Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,955,304	C→T	R609R (CGC→CGT)	ilvD →	dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,955,304	0	C	T	100.0%	202.4 / NA	58	R609R (CGC→CGT)	ilvD	dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/36);  total (22/36)

ATCACCGACGGTCGTTTCTCTGGTGGCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCACTGCTTCTGCGGGTAACCA  >  NC_000913/3955008‑3955599                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        aTCACCGACGGTCGTTTCTCTGGTGGCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGAt                                                                                                                                                                                                                                                                                                      <  2:350277/300‑1 (MQ=255) aTCACCGACGGTCGTTTCTCTGGTGGCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGAAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGAt                                                                                                                                                                                                                                                                                                      <  1:376501/300‑1 (MQ=255)                      ggtggCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTtaa                                                                                                                                                                                                                                                                                 >  1:496714/1‑300 (MQ=255)                         ggCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTtaataa                                                                                                                                                                                                                                                                              <  1:564061/300‑1 (MQ=255)                          gCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTtaataa                                                                                                                                                                                                                                                                              <  1:565274/299‑1 (MQ=255)                          gCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTaataat                                                                                                                                                                                                                                                                             <  1:216170/300‑1 (MQ=255)                             ccTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACGGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGc                                                                                                                                                                                                                                                                          >  2:421252/1‑300 (MQ=255)                               tctGGTCTTTCCATCGTCCACGTCTCACCGGAAGCTGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCAGTCAGTTACAGGTAAGCGAGGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAACCTGTACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGc                                                                                                                                                                                                                                                                          <  2:328173/298‑1 (MQ=255)                               tctGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGTTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGc                                                                                                                                                                                                                                                                          <  2:327079/298‑1 (MQ=255)                                           aTCGGCCACGTCTCACCGTAAGCGGCAAGCGGCGGCAGCAGTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATGCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCTTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAAcccc                                                                                                                                                                                                                                                            <  2:504523/300‑1 (MQ=255)                                                  aCGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCgg                                                                                                                                                                                                                                                     <  1:9127/300‑1 (MQ=255)                                                            gtaagcggcaagcggcGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTTTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGaa                                                                                                                                                                                                                                           <  2:65833/298‑1 (MQ=255)                                                            ggAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGaa                                                                                                                                                                                                                                           <  1:399857/300‑1 (MQ=255)                                                            ggAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATNCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGaa                                                                                                                                                                                                                                           <  1:132987/300‑1 (MQ=255)                                                                                gcaTTGTCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCTTTGCATTCAGTTACAGGTAAGCGATGCCAAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGc                                                                                                                                                                                                                       <  2:106217/300‑1 (MQ=255)                                                                                gcaTTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGc                                                                                                                                                                                                                       >  1:52213/1‑300 (MQ=255)                                                                                      gCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATTGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGtgc                                                                                                                                                                                                                  <  1:285154/299‑1 (MQ=255)                                                                                          gggggggggtggtggcatGATTGCTATCGACATCCAGAAACGTGGCATTCGGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCACCGAACAAGGCGCGGTGCGTGATAAATCAAAGCTGGGGGGTTACTAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTACCGCATATTTAAGAGCa                                                                                                                                                                                                                      >  2:387227/18‑291 (MQ=255)                                                                                          gagtGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCTTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgc                                                                                                                                                                                                             <  2:421375/297‑1 (MQ=255)                                                                                          gATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCTGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgc                                                                                                                                                                                                             <  1:146777/300‑1 (MQ=255)                                                                                          gATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgc                                                                                                                                                                                                             <  1:502188/300‑1 (MQ=255)                                                                                          gATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCGCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTgcgc                                                                                                                                                                                                             <  2:100460/300‑1 (MQ=255)                                                                                                                gCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGgcgc                                                                                                                                                                                       >  2:424282/1‑300 (MQ=255)                                                                                                                         aTCCAGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAcgc                                                                                                                                                                              >  2:172157/1‑300 (MQ=255)                                                                                                                          tCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAcgcc                                                                                                                                                                             <  1:252819/300‑1 (MQ=255)                                                                                                                                           cAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGaaa                                                                                                                                                            >  1:41111/1‑300 (MQ=255)                                                                                                                                                 cAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCAGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGaaaaa                                                                                                                                                          >  2:350612/1‑296 (MQ=255)                                                                                                                                                                    tGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGTTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGt                                                                                                                                   <  1:364713/300‑1 (MQ=255)                                                                                                                                                                    tGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGt                                                                                                                                   <  1:364720/300‑1 (MQ=255)                                                                                                                                                                             gtcgtGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCAGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCATGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTAGCGTCTTGATAACGTCATTCTGGt                                                                                                                          >  2:16087/1‑300 (MQ=255)                                                                                                                                                                                         aGGACACTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCAGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACTAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTc                                                                                                                              <  2:169843/284‑1 (MQ=255)                                                                                                                                                                                                       gACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGCAGATCGCCAGCCAGTGc                                                                                                >  2:67404/1‑300 (MQ=255)                                                                                                                                                                                                          aaaGCCTGGACGCCGAAAAATCGTGAACATCAGGTCTCCTCTGCCCTGCGTGCTTATGCCAGCCTTGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATTGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGcac                                                                                              <  2:231958/299‑1 (MQ=255)                                                                                                                                                                                                            aGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTCAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCAGCTACAAAAAATGGAAAAACTGTCGTCGCGTCCTGATAACGTCCTTc                                                                                                                              >  2:204448/1‑265 (MQ=255)                                                                                                                                                                                                            aGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGc                                                                                           >  1:598434/1‑300 (MQ=255)                                                                                                                                                                                                                   aCGCCGAAAACTCTTGAACGTCAGGTCTCCTTTGCTCTGCGTGCTTATGCCAGCCTG‑CAACCAGCTCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTTGTGGGTTAATAATGGCTTACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCTACTATTTAAGATCAGTGCGGAGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTGTAAGc                                                                                    <  2:308436/299‑1 (MQ=255)                                                                                                                                                                                                                   aCGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCTGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCACGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGc                                                                                    <  2:393602/300‑1 (MQ=255)                                                                                                                                                                                                                   aCGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGc                                                                                    <  1:155674/300‑1 (MQ=255)                                                                                                                                                                                                                                  gAACGTCAGTTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACg                                                                     <  2:286395/300‑1 (MQ=255)                                                                                                                                                                                                                                  gAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAATGCGCGGTGCGTGATAAATCGAAGCGGGGGGGGTAATAAGTGCTGACTCGCAACCCCTGTCCGGTTCTCCGGAAGGTGCCGAATATTGAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGTTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCTGCGCATACg                                                                     <  2:286385/300‑1 (MQ=255)                                                                                                                                                                                                                                         atgTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACTAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGTTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCatgat                                                              <  2:335280/298‑1 (MQ=255)                                                                                                                                                                                                                                           gTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATGAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTNACGAGGCGGCGCAGGTTACGCCGCTACAAAAANTGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTTAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATgg                                                            <  2:466371/300‑1 (MQ=255)                                                                                                                                                                                                                                             gtcCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTAGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTACTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTAAGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCAAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCAATGATGGc                                                           >  2:533651/2‑299 (MQ=255)                                                                                                                                                                                                                                             ctcCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAAGCCCTGTCCGGTGCTCCGGAAGGTGCAGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGc                                                           >  2:533681/1‑299 (MQ=255)                                                                                                                                                                                                                                               ccTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAGTCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGACGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGCTAACGTCATCCTGGTGAAGCGCGaa                                                                                                                >  2:339736/1‑244 (MQ=255)                                                                                                                                                                                                                                               ccTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGc                                                           >  2:353439/1‑297 (MQ=255)                                                                                                                                                                                                                                               ccTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTAAAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATATGCCATGATGGc                                                           >  2:551333/1‑297 (MQ=255)                                                                                                                                                                                                                                                             gCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGNAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAAc                                          >  1:431436/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   gCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAGCTGGGGGGTTAATAATTGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATGCGCCATGATGGCGGGCCTGACGGAAGAACAGAAAg                                    <  2:159888/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              aaCCAGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGt                          >  2:154154/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                  aGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCTTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGAt                       <  1:178790/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aGCGCCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGAt                       <  1:486927/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gcgcCGACAAAGGCGCGGTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCNCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCAc                    <  1:135005/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               gcgcGGTGCGTGATAAATCGAAACTGGTGGGTTAATAATGGCTGACGCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCAc                    <  2:45450/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    gTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCACTGCTTCTGCGGGTAAcc   >  2:569272/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    gTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAACCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCACTGCTTCTGCGGGTAAcc   >  2:584807/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    gTGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGAGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCTGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGAAGGAAGAACAGAAAGCGCACGGCGTGATCAATGCTTCTGCGGGTAAcc   >  2:177713/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     tGCGTGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCACTGCTTCTGCGGGTAACCa  <  1:350612/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        ATCACCGACGGTCGTTTCTCTGGTGGCACCTCTGGTCTTTCCATCGGCCACGTCTCACCGGAAGCGGCAAGCGGCGGCAGCATTGGCCTGATTGAAGATGGTGACCTGATCGCTATCGACATCCCGAACCGTGGCATTCAGTTACAGGTAAGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGACGCTCGAGGTGACAAAGCCTGGACGCCGAAAAATCGTGAACGTCAGGTCTCCTTTGCCCTGCGTGCTTATGCCAGCCTGGCAACCAGCGCCGACAAAGGCGCGGTGCGCGATAAATCGAAACTGGGGGGTTAATAATGGCTGACTCGCAACCCCTGTCCGGTGCTCCGGAAGGTGCCGAATATTTAAGAGCAGTGCTGCGCGCGCCGGTTTACGAGGCGGCGCAGGTTACGCCGCTACAAAAAATGGAAAAACTGTCGTCGCGTCTTGATAACGTCATTCTGGTGAAGCGCGAAGATCGCCAGCCAGTGCACAGCTTTAAGCTGCGCGGCGCATACGCCATGATGGCGGGCCTGACGGAAGAACAGAAAGCGCACGGCGTGATCACTGCTTCTGCGGGTAACCA  >  NC_000913/3955008‑3955599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑