Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,792,209	G→A	V313M (GTG→ATG)	gabD →	succinate‑semialdehyde dehydrogenase (NADP(+)) GabD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,792,209	0	G	A	100.0%	178.8 / NA	52	V313M (GTG→ATG)	gabD	succinate‑semialdehyde dehydrogenase (NADP(+)) GabD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/18);  total (34/18)

GTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAGTGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGA  >  NC_000913/2791918‑2792504                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                        gtcggtAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCaaa                                                                                                                                                                                                                                                                                                 >  1:812311/1‑300 (MQ=255)               gACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCATGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATTGGCGa                                                                                                                                                                                                                                                                                   >  2:58384/1‑300 (MQ=255)                           gCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGAt                                                                                                                                                                                                                                                                         <  2:584456/298‑1 (MQ=255)                                       aCTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCAt                                                                                                                                                                                                                                                           <  1:719629/300‑1 (MQ=255)                                                                               aTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGCCCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGaa                                                                                                                                                                                                                   >  1:712559/1‑300 (MQ=255)                                                                               aTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGaa                                                                                                                                                                                                                   >  1:273923/1‑300 (MQ=255)                                                                               aTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGaa                                                                                                                                                                                                                   >  2:722306/1‑300 (MQ=255)                                                                                           gctaaaGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGc                                                                                                                                                                                                        <  1:596503/296‑1 (MQ=255)                                                                                           gcgAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGc                                                                                                                                                                                                        <  2:882856/299‑1 (MQ=255)                                                                                             gAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCg                                                                                                                                                                                                      <  1:423677/299‑1 (MQ=255)                                                                                                   cATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACANCGGCGACGGGCTGGATAACGGCGTCAACATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCAGATGCGCt                                                                                                                                                                                               >  2:699659/1‑300 (MQ=255)                                                                                                       aaGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCTACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGCGGAAGAGCATATTGCCGATGCGCTGgag                                                                                                                                                                                           <  2:832234/300‑1 (MQ=255)                                                                                                       aaGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGgag                                                                                                                                                                                           <  2:812311/300‑1 (MQ=255)                                                                                                          aaaGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGaaa                                                                                                                                                                                        <  2:297359/300‑1 (MQ=255)                                                                                                           aaGTGTCGCTGGAGCTGTGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCGCGACAAGGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTTCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCATGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGTAAGAGCATATTGCCGATgctctttatccc                                                                                                                                                                                        <  2:298702/299‑11 (MQ=255)                                                                                                            aGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAgg                                                                                                                                                                                      <  1:484139/300‑1 (MQ=255)                                                                                                                 cGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGgcgcgc                                                                                                                                                                                 >  1:602824/1‑300 (MQ=255)                                                                                                                           gggCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTNTGCGCCAACCGCCTGTATGTGCAGGACGGCGTNTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGttt                                                                                                                                                                        >  2:669261/1‑299 (MQ=255)                                                                                                                           gggCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAACGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGTTGGAGAAAGGTGCGCGTGTGGttt                                                                                                                                                                        >  2:363337/1‑299 (MQ=255)                                                                                                                                gTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACTGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTgcggc                                                                                                                                                                   >  2:74605/1‑299 (MQ=255)                                                                                                                                          gTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCNAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGATAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAgcgc                                                                                                                                                         <  1:632980/299‑1 (MQ=255)                                                                                                                                                 gTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTNGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAAcgc                                                                                                                                                 >  1:639529/1‑300 (MQ=255)                                                                                                                                                  tCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAACAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAAcgc                                                                                                                                                 >  1:717061/1‑299 (MQ=255)                                                                                                                                                  tCTTTGACGATGCCGACCTAGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATAGGGCCGATGATCGATGAAAAAGCAGTAGCAAAAGTGGCAGGGTATCTTGCAGATGTGCTGGAGAAAGGCGAGCGCGTGGTTTGCGGCGGTAAAGCGCACGAAca                                                                                                                                                  >  2:433656/1‑297 (MQ=255)                                                                                                                                                                     cGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGCACGCGGCGGCAACTTCTTCCAGcc                                                                                                                             >  1:96970/1‑300 (MQ=255)                                                                                                                                                                     cGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGcc                                                                                                                             >  1:97723/1‑300 (MQ=255)                                                                                                                                                                          aaGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTACAGCCGACCa                                                                                                                        >  2:752299/1‑300 (MQ=255)                                                                                                                                                                            gCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCAt                                                                                                                       >  2:718404/1‑299 (MQ=255)                                                                                                                                                                            gCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGATGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCAtt                                                                                                                      >  2:765558/1‑300 (MQ=255)                                                                                                                                                                            gCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGANGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCAtt                                                                                                                      >  1:169227/1‑300 (MQ=255)                                                                                                                                                                                  gAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCNTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCtggtg                                                                                                                <  1:166155/300‑1 (MQ=255)                                                                                                                                                                                  gAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCtggtg                                                                                                                <  2:603692/300‑1 (MQ=255)                                                                                                                                                                                     ggCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTNCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGAc                                                                                                             <  2:699809/300‑1 (MQ=255)                                                                                                                                                                                              gctgcGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCACCTGTATGGGCAGGACGGCATGTATGACCGTGTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGTGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGcc                                                                                                    <  2:162535/296‑1 (MQ=255)                                                                                                                                                                                                                         aCCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAAAGAc                                                                          >  2:20833/1‑299 (MQ=255)                                                                                                                                                                                                                                          ccGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGcccc                                                        >  1:617026/1‑300 (MQ=255)                                                                                                                                                                                                                                               tGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCg                                                       >  1:201764/1‑296 (MQ=255)                                                                                                                                                                                                                                                gTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGNCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTNGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGtt                                                  >  2:668873/1‑300 (MQ=255)                                                                                                                                                                                                                                                 tATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGCTGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTc                                                 >  2:331544/1‑300 (MQ=255)                                                                                                                                                                                                                                                 tATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGCCCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCACCCTCGCACCGCTGTTc                                                 >  2:331530/1‑300 (MQ=255)                                                                                                                                                                                                                                                 tATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTc                                                 <  1:74605/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAGGTGTCGAAAGAAGAGGCGTTCGGCCCCCTCGCCCCGCTGTTCCGc                                              >  2:734362/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGc                                              >  2:880665/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGACGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGc                                              >  1:538280/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATAAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGc                                              >  2:64693/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gtgCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCAGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCACCCTCGCCCCGCCGTTcc                                                >  1:492932/1‑298 (MQ=255)                                                                                                                                                                                                                                                      gCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAGAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACTATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGGCGAAAGAAGGGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCtt                                            >  1:505047/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAACCGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTACAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCACAGTGTCGAAAGAAGAGAAGttaggaacaatagaccagatgttccgctt                                            >  2:1778/1‑273 (MQ=255)                                                                                                                                                                                                                                                                gtgtATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGaa                                  >  1:798210/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gtgtATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCAGCTGTTCCGCTTTAAAGATGaa                                  >  1:37242/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   tATGACCGTTTTGCCGAAAAATTGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCt                               <  2:595137/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        ttGCAGCAGGCAATGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAATAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAAt          <  1:471223/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                ggcaaTGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGa  >  1:19543/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                        GTCGGTAACGAACTGACCAGTAACCCGCTGGTGCGCAAACTGTCGTTTACCGGTTCGACCGAAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGCCTGTATGTGCAGGACGGCGTGTATGACCGTTTTGCCGAAAAATTGCAGCAGGCAGTGAGCAAACTGCACATCGGCGACGGGCTGGATAACGGCGTCACCATCGGGCCGCTGATCGATGAAAAAGCGGTAGCAAAAGTGGAAGAGCATATTGCCGATGCGCTGGAGAAAGGCGCGCGCGTGGTTTGCGGCGGTAAAGCGCACGAACGCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGA  >  NC_000913/2791918‑2792504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑