Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,889,365	C→T	K245K (AAG→AAA)	cysI ←	sulfite reductase, hemoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,889,365	0	C	T	100.0%	165.6 / NA	51	K245K (AAG→AAA)	cysI	sulfite reductase, hemoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/30);  total (21/30)

CATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGC  >  NC_000913/2889088‑2889658                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      cATATGGACGGATCGGTTCAAATTTGCTCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCCAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCACACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATc                                                                                                                                                                                                                                                                                     >  2:355260/1‑296 (MQ=255) cATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCa                                                                                                                                                                                                                                                                                 >  2:394507/1‑300 (MQ=255) cATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCa                                                                                                                                                                                                                                                                                 >  1:53940/1‑300 (MQ=255) cATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTAAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCa                                                                                                                                                                                                                                                                                 >  2:75019/1‑300 (MQ=255)       gACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGt                                                                                                                                                                                                                                                                           <  2:397497/300‑1 (MQ=255)            aTCGGTTCAAATTTGATCCCCGCGCTACGCTCCACTTCCGCTTTAAACTTCTCAACCCCCACTCGCTCCAGCTTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTTAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCTGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCagg                                                                                                                                                                                                                                                                      <  2:609865/300‑3 (MQ=255)               ggTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTc                                                                                                                                                                                                                                                                    <  1:403284/299‑1 (MQ=255)                gTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGt                                                                                                                                                                                                                                                                  >  1:365083/1‑300 (MQ=255)                                   gcgACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTTTTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGatat                                                                                                                                                                                                                                                <  1:95984/299‑1 (MQ=255)                                           ccACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGtt                                                                                                                                                                                                                                            >  1:421466/1‑295 (MQ=255)                                                   gCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGNCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATc                                                                                                                                                                                                                               <  1:135134/300‑1 (MQ=255)                                                    cttcaaaCGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCAAGCGTATGCTCCAGCGGCAGATAGCAAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCCCCAACAGGTTACAGCCCACCAGTTTGCCGTTCTCGCCGATCGCCCCAAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTTTGTGGCGGGATCa                                                                                                                                                                                                                              >  2:44713/5‑300 (MQ=255)                                                        aaaCGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTAc                                                                                                                                                                                                                          <  1:32273/300‑1 (MQ=255)                                                                  aCCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTAACGTGGtttt                                                                                                                                                                                                                 >  1:586983/1‑299 (MQ=255)                                                                                 aGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTTAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCagg                                                                                                                                                                                                 <  1:140592/300‑1 (MQ=255)                                                                                 aGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCagg                                                                                                                                                                                                 <  1:454347/300‑1 (MQ=255)                                                                                     tgtATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTNCAGCGGCAGATATCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCaggtagg                                                                                                                                                                                             <  2:478757/300‑1 (MQ=255)                                                                                       tATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTc                                                                                                                                                                                           <  2:341737/300‑1 (MQ=255)                                                                                       tATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTc                                                                                                                                                                                           <  1:402348/300‑1 (MQ=255)                                                                                             gTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCg                                                                                                                                                                                     <  1:201652/300‑1 (MQ=255)                                                                                              ttttGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCTAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCg                                                                                                                                                                                     <  1:201636/299‑1 (MQ=255)                                                                                                 tGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGa                                                                                                                                                                                 <  2:53339/300‑1 (MQ=255)                                                                                                       tttgacgacctgttcgtttACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGGAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTTGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGa                                                                                                                                                                                 <  2:54915/283‑1 (MQ=255)                                                                                                                 ggttcggttACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCagt                                                                                                                                                                 <  2:522771/300‑1 (MQ=255)                                                                                                                 ggttcggttACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCag                                                                                                                                                                  <  2:397517/299‑1 (MQ=255)                                                                                                                   ttctgttgccccGGTCACACTGAGTTTTCACGACGGCTTCGTCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACCCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTGAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTTTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCagtag                                                                                                                                                               <  2:230018/292‑1 (MQ=255)                                                                                                                     cggttACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCagtagt                                                                                                                                                              >  1:373999/1‑299 (MQ=255)                                                                                                                           ccccAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACt                                                                                                                                                       >  1:558970/1‑300 (MQ=255)                                                                                                                                   aCGCTGAGTTGTCACTACTGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCTACTTTTTcc                                                                                                                                                 <  2:53940/298‑1 (MQ=255)                                                                                                                                    cGCTAAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCAAGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATCTACGCGGCAGGTAGGTCTGGCCGAGAATCGGTTCTTAATCAGTAGTGGCGACtttttact                                                                                                                                                >  1:216925/1‑295 (MQ=255)                                                                                                                                     gCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCACACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTAGCGACTTTTTCCTGGt                                                                                                                                             >  2:386900/1‑300 (MQ=255)                                                                                                                                                                      gCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGGCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAGGCCCACAAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGa                                                                                                             >  2:534569/1‑299 (MQ=255)                                                                                                                                                                        gTATGCTCCAGCGGCAGATATCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGTCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCTGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAgg                                                                                                           <  2:328493/299‑1 (MQ=255)                                                                                                                                                                             cTCCCGCGGCAGCTAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATTGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGAAACa                                                                                                      >  1:602703/1‑299 (MQ=255)                                                                                                                                                                                           aGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCTACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAAtct                                                                                       >  1:86195/1‑300 (MQ=255)                                                                                                                                                                                                            gTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTAc                                                                      >  1:218538/1‑300 (MQ=255)                                                                                                                                                                                                              gCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGTTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACTAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTNACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGc                                                                    <  2:439075/300‑1 (MQ=255)                                                                                                                                                                                                                 ggCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTc                                                                 >  1:9830/1‑300 (MQ=255)                                                                                                                                                                                                                                              tGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCTGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAggg                                        <  2:365083/296‑1 (MQ=255)                                                                                                                                                                                                                                                   aGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTTAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGt                                >  1:492649/1‑299 (MQ=255)                                                                                                                                                                                                                                                    gcccaccgcccaccAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGCGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGc                              >  1:486063/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gcccaccAACAGGTTAGAGCCCGCCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCTTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACTAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGt                         <  2:130986/298‑1 (MQ=255)                                                                                                                                                                                                                                                           gcccaccAACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGt                         <  1:75019/298‑1 (MQ=255)                                                                                                                                                                                                                                                               accaACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGtt                    <  1:355260/299‑1 (MQ=255)                                                                                                                                                                                                                                                               accaACAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCACCACGAAGTTCATGGCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGtt                    <  2:269415/299‑1 (MQ=255)                                                                                                                                                                                                                                                               acaaaCAGTTTAAGGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGGAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGTTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTTGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACTAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGtt                    <  2:267649/296‑1 (MQ=255)                                                                                                                                                                                                                                                                  aaCAGGTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTAc                  <  1:371698/298‑1 (MQ=255)                                                                                                                                                                                                                                                                      ggTTAAAGCCCACCAGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCa            >  1:497919/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      ggTTAAAGCCCAACGGTTTGCCGTTTTCAGCGATTGCCACGGAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTAAGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCAAGATCTACGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCGCCGCCAACTCGTAAGCTTCCGCGTGCAGCTGc                                                    >  2:308658/1‑260 (MQ=255)                                                                                                                                                                                                                                                                                 accaGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGTTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGc  <  1:497529/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 accaGTTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGc  <  1:497518/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      CATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGGCATTTTTACGATCGGTTCGGTTACCCCAGTCACGCTGAGTTGTCACGACGGCTTCGGCCACCGCCAGCGTATGCTCCAGCGGCAGATAGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGCGTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGATCGGTTCTTCATCAGTAGTGGCGACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGC  >  NC_000913/2889088‑2889658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑