Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,756,976	G→A	R273Q (CGA→CAA)	intA →	integrase IntA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,756,976	0	G	A	100.0%	317.0 / NA	94	R273Q (CGA→CAA)	intA	integrase IntA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/48);  total (46/48)

AGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCGAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGATGTCATTGAAGCAGCGCTTGCTCATGTAGACAAAAATG  >  NC_000913/2756685‑2757260                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                              aGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAg                                                                                                                                                                                                                                                                                        <  1:283412/299‑1 (MQ=255)    gTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGa                                                                                                                                                                                                                                                                                    >  2:578944/1‑300 (MQ=255)             aaGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGAcc                                                                                                                                                                                                                                                                           <  1:656323/300‑1 (MQ=255)               gTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCAt                                                                                                                                                                                                                                                                         >  2:356922/1‑300 (MQ=255)                  aTGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATAc                                                                                                                                                                                                                                                                       <  2:170595/299‑1 (MQ=255)                    gATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGt                                                                                                                                                                                                                                                                    >  2:748934/1‑300 (MQ=255)                    gATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGt                                                                                                                                                                                                                                                                    <  1:598679/300‑1 (MQ=255)                           gcgcAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTa                                                                                                                                                                                                                                                             <  1:54432/300‑1 (MQ=255)                           gcgcAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTAAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTa                                                                                                                                                                                                                                                             <  1:831930/300‑1 (MQ=255)                                gAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAAACATCACCTGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGa                                                                                                                                                                                                                                                        >  1:491352/1‑300 (MQ=255)                                 aaCACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGAt                                                                                                                                                                                                                                                       <  1:356922/300‑1 (MQ=255)                                 aaCACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGAt                                                                                                                                                                                                                                                       <  2:617013/300‑1 (MQ=255)                                  acacAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGAt                                                                                                                                                                                                                                                       >  1:576192/1‑299 (MQ=255)                                   cccaGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGAt                                                                                                                                                                                                                                                       >  2:849425/3‑298 (MQ=255)                                       ggCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGct                                                                                                                                                                                                                                                 >  1:616664/1‑300 (MQ=255)                                       ggCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCACACGGTTCCACTATCTGATGGGGct                                                                                                                                                                                                                                                 >  1:33792/1‑300 (MQ=255)                                       ggCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTACACAAGATGCCT‑GTTCATG‑TGGCAACTTATAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGct                                                                                                                                                                                                                                                 >  2:485124/1‑300 (MQ=255)                                            gattgatGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCNTCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCtt                                                                                                                                                                                                                                              >  2:264513/1‑298 (MQ=255)                                            gattgatGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGc                                                                                                                                                                                                                                            >  1:432927/1‑300 (MQ=255)                                                gatGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTAtt                                                                                                                                                                                                                                        >  2:134414/1‑300 (MQ=255)                                                 atGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTc                                                                                                                                                                                                                                       >  2:611614/1‑300 (MQ=255)                                                      gTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATTGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGaa                                                                                                                                                                                                                                  <  2:432927/300‑1 (MQ=255)                                                           tAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑ACATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCAACCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGATCAAATGAAGATGAACCGGGACCATACGGTTCAACTATCTGATGGGGATCTTGTCATTCTGGAAat                                                                                                                                                                                                                                >  2:346556/1‑297 (MQ=255)                                                            aGTGTAAATATCGGAANAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCNCTATCTGATGGGGCTCTTNCTATTCTGGAAatgat                                                                                                                                                                                                                             >  1:747581/1‑299 (MQ=255)                                                            aGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAatgat                                                                                                                                                                                                                             >  1:52098/1‑299 (MQ=255)                                                             gtgtAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAAtgatga                                                                                                                                                                                                                           >  1:654570/1‑300 (MQ=255)                                                                 aaaTATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATTGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGAGGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGTAAATGATGAAGcc                                                                                                                                                                                                                       <  2:750123/300‑1 (MQ=255)                                                                        ggAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCTGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCNAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAg                                                                                                                                                                                                                 <  2:685827/299‑1 (MQ=255)                                                                          aaaaGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATNCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAgtgg                                                                                                                                                                                                              <  1:625519/300‑1 (MQ=255)                                                                               cTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTAAGTGGTGGcc                                                                                                                                                                                                         >  1:865070/1‑300 (MQ=255)                                                                                                              aaGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGc                                                                                                                                                                           <  1:764067/299‑1 (MQ=255)                                                                                                                gCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGccc                                                                                                                                                                         >  2:523700/1‑299 (MQ=255)                                                                                                                                       cAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCACAACCAACCAATGAATAGCCAA‑ACa                                                                                                                                                >  2:839626/1‑300 (MQ=255)                                                                                                                                            aaTGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGTTATTCTGGAAATGATGANGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGaa                                                                                                                                           >  1:179066/1‑300 (MQ=255)                                                                                                                                            aaTGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGANGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGaa                                                                                                                                           >  1:171976/1‑300 (MQ=255)                                                                                                                                            aaTGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTACTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGaa                                                                                                                                           >  2:245149/1‑300 (MQ=255)                                                                                                                                            aaTGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCAACCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTAAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGaa                                                                                                                                           >  1:103211/1‑300 (MQ=255)                                                                                                                                             aTGCACACCATGCGTACTGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAAt                                                                                                                                          <  1:754121/300‑1 (MQ=255)                                                                                                                                             aTGCACACCATGCGTACNGCAAGTATCA‑‑GCATGTCCNCAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGANTAGCCAA‑ACAGTGAAt                                                                                                                                          <  1:748934/300‑1 (MQ=255)                                                                                                                                             aTGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAAt                                                                                                                                          >  1:536445/1‑300 (MQ=255)                                                                                                                                               gaacacCAGGCGGACGGCAAGTATCA‑‑GAATGGCCACAAGATGCCT‑GTTCATT‑TGGCAACTTCTAACCATCACCCTCCCTTCCGAAACTGCTGAGGCTCGATGGGATGAGATCGATTTCAATACTAGCAAATGGAAGATTCCTGCGGCTCAAATGAAGATGCACCTGGACCATACGGTTCCACTATCGGATGGGGCTCTGGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGGGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATgc                                                                                                                                        <  2:580219/298‑1 (MQ=255)                                                                                                                                               gCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCAGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATgc                                                                                                                                        >  2:225836/1‑300 (MQ=255)                                                                                                                                                    agaggcggaCGGCAAGTATCA‑‑GCATGTCCACAATATACAT‑GGGCACG‑GTGCAACTTATCACCATCACCCACCCTGCCGAATCTGCTGAGGCTCGATGGGAGGAGATCGATGGCAAGGCTAGCAAATGGAGAATTCTTGTAGCTCAAATGAAAATAAACCGCTACCATAAGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGCATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCAc                                                                                                                                   <  2:52098/292‑1 (MQ=255)                                                                                                                                                          tacgcgCAAGTATCAGNGCATGTCCACAAGATGCCNCGTTCATGGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTGGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAANACAGTGAATGCAGCAc                                                                                                                                   <  1:733232/294‑1 (MQ=255)                                                                                                                                                          gTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCTGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAg                                                                                                                              <  2:747581/299‑1 (MQ=255)                                                                                                                                                          gTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAg                                                                                                                              <  1:10131/299‑1 (MQ=255)                                                                                                                                                          gTACGGCAAGTATCA‑‑ACATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCGAACCACCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCAAATGGAAGATTCCTGCAGCTCAAATGAAGATGAACCGGTACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAg                                                                                                                              <  2:711218/299‑1 (MQ=255)                                                                                                                                                           tgcgttgaGTATCA‑‑GCAGGTCCGCAAGATGACT‑GGTCAGG‑GGGAAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCGGAGGCTCGATGGGATGAGATCGATTTCAATACTAGCGAATGTAAAATTCCTGCAGCTCAAATGAAGACGAACCGTTACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCGCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCGAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCg                                                                                                                            <  2:92731/293‑1 (MQ=255)                                                                                                                                                           tACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCTGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCg                                                                                                                            <  1:264513/300‑1 (MQ=255)                                                                                                                                                           tACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCATCTAAGTGGTGGCCGAGGATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCAAA‑AAAGTGAATGCAGCACTCAAGc                                                                                                                             >  2:45628/1‑299 (MQ=255)                                                                                                                                                              ggCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCTGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑Acctgtctcttatacacatctcgcag                                                                                                                         <  1:709793/300‑25 (MQ=255)                                                                                                                                                                   gTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTc                                                                                                                                                                                                                                       <  1:265444/186‑1 (MQ=255)                                                                                                                                                                   gTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGTCGTATCAAGCCCAAACAACCAATGAATGGCCAA‑ATAGTGAATGTAGCATTCAAGCGTGCTGGCt                                                                                                                    >  2:189269/1‑300 (MQ=255)                                                                                                                                                                   gTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCAGTATCAAGCCCAACCAACAAATGAATAGCAAA‑AAAGTGAATGCAGCAATCAAGCGTGCTGGCt                                                                                                                    >  2:190669/1‑300 (MQ=255)                                                                                                                                                                   gTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTAAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCACAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCt                                                                                                                        >  2:19919/1‑296 (MQ=255)                                                                                                                                                                   gTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCNGATGGGGCTCTTGCTATTc                                                                                                                                                                                                                                       >  2:265444/1‑186 (MQ=255)                                                                                                                                                                        a‑‑GCATGTCCGCGAGATACCT‑GTTCATG‑TGGCAACTCAGAACCAGCACCCGCCCTGCCGAGGCTGCGTAGGCTCGATGGGATGAGACCGACTCCAATGCTAGCAAATGGAAAATTCCTGCAGCTCAAGTTAAGATGGATCGGGACCATACTGTTCCACTATCTGANGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAAGGCAGCACTCAAGCGTGCTGGCTTagga                                                                                                               <  2:644430/300‑1 (MQ=255)                                                                                                                                                                        a‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTagga                                                                                                               <  1:314293/300‑1 (MQ=255)                                                                                                                                                                        a‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGACTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGANGGGGCTCTTGCTATTCTGGAAATGATGAAGCCNCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTagga                                                                                                               <  2:661768/300‑1 (MQ=255)                                                                                                                                                                        a‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCACCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCGATGCTAGCGAAGGGAAAATTCCTGCAGCTCAAATTAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTagga                                                                                                               <  2:541722/300‑1 (MQ=255)                                                                                                                                                                        a‑‑GCATGTCCACAAGATACCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGANGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTagga                                                                                                               <  2:644439/300‑1 (MQ=255)                                                                                                                                                                          aggggggcaacaagagaccT‑GTTTATG‑AGGCAACGTATAACCACAACCTACCCTGCTTAAGCTGTTGAGGCTTGATTGGATGAAATCGATTTCAATGCTAGCGAAGGGAAAACTCCTGCAGATCACATTGATATGGGCTGAGACCATACGCTCCCACCCTCTGATTGGACTCTTGTTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAATCAATGAACAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCGTagga                                                                                                               <  2:250266/283‑1 (MQ=255)                                                                                                                                                                          aacATGTCCACAAGATGAAT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCGGCCGAAGCTGCTGAGGCTCGATTGGATGAGATCGACTTCAATGCTAGAAAATGGAAAACCCCTGCAGCTCAAATTAAGACAAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTGATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTagga                                                                                                               <  2:2249/298‑1 (MQ=255)                                                                                                                                                                             aggtgcagagggtGCCG‑GTTCGTG‑TGGCAACTCATAACCACCACCCGTCCTATCGAAGCTGCTGAGGCTCTAGGTGATGAAAGCGATTCCAATGCGAGCGAATGGGAAACTCCTGCAGCTCAAATGAAGATGAATCGGGACCATACGGTTCCACTATCTGATGGAGCGCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCCTTCCTAGCCGTATCAAGCCCAACCAACCAATAAATAACCAA‑ACAGCGAATGCAGCACTCAAGCGTGCTGGCTTaggagg                                                                                                             <  2:121754/287‑1 (MQ=255)                                                                                                                                                                               gTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGACTTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCAAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGgtgt                                                                                                          >  2:750245/1‑300 (MQ=255)                                                                                                                                                                                   acaAGATGCCT‑GTTCATG‑TTGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCTGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACtt                                                                                                      <  2:522531/300‑1 (MQ=255)                                                                                                                                                                                   acaAGAGGCCT‑GGTCAGG‑TGGCAACGTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCGCGATGGGATGAGATCGATTTCAATGNTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTAc                                                                                                        <  2:683950/298‑1 (MQ=255)                                                                                                                                                                                             t‑GTTCGTG‑TGGCAATTTCTACTCATCACCCACCCTGCCGAAACTACTTATGCTCGATGTGATTAGATCAATTTCAATGCTAGCGAATGGACAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATATGTTTCCACTCTCTGATTGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTAAATGCAGCACTCAAGCGTGCTGGCTTAGTAGGTGTACTTGTTTCacac                                                                                             <  2:327133/299‑1 (MQ=255)                                                                                                                                                                                               gTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCacacac                                                                                           <  1:696730/300‑3 (MQ=255)                                                                                                                                                                                                        ggCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGtt                                                                                   <  1:42457/300‑1 (MQ=255)                                                                                                                                                                                                             cTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTTGCCGGGAATTTATCTTTCATAGCCGTATAAAGCCCAACAAACAAATGAATAGCCAA‑ACAGTGAGTGTAGCAATCAAGCGTGCTGGCTTAGGAGGTGTATTTGTTTCACACGGCTTGCGTTccat                                                                               >  2:202467/1‑296 (MQ=255)                                                                                                                                                                                                             cTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGCGGATTTATCTTTCCTAGCCGTATCGAGCCGAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATc                                                                              >  2:201911/1‑300 (MQ=255)                                                                                                                                                                                                                      aTCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGTAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTAc                                                                      <  1:262408/299‑1 (MQ=255)                                                                                                                                                                                                                      aTCACCCGCCCTGCCGAAGCTGCGGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGTAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTAc                                                                      <  1:261979/299‑1 (MQ=255)                                                                                                                                                                                                                       tCACCCGCCCGGCCGAAGCTGCGGAGGCTCGAGGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGcc                                                                           <  2:746841/293‑1 (MQ=255)                                                                                                                                                                                                                       agacatgCCCTGCCTAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGCATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACgg                                                                    <  2:716174/294‑1 (MQ=255)                                                                                                                                                                                                                        cACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGc                                                                   >  1:851610/1‑300 (MQ=255)                                                                                                                                                                                                                             gCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACgg                                                                    >  1:716174/1‑294 (MQ=255)                                                                                                                                                                                                                             gCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCAc                                                                 <  1:265903/297‑1 (MQ=255)                                                                                                                                                                                                                                  gCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGag                                                         <  1:355808/300‑1 (MQ=255)                                                                                                                                                                                                                                  gCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCAAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCGTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTAAGGCACTCAATGa                                                          >  2:880600/1‑299 (MQ=255)                                                                                                                                                                                                                                     gAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGagga                                                       <  1:45628/299‑1 (MQ=255)                                                                                                                                                                                                                                         ctgctgAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCNTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGAt                                                  >  2:646619/1‑300 (MQ=255)                                                                                                                                                                                                                                           gctgAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTATAGGTGGTGGACGAGGATTTATCTTTCCTAGCCGTATCAAGCCCAAACGAACAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTc                                                                                                 >  2:77636/1‑251 (MQ=255)                                                                                                                                                                                                                                                    cGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGAt                                       >  1:31854/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTAAGTGGTGGCCGCAAATTTATCTTTACTAGCCGTATCAAGCCCAAACAACAAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGATTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTACACCTGAt                                       >  2:468696/1‑299 (MQ=255)                                                                                                                                                                                                                                                        gggATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGATGTCa                                   >  1:838556/1‑300 (MQ=255)                                                                                                                                                                                                                                                         ggATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGATGTCAt                                  >  1:810837/1‑300 (MQ=255)                                                                                                                                                                                                                                                         ggATGAAATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGATGTCAt                                  >  2:806253/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gaTCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGATGTCATTGAagc                            <  1:611614/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    aTTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTANGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGATGTCATTGAagcagtgc                       >  2:699114/1‑297 (MQ=255)                                                                                                                                                                                                                                                                    aTTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTACTAGCCGTATCAAGCACAACCAAACAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTANGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGATGTCATTGAAGCAgcgc                       >  2:699239/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        aaaaTTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGATGTCATTGAAGCAGCGCTTGCTCATGTAGACAAAAAt   <  1:371978/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         aaaTTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGATGTCATTGAAGCAGCGCTTGCTCATGTAGACAAAAATg  <  1:578944/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                              AGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCA‑‑GCATGTCCACAAGATGCCT‑GTTCATG‑TGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCGAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAA‑ACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCCACCTGATGTCATTGAAGCAGCGCTTGCTCATGTAGACAAAAATG  >  NC_000913/2756685‑2757260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑