Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,912,074	G→A	S532F (TCC→TTC)	glmS ←	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,912,074	0	G	A	100.0%	207.2 / NA	63	S532F (TCC→TTC)	glmS	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/31);  total (32/31)

ATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCTCAATACGGCTCGGCAGCGCCTGCAGAC  >  NC_000913/3911786‑3912359                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                              aTTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGtttt                                                                                                                                                                                                                                                                                    >  1:353246/1‑300 (MQ=255)      aaaCATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTA‑AAAACCCGCATCCTGCTCGGCGAAGACATACAACTGACTGCCACGCGTGCGAACTTCTTAAATGTTGAATTTCCGTTTTTCCAGc                                                                                                                                                                                                                                                                              >  2:402504/1‑300 (MQ=255)                 gaagaaAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCgttgt                                                                                                                                                                                                                                                                    >  1:598042/1‑300 (MQ=255)                   agaaAAATGCCCCGCTTACGCAGTGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCTGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTc                                                                                                                                                                                                                                                                  <  1:284610/300‑1 (MQ=255)                   agaaAAATGCCCCGCTTACGCAGGGCATCCATTTATTATTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGTCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTc                                                                                                                                                                                                                                                                  <  2:222255/300‑1 (MQ=255)                   agaaAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTc                                                                                                                                                                                                                                                                  <  1:283901/300‑1 (MQ=255)                   agaaAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGGCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATTTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTc                                                                                                                                                                                                                                                                  <  2:222162/300‑1 (MQ=255)                               cGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaacaaca                                                                                                                                                                                                                                                      >  1:49221/1‑300 (MQ=255)                                gCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTTCAATCACCTCGTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCAAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaacaa                                                                                                                                                                                                                                                        <  2:174015/297‑1 (MQ=255)                                gCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaacaac                                                                                                                                                                                                                                                       <  1:539970/298‑1 (MQ=255)                                  ttACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATa                                                                                                                                                                                                                                                   <  1:178963/300‑1 (MQ=255)                                                 aTTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCTGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGc                                                                                                                                                                                                                                     <  1:402100/299‑1 (MQ=255)                                                       taCTCAACCTTAACCGATTTTGCCATGTTACGCGACCGTTCAACGTCGGTGCCTTTGATCAGCGCGACAGGCTAAGCCNGCAGATGCAGCGGAACGGTGTAGAAGCTCGGTGCAATCACCTCGTCCACATTCTGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGGTCGGCGAAGACATACAACGGGCCGCCACGCGCGCGAACTTCTTCAATGTGGAATTGCATTTTTTCCAGCAATTCTTTGTTCGGTGCAACAACAATAATTGGCATATCGGCATCAAtt                                                                                                                                                                                                                              <  1:135634/300‑1 (MQ=255)                                                        aCTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTAGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTAAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTAGGTGCAACAACAATCACCGGCATATCGGCATCAATTa                                                                                                                                                                                                                             >  2:175879/1‑300 (MQ=255)                                                            aaCCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCTGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAgcg                                                                                                                                                                                                                          <  2:347184/299‑1 (MQ=255)                                                            aaCCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAgcg                                                                                                                                                                                                                          <  1:483667/299‑1 (MQ=255)                                                             aCCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGcc                                                                                                                                                                                                                        >  1:576259/1‑300 (MQ=255)                                                                      gATTTTGCCAGGTTACGCGGCTGTTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGAc                                                                                                                                                                                                                 <  2:78813/298‑1 (MQ=255)                                                                               aGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCTGCATCAATTAGCGCCAGCGGACCGTGTTTCAGt                                                                                                                                                                                                      >  1:186309/1‑300 (MQ=255)                                                                                         gCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGNAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCcagcag                                                                                                                                                                                            <  1:434008/300‑1 (MQ=255)                                                                                         gCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCcagcag                                                                                                                                                                                            <  1:550763/300‑1 (MQ=255)                                                                                            ggTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAAAAACAATAAACGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGt                                                                                                                                                                                         >  1:294295/1‑300 (MQ=255)                                                                                                aaCGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCTGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGc                                                                                                                                                                                     <  1:384102/300‑1 (MQ=255)                                                                                                          ccTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTAGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGa                                                                                                                                                                           >  1:609621/1‑300 (MQ=255)                                                                                                                 tCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAag                                                                                                                                                                    <  1:315879/300‑1 (MQ=255)                                                                                                                         aCATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATctct                                                                                                                                                            >  1:406140/1‑300 (MQ=255)                                                                                                                         aCATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTAAGCGTGAATGTAAGAGATctct                                                                                                                                                            >  1:72341/1‑300 (MQ=255)                                                                                                                         aCATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTATTCCACATGCGGCATCTAGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATACCGGCATCAATTAGCGACAGTGGACCGTGTTTAAGTTCGCCAGCAGCGTAGGCTTAAGCGTGAATGCAAGAGATctct                                                                                                                                                            >  1:71492/1‑300 (MQ=255)                                                                                                                                  gCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTc                                                                                                                                                   >  1:407182/1‑300 (MQ=255)                                                                                                                                       cagcTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATgc                                                                                                                                              >  1:606096/1‑300 (MQ=255)                                                                                                                                                   aaCGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAgcgc                                                                                                                                  <  1:585320/300‑1 (MQ=255)                                                                                                                                                   aaCGGTGTAGAAGACCGGTGCAATCACCCCTCCCACATGTGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATgcgc                                                                                                                                            <  2:58562/290‑1 (MQ=255)                                                                                                                                                       gtgtagacccccgttgccgtCACCTCCTCCACATGCGGCATCTAGATGATGTGTATGTTATCGCTACTTACAAAACCCGCATCCTGATTGGCGAAGACATACAACTGACCGCCAAGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACGACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTAAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCGTACAGCGCGAtt                                                                                                                              >  2:288360/20‑300 (MQ=255)                                                                                                                                                       gtgtAGGAGATCGGTGCAATCACCTATTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGTGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAAACGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCCATGCGCCTTACAGCGCGAtt                                                                                                                              >  1:580809/1‑300 (MQ=255)                                                                                                                                                       gtgtAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGAtt                                                                                                                              >  1:988/1‑300 (MQ=255)                                                                                                                                                       gtgtAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCAAGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTATTTCACCTTCAATGCGCCTTCCAGCGCGAtt                                                                                                                              >  2:166839/1‑300 (MQ=255)                                                                                                                                                             aagaTCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTAc                                                                                                                        <  1:108956/300‑1 (MQ=255)                                                                                                                                                             aagaTCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTGATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTGCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTAc                                                                                                                        <  1:429792/300‑1 (MQ=255)                                                                                                                                                               gaTCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAACCCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAAACGGCATATCGGCATCAATTAGCGCCAACGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATTTCTTTCAACTTCCATGTGCCTTACAGTGCGATTGGGTACt                                                                                                                       >  2:318506/1‑299 (MQ=255)                                                                                                                                                                   ggTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCNGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATc                                                                                                                   <  1:116458/299‑1 (MQ=255)                                                                                                                                                                   ggTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATc                                                                                                                   <  1:540998/299‑1 (MQ=255)                                                                                                                                                                        aaTCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCAACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTATTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCa                                                                                                               >  1:417138/1‑298 (MQ=255)                                                                                                                                                                           cACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCATTCCAGCGCGATTGGGTACTGATCGCCAAGGcc                                                                                                          >  2:404733/1‑300 (MQ=255)                                                                                                                                                                                ctTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCACAGGa                                                                                                     >  2:566922/1‑300 (MQ=255)                                                                                                                                                                                 ttCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGaa                                                                                                    <  2:988/300‑1 (MQ=255)                                                                                                                                                                                              aTCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATg                                                                                        <  1:617015/299‑1 (MQ=255)                                                                                                                                                                                                       atgTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCaga                                                                               >  1:401384/1‑299 (MQ=255)                                                                                                                                                                                                         gtgCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGaa                                                                            >  1:419075/1‑300 (MQ=255)                                                                                                                                                                                                                                        acgagatgtgtataagagacagaCTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTcc                                             >  1:386165/23‑300 (MQ=255)                                                                                                                                                                                                                                                    aaGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCCGTTCGCCAGCCGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGGTGTTTGTCAGCGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATc                                 >  2:213321/1‑300 (MQ=255)                                                                                                                                                                                                                                                    aaGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGCTTGTCAGAGAAATCTTATGCCAGTGCTTCAATGCGTTTGTCCTGAGACAGCATc                                 >  2:513876/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCt                                >  1:369404/1‑299 (MQ=255)                                                                                                                                                                                                                                                       aCATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTTCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGc                              >  1:548817/1‑300 (MQ=255)                                                                                                                                                                                                                                                       aCATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACCGCATCTGc                              >  2:307936/1‑300 (MQ=255)                                                                                                                                                                                                                                                        cATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCt                             >  2:207720/1‑300 (MQ=255)                                                                                                                                                                                                                                                              aCTGACCGCCACGCGCGCGAACTTCTTCGATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTGTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCTCAATAc                       <  2:39436/300‑1 (MQ=255)                                                                                                                                                                                                                                                              aCTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCTCAATAc                       <  1:389645/300‑1 (MQ=255)                                                                                                                                                                                                                                                              aCTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTACAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGAATCAATTAGCGCCAGCGGACCGTGTTTAAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCAACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCTCAATAc                       >  2:606070/1‑300 (MQ=255)                                                                                                                                                                                                                                                                tGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCTCAATAc                       <  2:396590/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                    ttcttcAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCTCAATACGGCTCGGCAGCGCCTGCAGAc  <  2:295218/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                    ttcttcAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTNCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCTCAATACGGCTCGGCAGCGCCTGCAGAc  <  2:440714/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                    ttcttcAATGTTGAATTTCAGGTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCTTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCTCAATACGGCTCGGCAGCGCCTGCAGAc  <  2:49221/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                    ttcttcAATGTTGAAGTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCTCAATACGGCTCGGCAGCGCCTGCAGAc  <  2:82923/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                              ATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGTTTGTCCTGAGACAGCATCTGCTCAATACGGCTCGGCAGCGCCTGCAGAC  >  NC_000913/3911786‑3912359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑