Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012971	767,889	T→C	M60T (ATG→ACG)	ybcW →	protein YbcW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012971	767,889	0	T	C	100.0%	224.8 / NA	60	M60T (ATG→ACG)	ybcW	protein YbcW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/32);  total (28/32)

AATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACC  >  NC_012971/767724‑768179                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                   aatacactgatgccccctcccaggagcaggttcgtgtaaaaactatgcttaatagcaccattTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgc                                                                                                                                                              <  1:23631‑M1/270‑85 (MQ=255)       ctgctgccccctcccaggagcaggttcgtgtaaaaactatgcttaatagcACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatac                                                                                                                                                                                         >  2:313320‑M1/25‑210 (MQ=255)       ctgatgccccctcccaggagcaggttcgtgtaaaaactatgcttaatagcACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatac                                                                                                                                                                                         <  1:313320‑M1/243‑58 (MQ=255)                cctcccaggagcaggttcgtgtaaaaactatgCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtga                                                                                                                                               >  1:65392‑M1/16‑201 (MQ=255)                cctaccaggagcaggttcgtgtaaaaactatgCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTATGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtga                                                                                                                                               >  1:65418‑M1/16‑201 (MQ=255)                  tcccaggagcaggttcgtgtaaaaactaTGCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaat                                                                                                                                             >  2:465998‑M1/14‑199 (MQ=255)                   cccaggagcaggttcgtgtaaaaactATGCTTAATAGCACCATTTCGATGGGTTACCAGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTCACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctagtatttaggtgaatt                                                                                                                                            >  2:13822‑M1/13‑198 (MQ=255)                     caggagcaggttcgtgtaaaaaCTATGCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattga                                                                                                                                          >  1:604525‑M1/11‑196 (MQ=255)                      aggagtaggttcgtgtaaaaATTATGCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtattaatac                                                                                                                                                 <  2:182119‑M1/283‑98 (MQ=255)                        gagcaggttcgtgtaaAAACTATGCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcc                                                                                                                                       >  1:506272‑M1/8‑193 (MQ=255)                        gagcaggttcgtgtaaAAACTATGCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatc                                                                                                                                        <  2:13043‑M1/292‑107 (MQ=255)                                      aaaaacTATGCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatat                                                                                                                         <  1:304887‑M1/300‑122 (MQ=255)                                      aaaaacTATGCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatat                                                                                                                         <  2:72176‑M1/300‑122 (MQ=255)                                          actatgcTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggac                                                                                                                     <  1:254657‑M1/298‑126 (MQ=255)                                            tatGCTTAATAGCACCATTTCGATTGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAATTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaattgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatg                                                                                                                        <  2:347316‑M1/295‑123 (MQ=255)                                            tatGCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacac                                                                                                                   <  1:552435‑M1/300‑128 (MQ=255)                                            tatGCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCNAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacac                                                                                                                   <  2:580525‑M1/300‑128 (MQ=255)                                               gCTTAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCCCGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttaccaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattccactacagcaatgcctcgtacttaggtgaattgatcctactcacgtagta                                                                                                                          >  2:7519‑M1/1‑170 (MQ=255)                                                  tAATAGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctccccacgtaatatggacacaggcct                                                                                                             >  2:558333‑M1/1‑167 (MQ=255)                                                      aGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGTAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagc                                                                                                         <  1:194524‑M1/300‑138 (MQ=255)                                                      aGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaattgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagc                                                                                                         <  1:194923‑M1/300‑138 (MQ=255)                                                      aGCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagc                                                                                                         <  2:794728‑M1/300‑138 (MQ=255)                                                       gCACCATTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcg                                                                                                        <  2:567229‑M1/300‑139 (MQ=255)                                                            gtttcGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTATGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAAATGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcactgcaattcaactacagccatgcctagtatttaggtgaattgatcctacccacgtcatatggacaccggcctaagcgaggtt                                                                                                   >  1:263121‑M1/2‑157 (MQ=255)                                                            aTTTCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatgcagcaatgcaattcaactaaagcaatgaatagtatttaggtgaattgatcctaaccacgtaatatggatacagacctaagtgaggtt                                                                                                   >  2:21721‑M1/1‑157 (MQ=255)                                                             tttCGATGGGTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGCAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacacacgtaatatggacacaggcctaagcgaggtt                                                                                                   >  2:432040‑M1/1‑156 (MQ=255)                                                                    gggttACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctagtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgtttt                                                                                           >  2:692873‑M1/3‑149 (MQ=255)                                                                      gTTACCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttca                                                                                         >  1:45770‑M1/1‑147 (MQ=255)                                                                         aCCCGGATGTTGTAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaat                                                                                      <  2:804905‑M1/300‑157 (MQ=255)                                                                                  ttgtAATTGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactncagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccgga                                                                             >  2:634531‑M1/1‑135 (MQ=255)                                                                                         tGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACNAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggc                                                                      <  1:580659‑M1/300‑173 (MQ=255)                                                                                         tGCATGTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggc                                                                      >  1:539792‑M1/1‑128 (MQ=255)                                                                                              gTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATNACCATAACTGCTAACCATTCtgaccatttcacnagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgcca                                                                 >  2:605588‑M1/1‑123 (MQ=255)                                                                                              gTATAGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgcca                                                                 >  1:467675‑M1/1‑123 (MQ=255)                                                                                              gTATAGAACATCAAGTGTCTATGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccaagtaatgtggacacaggcctaagtgaggtttttgttttccaattgttccggactgaggccgcca                                                                 >  2:223532‑M1/1‑123 (MQ=255)                                                                                              gTATAGAACATCAAGTGTCTATGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctaaccacgtaatatggacacaggcctaagcggggttcttgttttcaaattgttccggactgaggccgcca                                                                 >  2:109919‑M1/1‑123 (MQ=255)                                                                                               tagagaaCATCAAGTGTCTCTGGAAGCATTCAGGGCAACTGAGGCCGCGTTAGTAAAGCACGAGAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcanaagtgacagagtcaatatcgtattctgtcactggcaggctaatacagcaatgcaatgcaactacagcaatgcctcgtatgtaggtgaatgggtcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgacgccaccac                                                                <  2:263121‑M1/297‑179 (MQ=255)                                                                                                 taGAACATCAAGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacac                                                              <  1:303931‑M1/300‑181 (MQ=255)                                                                                                     acatcaagtgtctctggaAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgtcacaccaac                                                          <  2:748714‑M1/293‑185 (MQ=255)                                                                                                      catcaagtgtctctggAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaact                                                         >  2:351512‑M1/7‑115 (MQ=255)                                                                                                       atcaagtgtctctgTAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactg                                                        <  1:353709‑M1/295‑187 (MQ=255)                                                                                                       atcaagtgtctctgGAAGCATTCAGTGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactg                                                        <  2:350354‑M1/295‑187 (MQ=255)                                                                                                          aagtgtctCTGTAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgc                                                     <  1:388047‑M1/298‑190 (MQ=255)                                                                                                           agtgtcTCTGGAAGCATTCAGGGCAATTGAGGCCGTTTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaattgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgcc                                                    <  2:320864‑M1/299‑191 (MQ=255)                                                                                                           agtgtcTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaacgacagcaatgcctcgtatttaggtgaattgatcctacccacttaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgcc                                                    <  2:20081‑M1/299‑191 (MQ=255)                                                                                                              gtCTCTGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctagtatttaggtgaattgatcctacccacgtaatatggccacaggcctaagcgaggttcttgtcttcaaattgttccggactgaggccgccacaccaactgtgccgcc                                                 >  2:654902‑M1/1‑107 (MQ=255)                                                                                                                ctctGGAAGCATTCAGGGCAATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgc                                               >  1:126053‑M1/1‑105 (MQ=255)                                                                                                                              atggcaaGTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatc                                 <  1:764687‑M1/297‑210 (MQ=255)                                                                                                                              agggcaaTTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatc                                 <  1:764681‑M1/297‑210 (MQ=255)                                                                                                                               gggcaATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctagtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccaaaccaactgtgccgccgccaccgattgtaacc                                 >  2:489135‑M1/3‑90 (MQ=255)                                                                                                                               gggcaATTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgccaggctaatacagcaatgcaattcaactacagcaatgcctagtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgcagccaccgattgtaatca                                >  2:319209‑M1/3‑90 (MQ=255)                                                                                                                                   aaTTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaattgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcaca                              <  2:297970‑M1/298‑213 (MQ=255)                                                                                                                                    aTTGAGGCCGCGTTAGTAAAGCACGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcacattc                           <  1:394485‑M1/300‑216 (MQ=255)                                                                                                                                                 tagtaaagcacgATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcacattcgatataattaaac              <  1:498956‑M1/295‑229 (MQ=255)                                                                                                                                                  agtaaagcacGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcacattcgatataattaaaca             >  1:129672‑M1/5‑71 (MQ=255)                                                                                                                                                  agtaaagcacGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaaggcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcacattcgatataattaaac              <  2:727630‑M1/295‑229 (MQ=255)                                                                                                                                                   gtaaagcaCGATAAGAATACGAAGGATTATTCCTTGGTGGTTGACTGATCACTAGCACTGCTAACCATTCtgaccatttcacaattgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggaccgagaccgccacaccaactgtgccgccaccaccaattgtaatcacattcgatataattacacac            <  2:513042‑M1/297‑231 (MQ=255)                                                                                                                                                   gtaaagcaCGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTGACCATTCtgaccatttcacaagtgacagagtcaatatcggattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctagtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcacattcgatataattaaacac            >  2:704316‑M1/4‑70 (MQ=255)                                                                                                                                                   gtaaagcaCGATAAGAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcacattcgatataattaaacac            <  1:788631‑M1/297‑231 (MQ=255)                                                                                                                                                             ataagaataCGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAACCATTCtgaccatttcacaagtgacagagtcaatatcgtattctgtcactgtcaggctaatacagcaatgcaattcaactacagcaatgcctcgtatttaggtgaattgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcacattcgatataattaaacaccgttgcccgc  >  2:795923‑M1/1‑60 (MQ=255)                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                   AATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACC  >  NC_012971/767724‑768179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑