Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,110,033	G→A	Q233Q (CAG→CAA)	opgG →	osmoregulated periplasmic glucans biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,110,033	0	G	A	100.0%	193.2 / NA	61	Q233Q (CAG→CAA)	opgG	osmoregulated periplasmic glucans biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/30);  total (31/30)

GATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTAC  >  NC_000913/1109761‑1110321                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                  gATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTc                                                                                                                                                                                                                                                                        >  1:58880/1‑300 (MQ=255)                    tCAGCATGCTCGGTGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGTCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCAc                                                                                                                                                                                                                                                     <  2:246332/300‑1 (MQ=255)                    tCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCAc                                                                                                                                                                                                                                                     <  2:74173/300‑1 (MQ=255)                       gCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGt                                                                                                                                                                                                                                                  <  1:104442/300‑1 (MQ=255)                            cTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAAcc                                                                                                                                                                                                                                             >  2:697639/1‑300 (MQ=255)                             tCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAAcc                                                                                                                                                                                                                                             <  1:115865/299‑1 (MQ=255)                               ggggCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAg                                                                                                                                                                                                                                           <  1:634546/299‑1 (MQ=255)                               ggggCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGAGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAg                                                                                                                                                                                                                                           <  1:111129/299‑1 (MQ=255)                                gggCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTa                                                                                                                                                                                                                                         >  1:681753/1‑300 (MQ=255)                                gggCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATATGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAg                                                                                                                                                                                                                                           <  1:83967/298‑1 (MQ=255)                                  gCCAGCTATTTCCGCGTGATTGTTGCATGTCAGGTTGATGGCCTTTCTGCCCGCGGCCCGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑TCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAATCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCTTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGTGCGATAAAGTCGGCAAACGGGGGGTTGCAc                                                                                                                                                                                                                                                     <  2:73855/286‑1 (MQ=255)                                     aGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGt                                                                                                                                                                                                                                      <  1:600734/298‑1 (MQ=255)                                     aGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTc                                                                                                                                                                                                                                    >  1:357104/1‑300 (MQ=255)                                     aGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTc                                                                                                                                                                                                                                    >  2:18607/1‑300 (MQ=255)                                     aGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGAAACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACAAGTCTGTTc                                                                                                                                                                                                                                    >  2:16678/1‑300 (MQ=255)                                      gCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTcc                                                                                                                                                                                                                                   <  1:348328/300‑1 (MQ=255)                                         aTTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGTTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTc                                                                                                                                                                                                                                    >  1:97639/1‑296 (MQ=255)                                                 gTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGcc                                                                                                                                                                                                                         <  1:179100/299‑1 (MQ=255)                                                  tGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGa                                                                                                                                                                                                                       >  1:356392/1‑300 (MQ=255)                                                   gATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGaa                                                                                                                                                                                                                      >  1:482871/1‑300 (MQ=255)                                                        gTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTNTGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGaaccaac                                                                                                                                                                                                                 >  2:511909/1‑300 (MQ=255)                                                        gTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGaaccaac                                                                                                                                                                                                                 >  1:459285/1‑300 (MQ=255)                                                                  ggtTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCACGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGc                                                                                                                                                                                                       >  2:323823/1‑300 (MQ=255)                                                                                            tGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCAc                                                                                                                                                                             >  1:162737/1‑299 (MQ=255)                                                                                            tGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCAc                                                                                                                                                                             <  1:234913/299‑1 (MQ=255)                                                                                               aatcggATACCGCCTTGNCCATCGGGTGAAGAATTTNCCGCGCTCTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTANTCGTCCGGAGTTGCAc                                                                                                                                                                             <  1:579990/295‑1 (MQ=255)                                                                                               aagcggATACCGCCTTGNCCATCGGGTGAAGAATTTNCCGCGCTCTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTANTCGTCCGGAGTTGCAc                                                                                                                                                                             <  1:580125/295‑1 (MQ=255)                                                                                                              tG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTa                                                                                                                                                          <  2:445453/300‑1 (MQ=255)                                                                                                                   aTCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCTACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATc                                                                                                                                                        <  2:135003/298‑1 (MQ=255)                                                                                                                   aTCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGANAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCTGAGTTGCACGACTCTAACGGTCTCTCTATCCa                                                                                                                                                      <  2:513401/300‑1 (MQ=255)                                                                                                                   aTCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATcc                                                                                                                                                       <  1:29677/299‑1 (MQ=255)                                                                                                                   aTCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTATATcc                                                                                                                                                       <  1:383284/299‑1 (MQ=255)                                                                                                                    tCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTACGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCa                                                                                                                                                      <  2:488751/299‑1 (MQ=255)                                                                                                                                 tt‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCg                                                                                                                                        <  1:420788/300‑1 (MQ=255)                                                                                                                                                agagTTCTGGATCGAGCGTCCAAAACCGACCGAGAAACGTTTAACCATCTATGCATTGCTGGACTCGCCGCGTGCGACAGGTGCTTACAAATTCTTGGTTATGCCAGGGCGTGACATGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCg                                                                                                                           <  2:581814/300‑1 (MQ=255)                                                                                                                                                   gTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTcc                                                                                                                        >  1:130109/1‑300 (MQ=255)                                                                                                                                                   gTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGAGTTGCACCGTTAACCAGTACGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCAGGTAATGGCGAATGGATTTGTCGTcc                                                                                                                        >  2:111860/1‑300 (MQ=255)                                                                                                                                                        ggATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGa                                                                                                                   >  1:433921/1‑300 (MQ=255)                                                                                                                                                          aTCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGTTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAAt                                                                                                                 <  2:97639/300‑1 (MQ=255)                                                                                                                                                          aTCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAAt                                                                                                                 <  1:632395/300‑1 (MQ=255)                                                                                                                                                                                             cATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCAt                                                                              >  2:484350/1‑300 (MQ=255)                                                                                                                                                                                               tCTATGCATTGCTTGACTCGCCGCGTGTGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTCAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCAGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCAt                                                                              >  2:483196/1‑298 (MQ=255)                                                                                                                                                                                               tCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGACCCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGATAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGACACATTTAGCGGTCAGCAGCTTCTACAt                                                                              >  2:437015/1‑298 (MQ=255)                                                                                                                                                                                               tCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TTGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACACGAAACACTTAGCGGTAAGCAGCTTCTCCAt                                                                              >  2:271357/1‑298 (MQ=255)                                                                                                                                                                                                       ttGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGACCGAACCAACCGTCGCATGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTATCTATCCATGCCGGTAATGGCGAATAGATCTGGAGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGTAGCTTCTACATGGAAAAcc                                                                      >  2:88469/1‑298 (MQ=255)                                                                                                                                                                                                                     gcgTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTc                                                       <  1:610412/299‑1 (MQ=255)                                                                                                                                                                                                                       gTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGTGCTTGACACGTTTGTGGATGTGCAATCGAAAATCTACCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGTTAATTGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTg                                                     <  2:159462/299‑1 (MQ=255)                                                                                                                                                                                                                              aGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAgcg                                             <  1:262690/300‑1 (MQ=255)                                                                                                                                                                                                                              aGGTGCTGACAAATTCGTGGTTATACCAGGGCTTGACACGGTTGTGGATGTGCAATCGAAAATCTACCTGCTCGATAAAGTCGGCAAACTGTGGTTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGATGCAgcg                                             <  2:58880/300‑1 (MQ=255)                                                                                                                                                                                                                                gTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGc                                                 <  1:487896/294‑1 (MQ=255)                                                                                                                                                                                                                                gTGCTTACAAATTCGTGGTTATGCCACGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAgcgc                                            >  1:125414/1‑299 (MQ=255)                                                                                                                                                                                                                                                ggTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTccc                            >  2:696375/1‑299 (MQ=255)                                                                                                                                                                                                                                                      gCCAGGGCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGTTAAAGTCAGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTACATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTACCGCTTTGa                     >  1:681251/1‑300 (MQ=255)                                                                                                                                                                                                                                                           ggCGTGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTACCGCTTTGAAGAtc                >  1:432206/1‑300 (MQ=255)                                                                                                                                                                                                                                                               tGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTACATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCga            >  2:467678/1‑300 (MQ=255)                                                                                                                                                                                                                                                               tGACACGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGAGGTCAGCAGCTTCTACATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCga            >  2:163052/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 acacGGTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTGGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCgatg          <  2:459285/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     ggTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATc       >  1:31976/1‑299 (MQ=255)                                                                                                                                                                                                                                                                      gTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGt     >  2:70746/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gTTGTGGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGt     >  1:204002/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gtgGATGTGCAATCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTAc  >  1:685096/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                  GATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTG‑CCATCGGGTGAAGAATTT‑CCGCGCT‑TCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGTGCTTACAAATTCGTGGTTATGCCAGGGCGTGACACGGTTGTGGATGTGCAGTCGAAAATCTATCTGCGCGATAAAGTCGGCAAACTGGGGGTTGCACCGTTAACCAGTATGTTCCTGTTTGGGCCGAACCAACCGTCGCCTGCAAATAACTA‑TCGTCCGGAGTTGCACGACTCTAACGGTCTCTCTATCCATGCCGGTAATGGCGAATGGATCTGGCGTCCGTTGAATAACCCGAAACATTTAGCGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTAC  >  NC_000913/1109761‑1110321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑