Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	26,666	A→G	T130T (ACA→ACG)	ispH →	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	26,666	0	A	G	100.0%	216.2 / NA	62	T130T (ACA→ACG)	ispH	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/27);  total (35/27)

ATATATGTCCGTCACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTC  >  NC_000913/26373‑26936                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                               atatatGTCCGTCACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGc                                                                                                                                                                                                                                                                          >  2:623722/1‑300 (MQ=255)       gTCCGTCACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCcagtac                                                                                                                                                                                                                                                                    <  1:188325/300‑1 (MQ=255)           gttactgaGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCcagtacag                                                                                                                                                                                                                                                                  <  2:182026/291‑1 (MQ=255)            tcactaagctgtataTAACCGCTATCTGGTCGATAGCTTGCGTGAGCGTGGGGCTATTTTGATTGAGCAGACTAGCGAAGTACCGGACGGCTCGATCCTGACTTTCTCCGCACACGGTGTTTCTCAGGCTGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAATGCGCCCGCGCCAGCCGTCGTGTCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGGACAGTaa                                                                                                                                                                                                                                                               <  2:116557/286‑1 (MQ=255)            tCACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTaa                                                                                                                                                                                                                                                               <  1:192246/300‑1 (MQ=255)                gAAGTGGTACATAACCGCTATGTGGTCAATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCTAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGTTGTTTCTCAGGCGGTACGTAACTAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTaa                                                                                                                                                                                                                                                               <  2:266925/296‑1 (MQ=255)                      gTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGaa                                                                                                                                                                                                                                                        >  1:675657/1‑297 (MQ=255)                      gTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTAAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGCTTCTCATCGGCCACGCCGGGCATCAGGAAGTGGAAGGTACGATGGGCCAGTACAGTAAACCGGaa                                                                                                                                                                                                                                                        >  2:194353/1‑297 (MQ=255)                                         tCGATAGCTTGCGTGGGTGTTGGGCTATCTTTATTGAGCAGATTGTCGAAGTATCGGACGGCGTGATTCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTTACGGTATTTGATGCCATCTGTACGCTGGTGACCAAATTGCAGATGGAAGTCGCCCGCACCAGCCGTCGTGGCGAAGAGTCGATGCTCATCGGCCACGCCGGGCATCCGTAAGTGGAAGGTACGATGGGCcagtacag                                                                                                                                                                                                                                                                  <  2:344207/268‑1 (MQ=255)                                                 ttGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCANATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGANGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGAc                                                                                                                                                                                                                          <  2:530346/300‑1 (MQ=255)                                                         gCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGAtgtgt                                                                                                                                                                                                                   >  1:697687/1‑299 (MQ=255)                                                              gggCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAAc                                                                                                                                                                                                             >  1:213876/1‑300 (MQ=255)                                                              gggCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTACGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCAGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTATAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTATGGAAAc                                                                                                                                                                                                             >  1:213536/1‑300 (MQ=255)                                                                gCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCATACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCTGACGATGTGTGGAAACt                                                                                                                                                                                                            >  1:294075/1‑299 (MQ=255)                                                                       ttATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCa                                                                                                                                                                                                    >  1:26846/1‑300 (MQ=255)                                                                            gAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAANCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAAc                                                                                                                                                                                               <  1:131784/300‑1 (MQ=255)                                                                            gAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTACGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAAc                                                                                                                                                                                               >  2:671152/1‑300 (MQ=255)                                                                                 gATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAaga                                                                                                                                                                                          >  1:158042/1‑300 (MQ=255)                                                                                        gAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGctc                                                                                                                                                                                   <  2:485601/300‑1 (MQ=255)                                                                                           gTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTTACGGTCAAAAACGAAGAGAAGCTCTcc                                                                                                                                                                                >  1:355238/1‑300 (MQ=255)                                                                                                     gcgcGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGNCACGCCGGGCATCCGGAAGTGGAAGATACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCttcataacc                                                                                                                                                                       <  2:497546/299‑8 (MQ=255)                                                                                                              tGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCTGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGc                                                                                                                                                             <  2:294075/300‑1 (MQ=255)                                                                                                                         gCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTNGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGa                                                                                                                                                   <  1:140673/299‑1 (MQ=255)                                                                                                                         gCACACGGGGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAATAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTgta                                                                                                                                                   <  2:297038/299‑3 (MQ=255)                                                                                                                            cacGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTACTTTATGCCCCAGACCACGCTGTCGGTGGATGac                                                                                                                                               >  2:75205/1‑300 (MQ=255)                                                                                                                            cacGGTGCTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTACGCTGGTGACCAAAATGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGAGAAGAGTCGATTCTCATCGGACACGCCGGGCATCCGGAAGTGGAAGGTCCGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAAAGAAGAGAAGCTCTCCtt                                                                                                                                                                              >  2:75224/1‑269 (MQ=255)                                                                                                                            aacGGTGCTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTACTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTACttt                                                                                                                                                                             >  2:75300/2‑270 (MQ=255)                                                                                                                                gtgtTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCTGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGt                                                                                                                                           >  1:81977/1‑300 (MQ=255)                                                                                                                                gtgtTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGt                                                                                                                                           >  1:88044/1‑300 (MQ=255)                                                                                                                                gtgtTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGAAGGTCACAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGt                                                                                                                                           >  1:88228/1‑300 (MQ=255)                                                                                                                                gtgtTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCAGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTAGGTGGATGACACGt                                                                                                                                           >  1:81909/1‑300 (MQ=255)                                                                                                                                  gtTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCt                                                                                                                                         <  1:86581/300‑1 (MQ=255)                                                                                                                                    ttCTCATGCGGTACGTAACGAAGCAAAAAGCCGCGAGTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCTGCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGTAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGTGGGAATGTATCTGTTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAATAGAAGCTCTCCTCTATGACCCAGACCACGCTGTCGGTGGa                                                                                                                                                   <  2:258171/288‑1 (MQ=255)                                                                                                                                       tcatgCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAtg                                                                                                                                     <  2:594754/295‑1 (MQ=255)                                                                                                                                              gTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTNACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGAc                                                                                                                             >  1:512145/1‑300 (MQ=255)                                                                                                                                              gTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTAAGATGGGACAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAACCGAAGAGAAGCTCTCCTTTATGACCCAGCCCACGCTGTCGGTGGATGACACGTCTGATGTGATCGa                                                                                                                              >  2:112184/1‑299 (MQ=255)                                                                                                                                              gTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCAACTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTAGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGCAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTAGGTGGATGACACGTCTGATGTGGTCGAc                                                                                                                             >  2:395252/1‑300 (MQ=255)                                                                                                                                                            aaaaaGCCGCGATGTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCAGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACg                                                                                                               <  1:372072/300‑1 (MQ=255)                                                                                                                                                                       aTTTGACGGTGTTTGCTGCCACATGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGACCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGGTCGACGCGCTGCGTAAACGCTTCCCGaaaa                                                                                                    >  2:135772/1‑300 (MQ=255)                                                                                                                                                                       aTTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCAGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGATTCCCGaaaa                                                                                                    >  2:366507/1‑300 (MQ=255)                                                                                                                                                                       aTTTGACGGTGTTTGATGCCACATGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAGGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGaaaa                                                                                                    >  2:135834/1‑300 (MQ=255)                                                                                                                                                                            aCGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGAccc                                                                                                                                                                      <  1:453334/229‑1 (MQ=255)                                                                                                                                                                            aCGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGAccc                                                                                                                                                                      >  2:453334/1‑229 (MQ=255)                                                                                                                                                                               gtgtTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATNACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGt                                                                                            >  1:496782/1‑300 (MQ=255)                                                                                                                                                                               gtgtTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGt                                                                                            >  1:461852/1‑300 (MQ=255)                                                                                                                                                                                tgtTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGTTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTc                                                                                           <  2:663230/300‑1 (MQ=255)                                                                                                                                                                                 gtTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCAGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGACAATTGTCGGTcc                                                                                          >  1:531202/1‑300 (MQ=255)                                                                                                                                                                                      aTGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCGGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGTATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACACAGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCATTTATGACCCAGACCACGCTGTCGGTGGATGAAACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTAACGAAAATTGTCGATCCGCGCa                                                                                     >  2:336247/1‑300 (MQ=255)                                                                                                                                                                                      aTGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCACGAAAATTGTCGGTACGCGCa                                                                                     >  2:336206/1‑300 (MQ=255)                                                                                                                                                                                                               aatGCATATGGAAGTCGCCCGCGCCAGCCGCCTTTGCGAAGAGTCGATTCTCATCGTCCACGCCGTGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAATGGGGAATGTATCTGTTCGAATCGCCGGACGATGTGTGTAAACTGACGGTCAAAAACGAATAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGAc                                                            <  2:238216/298‑1 (MQ=255)                                                                                                                                                                                                                  gCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTaa                                                         >  1:232014/1‑300 (MQ=255)                                                                                                                                                                                                                  gCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCACGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTaa                                                         >  1:443769/1‑300 (MQ=255)                                                                                                                                                                                                                    atatGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGCTTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCAGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTCTGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGAGCTGCGTAAACGCTTCAAGAAAATTGTCGGTCCGCGCAAAGATGCAATCTGCTAAGCCACGACTAAcc                                                       >  2:166204/1‑300 (MQ=255)                                                                                                                                                                                                                    atatGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAAcc                                                       <  1:669465/300‑1 (MQ=255)                                                                                                                                                                                                                    agatGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAATAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAAcc                                                       <  1:669443/298‑1 (MQ=255)                                                                                                                                                                                                                                   gcgcCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGt                                          <  1:194353/298‑1 (MQ=255)                                                                                                                                                                                                                                               gTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAg                            <  1:21893/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gaagagTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACa                        <  1:64131/299‑1 (MQ=255)                                                                                                                                                                                                                                                                 tcATCGGCCACGCCGGGCATCAGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGTGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTtgtg           <  2:47923/299‑1 (MQ=255)                                                                                                                                                                                                                                                                      ggccACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTtggt      >  1:293717/4‑299 (MQ=255)                                                                                                                                                                                                                                                                          aCGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTggtggt   <  1:623722/298‑1 (MQ=255)                                                                                                                                                                                                                                                                           cGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTc  >  1:540597/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                               ATATATGTCCGTCACGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTC  >  NC_000913/26373‑26936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑