Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,543,877	G→A	P237P (CCG→CCA)	rpnA →	recombination‑promoting nuclease RpnA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,543,877	0	G	A	100.0%	204.6 / NA	56	P237P (CCG→CCA)	rpnA	recombination‑promoting nuclease RpnA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/33);  total (23/33)

ATCCCAAACTGGCAAGGCAGCTTTATGCCTCCGCATTTCCGCTGATTGATGTCACCGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCGAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCG‑‑GCCGGATG‑CGAAATAAATGGCGCATGG‑GCCGCTTTGGCGAAGATATATGA  >  NC_000913/3543591‑3544166                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                         aTCCCAAACTGGCAAGGCAGCTTTATGCCTCCGCATTTCCGCTGATTGATGTCACCGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGTCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGgag                                                                                                                                                                                                                                                                                            <  2:567476/299‑1 (MQ=255)           ggCAAGGCAGCTTTATGCCTCCGCATCTCCGCTGATTGATGTCACCGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATTGGGCTGGTAGAGCAAATGGCCTGCTTATTGAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATg                                                                                                                                                                                                                                                                                   <  2:145993/298‑1 (MQ=255)                                              ttgatGTCACCGTCATGCTTGGTGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATTGGGCTGGTAGAGCAAATGGCCTGCTTATTAGGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATATTGCAAACTGTCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATg                                                                                                                                                                                                                                                                                   <  2:146432/263‑1 (MQ=255)                                                      atctgcATGCCTGATGATGAAGTTGTGCAGCACCGACGTATGGCGCTGCTGGAGGTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGGGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGTGCTGTTTAATTACATACTGCAAACTGTCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCTGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGc                                                                                                                                                                                                                                       <  2:613553/294‑1 (MQ=255)                                                      accttCATGCCTGATGATGAAATCATGCAGCACCGACGTATTGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATNGTGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGc                                                                                                                                                                                                                                       <  2:483043/295‑1 (MQ=255)                                                      aCCGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATTGGGCTGTTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGc                                                                                                                                                                                                                                       <  2:77329/299‑1 (MQ=255)                                                      aCCGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATNGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGc                                                                                                                                                                                                                                       <  2:472265/299‑1 (MQ=255)                                                      aCCGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGc                                                                                                                                                                                                                                       <  1:565885/299‑1 (MQ=255)                                                      aCCGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGc                                                                                                                                                                                                                                       <  2:520716/299‑1 (MQ=255)                                                        cGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGTTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAGTTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTACTGACTATTGCGGAAAGCTTGCGGCGGAAGGGGGAACAATCCAAAGCCCt                                                                                                                                                                                                                                    >  2:418754/1‑300 (MQ=255)                                                        cGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGcact                                                                                                                                                                                                                                    >  2:418740/1‑297 (MQ=255)                                                            aTGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCat                                                                                                                                                                                                                                <  2:263936/300‑1 (MQ=255)                                                            aTGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCGGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCat                                                                                                                                                                                                                                <  1:461357/300‑1 (MQ=255)                                                                    tgatgaAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCaa                                                                                                                                                                                                                        >  2:371888/1‑300 (MQ=255)                                                                         aaaTCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAaataa                                                                                                                                                                                                                   >  1:144527/1‑300 (MQ=255)                                                                              aTGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCtt                                                                                                                                                                                                              >  1:384739/1‑300 (MQ=255)                                                                              aTGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCATGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCtt                                                                                                                                                                                                              >  1:384621/1‑300 (MQ=255)                                                                                        gACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGTAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGcctgtctcttatacacatctcgc                                                                                                                                                                                                     <  1:537739/299‑22 (MQ=255)                                                                                         aCGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGt                                                                                                                                                                                                   >  1:313285/1‑300 (MQ=255)                                                                                          cGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGtt                                                                                                                                                                                                  >  1:185475/1‑300 (MQ=255)                                                                                           gTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTc                                                                                                                                                                                                 >  1:603638/1‑300 (MQ=255)                                                                                                    tgctgGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAg                                                                                                                                                                                        <  1:247738/300‑1 (MQ=255)                                                                                                    tgctgGAGTGAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGTCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAg                                                                                                                                                                                        <  1:247658/300‑1 (MQ=255)                                                                                                        ggAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGAcat                                                                                                                                                                                    <  1:243278/300‑1 (MQ=255)                                                                                                              aaTTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAATGGCTGTTTAATTACATACTGCAAACTTGCGACGCTGTACGTTTTAACGATTGTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATgcg                                                                                                                                                                              <  2:75005/300‑1 (MQ=255)                                                                                                                    aaaaCATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTAc                                                                                                                                                                        <  1:210617/300‑1 (MQ=255)                                                                                                                                   aCGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCagaaga                                                                                                                                                         <  2:395774/300‑1 (MQ=255)                                                                                                                                       gACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTATTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAagag                                                                                                                                                     <  1:25762/300‑1 (MQ=255)                                                                                                                                       gACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGNTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAagag                                                                                                                                                     <  1:439086/300‑1 (MQ=255)                                                                                                                                            gATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGc                                                                                                                                                >  1:149140/1‑300 (MQ=255)                                                                                                                                                 ggCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTgc                                                                                                                                             >  1:421811/1‑298 (MQ=255)                                                                                                                                                     ggTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGGGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGa                                                                                                                                        >  1:342276/1‑299 (MQ=255)                                                                                                                                                                 gtccTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTc                                                                                                                          <  2:234137/298‑1 (MQ=255)                                                                                                                                                                 ggCCTGCTTATTAAGTAGTGTATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTc                                                                                                                          <  2:206262/300‑1 (MQ=255)                                                                                                                                                                     tGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGt                                                                                                                       <  1:30926/299‑1 (MQ=255)                                                                                                                                                                      gCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGAAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTATTGCAGACATCATGCGCTTTAACGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑Gtt                                                                                                                           >  2:225709/1‑294 (MQ=255)                                                                                                                                                                                          gCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCt                                                                                                 >  2:380112/1‑300 (MQ=255)                                                                                                                                                                                              atggcagacaAATCAAAGGGCTGTTTAATTACATACTGCAAACTGTCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGTGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTacac                                                                                             <  2:296812/296‑1 (MQ=255)                                                                                                                                                                                              aTGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTacac                                                                                             <  1:467928/300‑1 (MQ=255)                                                                                                                                                                                               tGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACAcc                                                                                            <  1:455562/300‑1 (MQ=255)                                                                                                                                                                                                     acaAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACAccc                                                                                           <  1:198444/295‑1 (MQ=255)                                                                                                                                                                                                       aaaTCAAAGGGCTGTTTAATTACATACTGCAAACTTGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCt                                                                                     <  1:188219/299‑1 (MQ=255)                                                                                                                                                                                                          tCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCaa                                                                                 <  1:2791/300‑1 (MQ=255)                                                                                                                                                                                                                  cTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTAGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGccacca                                                                         >  2:528952/1‑300 (MQ=255)                                                                                                                                                                                                                  cTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTATTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCTGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCAACCTACACCCTCTGCTTAAACGccacca                                                                         >  1:324909/1‑300 (MQ=255)                                                                                                                                                                                                                    gTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACcagc                                                                       >  1:501379/1‑300 (MQ=255)                                                                                                                                                                                                                       tAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACcagca                                                                      >  1:405456/1‑298 (MQ=255)                                                                                                                                                                                                                         aTTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGTCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTTCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGt                                                                   >  1:594118/1‑299 (MQ=255)                                                                                                                                                                                                                                 cTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTATTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGANTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCCCTGCTTAAACGCCACCAACAGGTGACaaaaat                                                          >  2:471166/1‑298 (MQ=255)                                                                                                                                                                                                                                                     aCGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCG‑‑GCCGGATG‑CGaaataaat                                   <  1:388333/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  tttATCGACGGCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCG‑‑GCCGGATG‑CGAAATAAATGGCGCATGG‑Gcc                      >  1:555053/1‑299 (MQ=255)                                                                                                                                                                                                                                                                     aTCGACGTCGTTGCCGAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCG‑‑GCCGGATG‑CGAAATAAATGGCGCATGG‑GCCGCtt                  <  2:258721/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               ttGCCGAACGTTCACCAAAGCA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCG‑‑GCCGGATG‑CGAAATAAATGGCGCATGG‑GCAGCTTTGGCGAAggt        >  2:365005/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                    gAACGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCG‑‑GCCGGATG‑CGAAATAAATGGCGCATGG‑GCCGCTTTGGCGAAGatatat    <  1:565329/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                     aaCGTTCACCAAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCG‑‑GCCGGATG‑CGAAATAAATGGCGCATGG‑GCCGCTTTGGCGAAGATATATGa  <  2:270331/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aCGTTCACCAAAACAGNCAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAACGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCACCGCCACCAGCAGGTGACAAAACTCGGCGCCGGATGGNGAAATAAATGGCGCATGGNGCCGCTTTGGCgcga          >  1:505435/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                         ATCCCAAACTGGCAAGGCAGCTTTATGCCTCCGCATTTCCGCTGATTGATGTCACCGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCGAAACA‑‑CAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAA‑GTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCG‑‑GCCGGATG‑CGAAATAAATGGCGCATGG‑GCCGCTTTGGCGAAGATATATGA  >  NC_000913/3543591‑3544166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑