Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	81,102	G→A	S286L (TCG→TTG)	leuB ←	3‑isopropylmalate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	81,102	0	G	A	100.0%	280.6 / NA	82	S286L (TCG→TTG)	leuB	3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/39);  total (43/39)

TTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGT  >  NC_000913/80814‑81396                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                       ttCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCTCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCcgcccgcc                                                                                                                                                                                                                                                                                             >  1:492119/1‑300 (MQ=255) ttCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCcgcccgcc                                                                                                                                                                                                                                                                                             >  1:239337/1‑300 (MQ=255) ttCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCAAAGCcgcccgcc                                                                                                                                                                                                                                                                                             >  1:559294/1‑300 (MQ=255) ttCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTATGCTACATAGCGGGCAATGATATCGCACATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCACGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATTTGGTGCCAAgccgc                                                                                                                                                                                                                                                                                                  >  2:1063/1‑295 (MQ=255)          aaCGTGAGCTTCTAACAATTTTTCGTGTAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCGAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCTCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGACTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGTTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATAc                                                                                                                                                                                                                                                                                    <  2:474170/300‑1 (MQ=255)          aaCGTGAGCGTCGAACAATTTTTTGTATAATGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGTGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGTGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATAc                                                                                                                                                                                                                                                                                    <  2:92626/300‑1 (MQ=255)          aaCGTGAGCGTCGAACAATTTTTCGTATAACTTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATAc                                                                                                                                                                                                                                                                                    <  1:648866/300‑1 (MQ=255)          aaCGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATAc                                                                                                                                                                                                                                                                                    <  2:348985/300‑1 (MQ=255)          aaCGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATAc                                                                                                                                                                                                                                                                                    <  1:80654/300‑1 (MQ=255)          aaCGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATANCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATAc                                                                                                                                                                                                                                                                                    <  1:138910/300‑1 (MQ=255)          aaCGTGAGCGTCGAACAATTTTGCGTATAACGTCTTAGCCATGATGACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACTGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCCCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATAc                                                                                                                                                                                                                                                                                    <  2:473933/300‑1 (MQ=255)          aaCGTAAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCCTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCNAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATAc                                                                                                                                                                                                                                                                                    <  2:213733/300‑1 (MQ=255)               gAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTcc                                                                                                                                                                                                                                                                               <  1:167829/300‑1 (MQ=255)                                     taACGTTTGAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATTATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGTGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGGTAATGGCGCGTTCAATTGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCt                                                                                                                                                                                                                                                          <  2:107664/299‑1 (MQ=255)                                         gTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCgg                                                                                                                                                                                                                                                     <  1:650482/300‑1 (MQ=255)                                               gCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCa                                                                                                                                                                                                                                               >  2:486575/1‑300 (MQ=255)                                                    gATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATc                                                                                                                                                                                                                                          >  1:103877/1‑300 (MQ=255)                                                     aTTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTNCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATcc                                                                                                                                                                                                                                         >  1:149944/1‑300 (MQ=255)                                                     aTTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATcc                                                                                                                                                                                                                                         >  1:423697/1‑300 (MQ=255)                                                               ttCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCg                                                                                                                                                                                                                                   <  2:244164/296‑1 (MQ=255)                                                               ttCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGcc                                                                                                                                                                                                                               >  1:29062/1‑300 (MQ=255)                                                                 cTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCa                                                                                                                                                                                                                              >  1:102192/1‑299 (MQ=255)                                                                 cTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTACAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCAATCGAGCCa                                                                                                                                                                                                                              >  2:334774/1‑299 (MQ=255)                                                                   gCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCCGCGCCACGGGCTAAATCCCAGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGNCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGt                                                                                                                                                                                                                            >  2:507078/1‑299 (MQ=255)                                                                   gCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGt                                                                                                                                                                                                                            >  1:406862/1‑299 (MQ=255)                                                                   gCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCACATCGAGCCAGt                                                                                                                                                                                                                            >  1:406666/1‑299 (MQ=255)                                                                        aTAGCGGGCAATGAGATCGCCCATTTCCTCGGTACTAACTGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATTCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCATGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCTGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCATTGATCAt                                                                                                                                                                                                                      <  2:60931/300‑1 (MQ=255)                                                                                cAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACt                                                                                                                                                                                                              >  1:470854/1‑300 (MQ=255)                                                                                cAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACt                                                                                                                                                                                                              >  2:420040/1‑300 (MQ=255)                                                                                 aaTGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGTGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGTCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACt                                                                                                                                                                                                              <  1:607542/299‑1 (MQ=255)                                                                                  aTGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCg                                                                                                                                                                                                            <  2:196959/300‑1 (MQ=255)                                                                                                      ctgcgagatgtgtataagagacagggCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCaaa                                                                                                                                                                                        >  1:611830/24‑300 (MQ=255)                                                                                                       gTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCAAAAc                                                                                                                                                                                       >  2:236236/1‑300 (MQ=255)                                                                                                            aaCGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCNCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGtt                                                                                                                                                                                  <  1:515962/300‑1 (MQ=255)                                                                                                                    cAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCCGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCATGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCACATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTAGCCAAACAGGTTGGAGc                                                                                                                                                                             >  2:162342/1‑297 (MQ=255)                                                                                                                        gcCACTGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTTGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCTCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCAcagcag                                                                                                                                                                      <  2:23438/300‑1 (MQ=255)                                                                                                                        gcCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCAcagcag                                                                                                                                                                      <  1:107758/300‑1 (MQ=255)                                                                                                                        gcCACAGGCTGAATCTCCGGTGCGAATGCCTTCTTCTAAGTCACGGTTAATGGCGCGTTCAATGGCACAAGCCACATCATCGGCATCCAGGCTTTAACGCAGCAGCAGGGCCGGTGAAAGGATTTGTGCGATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCTAGTGATCATTGCTCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCAcagcag                                                                                                                                                                      <  2:23427/300‑1 (MQ=255)                                                                                                                           aCGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAAc                                                                                                                                                                   <  1:390229/300‑1 (MQ=255)                                                                                                                              ggCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTc                                                                                                                                                                >  1:423206/1‑300 (MQ=255)                                                                                                                                                      ttcttcTAATTCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGTGTTGTCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGAGCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGGCAAACTGTGATGGATCTTTAATc                                                                                                                                          <  2:392205/298‑1 (MQ=255)                                                                                                                                                      ttcttcTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCa                                                                                                                                         >  1:653842/1‑299 (MQ=255)                                                                                                                                                      ttcttaTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGTACAGCAGAACGTCAAACTGTGATGGATCTTTAAtta                                                                                                                                         >  2:206959/1‑297 (MQ=255)                                                                                                                                                       tcttctAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGc                                                                                                                                       <  2:428931/300‑1 (MQ=255)                                                                                                                                                       tcttctAATGCGCGGTTAATGGCGCGTTCAATGGCACAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATc                                                                                                                                          <  2:242671/297‑1 (MQ=255)                                                                                                                                                        cttctAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCt                                                                                                                                      >  2:650021/1‑300 (MQ=255)                                                                                                                                                              aTGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAANCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtgg                                                                                                                                <  2:228589/300‑1 (MQ=255)                                                                                                                                                                  gcgGTTAATGGCGCGTTCAATTGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCTAAAGGATTTGTGCAATCGGGTTTTCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtgg                                                                                                                             <  2:146145/299‑1 (MQ=255)                                                                                                                                                                  gcgGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCTAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCNCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAtggtgg                                                                                                                             <  2:505272/299‑1 (MQ=255)                                                                                                                                                                     gTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCNGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTAGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGtt                                                                                                                         >  2:226115/1‑300 (MQ=255)                                                                                                                                                                     gTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGtt                                                                                                                         >  1:435875/1‑300 (MQ=255)                                                                                                                                                                                gTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACa                                                                                                              >  1:209264/1‑300 (MQ=255)                                                                                                                                                                                gTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCAATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACa                                                                                                              >  1:209270/1‑300 (MQ=255)                                                                                                                                                                                  tCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACata                                                                                                            <  2:622633/300‑1 (MQ=255)                                                                                                                                                                                   cAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACatat                                                                                                           >  1:470001/1‑300 (MQ=255)                                                                                                                                                                                    aaTGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGTGTTGTCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATg                                                                                                          <  2:69382/300‑1 (MQ=255)                                                                                                                                                                                                 gCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACa                                                                                             >  1:12193/1‑300 (MQ=255)                                                                                                                                                                                                  catcatCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACAt                                                                                            >  2:447516/1‑300 (MQ=255)                                                                                                                                                                                                   atcatcGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATc                                                                                           <  1:697696/300‑1 (MQ=255)                                                                                                                                                                                                           cATCTAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGACGGCAACATCCACATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAATAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATTTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCAAGTTCGACATCCGGGTAtt                                                                                   >  2:340480/1‑300 (MQ=255)                                                                                                                                                                                                                 ggCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCTGGTTGTCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTgg                                                                             <  2:167331/300‑1 (MQ=255)                                                                                                                                                                                                                 ggCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGt                                                                                      >  2:330591/1‑291 (MQ=255)                                                                                                                                                                                                                      tAACGCAGCAGCAGGGTCAGCGAAGGGATTTGGGCAAGCTGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGGTCATGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGGTGGAGCACAGCAGAAc                                                                                                                                                                   <  2:422254/209‑1 (MQ=255)                                                                                                                                                                                                                         cGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATATCGCCAAACAGGTTGGAGCACAGCAGAACGTAAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCAGTGGCGAtct                                                                       >  2:35662/1‑298 (MQ=255)                                                                                                                                                                                                                         cGCAGCAGCAGTGCAAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGACAAAAAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTACTCAGCTGCATGGTGGCGTTGTCGATGTACCTATGCGCCAGTTCGAAATCCGAGTATTCAGTGGCGagcgc                                                                      >  2:35277/1‑295 (MQ=255)                                                                                                                                                                                                                           cagcagcagTGCCAGCNAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGNCAGTTCGACATCCGGGTANTCCGTGGCGATCTCGtt                                                                   >  1:579695/1‑300 (MQ=255)                                                                                                                                                                                                                           cagcagcagTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGtt                                                                   >  1:679545/1‑300 (MQ=255)                                                                                                                                                                                                                               agcagTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAAc                                                                <  2:653842/299‑1 (MQ=255)                                                                                                                                                                                                                                gcagTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGa                                                              <  2:478519/300‑1 (MQ=255)                                                                                                                                                                                                                                           gAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCAt                                                   >  2:626229/1‑300 (MQ=255)                                                                                                                                                                                                                                                  ttcgtgCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAATGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATag                                            <  2:29062/297‑1 (MQ=255)                                                                                                                                                                                                                                                              ggTTTGCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCACCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTgcag                                  <  2:492119/298‑1 (MQ=255)                                                                                                                                                                                                                                                               ttttgCGATGTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCAGACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGTTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCAGGGTGGCGTTTTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTGCAGCAc                               <  2:559294/296‑1 (MQ=255)                                                                                                                                                                                                                                                               gTTGGCGATGGTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTGCAGCAc                               <  1:1063/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        gTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGACAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAGTAGAGGATTGCAGCACGTTAGCttt                      >  2:436309/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gTTTTTGCCTGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCACATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACCTCCGGGTATTCCGTGGCGATCTCGTTAACGATCTACCGCCATACCATAGAGGATTGCAGCACGTTGGCttt                      >  2:436351/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 tGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTGCAGCACGTTGGCTTTAtcgatcga             >  1:289536/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 tGCGATATCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCAAATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTGCAGCACGTTGGCTTTAtcgatcga             >  1:621721/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     atatCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTGCAGCACGTTGGCTTTATCGATCGACGTc         <  1:65941/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       atCTGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCTCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTCTTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTGCAGCACGTTGGCTTTATCGATCGACGTCa        >  1:96676/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                          tGGTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTGCAGCACGTTGGCTTTATCGATCGACGTCACtt     <  1:486575/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gTGCCAAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTGCAGCACGTTGGCTTTATCGATCGACGTCACTTtgt  >  1:177441/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                       TTCGTACACAACGTGAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATGATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTTCTAATGCGCGGTTAATGGCGCGTTCAATGGCGCAAGCCGCATCATCGGCATCCAGGCTGTAACGCAGCAGCAGTGCCAGCGAAAGGATTTGTGCAATCGGGTTGGCGATGTTTTTGCCTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAATAGAGGATTGCAGCACGTTGGCTTTATCGATCGACGTCACTTTGT  >  NC_000913/80814‑81396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑