/300‑1 (MQ=255) gTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAGCACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAAt > 1:447044/1‑296 (MQ=255) gTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAAt > 1:176442/1‑296 (MQ=255) aTTCAGTGTACAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAGGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCATGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCCTCCGGCAACATCTCACGCTTACTTCCGCTGTACGCCTTCCACTGAAATAATCAGATCCCCCTCTTGAGAAGCTGGACCTAAATCTGCCtt > 2:295919/1‑299 (MQ=255) gtgtCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATAtt > 2:303054/1‑300 (MQ=255) gtgtCCAGTATGCGCGGAAAAATACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATAtt > 1:540583/1‑300 (MQ=255) aaTACAA‑AAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACACTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTTAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAAttt < 2:197632/300‑1 (MQ=255) | GTGAAAAGGCCAGTGCCTGGATGAAACATAACGGATTCAACAAAGTCTGGCATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAA‑AGGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTT > NC_000913/1117423‑1117998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |