Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,114,389	C→T	A368T (GCA→ACA)	mdtG ←	efflux pump MdtG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,114,389	0	C	T	98.8%	281.0 / NA	85	A368T (GCA→ACA)	mdtG	efflux pump MdtG
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (36/48);  total (37/48)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGGCGTCGATGCCGGGGTTGTCTGCAAGAAATTCTTCGCGTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGC  >  NC_000913/1114098‑1114680                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      ttCGGCGTCGATGCCGGGGTTGTCTGCAAGAAATTCTTCGCGTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATc                                                                                                                                                                                                                                                                                                <  1:688486/299‑1 (MQ=255)        tCGATGCCGGGGTTGTCTGCAAGAAATTCTTCGCGTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTcc                                                                                                                                                                                                                                                                                        <  1:636841/300‑1 (MQ=255)           aTGCAGGGGTTGTCTGCAAGAAATTCTTCGCGTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGt                                                                                                                                                                                                                                                                                     >  1:607724/1‑300 (MQ=255)             gCCGGGGTTGTCTGCAAGAAATTCTTCGCGTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCTGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTaa                                                                                                                                                                                                                                                                                   <  2:22390/300‑1 (MQ=255)                ggggTTGTCTGCAAGAAATTCTTCGCGTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGTAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGGAGACTGTTCCATGAATAGACTGCGGTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGt                                                                                                                                                                                                                                                                                <  1:461446/300‑1 (MQ=255)                ggggTTGTCTGAAAGAAATCCTTCGCGTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTCTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGACTAACACTC‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑TCCTGACACAGTAGTTCGCTGACATCGCTGTTCCCACCAATGATCAGGTAAc                                                                                                                                                                                                                                                                                  >  2:86734/1‑298 (MQ=255)                 gggTTGTCTGCAAGAAATTCTTCGCGTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGt                                                                                                                                                                                                                                                                                <  1:459113/299‑1 (MQ=255)                      gTCTGCAAGAAATTCTTCGCGTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGAAGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCCGAAACCGTAATTCGCTGAAATCGCTGTTCCCATCACTGGTCCGGtca                                                                                                                                                                                                                                                                                   >  2:113935/1‑289 (MQ=255)                                          gTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGtt                                                                                                                                                                                                                                                      <  1:479663/299‑1 (MQ=255)                                           tGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTtat                                                                                                                                                                                                                                                    <  1:483573/300‑1 (MQ=255)                                              aGCGTTAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATGGCTGATTNTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCA‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTTGTTATAGc                                                                                                                                                                                                                                                 <  2:548280/300‑1 (MQ=255)                                              aGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGc                                                                                                                                                                                                                                                 <  1:244413/300‑1 (MQ=255)                                               gCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCt                                                                                                                                                                                                                                                >  1:53553/1‑300 (MQ=255)                                                 gTCAATGGCTTCTTCACGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTACGCTTTTGCCAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCAATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CCCTGAAACCGTAGTTCACCGAAATCGCTGTTCCCATCAATGGTCCGGTGACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGa                                                                                                                                                                                                                                              >  2:135763/1‑300 (MQ=255)                                                    aaTGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CACCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAg                                                                                                                                                                                                                                            <  2:129363/299‑1 (MQ=255)                                                     attgCTTCTTCAAGCGTTGCGGACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTTGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAGGAt                                                                                                                                                                                                                                          <  2:385809/297‑1 (MQ=255)                                                      tggattcttcAAGCGTTGCGTACATGGTCATCAAGATTCTCACTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGGATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTANTTCGCTGAAATCGCTGTTCCCATCCATGGTCCGGCAACGTTGCAAATATCACGAAACGA‑TTGGTTATAGCTGAAgcta                                                                                                                                                                                                                                         >  1:143465/5‑297 (MQ=255)                                                      tGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTANTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATa                                                                                                                                                                                                                                         >  1:501926/1‑300 (MQ=255)                                                            cttcAAGCGTTGCGTACATGGTCATCACGATTATCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCAATCAATGGTCCGGTAACGTTGCCAATATCCCGAAACGC‑TCGGTTATAGCTGAAGATACGccc                                                                                                                                                                                                                                    >  2:99914/1‑299 (MQ=255)                                                                 aGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCAAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGAt                                                                                                                                                                                                                              >  2:661019/1‑300 (MQ=255)                                                                    gTTGCGTACATGGTCATCACGATTCTCCATTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCAAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCTGGCGATCt                                                                                                                                                                                                                            >  2:204458/1‑299 (MQ=255)                                                                     ttGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCt                                                                                                                                                                                                                            >  2:611978/1‑298 (MQ=255)                                                                      tGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGt                                                                                                                                                                                                                         <  1:204458/300‑1 (MQ=255)                                                                       gCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGtt                                                                                                                                                                                                                        >  1:534369/1‑300 (MQ=255)                                                                       gCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGACAATATCACGAAACGA‑CTGGTTATCGCTGAAGATACGCCCGGCGATCTGGtt                                                                                                                                                                                                                        >  2:58416/1‑300 (MQ=255)                                                                       gCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAACCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGACAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCACATCACTGGTCTGGTAGCGTTGCCAATATCACGAAGCGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGtt                                                                                                                                                                                                                        >  2:58424/1‑300 (MQ=255)                                                                         gTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTNAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCAAGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGc                                                                                                                                                                                                                      >  2:538980/1‑300 (MQ=255)                                                                         gTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGc                                                                                                                                                                                                                      >  2:4879/1‑300 (MQ=255)                                                                         gTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCAATGAATAGACTGCGTTGAATAACACTA‑‑AGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTNAAATCGCTGTTCCAATCAATGGTCCGGTAACGTTGCAAATCTCAAGAAACGA‑GTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGc                                                                                                                                                                                                                      >  2:538843/1‑300 (MQ=255)                                                                                    tcaCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTaa                                                                                                                                                                                                           >  1:42288/1‑300 (MQ=255)                                                                                       cGATTCTCCATTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGCGTTGTAAAca                                                                                                                                                                                                        >  2:361239/1‑299 (MQ=255)                                                                                            ctcCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTCGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCACTATCACGAAACGA‑CTGGTTATAGCTGAAGATACGCCCGGTGATCTGGTTGCTCGAGTTGTAAACCAACa                                                                                                                                                                                                    >  2:123373/1‑299 (MQ=255)                                                                                            ctcCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACAACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGCAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCGGGTTGCTCGAGTTGTAAACCAACa                                                                                                                                                                                                    >  2:121419/1‑299 (MQ=255)                                                                                            ctcCCTTTGAACTGATGAGGCTTCAGGGAAAAGGATAGCTGACTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGACCTGGTTGCTCGAATTGTAAACCAACa                                                                                                                                                                                                    >  2:121673/1‑299 (MQ=255)                                                                                             tcCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAg                                                                                                                                                                                                   <  1:203311/299‑1 (MQ=255)                                                                                                    gAGTTGATGAGGTTTCATGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTa                                                                                                                                                                                           <  1:289249/300‑1 (MQ=255)                                                                                                       ttGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTAcgg                                                                                                                                                                                        <  2:465135/300‑1 (MQ=255)                                                                                                               gTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGNTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGagt                                                                                                                                                                                >  2:503494/1‑300 (MQ=255)                                                                                                                    aGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGc                                                                                                                                                                           <  1:441334/300‑1 (MQ=255)                                                                                                                      ggAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCAc                                                                                                                                                                         <  1:613266/300‑1 (MQ=255)                                                                                                                               tAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCGGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATcggcg                                                                                                                                                                 <  2:56716/299‑1 (MQ=255)                                                                                                                                   tGATTCTCCGCTTTTGCAAGTATGAAATGCGAAAAATCAGTTCGATACCGGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACTAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACTAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCAc                                                                                                                                                             <  1:285092/299‑1 (MQ=255)                                                                                                                                   tGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCAc                                                                                                                                                             <  1:284432/299‑1 (MQ=255)                                                                                                                                    gATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCAc                                                                                                                                                             <  2:239851/298‑1 (MQ=255)                                                                                                                                                                    aaaTCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACTAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGTGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATgg                                                                                                                           <  2:81595/300‑1 (MQ=255)                                                                                                                                                                             cGATCCCTGGGGTATTCGACGACGCCGTAGCCTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGCGAA‑ATACCG‑CTCTGAAACCGTCGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCACTATCACGCAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCCAGTTGCAAGGGAGTCTGAAc                                                                                                                  >  2:302716/1‑300 (MQ=255)                                                                                                                                                                                           ttCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGTGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGa                                                                                                    <  2:96850/300‑1 (MQ=255)                                                                                                                                                                                                    gacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCCACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCAAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAAcagca                                                                                           >  2:421033/1‑300 (MQ=255)                                                                                                                                                                                                    gacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAAcagca                                                                                           >  1:23805/1‑300 (MQ=255)                                                                                                                                                                                                    gacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAAcagc                                                                                            <  2:132212/299‑1 (MQ=255)                                                                                                                                                                                                    gacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCAAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAAcagc                                                                                            >  2:694401/1‑299 (MQ=255)                                                                                                                                                                                                    gacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCCGTACGACGGGGAGTAGTGCACCATCGGCAGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCACTGGCGTCTGAACGTAAGAAAGTGGGATCAAcagca                                                                                           >  2:147847/1‑300 (MQ=255)                                                                                                                                                                                                    gacgTAGACTGTTCCATGAATAGACGGCGTTGAATAACACTA‑‑CGCCAGCGTTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAAcagc                                                                                            <  2:640411/299‑1 (MQ=255)                                                                                                                                                                                                        tAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑c                                                                                      <  2:607724/300‑1 (MQ=255)                                                                                                                                                                                                        tAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑c                                                                                      <  1:68414/300‑1 (MQ=255)                                                                                                                                                                                                         aGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑c                                                                                      <  1:491637/299‑1 (MQ=255)                                                                                                                                                                                                         aGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑Ca                                                                                     >  2:624965/1‑300 (MQ=255)                                                                                                                                                                                                             tGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGaaa                                                                                 <  2:72419/300‑1 (MQ=255)                                                                                                                                                                                                             ggTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACTATAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGaaa                                                                                 <  2:72454/299‑1 (MQ=255)                                                                                                                                                                                                                tCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATNGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTa‑cagaacaga                                                                              >  2:505978/1‑300 (MQ=255)                                                                                                                                                                                                                   aTGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATc                                                                            <  2:669232/299‑1 (MQ=255)                                                                                                                                                                                                                    tGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCTGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCa                                                                           >  1:247156/1‑299 (MQ=255)                                                                                                                                                                                                                    tGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAg                                                                          <  1:410619/300‑1 (MQ=255)                                                                                                                                                                                                                       aTAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAgcgc                                                                       <  1:646348/300‑1 (MQ=255)                                                                                                                                                                                                                             gcgcgcgAATAACACTANGCGCCAGCGGGGAAGAGANGATACCGGATCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGAGTTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTANCAGAAAAGATCAgcgc                                                                       <  1:579074/294‑1 (MQ=255)                                                                                                                                                                                                                              gCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATc                                                                <  1:665287/300‑1 (MQ=255)                                                                                                                                                                                                                                ctgcgagatgtgtataagagacaggTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATc                                                                >  1:249418/24‑300 (MQ=255)                                                                                                                                                                                                                                   gAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGAt                                                           >  2:438334/1‑300 (MQ=255)                                                                                                                                                                                                                                   gAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTAAGGCGGGGAGTAGTGCACCATCGGCGGCACAGAGCAAAAAACGTAAAATCCAAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGGTCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGAt                                                           >  2:599068/1‑300 (MQ=255)                                                                                                                                                                                                                                   gAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGACAATATCAAGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGAt                                                           >  1:354795/1‑300 (MQ=255)                                                                                                                                                                                                                                        acacTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCTGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCtttt                                                      <  1:460076/300‑1 (MQ=255)                                                                                                                                                                                                                                        acacTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCGGGATCtttt                                                      >  2:321162/1‑300 (MQ=255)                                                                                                                                                                                                                                        acacTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCtttt                                                      >  1:298989/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGAtt                                           <  1:661019/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGTTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCTGCGATCTGTTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCAc            <  2:475284/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTTGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCAc            <  1:86734/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aGTTCGCTGAAAGCGCTGTTCCCGTCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTTAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCGAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGACCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCAc            <  2:107611/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGc       <  1:538980/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             tGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCag     <  1:661464/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gAAATCGCTGTTCCCATCAATGGTTCGGTAACGTTGCCAGTATCACGAAACGA‑GTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATTGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCGGTAATCAGGATCTCTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGGGCACTTAGc       <  1:538843/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATACCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCNGCGATCTGGTTGCTCGATTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCag     <  1:135763/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                aaTCGCTGTTCCCATCAATGGTCTGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCTGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAgc  <  1:123373/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                aaTCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGTCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAgc  <  1:121419/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                aaTCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAgc  <  1:121673/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                aaTCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACAAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAgc  <  1:361239/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      TTCGGCGTCGATGCCGGGGTTGTCTGCAAGAAATTCTTCGCGTGCAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTA‑‑CGCCAGCGGTGACGAGAA‑ATACCG‑CTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGA‑TTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGCAAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTA‑CAGAAAAGATCAGCGCTGTAATCAGGATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGC  >  NC_000913/1114098‑1114680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑