Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,011,802	C→T	P243S (CCG→TCG)	bioP →	biotin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,011,802	0	C	T	98.3%	206.4 / ‑3.9	60	P243S (CCG→TCG)	bioP	biotin transporter
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (21/38);  total (21/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACC  >  NC_000913/4011530‑4012097                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                        ccATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTa                                                                                                                                                                                                                                                                              >  1:22404/1‑300 (MQ=255)                     aaaCGCCTGATTGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTTGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCAc                                                                                                                                                                                                                                                          <  2:408192/300‑1 (MQ=255)                     aaaCGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCAc                                                                                                                                                                                                                                                          <  1:85102/300‑1 (MQ=255)                                                     gCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGAt                                                                                                                                                                                                                          >  2:382585/1‑300 (MQ=255)                                                     gCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCAGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGAt                                                                                                                                                                                                                          >  2:209571/1‑300 (MQ=255)                                                     gCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTATGGTGGCAGTGATTGCGTGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGAACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGATGGTGAt                                                                                                                                                                                                                          >  2:210052/1‑300 (MQ=255)                                                       aTAACGCCTTTGCTTGGTTCTATCTTGGCGCGTTTCTGTTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATc                                                                                                                                                                                                                         <  2:612082/299‑1 (MQ=255)                                                       aTAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGTAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGNTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATc                                                                                                                                                                                                                         <  2:186447/299‑1 (MQ=255)                                                            gCCTTTTCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGcc                                                                                                                                                                                                                   <  2:362688/300‑1 (MQ=255)                                                            gCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTTGCAGTGATTGCATGGTTCTTGCTGGGAAATGCACAGAAAATGCCACAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGcc                                                                                                                                                                                                                   <  2:611480/300‑1 (MQ=255)                                                            gCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGcc                                                                                                                                                                                                                   <  1:458466/300‑1 (MQ=255)                                                            gCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCAT‑‑TGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGcc                                                                                                                                                                                                                   <  1:104151/298‑1 (MQ=255)                                                                  gCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACtg                                                                                                                                                                                                             <  1:143955/300‑1 (MQ=255)                                                                    gTGGTTCTATCTTGGCGCGTTTCTGGTTGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCGGCAATTGTGCATTCTGGTGTGTCTTGGCGTGGTGGCTTCTGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACtg                                                                                                                                                                                                             <  1:458332/298‑1 (MQ=255)                                                                    gTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACtgt                                                                                                                                                                                                            <  1:376595/299‑1 (MQ=255)                                                                      ggTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTggg                                                                                                                                                                                                         <  1:342134/300‑1 (MQ=255)                                                                       gTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGt                                                                                                                                                                                                        >  2:396349/1‑300 (MQ=255)                                                                        ttCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTg                                                                                                                                                                                                       <  1:108156/300‑1 (MQ=255)                                                                             tCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGGGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCg                                                                                                                                                                                                  <  2:412677/300‑1 (MQ=255)                                                                                 ggCGCGTTTCTGGTGTCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCTGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAg                                                                                                                                                                                              <  2:424496/300‑1 (MQ=255)                                                                                  gcgcGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGTGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGt                                                                                                                                                                                             <  1:356649/300‑1 (MQ=255)                                                                                  gcgcGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGt                                                                                                                                                                                             <  1:356412/300‑1 (MQ=255)                                                                                    gcgcttCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGAGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTg                                                                                                                                                                                            <  1:257361/295‑1 (MQ=255)                                                                                    gcgTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTgg                                                                                                                                                                                           <  1:525819/300‑1 (MQ=255)                                                                                    gcgTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTg                                                                                                                                                                                            <  1:309187/299‑1 (MQ=255)                                                                                     cgTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTG‑CTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGt                                                                                                                                                                                         >  1:579999/1‑300 (MQ=255)                                                                                      gTTTCTGGTGGCAGTGANTGCATGGTTCTTGCTGGGNAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGNTGCATCGTAAGTGGGt                                                                                                                                                                                         <  1:511934/300‑1 (MQ=255)                                                                                       tttCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTAACTGTGGGTGCATCGTAAGTGGGTc                                                                                                                                                                                        >  2:388925/1‑300 (MQ=255)                                                                                                 cAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGct                                                                                                                                                                              >  2:611264/1‑300 (MQ=255)                                                                                                      aTTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTca                                                                                                                                                                         >  1:574762/1‑300 (MQ=255)                                                                                                         gCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACaa                                                                                                                                                                      >  2:19909/1‑300 (MQ=255)                                                                                                                        ggAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGc                                                                                                                                                       <  1:148688/300‑1 (MQ=255)                                                                                                                        ggAAATGAGCAGAAAATTCCGCAAACCACGCTGCAATGGGGCATTCTTGTTTTTCTTGGCTTGTTGGCTTCCTGGATTGGCTACTTTATTTGGAACTACGGCGCGACGCAGGGGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGc                                                                                                                                                       <  2:324658/300‑1 (MQ=255)                                                                                                                             tGCGCAGAAAATGCCGCAAACCACGCTGCAATGGTGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCg                                                                                                                                                  <  1:14226/300‑1 (MQ=255)                                                                                                                             tGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTANTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCg                                                                                                                                                  <  1:124269/300‑1 (MQ=255)                                                                                                                             tGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCg                                                                                                                                                  <  1:14225/300‑1 (MQ=255)                                                                                                                                   gAAAATGCCGCAAACCACGCGGCAATGGGGCATTCTGGTGTTTCTTGGTGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATNTGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGTGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTg                                                                                                                                            <  2:191735/300‑1 (MQ=255)                                                                                                                                              aaaCCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGc                                                                                                                                  >  1:391623/1‑299 (MQ=255)                                                                                                                                                      cTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTCTGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGTTGTCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCATAACTGTGGGTGCATCgc                                                                                                                                                                                                 >  2:317261/1‑227 (MQ=255)                                                                                                                                                      cTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCCGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTCAGTGGGTCGCTCCGCGCTCTTCAAAAATGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGACTAAAc                                                                                                                          >  2:316957/1‑299 (MQ=255)                                                                                                                                                        gCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCAAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCt                                                                                                                       >  2:276437/1‑300 (MQ=255)                                                                                                                                                                  aTTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAAAGCTGTGCATTATGAATAATATGCACTGGTC‑GGCAGGGCGGCTGGGAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTgcgcgc                                                                                                                                       <  1:334952/274‑1 (MQ=255)                                                                                                                                                                          gtTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACgc                                                                                                     <  2:98755/300‑1 (MQ=255)                                                                                                                                                                               ttGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTc                                                                                                <  1:611128/300‑1 (MQ=255)                                                                                                                                                                                tgtcGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTc                                                                                                <  2:364176/296‑1 (MQ=255)                                                                                                                                                                                                          aCTTTATGTGGAACTACGGCGCGACGCAGGTGGANGCCGGAACGCTGGGCATTATGAATAATATGCAN‑GTTTCGGCACGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTg                                                                                                                                                                                                       <  2:467164/170‑1 (MQ=255)                                                                                                                                                                                                          aCTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCACGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTg                                                                                                                                                                                                       >  1:467164/1‑170 (MQ=255)                                                                                                                                                                                                          aCTTTATGGGGAACTAAGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCg                                                                     <  2:324314/300‑1 (MQ=255)                                                                                                                                                                                                                  tgGAACTACGGCGCGACGCAGGTGGACGCCGGAATGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTACCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTACTCGAACTCACGCCCGGCGGCGTACAGTCGGCTCcaca                                                             >  2:166333/1‑300 (MQ=255)                                                                                                                                                                                                                       cTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCt                                                        >  1:192567/1‑300 (MQ=255)                                                                                                                                                                                                                       cTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTACGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGcat                                                        >  1:192937/1‑298 (MQ=255)                                                                                                                                                                                                                            gcgcGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGcc                                                   <  1:422321/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aCGCTGGGCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTTGCCAACGTTTATTACAGGCGCGCTGGTGATCCTTGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGAc                            <  1:484199/300‑1 (MQ=255)                                                                                                                                                                                                                                                        gggCATTATGAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCt                         >  1:522959/1‑298 (MQ=255)                                                                                                                                                                                                                                                                 gAATAATATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCACAGGGTTTTGGTCACCACCAGACCCTGGCGCTCaaaa              >  1:425211/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aataatATGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAAc             <  1:303468/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         tGCAC‑GTTTCTGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTctac      <  2:539451/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         tGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTctac      <  2:539458/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         tGCAC‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATNGCGCGCTGAGCGAATAAACGCTTCCGTAACTGNCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTctac      <  2:463832/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             c‑GTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTAcc  >  1:567796/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                        CCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCAC‑GTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACC  >  NC_000913/4011530‑4012097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑