Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012971	862,724	T→C	S22P (TCT→CCT)	yliI →	aldose sugar dehydrogenase YliI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012971	862,724	0	T	C	98.9%	331.8 / NA	89	S22P (TCT→CCT)	yliI	aldose sugar dehydrogenase YliI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (42/46);  minor base A (1/0);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCACCATGATTGCCCGACTGTTATTACCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTTCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGT  >  NC_012971/862431‑863014                                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                    gggCACCATGATTGCCCGACTGTTATTACCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCaa                                                                                                                                                                                                                                                                                               <  1:299202/300‑1 (MQ=255) gggCACCATGATTGCCCGACTGTTATTACCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCGTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCaa                                                                                                                                                                                                                                                                                               <  1:699613/300‑1 (MQ=255)     aCCATGATTGCCCGACTGTTATTACCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGTAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACgg                                                                                                                                                                                                                                                                                            <  2:530809/299‑1 (MQ=255)           aTTGCCCGACTGTTATTACCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGTAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGt                                                                                                                                                                                                                                                                                     <  1:713839/300‑1 (MQ=255)                  gACTGTTATTACCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGTAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAg                                                                                                                                                                                                                                                                                <  1:287078/298‑1 (MQ=255)                    gggTTATTAACGTCAACGCAGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTAAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGAC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCACCCTTACTGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTACGCTCCCTGGGCGGCTCCTGCAATGGTAAATGTCGAAGtgct                                                                                                                                                                                                                                                                            >  2:222957/3‑300 (MQ=255)                    cTGTTATTCCCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTACCCCTTCATGTTTAAGTTTTTGTAACAATGAATTTGTTATCTACTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTCGTGCCCATTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGtgct                                                                                                                                                                                                                                                                            >  2:223306/1‑300 (MQ=255)                    cTGTTATTACCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTACTACACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCACCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGtgct                                                                                                                                                                                                                                                                            >  2:222917/1‑300 (MQ=255)                      gTTATTACCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGtgctgc                                                                                                                                                                                                                                                                          >  1:756917/1‑300 (MQ=255)                       ttattaCCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑CCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGTGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCa                                                                                                                                                                                                                                                                         >  2:60173/1‑300 (MQ=255)                        tattaCCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCaa                                                                                                                                                                                                                                                                        <  2:732977/300‑1 (MQ=255)                              cGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACaaa                                                                                                                                                                                                                                                                  >  1:553484/1‑300 (MQ=255)                                tCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGNCTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGNTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACt                                                                                                                                                                                                                                                                <  2:606757/300‑1 (MQ=255)                                          tAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATccc                                                                                                                                                                                                                                                      <  2:776506/300‑1 (MQ=255)                                               ttGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGTAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGgc                                                                                                                                                                                                                                                 <  1:221598/300‑1 (MQ=255)                                               ttGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGgc                                                                                                                                                                                                                                                 <  1:285530/300‑1 (MQ=255)                                               ttGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTGAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGgc                                                                                                                                                                                                                                                 <  1:91451/300‑1 (MQ=255)                                                ggAAAATTGGCTGATGAGCTTAACGCTGAGTATCCAGAGCCGGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAATGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTNTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCGCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGgc                                                                                                                                                                                                                                                 <  2:247108/298‑1 (MQ=255)                                                 gAAAATTGTCTGATGAGTTTAATGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAATGTGGA‑‑G‑‑AAAGCG‑‑CCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACGGACTACGCTTTAAGCCAG‑AGTTAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGgcgc                                                                                                                                                                                                                                               <  2:329343/300‑1 (MQ=255)                                                 gAAAATTGTCGGATGAGCTTAACGCTGAGTATCCAGAGCCTGCATAAGCACGCCATGCTTTAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCGTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGgcgc                                                                                                                                                                                                                                               <  2:329157/300‑1 (MQ=255)                                                                 gCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGa                                                                                                                                                                                                                               >  1:35940/1‑300 (MQ=255)                                                                 gCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGa                                                                                                                                                                                                                               >  2:674672/1‑300 (MQ=255)                                                                 gCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTACCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGa                                                                                                                                                                                                                               >  1:35436/1‑300 (MQ=255)                                                                            aGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTNCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTa                                                                                                                                                                                                                    >  1:180574/1‑300 (MQ=255)                                                                            aGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTACCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTNCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCATGGGCGCTGGCCTTTTTACCCGACAATCACGGTa                                                                                                                                                                                                                    >  1:179871/1‑300 (MQ=255)                                                                              tATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATg                                                                                                                                                                                                                  <  1:641933/300‑1 (MQ=255)                                                                                tCCAGAGCCTGCATAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑tt                                                                                                                                                                                                               <  2:129829/300‑1 (MQ=255)                                                                                tCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑tt                                                                                                                                                                                                               <  1:755322/300‑1 (MQ=255)                                                                                   agagCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTaa                                                                                                                                                                                                             <  1:390219/299‑1 (MQ=255)                                                                                    gagCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGTGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGTAGGCGTTATGCATCGACAATTCTTNTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCNCTCTGGTCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATc                                                                                                                                                                                                           <  2:618009/300‑1 (MQ=255)                                                                                    gagCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATTTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATc                                                                                                                                                                                                           <  2:279227/300‑1 (MQ=255)                                                                                    gagCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATc                                                                                                                                                                                                           <  1:633871/300‑1 (MQ=255)                                                                                    gagCCTACAGAAGCACGCCATGCGTGAGAAGGCC‑‑GGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑CCTCCCCCTTCATGTTTAAGTTTTTGGAAAAATGAATTTGTTATCTCCTCCACTAACTACGCTTTAAGCCAG‑AGTCAATCCGTAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATc                                                                                                                                                                                                           <  2:54653/300‑1 (MQ=255)                                                                                    gagCCGGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATc                                                                                                                                                                                                           <  1:633881/300‑1 (MQ=255)                                                                                        cTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCATGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCActc                                                                                                                                                                                                       >  1:733276/1‑300 (MQ=255)                                                                                        cTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCACTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCActc                                                                                                                                                                                                       >  1:733723/1‑300 (MQ=255)                                                                                        cTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCACTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTACACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCATGGGCGCTGGCCTTTTTAACCGACAATCACGGTATG‑TTAATCActc                                                                                                                                                                                                       >  2:794605/1‑300 (MQ=255)                                                                                             gAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTgcgc                                                                                                                                                                                                  <  1:59127/300‑1 (MQ=255)                                                                                                  aCGCCATGCTTGATAATGCC‑‑TGAAAACTAAGGTGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATGTGTTATCGCCTCCACTGACTACGCTTTAAGCCAT‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTCTCTTCCGCTCTCGGGGCGGCTCCTGCAACGGTAAATTTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATc                                                                                                                                                                                                           <  2:277708/286‑1 (MQ=255)                                                                                                     atgcgCTTTAGAAGGCCNGTGAAAACTAAGGGGGAGNG‑‑AAAGCGGCTCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAGGAGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATNCTTAATCACTCTgcgc                                                                                                                                                                                                  <  1:670796/296‑1 (MQ=255)                                                                                                       gcgCTTGAGAAGGCCNGTGAAAACTAAGGGGGA‑‑GNTAAAGCGGCTCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAGGAGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATNCTTAATCACTCTgcgc                                                                                                                                                                                                  <  1:670789/296‑1 (MQ=255)                                                                                                       aTGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTACCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTACTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCATGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGt                                                                                                                                                                                        >  2:114731/1‑300 (MQ=255)                                                                                                         gCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCAGACAATCACGGTATG‑TTAATCACTCTACGCGGCGGCGAGtt                                                                                                                                                                                       >  2:25338/1‑299 (MQ=255)                                                                                                                  ggCC‑‑GGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTgg                                                                                                                                                                             <  2:807313/300‑1 (MQ=255)                                                                                                                              cTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTACCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGaa                                                                                                                                                                   >  2:766118/1‑300 (MQ=255)                                                                                                                                          g‑‑aAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCATGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCAATGGCAAGCAGGAAAAGGATTATc                                                                                                                                                         >  2:562751/1‑300 (MQ=255)                                                                                                                                          g‑‑aAAGCG‑‑TCTACCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTACGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATc                                                                                                                                                         >  2:562746/1‑300 (MQ=255)                                                                                                                                                          cccTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACANTCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTc                                                                                                                                             >  1:589072/1‑300 (MQ=255)                                                                                                                                                                                            gTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGNCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGggc                                                                                                           <  1:155527/300‑1 (MQ=255)                                                                                                                                                                                            gTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGAAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGggc                                                                                                           <  1:530730/300‑1 (MQ=255)                                                                                                                                                                                            gTTACCTCCTCCACTGACTACGCTTGAAGCCGG‑AGTCAATCCTTAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTTGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGggc                                                                                                           <  2:320077/300‑1 (MQ=255)                                                                                                                                                                                                 ctcctcCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGAGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCct                                                                                                      >  2:335877/1‑300 (MQ=255)                                                                                                                                                                                                 ctcctcCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTATTTCTTACGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCACTGGGCGTTGGCCTTTTTACCCGACAATCGCGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTACCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGTGCGGCct                                                                                                      >  2:335802/1‑300 (MQ=255)                                                                                                                                                                                                   cctccACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCNAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCctgc                                                                                                    >  2:584074/1‑300 (MQ=255)                                                                                                                                                                                                   cctccACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTNACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCctgc                                                                                                    >  1:177394/1‑300 (MQ=255)                                                                                                                                                                                                   cctccACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCctgc                                                                                                    >  2:527664/1‑300 (MQ=255)                                                                                                                                                                                                    ctccACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCATGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCctgct                                                                                                   >  2:645887/1‑300 (MQ=255)                                                                                                                                                                                                     tccACTGACTACGCTTTAAGCCAG‑AGTCAATCCTGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTNATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCct                                                                                                      <  1:164313/296‑1 (MQ=255)                                                                                                                                                                                                     tccACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCctgctg                                                                                                  <  1:554589/300‑1 (MQ=255)                                                                                                                                                                                                                  cTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCttttt                                                                                                                                                                                                                                     <  1:628419/157‑1 (MQ=255)                                                                                                                                                                                                                  cTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCttttt                                                                                                                                                                                                                                     >  2:628419/1‑157 (MQ=255)                                                                                                                                                                                                                  cTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTa                                                                                      >  1:265409/1‑299 (MQ=255)                                                                                                                                                                                                                    ttAAGCCAG‑AGTCAATCCTTAGGCGTTATGCATCGACAGTTCTTTTTTCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCGGCAATACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAGTCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATTTGCGCCGCTTTCCGGGGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAgc                                                                                    <  1:390951/299‑1 (MQ=255)                                                                                                                                                                                                                    ttAAGCCAG‑AGTCAATCCGTAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAgcg                                                                                   <  1:608769/300‑1 (MQ=255)                                                                                                                                                                                                                       aGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTCTTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGCAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCt                                                                                >  2:500674/1‑300 (MQ=255)                                                                                                                                                                                                                       aGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCt                                                                                >  1:2335/1‑300 (MQ=255)                                                                                                                                                                                                                       aGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCATGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCCCTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCATGCTGGACGTGGTTTTAGCGCCt                                                                                >  2:500143/1‑300 (MQ=255)                                                                                                                                                                                                                                 cAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTTCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCt                                                                       >  1:185475/1‑300 (MQ=255)                                                                                                                                                                                                                                  aaTCCGTAGGCGTTATGCATCGACAATTCTTTTTACTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTc                                                                      <  1:720165/300‑1 (MQ=255)                                                                                                                                                                                                                                   aTCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCa                                                                     >  2:468247/1‑300 (MQ=255)                                                                                                                                                                                                                                          ggCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTCTTTGTCTTTCTTCAGCTCTCTGGGCGGCTACTGCAACGGTGAATGTCGACGTGCTGCAAGACGAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCAGACAATCACGGTATG‑TTAATCACTCTGCGCGGTGGCGAGTTGCATAAATGGCAAGCGGGAAAAAGCCTATCTGCGCAGCTCCACGGAGTTCTTGAAGCTTGGGCTCACGGGCATGGAGGCCTGCTGGACGTGTTTTTAGCGCCTTCTTTTGCTCAGTCTcgc                                                              >  2:441587/1‑300 (MQ=255)                                                                                                                                                                                                                                          ggCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGAACATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCATGATTTTGCTCAGtctc                                                                >  2:444670/1‑298 (MQ=255)                                                                                                                                                                                                                                             gTTATGCATCGACAATTCTTTTTCCTTGTTCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTcgccg                                                            <  2:687373/299‑1 (MQ=255)                                                                                                                                                                                                                                             gTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTcgccgc                                                           >  2:738386/1‑300 (MQ=255)                                                                                                                                                                                                                                                    aTCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGt                                                    >  1:508433/1‑300 (MQ=255)                                                                                                                                                                                                                                                          aaTTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCTGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGtt                                              <  2:748551/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aaTTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGtt                                              <  1:546398/300‑1 (MQ=255)                                                                                                                                                                                                                                                               tttttCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTc                                          <  2:127782/299‑1 (MQ=255)                                                                                                                                                                                                                                                                          gCCCATTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCAGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCATGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCga                              >  2:40044/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             ccTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCAAGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTACTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAGGCTGGCgct                             >  2:341462/1‑296 (MQ=255)                                                                                                                                                                                                                                                                               ttATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCgatgat                          >  2:27303/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                         ttcttcCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGcc                 >  2:676782/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                            ttcCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAAc            <  1:16694/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   ctctGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGTTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCAGGAActg          >  2:801242/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                   ctctGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCNCTCTGCGCGGCGGCGAGTTGCGTCACTGGCAANCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAActg          >  2:619074/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                      ttggcggcTCCTGCAACGGTAAATGTCTAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGTAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGt  <  2:35436/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      tGGGCGGCTCCTTCAACGGTAAATTTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTGACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTTGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCTGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTTGCGATGATGGCAAAGCCGGAACTGCTGTGGGt  <  1:222957/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      tGGGCGGCTCCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGt  <  1:222917/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         gcggcTCCTGCAGCGGTAAATTTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCTGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGt  <  2:35940/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                    GGGCACCATGATTGCCCGACTGTTATTACCGTCAACGCCGATAAGCTTGAAAATTGTCTGATGAGCTTAACGCTGAGTATCCAGAGCCTGCAGAAGCACGCCATGCTTGAGAAGGCC‑‑TGAAAACTAAGGGGGA‑‑G‑‑AAAGCG‑‑TCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAG‑AGTCAATCCGGAGGCGTTATGCATCGACAATTCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTTCTGCAACGGTAAATGTCGAAGTGCTGCAAGACAAACTTGACCATCCCTGGGCGCTGGCCTTTTTACCCGACAATCACGGTATG‑TTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGT  >  NC_012971/862431‑863014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑