Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	780,624	C→T	noncoding (71/76 nt)	lysT →	tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	780,624	0	C	T	97.3%	230.5 / NA	75	noncoding (71/76 nt)	lysT	tRNA‑Lys
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (40/32);  minor base G (0/2);  total (41/34)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGTAAATACCCTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACC  >  NC_000913/780328‑780913                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                     tCAGTAAATACCCTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACtcac                                                                                                                                                                                                                                                                                                   >  1:667825/1‑300 (MQ=255)    gTAAATACCCTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGAACAGAAGTCGCATTATTTATGGTCGTGTCGTGCGAATCATACGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGGTATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAATTGAATTTTAATCAATTGGTCGCAAGTTCGAATCCTGCACGACTCACCaa                                                                                                                                                                                                                                                                                                >  2:253766/1‑300 (MQ=255)     tAAATACCCTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAAt                                                                                                                                                                                                                                                                                               <  2:347001/300‑1 (MQ=255)         tACCCTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCt                                                                                                                                                                                                                                                                                           <  1:650193/300‑1 (MQ=255)           cccTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGccaa                                                                                                                                                                                                                                                                                         >  2:278405/1‑297 (MQ=255)           cccTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTaa                                                                                                                                                                                                                                                                                         >  1:697915/1‑300 (MQ=255)           cacTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGTAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTaa                                                                                                                                                                                                                                                                                         >  2:170770/3‑300 (MQ=255)                    tGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑c                                                                                                                                                                                                                                                                               <  1:331744/300‑1 (MQ=255)                    tGATGGCGCTAAACAGGCACAAAAACGTCTAAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑c                                                                                                                                                                                                                                                                               <  2:67507/300‑1 (MQ=255)                     gATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑c                                                                                                                                                                                                                                                                               <  2:26380/299‑1 (MQ=255)                         gcgcTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGgta                                                                                                                                                                                                                                                                           >  1:208035/1‑299 (MQ=255)                         gcgcTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGgt                                                                                                                                                                                                                                                                            >  2:286536/1‑298 (MQ=255)                         gcgcTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCAGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGgta                                                                                                                                                                                                                                                                           >  2:124143/1‑299 (MQ=255)                             tAAATAGGCACAAAAACTTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGTTCTTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGGCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGTTCGTTAGCTCAGTTGGTAGATCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGTTGGAAG‑CGGTAGTaa                                                                                                                                                                                                                                                                       <  2:275948/299‑1 (MQ=39)                             gaaaCAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTaa                                                                                                                                                                                                                                                                       <  2:44019/298‑1 (MQ=255)                              aaaCAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGTTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACccaccagttttaacatcgaagacagatgttaag                                                                                                                                                                                                                                                                      <  2:665930/300‑28 (MQ=38)                                              gTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGt                                                                                                                                                                                                                                                      <  2:431982/299‑1 (MQ=255)                                                         aTGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAANTCTGAAATCAGTAATATATGCCGCCGTTGCCANGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCaa                                                                                                                                                                                                                                           >  2:520891/1‑299 (MQ=255)                                                         aTGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAGCCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAAc                                                                                                                                                                                                                                          >  1:354605/1‑300 (MQ=255)                                                         aTGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGAAAG‑CGGTAGTCAAACGTGAAGGATAACGTTGCCTGAGCaa                                                                                                                                                                                                                                           >  2:113728/1‑299 (MQ=255)                                                              aTGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGGAGGATAACGTTGCATGAGCAACGGccc                                                                                                                                                                                                                                     >  2:234678/1‑300 (MQ=255)                                                                gCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGCCCTCCCCCAACCCGCCCCCCCCTCCCAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGa                                                                                                                                                                                                                                   >  1:91147/1‑300 (MQ=37)                                                                  aTAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTTGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAg                                                                                                                                                                                                                                 <  2:472839/300‑1 (MQ=255)                                                                     acacacGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCGGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTCAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTNCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGaa                                                                                                                                                                                                                                  >  1:146995/1‑296 (MQ=255)                                                                     acacacGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTNCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGc                                                                                                                                                                                                                              >  1:146956/1‑300 (MQ=255)                                                                     acacacGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGc                                                                                                                                                                                                                              >  1:96823/1‑300 (MQ=255)                                                                     acacacGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCGAAGGTGGAAA‑CAGAGGTAAAACGTGGAGGATAACGTTGCATAAGCAACGGCCCGCAGGGc                                                                                                                                                                                                                              >  2:191848/1‑300 (MQ=39)                                                                     acacacGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGACGTTGCCAAGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTNCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGc                                                                                                                                                                                                                              >  1:149051/1‑300 (MQ=255)                                                                            aCCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGATCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGa                                                                                                                                                                                                                       >  1:632642/1‑300 (MQ=255)                                                                                gAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGGAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCgagctga                                                                                                                                                                                                                       >  2:653879/1‑292 (MQ=255)                                                                                gAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGT‑TTTAACATCGAAGACGGATGTCAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCgagcgga                                                                                                                                                                                                                       >  2:653870/1‑292 (MQ=18)                                                                                                tgttcgtgtcgtgCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                                                                                                                                                                                                     <  2:292309/295‑1 (MQ=255)                                                                                                tgtgcgtgtcgtgCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTTTTGCGCTCAAGTCTGAAATCATTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                                                                                                                                                                                                     <  2:1047/294‑1 (MQ=255)                                                                                                tGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGAc                                                                                                                                                                                                  <  2:697915/299‑1 (MQ=255)                                                                                                        tcgtgCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCNCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCcacc‑ac                                                                                                                                                                                          <  1:495009/300‑1 (MQ=255)                                                                                                        tcgtgCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCcacc‑ac                                                                                                                                                                                          <  2:696432/300‑1 (MQ=255)                                                                                                        tcgtgCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCcacc‑ac                                                                                                                                                                                          <  1:522916/300‑1 (MQ=255)                                                                                                        tagtgCGCATCATAAGCAGGTGAGGGAGCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCGGGAATATATGCCGCCGTGGTTACGGGGTATAAAACAAACCGAAAGCAACGAAAAAGGGGGGCGTTGGATCAGGTGGGAGAGCATGGGACTTTTAAGCAAGTGGGCGCAGGGTTGAAGCCTGCACGACGCACAAACCGCTAAGTTGGAAG‑CGTTAGTAGAACGTGAAGGATAAAGTTGCATGAGCAAAGGCCCTAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCcacc‑ac                                                                                                                                                                                          <  2:632642/298‑1 (MQ=255)                                                                                                           tgCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGNCGCAGGTTCGAATCCTGCACGACTCACCAAttgtgac                                                                                                                                                                                                                                                                                         >  2:211277/1‑198 (MQ=255)                                                                                                           tgCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGNCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑TGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACA‑ACTaa                                                                                                                                                                                       >  2:211617/1‑300 (MQ=255)                                                                                                           tgCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGNCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCACCGGCACGAAGGGCGAGACGAAGCCGAGTCATCCTGCACGAACcacc‑accaa                                                                                                                                                                                       >  2:211940/1‑297 (MQ=255)                                                                                                                 tCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACAtagt                                                                                                                                                                                 >  1:209579/1‑300 (MQ=255)                                                                                                                          gTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAATAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCACGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTgta                                                                                                                                                                         >  1:672303/1‑299 (MQ=255)                                                                                                                          gTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCNTGCACGACTCACCAATCGCGAAGGCGGAAG‑CGGTAGTAAAACGTGCAGGATAACGTTGGATGa                                                                                                                                                                                                                                               >  2:208439/1‑230 (MQ=255)                                                                                                                          gTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTgta                                                                                                                                                                         >  1:97590/1‑299 (MQ=255)                                                                                                                          cttGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATCTGCCGCCGTTGTCACGGGATATCACACAAACCGAAAGCAACGAAACAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCNTGCACGACTCACCAATCGATaa                                                                                                                                                                                                                                                                                         >  2:208385/2‑189 (MQ=37)                                                                                                                                  aTCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATcc                                                                                                                                                                   <  2:489980/297‑1 (MQ=255)                                                                                                                                                       gcgcTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTc                                                                                                                                           <  1:278405/300‑1 (MQ=255)                                                                                                                                                              aGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCt                                                                                                                                       >  1:471404/1‑297 (MQ=255)                                                                                                                                                              aGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGCCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTCGCATCGGGTGATcagctcagct                                                                                                                                       >  2:75563/1‑297 (MQ=255)                                                                                                                                                               gTCTGAAATCAGTAATNTATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGNATCCAGCGTAGTATCGGGNGATTAGCTCAGCt                                                                                                                                       >  1:586683/1‑296 (MQ=255)                                                                                                                                                                  tGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGgaga                                                                                                                                 <  1:85406/299‑1 (MQ=255)                                                                                                                                                                                tatGCCGTCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGTTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACaa                                                                                                                  <  2:377414/300‑1 (MQ=255)                                                                                                                                                                                tatGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACaa                                                                                                                  <  2:614514/300‑1 (MQ=255)                                                                                                                                                                                      gccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAaggag                                                                                                              <  1:344190/298‑1 (MQ=255)                                                                                                                                                                                               aCGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggc                                                                                                     <  1:52129/298‑1 (MQ=255)                                                                                                                                                                                                              aacCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAACCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTGAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGAAGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGATGATTAGCTCAGCTGGGAGAGCACCTACCTTACAAGGAGGGGGTCGGCGGTGCGATCCCGtcatc                                                                                    >  2:572066/1‑300 (MQ=255)                                                                                                                                                                                                               acCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGCTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGCGGAAG‑CGGTAGTAAAACGTGGAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAACCACCCAC‑AACGTAATTAGTTGTAGTATCCAGCGTAGTATCGGGTGGTTAGCTCAGCTGGGAGAGCACCTACCTTACAAGGAGGGGGTCGGGGGTTTGATCCCGtcgtca                                                                                   >  2:203259/1‑300 (MQ=25)                                                                                                                                                                                                                        aaCGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGTAGGGGGTCGGCGGTTCGATCCCGTCATCACCcaccac                                                                           <  1:169522/299‑1 (MQ=255)                                                                                                                                                                                                                         aCGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCgagcgga                                                                                                                                                                                                                       >  2:364044/1‑155 (MQ=255)                                                                                                                                                                                                                            aaaaaGTTGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGTTCGCAGGTTCGAGTCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCTAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTTGTAGAGCACCTCCCTTACAAGTAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGt                                                                     <  2:258322/300‑1 (MQ=37)                                                                                                                                                                                                                            aaaaaGTGTTTCGTTAGCTCAGTTGGTAGAGCATTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAATTATGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGt                                                                     <  2:307715/300‑1 (MQ=255)                                                                                                                                                                                                                            aaaaaGGGGGTCGGTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGTAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGt                                                                     <  2:307703/300‑1 (MQ=255)                                                                                                                                                                                                                                              tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCTAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTag                                                   <  1:272188/300‑1 (MQ=255)                                                                                                                                                                                                                                              tCAGTTGGGAGAGCAGTTGACTTTGAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAATTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTag                                                   <  1:271809/300‑1 (MQ=255)                                                                                                                                                                                                                                              gcggTTGGTAGAGCAGGTGACTTTTAATCGAGTGGTCGCAGGTTCGAATCCTGCACGACTTACCAATCGCTAAGTTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCTTGCGCGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAACACCTCCCTTACAAGTAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTag                                                   <  2:469160/297‑1 (MQ=37)                                                                                                                                                                                                                                                 gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGc                                                <  1:341880/300‑1 (MQ=255)                                                                                                                                                                                                                                                   tGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGgcatcccacac                                                                              >  2:628867/1‑263 (MQ=255)                                                                                                                                                                                                                                                             gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTa                                    >  1:416330/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACTTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTTGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGTTCGGCGGTTCGATCTCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg                                           <  2:75244/287‑1 (MQ=255)                                                                                                                                                                                                                                                                            aTTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCCAAGGTGAAAG‑CAGTAGTCAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGCAGGGCgagcgaa                                                                                                                                                                                                                       >  2:284766/1‑104 (MQ=255)                                                                                                                                                                                                                                                                                             aTCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGtt                                                                 >  1:372586/1‑239 (MQ=255)                                                                                                                                                                                                                                                                                              tCCTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAc   <  1:286536/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tCCTGCACGACGCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTGGCATGAGCAACGACCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGACCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAc   <  2:641861/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                cTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTACCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGaac  >  1:89362/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                                cTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCNGGGAGAGCACCTCCATTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccca                                                                               >  2:208012/1‑223 (MQ=255)                                                                                                                                                                                                                                                                                                cTGCACGACTCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTACCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGa    >  2:615829/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                     TCAGTAAATACCCTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAG‑CGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACC‑ACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACC  >  NC_000913/780328‑780913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑