Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,162	C→T	H255Y (CAC→TAC)	spoT →	bifunctional (p)ppGpp synthase/hydrolase SpoT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,162	0	C	T	100.0%	224.3 / NA	72	H255Y (CAC→TAC)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/51);  total (21/51)

GTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTAC  >  NC_000913/3822878‑3823448                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                               tggAAACTCTCGAAATTGATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTTAGTTTTGAGGCGCTGTATCCCAACCGTGATCGCGTAATCAAAGAAGTGGTGAAAGTCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGTCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATCTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGAc                                                                                                                                                                                                                                                                                  <  2:126123/297‑1 (MQ=255) gtgaaactccccaaggttATAGCCCGCGGGCGCACCGTTTAGGTATCCACCACATTAAAATCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGGAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCATAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGAc                                                                                                                                                                                                                                                                                  <  2:45258/283‑1 (MQ=255) gTGAAATTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTGGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAATAGATGATCCATAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGAc                                                                                                                                                                                                                                                                                  <  2:11278/299‑1 (MQ=255) gTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCTAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGAc                                                                                                                                                                                                                                                                                  <  1:220835/299‑1 (MQ=255)   gAAACTCTCGAGATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCANAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGTTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGAc                                                                                                                                                                                                                                                                                  <  2:444557/297‑1 (MQ=255)   gAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCACGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGAc                                                                                                                                                                                                                                                                                  <  1:220825/297‑1 (MQ=255)        tctcGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCNTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGc                                                                                                                                                                                                                                                                          <  2:437416/300‑1 (MQ=255)        tctcGAAATGTATAACCCACTGTCGCACCGTTTAGGTATCCACCGCATTAAAACCGAACTCTAAGAGCTGTGTTTTTAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCACGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGc                                                                                                                                                                                                                                                                          <  2:33929/300‑1 (MQ=255)                        ccGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTc                                                                                                                                                                                                                                                          >  2:554256/1‑300 (MQ=255)                                gcACCGTTTAGGTATCCACCACATGAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAATTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTc                                                                                                                                                                                                                                                  <  2:136661/300‑1 (MQ=255)                                   ccGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCACCCGTTATCGCGTAATCAAAGAAGTGGTGCAAGCCGCGCGCGGCACCCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCt                                                                                                                                                                                                                                                 >  1:335077/1‑298 (MQ=255)                                   ccGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAAACGTTATCGCATAATCAAAGAAGTGGTGAAAGCCGCGCGTGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGCCAGTGGTCGCGAGAAGCGCCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGGTCGTCAATGAtt                                                                                                                                                                                                                                                   >  2:41576/1‑296 (MQ=255)                                     gTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAAACGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGGTTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAACTGGTGCTCAAAAAGCAGCGTTTTTACTCGATCCTGGACATCTACGCTTTCCACGTGACCGCCAATGATTCTGaca                                                                                                                                                                                                                                             >  2:316684/1‑300 (MQ=255)                                     gTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAAACGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCAGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGaca                                                                                                                                                                                                                                             >  2:17084/1‑300 (MQ=255)                                                 accacATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATcgcg                                                                                                                                                                                                                                 <  1:287675/300‑1 (MQ=255)                                                    acaTTAAAACCAAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTAGCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCTTAAATAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGc                                                                                                                                                                                                                              <  2:303982/300‑1 (MQ=255)                                                                   tCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCTAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGcac                                                                                                                                                                                                                <  1:382067/299‑1 (MQ=255)                                                                     gAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGc                                                                                                                                                                                                             <  1:400953/300‑1 (MQ=255)                                                                      aaGAGCTGGGTTTTTAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTTAAAGCCGCGCGCGGCAACCGTAAATAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAg                                                                                                                                                                                                              <  2:288156/298‑1 (MQ=255)                                                                            tGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAATTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACa                                                                                                                                                                                                      <  1:290801/300‑1 (MQ=255)                                                                            tGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAATAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTAc                                                                                                                                                                                                       <  1:461763/299‑1 (MQ=255)                                                                            tGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAATAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACa                                                                                                                                                                                                      <  1:314481/300‑1 (MQ=255)                                                                            tGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACa                                                                                                                                                                                                      <  1:346322/300‑1 (MQ=255)                                                                             gggTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGAGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACa                                                                                                                                                                                                      <  1:293504/299‑1 (MQ=255)                                                                                         gcTGTATCCCAACCGTTATCGCGTAGTCGAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTCAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTTGATTTACTGCACAGTGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGCCATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACTTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTCCAAGCCTCGTcc                                                                                                                                                                                            >  2:607232/1‑297 (MQ=255)                                                                                         gcTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCCGGTGCACAGACTGTAc                                                                                                                                                                                                       >  2:607225/1‑286 (MQ=255)                                                                                         gcTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTcc                                                                                                                                                                                            >  1:240013/1‑297 (MQ=255)                                                                                           tGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAATAGATACGCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGATAAGCATCTTTATTCGATTTACTGCAAAATGGTGTTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCggtc                                                                                                                                                                                        <  1:191721/299‑3 (MQ=255)                                                                                           tGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGGGAAAGCCGCGCGCGGCAACCGTAAATAGATGATCCATAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACAGCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGc                                                                                                                                                                                        <  1:191592/299‑1 (MQ=255)                                                                                            gTATCCAAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGcc                                                                                                                                                                                       >  2:370471/1‑299 (MQ=255)                                                                                                       gTTATCGCTTAATCAAAGAAGTGTTGAAGGCCGCGAGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGANGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCTTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGAc                                                                                                                                                                            <  2:183521/299‑1 (MQ=255)                                                                                                        ttATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAATAGATGATCTAGAAGATTCCTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGAc                                                                                                                                                                            <  2:160488/298‑1 (MQ=255)                                                                                                         tATCGCGTAATCAAAGAATTGTTGAAAGCCGCGCGCGGCAACCGTAAATAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCTAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGGTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGAc                                                                                                                                                                            <  2:260010/297‑1 (MQ=255)                                                                                                         tATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGccctgtctcttatacacatctcgcagg                                                                                                                                                                         <  1:604849/300‑26 (MQ=255)                                                                                                                             ggtgAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCTAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGaa                                                                                                                                                     >  1:137580/1‑300 (MQ=255)                                                                                                                                                aaCCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGACGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGcac                                                                                                                                  <  2:60246/300‑1 (MQ=255)                                                                                                                                                aaCCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGcac                                                                                                                                  <  1:305478/300‑1 (MQ=255)                                                                                                                                                aaCCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGcac                                                                                                                                  <  2:499223/300‑1 (MQ=255)                                                                                                                                                 aCCGTCAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGAGCGTTTGC‑AGGGAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTNGATTTACTGCAAAATGGTGCTCAAAGAGCAGCNTTTTTAATCGATCATGGAAACCCACGCTTTCCGCGTGATCGTAAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCAGCGTGAAAGACTATATCGCAATTCCAAAAGCGAACGGCCATAAGTCGTTGcaca                                                                                                                                 >  2:453236/1‑300 (MQ=255)                                                                                                                                                 aCCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGcaca                                                                                                                                 >  1:392840/1‑300 (MQ=255)                                                                                                                                                     tAAATAGACGATCCAGAAGATTCTTTCTGAAATCGAAGGGCATTTGC‑AGGAAGCGGGAATACCGCGCCGCGTCAGGGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGTTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCGGTCTTTGCACAcc                                                                                                                               <  2:584127/298‑1 (MQ=255)                                                                                                                                                     tAAAGAGATGATCCAGAAGATTCTTTCTGAAAGCGAAGTGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCTAGAAGCATCTTTATTCGATCTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATTTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTATTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCACCATCCCAAAAGCGAACGGCTATCAGTCTTTGCACAcc                                                                                                                               <  2:584120/298‑1 (MQ=255)                                                                                                                                                        ggggatgatCCAGAAGATTCTTTCTGAAATCGAAGGGCGCTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGCGGTCGCGGGAAGCATCTTTATTCGATCTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGCCATCTACGCTTTCCGCGTGATCGTAAATGGCTCTAAAACCTGTTCTCGCGTGCTGAGCCAAATGCACAGACTGCACCAGCCGCGTCCGGGCCGCGTGAAAAACTATATCGCAATTCCAAAAGCGAACGGCCATCAGTCTCTACACACCTCgat                                                                                                                          >  2:280909/4‑300 (MQ=255)                                                                                                                                                          agaTGATCCAGAAGATTCTTTTTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatga                                                                                                                        <  2:583347/300‑1 (MQ=255)                                                                                                                                                          agaTGATCCAGAAGATTCTTTCTGAAATCGAGGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatga                                                                                                                        <  2:583278/300‑1 (MQ=255)                                                                                                                                                          agaTGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGNGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatga                                                                                                                        <  1:436132/300‑1 (MQ=255)                                                                                                                                                               atCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGc                                                                                                                   >  1:12097/1‑300 (MQ=255)                                                                                                                                                                      agaTTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCAc                                                                                                             >  1:550449/1‑299 (MQ=255)                                                                                                                                                                             ttctGAAATCTAAGGGCATTTGC‑AGGAAGCTGGAATACCGTGCCGCGTCAGTGGTCGCTATAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACNCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCACGTCCGGGTCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACTGCTATCAGTCTTTGCACACCTCGATGACCGGCCCGCACGGTGTGcc                                                                                                     <  2:184899/300‑1 (MQ=255)                                                                                                                                                                             ttctGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACNCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGTGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGcc                                                                                                     <  2:184059/300‑1 (MQ=255)                                                                                                                                                                                  aaagcGAAGGGCGTTTGN‑AGGAAGCGGGAATACCGTNCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCNCACGGTGTGCCGGTTg                                                                                                <  1:511256/296‑1 (MQ=255)                                                                                                                                                                                   atccGAAGGGCGTTTGCNAGGAAGCGGGAATACCGTNCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCTTGATCGTCAGTGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTTAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCNCACGGTGTGCCGGtgg                                                                                                <  1:510323/297‑3 (MQ=255)                                                                                                                                                                                       gAAGGGCGTTTGC‑AGGANGCGGGAATACCGTGCCGCNTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGNTGTGCCGGTTGAGGTc                                                                                           <  1:516796/300‑1 (MQ=255)                                                                                                                                                                                              gTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATc                                                                                     <  1:109198/299‑1 (MQ=255)                                                                                                                                                                                                     aGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAAGGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTGCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGTGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTAc                                                                                <  2:300651/298‑1 (MQ=255)                                                                                                                                                                                                       ggaGCGGGAATACTGTGCCGCGTCAGTGTTCGCTAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTTCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAATACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTAc                                                                                <  2:300641/294‑1 (MQ=255)                                                                                                                                                                                                           cGGGGATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGata                                                                        >  1:238827/1‑300 (MQ=255)                                                                                                                                                                                                                     gTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAACGTTTTTCCTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGTTATCAGTATTTGCACACCTAGATGATTGGCCCGCACGGTGTGCAGGTTGAGGTCCAGATCCGTACCGAAGATATGGAACAGAt                                                              >  1:167628/1‑300 (MQ=255)                                                                                                                                                                                                                          gcgTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTTAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCgg                                                         <  2:347263/300‑1 (MQ=255)                                                                                                                                                                                                                          gcgTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGc                                                           >  1:282049/1‑298 (MQ=255)                                                                                                                                                                                                                          gcgTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCgg                                                         <  2:346748/300‑1 (MQ=255)                                                                                                                                                                                                                                  ggTCGCGATAAGCATCTTTATTCGATTTACTGCAAAATGGTGCGCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgt                                                 <  2:145023/300‑1 (MQ=255)                                                                                                                                                                                                                                               aTCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCAc                                     <  1:40372/299‑1 (MQ=255)                                                                                                                                                                                                                                                   ttATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAGTGATTCTGACACCTGTTATCGCGTGCTGTGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGc                                <  2:92630/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATaa                          <  1:554256/300‑1 (MQ=255)                                                                                                                                                                                                                                                           tttACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATTGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAag                        <  2:515911/300‑1 (MQ=255)                                                                                                                                                                                                                                                               cTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGTTATCAGTCTTTGCACACCTAGATGATCGGCCCGCACGGCGTGCCGGTTGAGGTCCAGACCCGCACCGACGATATAGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATACaga                       >  2:293543/1‑297 (MQ=255)                                                                                                                                                                                                                                                                 gCAAAATGGTGCTCAAAGCACAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCCCGGTGTGCCGGTTGAGGTCCAGCTCCGTACCGAAGATATGGACCAGATGACGGAGATGGGTGTTGCCGCGCATTGGGCTTATAAAGAGCAc                   >  2:549973/1‑299 (MQ=255)                                                                                                                                                                                                                                                                   aaaaTGGTGCTCAAAGAGCAGCGTTTTTACTCGAGCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGGTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGc                <  2:53685/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           gCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCa         >  1:192755/1‑299 (MQ=255)                                                                                                                                                                                                                                                                               aaaGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGtac     <  1:597932/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 agagCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGtactac  <  1:95977/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                               GTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGC‑AGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTAC  >  NC_000913/3822878‑3823448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑