Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,850	G→A	noncoding (11/77 nt)	argQ ←	tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,850	0	G	A	88.5%	164.3 / 7.7	61	noncoding (11/77 nt)	argQ	tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base A (18/36);  total (20/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAG‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCT  >  NC_000913/2817562‑2818127                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                       gCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATTAGATATTTTTGAAGGAAATTTTTTGGAGAAGATNGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGTCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCa                                                                                                                                                                                                                                                                            <  2:482890/300‑1 (MQ=255)                 aagaaAATTCAGGAAATNGATAAAGGAGTTATTTAGNAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCNTCGGGAAAACTTATTc                                                                                                                                                                                                                                                            <  1:514171/300‑1 (MQ=255)                       aTTCAGGAAATGGATAAAGGAGTTATTTAGAAATGGGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTActgc                                                                                                                                                                                                                                                      <  1:608911/300‑1 (MQ=255)                              aaaTGGATAAAGGAGTGATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATAtt                                                                                                                                                                                                                                               <  2:516238/300‑1 (MQ=255)                                   gATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACGGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATAc                                                                                                                                                                                                                                          <  2:19559/300‑1 (MQ=255)                                          gAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAG‑CTACGGATGCATCGGGAAA‑TTTATTTTACTGCTGATATTGATACCGctacta                                                                                                                                                                                                                                  >  1:585738/1‑300 (MQ=255)                                              tATTTAGAAATTAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGNTTACGGAGAATATGGTGCATCCGGGAGGATTCNAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAg                                                                                                                                                                                                                               <  2:457774/300‑1 (MQ=255)                                               aTTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGc                                                                                                                                                                                                                              <  1:304183/300‑1 (MQ=255)                                                tttAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCNTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGcc                                                                                                                                                                                                                             <  1:447695/300‑1 (MQ=255)                                                tttAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGTGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTNACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGcc                                                                                                                                                                                                                             <  2:444215/300‑1 (MQ=255)                                                              atTTTTGAAGGAAATTTTTTGGAGGAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGTTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAG‑CTACGGATGCATCGGGAAA‑TTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAgaga                                                                                                                                                                                                              <  2:271718/300‑1 (MQ=255)                                                                          aaTTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCg                                                                                                                                                                                                   <  1:475494/300‑1 (MQ=255)                                                                              ttttGTAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCNTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGggag                                                                                                                                                                                               <  1:135898/300‑1 (MQ=255)                                                                                      aagaTGGTGCATCCGGGAGGATGACTCACTTACGTTCGCCCTTCGTGCCGTTGCTGTCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGTAGGATTCGAACCTCNGACCGCTCGGTTCGTAGCCGAGTACTCTATCCNGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTc                                                                                                                                                                                       <  2:469486/300‑1 (MQ=255)                                                                                      aagaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGTAGGATTACTc                                                                                                                                                                                       <  1:409737/300‑1 (MQ=255)                                                                                      aagaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTc                                                                                                                                                                                       <  1:10421/300‑1 (MQ=255)                                                                                        gaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTc                                                                                                                                                                                       <  2:515814/298‑1 (MQ=255)                                                                                           ggTGCATCCGGGAGGATGGCTCACTGATGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCATTCCTGGTGCCTGCGGTTAATGCCGCTACCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAG‑CTACgg                                                                                                                                                                                                                                                                                 <  1:125275/205‑1 (MQ=255)                                                                                                aTCCTGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTTGCGCAACTTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCTGGAGGATTCGAACCTCCGACCGCTCGNTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTANGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGc                                                                                                                                                                             <  2:461694/300‑1 (MQ=255)                                                                                                aTCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAATAGATGGTGCATCCGGGAGGATTACTCGCCTTGCgg                                                                                                                                                                              <  1:543241/299‑1 (MQ=255)                                                                                                        ggaTGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTACTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTACGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTCATTCTCCTGCTGCTCTTGATAACTCTACCAAAACCGGACCCAGTCAAAGATGGTGCATCCAGGAGGGTTACCCGCCCTGCAGCTTGCCCtt                                                                                                                                                                     >  2:83946/1‑300 (MQ=17)                                                                                                              cTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGCAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAG‑CTACGGATGCATCGGGAAA‑TTTATTTTCCTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGAttcgaacctccgaccgctcggttcgtagcc                                                                                                                                                               >  1:159064/1‑271 (MQ=11)                                                                                                               tcccTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATGCCGCTACCTCTTCGATNCCGTCATTGCTGAAGATTGCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAG‑CTACGGATGCATCGGGAAA‑TTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAagag                                                                                                                                                                                                               <  2:194720/247‑1 (MQ=11)                                                                                                               tCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGcc                                                                                                                                                               <  1:369472/299‑1 (MQ=255)                                                                                                                   tGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTGCGATACCTTCATTGCGGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTg                                                                                                                                                           <  1:335039/299‑1 (MQ=255)                                                                                                                      cGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTACTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCAGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGaaatctcctt                                                                                                                                                                                                                                                             >  2:10051/1‑191 (MQ=255)                                                                                                                         tCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTNATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCTGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGc                                                                                                                                                    <  1:112257/300‑1 (MQ=255)                                                                                                                                tCGGGCCGTTACTGGCACAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTACTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGc                                                                                                                                                    <  2:99375/293‑1 (MQ=255)                                                                                                                                            tGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTg                                                                                                                                 <  2:562181/300‑1 (MQ=255)                                                                                                                                              gcgcGACGTTATCCTTTCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATGCCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCTGGAGGATTCGAACCTCCTACCGCTCGGTTCTTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACCTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTg                                                                                                                                 <  2:171/298‑1 (MQ=21)                                                                                                                                              gcgcAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGcc                                                                                                                               <  1:357205/300‑1 (MQ=255)                                                                                                                                                  aaCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCGTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCTGTAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACGTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                                                                                                                            <  2:315647/299‑1 (MQ=255)                                                                                                                                                   aCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                                                                                                                            >  1:90984/1‑298 (MQ=255)                                                                                                                                                   aCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTAATGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                                                                                                                            >  1:336019/1‑298 (MQ=255)                                                                                                                                                        aTCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCGGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                                                                                                                     <  1:460681/300‑1 (MQ=255)                                                                                                                                                        aTCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCTGGGGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                                                                                                                     <  1:460941/300‑1 (MQ=255)                                                                                                                                                            ttcctGGTCCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAATCGCCCTGCGGCTCGCACTTAGGGCCGTTGCTAAAGTAAAGTTATCATCCAGAGTGCCTGCGGTTAATTcc                                                                                                                  >  1:6733/1‑299 (MQ=18)                                                                                                                                                             tcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCTGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAATAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc                                                                                                                <  2:29037/300‑1 (MQ=255)                                                                                                                                                                        gCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGTTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                                                                                     >  1:394430/1‑300 (MQ=255)                                                                                                                                                                          ggTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGAt                                                                                                    <  1:486350/299‑1 (MQ=255)                                                                                                                                                                                      tATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGc                                                                                         <  1:142172/298‑1 (MQ=255)                                                                                                                                                                                         ctctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGaaacct                                                                                                                                                                                                                                                                 >  1:422078/1‑124 (MQ=255)                                                                                                                                                                                           ctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCACTACTAAAGCCATGTCCATTAAGAAGTGGTGCATCCGGGGGGATTAATCGCCTTGCGGCTCGCCCTTCGGGCCGTTGATCAAGCAATTTTATCCTCACTATTACCTGCGGTTCCTTCCGATGCCTCTTCTATCCCTTCATTGCTGagggt                                                                                  >  2:365019/1‑296 (MQ=17)                                                                                                                                                                                            ttCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAt                                                                                  <  1:528133/299‑1 (MQ=255)                                                                                                                                                                                                          tgctgaatgctgctgggaATATGGTGCATCCTGGAGGATTCGAACCTCCTACCGCTCGGTTTTTAGCCGGGTGCTCTATCTA‑TTCAGACTACGAAAGCACCGGAAAAACGTATTCTACTTATGGTATGAATACCGCTACTGGAGTTAGATTGATTGGGAGATGGGGCATCTTTGGGGGGGACGCGCCTTGCTGTTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt                                                                   <  1:596920/284‑1 (MQ=255)                                                                                                                                                                                                           gCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTCCTGCTGATATTGATCCCGCTACTAAAGCCATCTCAATTAAGAGATGGTTCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTCCAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCCCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt                                                                   >  1:291529/1‑299 (MQ=21)                                                                                                                                                                                                                 tgtgactgagaATATTGTGCGTTCGGAAGGATTCAAATCTCCGACCGCTCAGTTCTTAGCTTGTTACTCTGTTCGACGGAG‑CGACAGATGCATTGGGAAGATTTATTCTATTTTTGGCATTGATACCTCTGTTAAAGCCATGTTCGTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCg                                                            <  2:601398/293‑1 (MQ=12)                                                                                                                                                                                                                  gTTACGGAGAATATGGTGCATCCGGGAGGATTCGCCCCTCCGACCGCTCGGTTCGTAGCCGAGTCCTCTATCCAGCTGAA‑CTAAGGATGCATCGGGAAAATCTATTCCACTGCTGATATTGATACCGCTACTAAAGCAATATCAATTAAGAGATGGTGCATTCGGGAGGATTAATCGCCTTGCGTCTCGCCCTTCGGGCCGTTGCTAAAACAACGTTATCATCAATAGTGCCTGCGGTTAATTCCGCTAATTATTCGCTACATTCATTGCTGAAGGTTTAGGAGAATGTGGTGCATc                                                              >  2:237092/1‑297 (MQ=255)                                                                                                                                                                                                                       ggaggaTATGGTGCATCCGGGAGGATTCGAACCTCCGATCGCTCGGTTCGTAGCCGAGCGCTCTATCCAGCGGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAGGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagg                                                       <  1:180770/299‑1 (MQ=255)                                                                                                                                                                                                                          gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATGTTGATACCGCTACCAAAGCCATCTCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGccc                                                                                                                                                                       >  2:595603/1‑184 (MQ=255)                                                                                                                                                                                                                          gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTACA‑CTACAGATGCATCGGAAAAACTTATTCTACTACTGATATTGCTACCGCTACTCAAGCCATATCCATTCAGCGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGATCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTAATTGCTGACGATTTCGGAGGACATGGTGCATCCGGGAGGATTc                                                   >  2:42026/1‑300 (MQ=18)                                                                                                                                                                                                                                     aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCTAACCTCCGa                                         >  1:374802/1‑299 (MQ=255)                                                                                                                                                                                                                                                      cctacGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATGACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCATAGTGCCttctattacctccac                                                                                                                >  2:413950/5‑197 (MQ=17)                                                                                                                                                                                                                                                                   ggTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc           <  1:42026/299‑1 (MQ=255)                                                                                                                                                                                                                                                                   ggTTCATAGCCGAGTACTCTATCTAGCGGAA‑CTACGGATGCATCGGGAAAACCTAGTCTACTGCTGATATTAATACCNCTACTAAAGCCACATCAATTAAGAGATGGTGCATCCTGGAGGAGTACGCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAGAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCAGAGTACTCTATcc           <  1:445821/299‑1 (MQ=18)                                                                                                                                                                                                                                                                    gTTCGTAGCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCATAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCa          >  2:538777/1‑299 (MQ=255)                                                                                                                                                                                                                                                                           gCCGAGTACTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt  >  1:381511/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  aCTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGgga                                                         >  1:614750/1‑238 (MQ=255)                                                                                                                                                                                                                                                                                  aCTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGgga                                                         >  1:613193/1‑238 (MQ=255)                                                                                                                                                                                                                                                                                  aCTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGgga                                                         <  2:613193/238‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aCTCTATCCAGCTGAA‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGgga                                                         <  2:614750/238‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                       GCTGATGGCGAAGGAGAAGAAAATTCAGGAAATGGATAAAGGAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAG‑CTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCT  >  NC_000913/2817562‑2818127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑