Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,878,077	C→T	intergenic (+459/+492)	iap → / ← cas2	alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,878,077	0	C	T	100.0%	170.6 / NA	52	intergenic (+459/+492)	iap/cas2	alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/23);  total (29/23)

GGGAACTCTCGGTTCAGGCGTTGCAAACCTGGCTACCGGGCGGTTTATCCCCGCTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCCCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCT  >  NC_000913/2877783‑2878372                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        gggAACTCTCGGTTCCTGCGTTACCAACCTGGCTACAGGGCGGTTTATCCCCGCTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCACGCTGGCGCGGGGAACTCAAGTTGGTTGGCAATCTATTTAGGGGTGAGTCCGGTTTATCCTCGCt                                                                                                                                                                                                                                                                                                     >  2:262956/1‑299 (MQ=255)         tcGGTTCAGGCGTTGCAAACCTGGCTACCGGGCGGTTTATCCCCGCTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCgggg                                                                                                                                                                                                                                                                                            <  1:76235/300‑1 (MQ=255)                 ggCGTGGCAAACCTGGATACAGGGCGGTCTATCCCCGCTGACGCGGGGAACGCCTAGTCCATCATTCCACCTATGTCGGAACTCCCGGTTTATCCCCGCTGGCGCTGGGAACTCCCGGGGGATAACGTTTACGGCCATGCTCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACGCTGGGCGTCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAg                                                                                                                                                                                                                                                                                    <  2:511900/300‑1 (MQ=255)                                           gTTTATCCCCGCTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGc                                                                                                                                                                                                                                                          >  1:335485/1‑300 (MQ=255)                                                     gCTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGTGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGt                                                                                                                                                                                                                                                   <  2:415527/297‑1 (MQ=255)                                                     gCTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGt                                                                                                                                                                                                                                                   <  2:415346/297‑1 (MQ=255)                                                             ggggAATTCGTAGTCCATCATTCCACCTATGTCTGACCTTCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGTGGATAATGTGTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGTGGGGAACTCTGGGTGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCGGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCt                                                                                                                                                                                                                                        <  2:320417/300‑1 (MQ=255)                                                                  aCTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTCTCCCCGCTGgcgc                                                                                                                                                                                                                                   >  2:594921/1‑300 (MQ=255)                                                                                atTCCACCTATGTCTGAACTCCCGTTTTATCCCCGCTGGCGCGGGGAACTCCCGTGGGATAATGTTGACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGTGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCCCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGt                                                                                                                                                                                                                                                   <  2:415357/270‑1 (MQ=255)                                                                                        tATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGAc                                                                                                                                                                                                              <  1:237605/299‑1 (MQ=255)                                                                                           gTCTGAACTCCCGGTTTATCCCCGCTGGTGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCACGGTTTATCCCCGCTGGTGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCAGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTACGTATCTACGGATTTATAAAGATGAc                                                                                                                                                                                                                                                      >  2:277977/1‑256 (MQ=255)                                                                                           gTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGAAgcgc                                                                                                                                                                                                          >  1:339642/1‑300 (MQ=255)                                                                                                       ggTTTATCCCCGCTGGCGCGGGGAACTCCTGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTTGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATgcg                                                                                                                                                                                                <  2:115245/298‑1 (MQ=255)                                                                                                       ggTTTATCCCCGCTGGCGCGGGGAACTCCCGTGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCTTGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATgcg                                                                                                                                                                                                <  1:544448/298‑1 (MQ=255)                                                                                                        gTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGa                                                                                                                                                                                             >  1:361085/1‑300 (MQ=255)                                                                                                                    tGGCACGGGGAACTCCCGTGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCACTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATgcg                                                                                                                                                                                                <  2:32233/285‑1 (MQ=255)                                                                                                                                             aTGTTTGCGGTCGTGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGTCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCGGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATgcgc                                                                                                                                                                                               <  2:362763/261‑1 (MQ=255)                                                                                                                                              tGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCTTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCNGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGAc                                                                                                                                                       <  2:190631/300‑1 (MQ=255)                                                                                                                                              tGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGAc                                                                                                                                                       <  1:576758/300‑1 (MQ=255)                                                                                                                                                      gTCATGTGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTATTTCGGGGTGAGTCCGGTTTATCCTCGCTGGTGCGGGGAACTCTAGTTTCCGTATCTCCGGCTTTCCAAAGCTGACGGTTTATCCCCGCTAGCGCGGGGAACTCGCAGGCGGCGACGCGCGGGGTATGCGCGATTCGCGGTTTATCCCCGCTAGCGCGGGGAACTCGCAACCGTTCAGa                                                                                                                                               >  2:276347/1‑300 (MQ=255)                                                                                                                                                      gTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATACGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGa                                                                                                                                               >  2:333893/1‑300 (MQ=255)                                                                                                                                                              ccccccGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCa                                                                                                                                        >  1:394450/1‑299 (MQ=255)                                                                                                                                                                cccaggTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGAACGCTCAGAAATTCCAGAc                                                                                                                                     >  1:311455/5‑300 (MQ=255)                                                                                                                                                                      tttATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCGGGCGCGGGGAACTCAAGCTGGCGGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGAcc                                                                                                                                    <  2:74765/295‑1 (MQ=255)                                                                                                                                                                             ccGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCCGACGGCGACGCACAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGACGCGGGGAACTCGCGACCGCTCAAAAATTcccaaccagatc                                                                                                                               >  2:371096/1‑283 (MQ=255)                                                                                                                                                                                  ggCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATACCCGCTGGCGCGGGGAACTAGCGACAGCTCAGAAATTCCAGACCCGATCTAAACGGTTTa                                                                                                                    >  2:398790/1‑299 (MQ=255)                                                                                                                                                                                       ggggAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATcccc                                                                                                               >  2:357021/1‑299 (MQ=255)                                                                                                                                                                                          gAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCgat                                                                                                            >  1:183622/1‑297 (MQ=255)                                                                                                                                                                                          gAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCt                                                                                                            >  1:490516/1‑299 (MQ=255)                                                                                                                                                                                              tctGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGgcg                                                                                                        <  1:77633/299‑1 (MQ=255)                                                                                                                                                                                                   gctgcTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCgggg                                                                                                   <  1:96486/296‑1 (MQ=255)                                                                                                                                                                                                          gccttgcAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCACACGGTTTATCCCCGCTGGCGCGGGGAACTCTCa                                                                                           >  1:547397/1‑300 (MQ=255)                                                                                                                                                                                                            cttgcAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCAGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCaa                                                                                          >  2:385986/1‑299 (MQ=255)                                                                                                                                                                                                             ttgcAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACa                                                                                        <  1:387082/300‑1 (MQ=255)                                                                                                                                                                                                                                  gTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTACGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAATGGTTTATCCACGCTGGCGCGGGGAACTCTAAACATTATCAATTACAACCGACa                                                                     >  1:162280/1‑298 (MQ=255)                                                                                                                                                                                                                                          ccGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCTACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGccc                                                             >  1:399519/1‑298 (MQ=255)                                                                                                                                                                                                                                            gCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCgg                                                           <  1:425058/298‑1 (MQ=255)                                                                                                                                                                                                                                            gCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGtt                                                         <  1:357021/300‑1 (MQ=255)                                                                                                                                                                                                                                                  gctggggaCTCAAGCTGGCTGGCAGTCTCTTGTGGGGTGAGTCCGGTTTGTCCTCGCTGGTGCGGGGAACTCTAGTTTCCGTATCTCCGGATGTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACGCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATcc                                                    <  2:99298/292‑1 (MQ=255)                                                                                                                                                                                                                                                       gAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCt                                               >  1:468474/1‑299 (MQ=255)                                                                                                                                                                                                                                                         aCTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGgc                                            >  1:348423/1‑300 (MQ=255)                                                                                                                                                                                                                                                         aCTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGCTTCAGTATCCCCGGCTCTCCAAAACTTCCGGCCCATCCACACTAACACGAGGAACTCGCAGACGGCGCCGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCCGACGCGAGAAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAACCGGCTTATCCACGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCACGGTTTAt                                                      >  1:348456/1‑290 (MQ=255)                                                                                                                                                                                                                                                          cTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCCACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGgc                                            >  1:337311/1‑299 (MQ=255)                                                                                                                                                                                                                                                          cTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGgc                                            >  1:337602/1‑299 (MQ=255)                                                                                                                                                                                                                                                                              ctTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTc                        >  2:395506/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                tcaccgtcggtgccGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGttt                                                        <  2:557842/253‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGAACGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCACGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCACGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCCCCtt             >  2:427517/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                              ggTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGc         <  1:32472/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                              ggTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCt        <  1:37749/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   aTCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCAGCTGGCGCGGGGAACTCTAAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCACCACCTTTGGCTTAGGCt  >  2:100761/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   aTCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTACGGATTTATAAAGCTGACGGTTTATCCCAGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCACGATGGCGCGGGGAACTCAGCGTGTTCGGCATCAACTTTGGCTTCGGCt  >  1:590993/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   aTCCTCGCTGGCGCGGGGAACTCTAGGTTCCGTCTCTACGGATTTCTAACGCTGACGGTTTATCCCAGCTGGCGCGGGGAACTCGCAGGCAGCGACGCGCAGGGTATGCGCGATTCGCGATTCACCAACGCGGGCAAGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACAATTTATCCCCACTGGCACGGGGAACTCTAAAAATTGCCACTTCCAACCGACAGGGAGCCCGGTTTGTCCACGCTGGCGCGAGGAACTCAGTGTATTCGGCATCCCCCTTGGATTCGGCt  >  2:100766/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        GGGAACTCTCGGTTCAGGCGTTGCAAACCTGGCTACCGGGCGGTTTATCCCCGCTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCCCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGGAGCCCGGTTTATCCCCGCTGGCGCGGGGAACTCAGCGTGTTCGGCATCACCTTTGGCTTCGGCT  >  NC_000913/2877783‑2878372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑