Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	98.8%	284.2 / NA	81	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (43/37);  minor base T (0/1);  total (43/38)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCTGTGGTCATTGCTGCGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCACAGAAATGTTCCGCAAAGAGTACGCAGAAGTTTTTGAAGGCACAGC  >  NC_000913/1337104‑1337683                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                     gcgcTGTGGTCATTGCTGCGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGACAATCAAAAAAGGCGACTTaa                                                                                                                                                                                                                                                                                             >  2:61952/1‑300 (MQ=255)   gcTGTGGTCATTGCTGCGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGCCACCGTATCTCGACGAACTGGGGTTTAAACTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCACGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAcc                                                                                                                                                                                                                                                                                           >  2:49549/1‑300 (MQ=255)   gcTGTGGTCATTGCTGCGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAcc                                                                                                                                                                                                                                                                                           >  1:828714/1‑300 (MQ=255)      gtgGTCATTGCTGCGATAACCTCGTGCACCAACACCTCTCACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAACAAAGCCGTAACTCTGGGCCTCAAGNGGCAACCATGGGTCAAAGCGTCGCTGGCACCGNGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGCATCTAGACGAACTGGGGTTTAAACTTGTGGGATACGGTTG‑TACCAACTGTATTGGTAACTATGGGCCGCTGCCCGATCCTATCGCAACGGCACTCAAAAAAGGCGATTTAACCGTc                                                                                                                                                                                                                                                                                        >  2:671146/1‑300 (MQ=255)        ggTCATTGCTGCGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTc                                                                                                                                                                                                                                                                                        >  1:86531/1‑298 (MQ=255)          tCATTGCTGCGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                                                                                                                                                                                                                                     >  1:695668/1‑299 (MQ=255)            aTTGCTGCGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggtgc                                                                                                                                                                                                                                                                                   <  1:223199/299‑1 (MQ=255)             ttGCTGCGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggtgc                                                                                                                                                                                                                                                                                   >  1:66004/1‑298 (MQ=255)                 tgcGATGACTTCGTGCACCAACACCTCTAATCTAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCTTAACTCTGTGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTTGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCggtgcggtgc                                                                                                                                                                                                                                                                              <  2:208088/299‑1 (MQ=255)                  gcGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCt                                                                                                                                                                                                                                                                             >  1:37647/1‑299 (MQ=255)                   cGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggtgcggtg                                                                                                                                                                                                                                                                               >  1:655476/1‑296 (MQ=255)                     aTAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAATCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTcc                                                                                                                                                                                                                                                                         <  1:201481/300‑1 (MQ=255)                     aTAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTcc                                                                                                                                                                                                                                                                         <  1:131774/300‑1 (MQ=255)                        aCCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCACGCGGCAACCATGGGTCCAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGACCTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACTTGTATTGGTAACTCTGGGTCGCTGCCCAATCCTATCGAAACAGCAATCAAAAAAGGCGATTTACCCGTCGGTGCGGTGCTGTCCGGc                                                                                                                                                                                                                                                                      >  2:51258/1‑300 (MQ=255)                        aCCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGc                                                                                                                                                                                                                                                                      >  1:831807/1‑300 (MQ=255)                        aCCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGc                                                                                                                                                                                                                                                                      >  2:51324/1‑300 (MQ=255)                                 aCCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAc                                                                                                                                                                                                                                                             >  1:511763/1‑300 (MQ=255)                                 aCCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAc                                                                                                                                                                                                                                                             <  2:592070/300‑1 (MQ=255)                                            tAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTTGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAg                                                                                                                                                                                                                                                      <  1:654881/296‑1 (MQ=255)                                             aaCCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGt                                                                                                                                                                                                                                                 >  1:504177/1‑300 (MQ=255)                                                  aaGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatc                                                                                                                                                                                                                                              <  1:606530/298‑1 (MQ=255)                                                                ccGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaaaa                                                                                                                                                                                                                              >  1:242808/1‑300 (MQ=255)                                                                      gCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCGCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAaactaact                                                                                                                                                                                                                        <  2:491203/300‑1 (MQ=255)                                                                                             aCTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCtggt                                                                                                                                                                                                 >  2:869646/1‑299 (MQ=255)                                                                                               tctGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCtggtgg                                                                                                                                                                                               <  1:512622/299‑1 (MQ=255)                                                                                                  ggtccgcAAGCGGCAACCGTGGTTCAAAGCGTAGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTT‑‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGcc                                                                                                                                                                                          <  2:202152/294‑1 (MQ=255)                                                                                                          aGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGNTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑Tgcgc                                                                                                                                                                                 >  2:692451/1‑300 (MQ=255)                                                                                                                 aCCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCt                                                                                                                                                                                >  2:590808/1‑294 (MQ=255)                                                                                                                 aCCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGc                                                                                                                                                                             >  1:358657/1‑297 (MQ=255)                                                                                                                        gTCAAAGCGTCGCTGGNACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACCCCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCNGCCGCTGGTGGTTGCCTCN‑TGCGCTGGCgggcgaa                                                                                                                                                                      >  1:729246/1‑295 (MQ=255)                                                                                                                        gTCAAAGCGTCGCTGGNACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCNGCCGCTGGTGGTTGCCT‑ANTGCGCTGGCgggcgaat                                                                                                                                                                     >  1:729254/1‑295 (MQ=255)                                                                                                                        gTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAatat                                                                                                                                                                    >  1:592362/1‑299 (MQ=255)                                                                                                                                      ggCACCGGGTTCGAAAGTCGTTTCTGACTATCTGGCAAAAGCGAAACTGACACCGTACCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAAcc                                                                                                                                                         <  2:248339/296‑1 (MQ=255)                                                                                                                                            gggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCNGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGa                                                                                                                                               >  2:280933/1‑300 (MQ=255)                                                                                                                                            gggTTCGAAAGTCGTTTCTGATTATCTAGCAAAAGCGAAACTGACACTGTATCTCGACGAACTGGGGTTTAACCTCGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAACGTANGTGCGGTGCTGTCCGGCAACCGTAGATTTGAAGGCCGTATCCATCCGCTGGTTAAAACTCACTGGCTGGCCTCGCT‑GCCGCTGGCGGTTGCCT‑A‑TGCGCTGACGGGAAat                                                                                                                                                                      >  2:281469/1‑277 (MQ=255)                                                                                                                                              gTTCGAAAGTCGTTTCTNATGATCTGGCAAAAGCGANACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATNAACCTGGCTTCTGAGc                                                                                                                                             <  1:742588/300‑1 (MQ=255)                                                                                                                                              gTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACATCGTATCTCGACGAACTGTGGTTTAACCTTTTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGc                                                                                                                                             <  2:802385/300‑1 (MQ=255)                                                                                                                                              gTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTTGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGc                                                                                                                                             <  1:310130/300‑1 (MQ=255)                                                                                                                                              gTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAACCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAATTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCATCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGc                                                                                                                                             >  2:236000/1‑300 (MQ=255)                                                                                                                                              gTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGc                                                                                                                                             <  1:512611/300‑1 (MQ=255)                                                                                                                                                     aGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTAGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCgg                                                                                                                                      <  1:213642/300‑1 (MQ=255)                                                                                                                                                              tGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATc                                                                                                                              >  2:560321/1‑299 (MQ=255)                                                                                                                                                              tGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATc                                                                                                                              <  1:527369/299‑1 (MQ=255)                                                                                                                                                                                 acaCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTAt                                                                                                          >  2:782067/3‑300 (MQ=255)                                                                                                                                                                                  aaCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATc                                                                                                         <  1:720134/300‑1 (MQ=255)                                                                                                                                                                                  aaCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATc                                                                                                         >  2:822501/1‑300 (MQ=255)                                                                                                                                                                                   aCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTTTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCt                                                                                                        <  1:51258/300‑1 (MQ=255)                                                                                                                                                                                   aCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCt                                                                                                        <  1:51324/300‑1 (MQ=255)                                                                                                                                                                                         actgTATCTCGACGAACTTGGGTTTAACCTCGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGTCCGCTGCCCGATCCTATCGAAACGGCAAGCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTGTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAg                                                                                                   <  2:75641/296‑1 (MQ=255)                                                                                                                                                                                          ccGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGat                                                                                                 >  2:26375/1‑300 (MQ=255)                                                                                                                                                                                          ccGTATCTCAACGAACTGGGGTTTAGCCTTGTGGGATCCGGTTG‑TACCACCTGTCTTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAATTTGAATGCCGTATCCATCCGCCGGTTAAAACTAACTGGCTGGCCTCTCC‑GCCGCTGATTGTTGCCT‑C‑Tgc                                                                                                                                                                                   >  2:397955/1‑218 (MQ=255)                                                                                                                                                                                                        acttgggTTTAACCTTGTTGGATACGGTTGCTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGGGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCgg                                                                                    <  2:729254/296‑1 (MQ=255)                                                                                                                                                                                                          tGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcac                                                                                 <  1:653792/300‑1 (MQ=255)                                                                                                                                                                                                              gTTTAACCTTGTGGTATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGa                                                                             >  1:803579/1‑300 (MQ=255)                                                                                                                                                                                                               tttAACCTTTTGGGATACGGTTGGTACCACCTGTATTGGTAACTCTGGGCCGCTACCCGATCCTAGAGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTTAAAGATATCTGGCCATCgg                                                                                    <  2:729246/293‑1 (MQ=255)                                                                                                                                                                                                                      ttgttgGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGcc                                                                      <  2:583672/299‑1 (MQ=255)                                                                                                                                                                                                                           ggATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCNGCAACCGTAACTTTGAAGGCCGTATCCATCCGNTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGc                                                                 >  2:651675/1‑299 (MQ=255)                                                                                                                                                                                                                           ggATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGc                                                                 >  1:3958/1‑299 (MQ=255)                                                                                                                                                                                                                                cGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGa                                                           >  1:514824/1‑300 (MQ=255)                                                                                                                                                                                                                                cGGTTG‑TACCAACTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGa                                                           >  2:127723/1‑300 (MQ=255)                                                                                                                                                                                                                                  gTTG‑TATCACCTGTATTGGTAACTCTTGGCCGCTGCCCGATCCTATTGAAACGGCAATCAAAAAATGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGCATCCCTCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGTTGGCGGTAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAAc                                                         <  1:139079/300‑1 (MQ=255)                                                                                                                                                                                                                                  gTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAAc                                                         >  2:797015/1‑300 (MQ=255)                                                                                                                                                                                                                                  gTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCAGTGCGGTAGAAc                                                         >  2:216585/1‑300 (MQ=255)                                                                                                                                                                                                                                  gTTG‑TACCACCGGTATTGGTAATTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAAc                                                         <  1:140557/300‑1 (MQ=255)                                                                                                                                                                                                                                  gTTG‑TACCAACTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTa                                                             >  2:245280/1‑296 (MQ=255)                                                                                                                                                                                                                                    tG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACaa                                                       <  2:571853/300‑1 (MQ=255)                                                                                                                                                                                                                                     g‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACTGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAg                                                      <  1:671146/300‑1 (MQ=255)                                                                                                                                                                                                                                        accaccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGtc                                                    <  1:127723/300‑1 (MQ=255)                                                                                                                                                                                                                                          caccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGtctc                                                  >  1:8508/1‑300 (MQ=255)                                                                                                                                                                                                                                              tGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCcac                                               <  1:874375/299‑1 (MQ=255)                                                                                                                                                                                                                                               gTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCcaca                                              >  1:503207/1‑299 (MQ=255)                                                                                                                                                                                                                                               gTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCcaca                                              >  2:846007/1‑299 (MQ=255)                                                                                                                                                                                                                                                        aCTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCACAGAAATGTTcc                                    >  1:828255/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCACAGAAATGTTCCGCAAagag                            <  1:61952/298‑1 (MQ=255)                                                                                                                                                                                                                                                                     gCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGANGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCACAGAAATGTTCCGCAAAGAGTACGc                       <  1:199374/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     gCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGTGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCACAGAAATGTTCCGCAAAGAGTACGc                       <  2:763220/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCNCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCACAGAAATGTTCCGCAAAGAGTACGCAGa                    <  2:695668/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         gATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCACAGAAATGTTCCGCAAAGAGTACGCa                      <  1:130560/297‑1 (MQ=255)                                                                                                                                                                                                                                                                          aTCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCACAGAAATGTTCCGCAAAGAGTACGCAGaa                   >  1:876885/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                   aaaCGGCAATCAAAGAAGGCGATTTGACCGTCGGTGCGGTGCTGTCCTGCAACCGTAACTTTGAAGGCCGTATCCATTCGCTGTGTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGTTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTCATc                                                                                                         <  2:124333/204‑1 (MQ=255)                                                                                                                                                                                                                                                                                          aaTCAAAAAAGGCGATTTAACCTTCGGTGCGGTGCTGTCCTGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCACATAAATGTTCCGCAAAGAGTACGCAGAAGTTTTTGAAGGCAcagc  <  2:66004/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                     GCGCTGTGGTCATTGCTGCGATAACCTCGTGCACCAACACCTCTAACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTG‑TACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCC‑GCCGCTGGTGGTTGCCT‑A‑TGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCACAGAAATGTTCCGCAAAGAGTACGCAGAAGTTTTTGAAGGCACAGC  >  NC_000913/1337104‑1337683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑