Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,677	G→A	L157L (TTG→TTA)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,677	0	G	A	98.6%	226.0 / NA	70	L157L (TTG→TTA)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/46);  minor base C (1/0);  total (24/46)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGACTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCTGGGTGATGCTTCTCTTTCTGAA  >  NC_000913/25389‑25963                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                 tGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGc                                                                                                                                                                                                                                                                                       <  1:533818/300‑1 (MQ=255)                 tGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAAtaataa                                                                                                                                                                                                                                                                       >  1:428203/1‑300 (MQ=255)                   gCAGCGTTGTTTCTTTGCTGGTATTGTGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGAGCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCTGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCGGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAAc                                                                                                                                                                                                                                                                     <  2:48882/300‑1 (MQ=255)                      gCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCAGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAACACAATAATCAAccc                                                                                                                                                                                                                                                                   >  2:105636/1‑299 (MQ=255)                       cGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTACACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCCTCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTCACCTTCTACAGCGAAAAAACAATAATAAACCCt                                                                                                                                                                                                                                                                  >  2:382204/1‑299 (MQ=255)                       cGTTGGTTCTTTGCAGGTATTGCGGTTGGTATTAGCGTGATCCTGGCAGTGGTGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCATGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ATTTCGCAACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAAccat                                                                                                                                                                                                                                                                  >  2:382037/1‑297 (MQ=255)                          tGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTTGCAGGGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTGACGCGCTGATTATTGGCTGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCg                                                                                                                                                                                                                                                              <  2:407457/300‑1 (MQ=255)                                       gTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTNCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGa                                                                                                                                                                                                                                                 >  1:498604/1‑300 (MQ=255)                                              gATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGNCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATANTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCt                                                                                                                                                                                                                                          <  2:529753/300‑1 (MQ=255)                                                   gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGAACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTAGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGTGGTGCTGACGCATCTTATcc                                                                                                                                                                                                                                     >  2:2274/1‑300 (MQ=255)                                                   gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATACCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGTAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATcc                                                                                                                                                                                                                                     <  2:344199/300‑1 (MQ=255)                                                            tGATCCTGGTAGGGATGGTGTATCGCTAGAAGGCCACGCAGAAGCTAAACAATAGCGCTTACGCGCTGATTATTGGCGGTGCGCGGGGCAATCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGAATTCTACTTCGGCGGCTGAC‑ACTTCGCCACCTTCAACCTTGCCGATACGGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATTCGATGCTGACGCATCTTATCCGGCCTACAg                                                                                                                                                                                                                            <  2:126166/300‑1 (MQ=255)                                                            tGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTATACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAg                                                                                                                                                                                                                            <  1:601065/300‑1 (MQ=255)                                                               tCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGAtt                                                                                                                                                                                                                         <  1:29202/300‑1 (MQ=255)                                                               tCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTAGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGAtt                                                                                                                                                                                                                         >  1:60447/1‑300 (MQ=255)                                                                  tGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATtgct                                                                                                                                                                                                                      >  1:599285/1‑300 (MQ=255)                                                                  tGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTAGACCGCC‑TGTGGCACTGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGACGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATtgc                                                                                                                                                                                                                       <  1:16311/299‑1 (MQ=255)                                                                       gTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGaa                                                                                                                                                                                                                 >  1:573980/1‑300 (MQ=255)                                                                               gTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTa                                                                                                                                                                                                           >  1:210594/1‑298 (MQ=255)                                                                                        gAAGTCCACGCAGAAGCTGAATGATATCGCTGACGCGCTGATTGTTGGCGGCGCGCGGGTCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGATTTCTACGTCGGCGGCTGAC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCAGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcgattc                                                                                                                                                                                                                                                    <  2:277858/248‑3 (MQ=255)                                                                                        gAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATaa                                                                                                                                                                                               <  1:290558/299‑1 (MQ=255)                                                                                          aGGCCACGCATAAGCTAAACAAGACCGCTGACGCGCTGATTATGGGCGGCGCGCTGGGCAACCTGTTCTACCGCC‑TGTGGCACGGCTTCGTGGT‑CGATATGATCGACTTCTACTTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGGTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATTTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGc                                                                                                                                                                                            <  2:332734/300‑1 (MQ=255)                                                                                           ggCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTTGCGGCGCGCTGTGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTACCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCAGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCg                                                                                                                                                                                           <  1:228907/300‑1 (MQ=255)                                                                                                 gCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTAc                                                                                                                                                                                      <  2:631874/299‑1 (MQ=255)                                                                                                 ctgcgagatgtgtatangagacagcgcgCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGNTTGCTGCGAAATCGTAGGN‑‑CGGATAAGGCGTTTAc                                                                                                                                                                                      >  1:584643/25‑299 (MQ=255)                                                                                                   aGAAGCTAAACAATATCGCTTACGCGCTGGTTATTGGCGGCGCGCTGGGCAACCTGTTCGACCACC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTCCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTAcgc                                                                                                                                                                                    <  2:79345/299‑1 (MQ=255)                                                                                                                                            gctcggggCACCCCGTTCGACCACC‑TTTGGCACGGTTTCGT‑GTGCGATATGATCGACTTCTACTTCGGCGGCTGGC‑ACTTCGCCAACTTAAATCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGGGTGTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTGCAGATTGCGGCGAAATCGGAGGC‑‑CGGATAAGGCGTTTACGCAGCATCCGGCAAAAATCCTTAAATATAAAAGCAAACCTGCAt                                                                                                                                          <  2:69936/295‑1 (MQ=255)                                                                                                                                            gcgcTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTAGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGACACCTTCAAACTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTCCCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCa                                                                                                                                           >  2:94327/1‑299 (MQ=255)                                                                                                                                                 gggCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCt                                                                                                                                      >  1:623403/1‑299 (MQ=255)                                                                                                                                                 gggCAACCTGTTCGACCACC‑TGTGGTACGGCTTCGTGGT‑CGATATGAGCGACTTCTACGTCGGCGGCTGGCCACTTCGCCACCTTCAACCTTGCCGATACTGCCAGTTGGGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGAGGCTGACGCACCTTAGCCGGCCTACAAACTGCTGCGAAACCGTGGGCCGCGGATAAGGCGTTTAc                                                                                                                                                                                      <  1:239748/254‑1 (MQ=255)                                                                                                                                                     aaCCCGTTCGGCCGCCCTGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAAt                                                                                                                                  <  1:240248/300‑1 (MQ=255)                                                                                                                                                          gTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAc                                                                                                                            <  2:477236/300‑1 (MQ=255)                                                                                                                                                          gTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAc                                                                                                                            <  1:685992/300‑1 (MQ=255)                                                                                                                                                               accacc‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGc                                                                                                                       <  2:419127/296‑1 (MQ=255)                                                                                                                                                                  gCC‑TGTGGCACTGCTTCGTTGT‑CGATATGATCGACTTCTACGTCTGCGGCTTGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATTCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGc                                                                                                                       <  2:179373/297‑1 (MQ=255)                                                                                                                                                                    c‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACCGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAACCCTGCATGTCTGCATCTGTACAGAGCAATa                                                                                                                   >  2:80811/1‑299 (MQ=255)                                                                                                                                                                    c‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCAACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATa                                                                                                                   >  2:80171/1‑299 (MQ=255)                                                                                                                                                                    c‑TGTGGCACGGCTTCGTTGT‑CGATATAATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑AGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTATGAATCTGTACAGAGCAATa                                                                                                                   >  2:80838/1‑299 (MQ=255)                                                                                                                                                                      tttgacatgtcTTCGTTGT‑CGATAGGATCGACGTCTACGGCGTCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACGGCCATCTGTGTCGGTGCTGCACTGATTGTGCTGGAAGGTTTGTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAGATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAc                                                                                                                            <  2:385559/279‑1 (MQ=255)                                                                                                                                                                      tgtgGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAg                                                                                                                  <  1:89895/299‑1 (MQ=255)                                                                                                                                                                      tgtgGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAg                                                                                                                  <  2:598422/299‑1 (MQ=255)                                                                                                                                                                      tgtgGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCGGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGANGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAg                                                                                                                  <  2:498604/299‑1 (MQ=255)                                                                                                                                                                        tgGCACTGCTTCGTGGT‑CGATATGATCGACGTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAgcgc                                                                                                               <  2:98030/300‑1 (MQ=255)                                                                                                                                                                               gCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCCTCCGGCAAAAATCCTTAAACATAAGAGCAAACCTACATATCCGAATCTGCACAGAGCAATAGCGCCGTCCt                                                                                                         >  2:286006/1‑299 (MQ=255)                                                                                                                                                                                  tCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTgggg                                                                                                     <  1:258236/300‑3 (MQ=255)                                                                                                                                                                                  tCGTTGT‑CGATATGATCGACTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTg                                                                                                     <  2:600797/300‑1 (MQ=255)                                                                                                                                                                                                     actgcTACGTCTGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGNTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAAc                                                                                     <  2:537491/294‑1 (MQ=255)                                                                                                                                                                                                     aCTTCTACGTCGGCGGCTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTc                                                                                   >  1:683451/1‑300 (MQ=255)                                                                                                                                                                                                                   ggcTGGC‑ACTTCGCCACCTTCAACCTTGCCAATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGcaccac                                                                     <  2:384841/298‑1 (MQ=255)                                                                                                                                                                                                                      tGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTCGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCAccgc                                                                  <  2:477900/300‑1 (MQ=255)                                                                                                                                                                                                                           aCTTCGCCACCTTCAACCTTGCCTATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAACCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTACATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAg                                                              <  2:157640/300‑1 (MQ=255)                                                                                                                                                                                                                             ttCGCCACCTTCAACCNTGCCGATACTGCCATCTGNGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCCAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGANGGCACCACCGCCGAGt                                                             <  1:592716/299‑1 (MQ=255)                                                                                                                                                                                                                                   aCCTTCAACCTTGCCGATACTGCCATCTGTGTCGTTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTAccc                                                       <  1:184307/299‑1 (MQ=255)                                                                                                                                                                                                                                   aCCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTAccc                                                       <  1:190136/299‑1 (MQ=255)                                                                                                                                                                                                                                   aCCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTAcc                                                        <  1:398178/298‑1 (MQ=255)                                                                                                                                                                                                                                       tCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGcaac                                                  <  2:428203/300‑1 (MQ=255)                                                                                                                                                                                                                                          aCCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCAGTACTGGTGCACTTCACGCTAAAACTCGAAGATGGCACCACCGCCGAGTCTAACCGAaacaac                                               >  2:144397/1‑300 (MQ=255)                                                                                                                                                                                                                                          aCCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGCTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCAACGCCGAGTCTAACCGCaacaac                                               >  2:144343/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAATGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAAcc                                       <  2:128072/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCTTCGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTAACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGACGCTGTTcc                            >  1:558494/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gtgtCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGc                          <  1:8022/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 ttCGGTGCGGCACTGATGGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAGAACAATAATAAACCCTGCCGTATGCGATGCTGACGCATCTTATCCTGCCTACAGATTGCTTCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTTACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCt                        <  2:83043/299‑1 (MQ=255)                                                                                                                                                                                                                                                                 gtCGGGGCGGCGCTGATTGTGCGGGAAGGGTTTTTACCGTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCCTATCCGGCCTACAGATTGCTGCTAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTATAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACTGGCGCTGTTCCGCCt                        <  2:74701/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   cGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCgact                        >  1:626880/1‑295 (MQ=255)                                                                                                                                                                                                                                                                     gTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCTGGGt                    <  1:673553/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          gCACTGATTGTGCTGGAAGGTTTTTTACCTTATAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCNGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCNGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTACTGGTGCATTTCACGCTAAAACTAGACGATGGCACCACCGCCGAGTCTAACCGCAACAACGGTAAACCGGCGCTGTTCCGCCTGGGTGATGc               >  2:517957/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              tGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCTGGGTGCTGCTtct           >  2:293596/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               gATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCTGGGTGATGCTtctc          <  1:608519/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       tGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCTGGGTGATGCTTCTCTTTCTGaa  <  2:584643/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       tGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCTGGGTGATGCTTCTCTTTCTGa   <  1:38215/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                 TGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCC‑TGTGGCACGGCTTCGTTGT‑CGATATGATCGACTTCTACGTCGGCGACTGGC‑ACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGC‑‑CGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCTGGGTGATGCTTCTCTTTCTGAA  >  NC_000913/25389‑25963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑