Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,792,209	G→A	V313M (GTG→ATG)	gabD →	succinate‑semialdehyde dehydrogenase (NADP(+)) GabD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,792,209	0	G	A	100.0%	149.9 / NA	42	V313M (GTG→ATG)	gabD	succinate‑semialdehyde dehydrogenase (NADP(+)) GabD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/21);  total (21/21)

GTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAGTGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGC  >  NC_000913/2791922‑2792488                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                        gtAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGc                                                                                                                                                                                                                                                                             >  2:578619/1‑300 (MQ=255)      gAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATc                                                                                                                                                                                                                                                                        >  1:193506/1‑300 (MQ=255)         cTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTCTGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCATATCGGc                                                                                                                                                                                                                                                                     >  2:170375/1‑300 (MQ=255)          tGACCAGTAATCCGCTGGTGCGCAAGCTGTCGTTTACCGGTTCGACCGAAATCGGCCACCAGGTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCTTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCT‑CGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACTGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATc                                                                                                                                                                                                                                                                        <  2:396934/295‑1 (MQ=255)           gACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAGTTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGAAAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGa                                                                                                                                                                                                                                                                   >  2:60960/1‑300 (MQ=255)                          ggTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGTCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAACCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACgg                                                                                                                                                                                                                                                    <  2:529695/300‑1 (MQ=255)                                 aaaCTGTCGTTTACCTGGTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCNGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCAcc                                                                                                                                                                                                                                             <  2:475584/300‑1 (MQ=255)                                 aaaCTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAATACATCAAGAAAGTGTCGCTGGAGCTGGGCNGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCAcc                                                                                                                                                                                                                                             <  2:475591/300‑1 (MQ=255)                                  aaCTGTCGTTTACCGGTTCGACCGAAATTTGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAANGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCa                                                                                                                                                                                                                                            <  2:199344/300‑1 (MQ=255)                                  aaCTGTCGTTTACCGGTTCGACCGAAATCGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAANGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCa                                                                                                                                                                                                                                            <  2:199716/300‑1 (MQ=255)                                         gTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCACCGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGTCTGAATAACGGCGTCACCATCgggac                                                                                                                                                                                                                                     >  2:281142/1‑298 (MQ=255)                                         gTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGcc                                                                                                                                                                                                                                     >  1:256344/1‑300 (MQ=255)                                                                    ccAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCaaa                                                                                                                                                                                                          >  1:295725/1‑300 (MQ=255)                                                                         tAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGCTGAAAAAGCGGTAGCAAAAGt                                                                                                                                                                                                       >  1:600307/1‑298 (MQ=255)                                                                         tAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGt                                                                                                                                                                                                       >  1:600455/1‑298 (MQ=255)                                                                           aTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGCGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAANCTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGaa                                                                                                                                                                                                   >  1:444793/1‑300 (MQ=255)                                                                                   gTGCGCGAAAGACATCNAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGANCGATGAAAAAGCGGTAGCNAAAGTGGAAGAGCatat                                                                                                                                                                                           >  1:512425/1‑300 (MQ=255)                                                                                       gcgAAATACAGCAATAAAGTGTCACTGGAGTTGTGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCGGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGc                                                                                                                                                                                        <  2:369676/299‑1 (MQ=255)                                                                                       gcgAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACACGCCGTTTATCGTCTGTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGc                                                                                                                                                                                        <  2:250894/299‑1 (MQ=255)                                                                                                   aaGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGgag                                                                                                                                                                           <  1:615185/300‑1 (MQ=255)                                                                                                                        ggCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTgc                                                                                                                                                      >  1:319347/1‑300 (MQ=255)                                                                                                                            gTAACGCGCCGCTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGAACGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATACCGGCGTCACCATCGGGCCGCTGATCGATGCAAAAGCGGTAGCAAAAGTGGAAGAGTATATTGCCGATGCGATGGAGAAAGGCGCGTGCGTGGTTTgcggc                                                                                                                                                   >  2:279880/1‑299 (MQ=255)                                                                                                                                          aTCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCATGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGaa                                                                                                                                    <  1:603297/300‑1 (MQ=255)                                                                                                                                          aTCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGaa                                                                                                                                    <  1:237897/300‑1 (MQ=255)                                                                                                                                              tCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCCCCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCAGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAAcgc                                                                                                                                 >  2:156257/1‑299 (MQ=255)                                                                                                                                                                        gCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCAtt                                                                                                      >  2:367754/1‑300 (MQ=255)                                                                                                                                                                        gCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCAtt                                                                                                      >  1:526540/1‑300 (MQ=255)                                                                                                                                                                                                    tCCGCAACGCCGTGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAg                                                                          <  2:38365/300‑1 (MQ=255)                                                                                                                                                                                                    tCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTTCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAg                                                                          <  2:418379/300‑1 (MQ=255)                                                                                                                                                                                                    tCCGCAACACCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTGTGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAACCTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGACGAAAAAGCGGTAGCAAAAGTGGAAGAGCGTATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAg                                                                          <  2:37929/300‑1 (MQ=255)                                                                                                                                                                                                                   aaaCCTGCGGCTGCGCCAACCGCCTGTATGTGCAGGACTGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGANGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAagag                                                            <  2:452392/299‑1 (MQ=255)                                                                                                                                                                                                                    aaCCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGAc                                                          <  1:488822/300‑1 (MQ=255)                                                                                                                                                                                                                     aCCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCAGGCCAACGCCAAAGTGTCGAAAGAAGAGAc                                                          >  2:546263/1‑299 (MQ=255)                                                                                                                                                                                                                     aCCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCAGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGAc                                                          >  2:248971/1‑299 (MQ=255)                                                                                                                                                                                                                                        gCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGc                                      >  2:562749/1‑300 (MQ=255)                                                                                                                                                                                                                                             tATGTGCAGGACTGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGGGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAATAGACGTTCGGCCCCCTCGCCCCGCTGTTc                                 <  2:256344/300‑1 (MQ=255)                                                                                                                                                                                                                                             tATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTc                                 <  1:543528/300‑1 (MQ=255)                                                                                                                                                                                                                                                         ggCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATNAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTNCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGAt                     >  2:456994/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gtgtATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGaa                  >  1:58351/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   aCCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGAtg           <  1:43630/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCCTTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTgcgc  <  2:536447/298‑1 (MQ=255)                                                                                                                                                                                                                                                                              gAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTgcgc  <  1:254149/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                        GTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAGTGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGC  >  NC_000913/2791922‑2792488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑