BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012971	762,918	T→C	T186T (ACT→ACC)	ECD_RS03820 →	replication protein P

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012971	762,918	0	T	C	99.1%	395.4 / NA	105	T186T (ACT→ACC)	ECD_RS03820	replication protein P
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (52/52); total (53/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GTAGCCCGTCGGCAGAATCGACCATTTCTGCCATCACCCGGGCAGTTTGTTGCATGGTGCCGGGAAGAAGCATCCGTTACCGCCGGACTGCCAAACGTCAGCGAGCTGGTTGATATGGTTTACGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCC‑AT‑ATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAACCAGCATAAATCAGGCCGCGGCTAAAATGGCACGGGCAGGTCTTCTGGTTATCGAAGGTAAGGT  >  NC_012971/762631‑763213

gtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAAc                                                                                                                                                                                                                                                                                             <  1:116086‑M2/70‑1 (MQ=255)

     gaatctgacctcacttgcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCgtgg                                                                                                                                                                                                                                                                                        <  2:759339‑M2/75‑1 (MQ=255)

      aatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCgtggt                                                                                                                                                                                                                                                                                       >  1:432823‑M2/225‑300 (MQ=255)

      aatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtaagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCgtggt                                                                                                                                                                                                                                                                                       >  2:435108‑M2/225‑300 (MQ=255)

        tctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccanaggcatatcccattgcttactggttggttaccnacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCgtggtg                                                                                                                                                                                                                                                                                      <  2:600089‑M2/77‑1 (MQ=255)

               tcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacaccccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGa                                                                                                                                                                                                                                                                                <  2:775989‑M2/83‑1 (MQ=255)

                 actggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGcgatacc                                                                                                                                                                                                                                                                            <  1:521180‑M2/87‑4 (MQ=255)

                          ttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGt                                                                                                                                                                                                                                                                   >  1:400451‑M2/205‑300 (MQ=255)

                            gtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTTAACCAGTaa                                                                                                                                                                                                                                                                 >  1:211051‑M2/203‑300 (MQ=255)

                            gtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTaa                                                                                                                                                                                                                                                                 >  1:210564‑M2/203‑300 (MQ=255)

                                    tcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACtt                                                                                                                                                                                                                                                         >  2:128207‑M2/195‑300 (MQ=255)

                                           accatcctacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagttgctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacntgtaccagaacatgcgggccaaTGCGCTGACTNACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCa                                                                                                                                                                                                                                                  <  2:620607‑M2/113‑1 (MQ=255)

                                           accatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCa                                                                                                                                                                                                                                                  <  1:425996‑M2/113‑1 (MQ=255)

                                                   acagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGt                                                                                                                                                                                                                                          >  2:556104‑M2/180‑300 (MQ=255)

                                                       atgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggnaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAc                                                                                                                                                                                                                                      <  1:151924‑M2/125‑1 (MQ=255)

                                                               gcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAAt                                                                                                                                                                                                                              <  1:510150‑M2/133‑1 (MQ=255)

                                                               gcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTACTGTCATGGGCGGTAGACCTCTAAAt                                                                                                                                                                                                                              <  1:499127‑M2/133‑1 (MQ=255)

                                                                  ggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataancagaggcatatcccattgcttactggttggttnccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGt                                                                                                                                                                                                                           >  2:619734‑M2/165‑300 (MQ=255)

                                                                  ggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCCCTAAATCGt                                                                                                                                                                                                                           >  1:114713‑M2/165‑300 (MQ=255)

                                                                    taataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGAACGCGCGAATTAACCGTGGTGGGGCGATACCTGAACCAGTAAAACAACTTACTGTAATGGGCGGTAGACCTCTAAATCGt                                                                                                                                                                                                                           >  2:498462‑M2/163‑298 (MQ=255)

                                                                         acattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGcaca                                                                                                                                                                                                                      >  2:502828‑M2/158‑298 (MQ=255)

                                                                           attacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGct                                                                                                                                                                                                                  <  1:503247‑M2/145‑1 (MQ=255)

                                                                                 catcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGCCCTCTAAATCGTGCACAGGCTCTGGc                                                                                                                                                                                                             >  2:688920‑M2/150‑299 (MQ=255)

                                                                                 catcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCAGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGCACCAGTAAAACAACTTCCTGTCATGGGCGGTAGCCCTCTAAATCGTACACAGGCTCTGGc                                                                                                                                                                                                             >  2:451194‑M2/150‑299 (MQ=255)

                                                                                  atcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTAATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGa                                                                                                                                                                                                           >  2:363525‑M2/149‑300 (MQ=255)

                                                                                  atcccacactgctgaatttgttttcagtggctgcctttttcaaaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCCTGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGa                                                                                                                                                                                                           >  2:362699‑M2/149‑300 (MQ=255)

                                                                                  actctacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgttgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagagcatgcgtgccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGTCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTTGCGa                                                                                                                                                                                                           <  2:425913‑M2/152‑1 (MQ=255)

                                                                                       acactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGAt                                                                                                                                                                                                       <  1:280749‑M2/156‑1 (MQ=255)

                                                                                       acactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGAt                                                                                                                                                                                                       <  1:121701‑M2/156‑1 (MQ=255)

                                                                                          ctgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCa                                                                                                                                                                                                   >  1:512441‑M2/141‑300 (MQ=255)

                                                                                                  tttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGNACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCaa                                                                                                                                                                                           >  1:182546‑M2/133‑300 (MQ=255)

                                                                                                  tttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGAGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGNACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCaa                                                                                                                                                                                           >  1:182556‑M2/133‑300 (MQ=255)

                                                                                                           agtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGtt                                                                                                                                                                                  <  2:657760‑M2/177‑1 (MQ=255)

                                                                                                                 tgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGNAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACt                                                                                                                                                                            <  2:255498‑M2/183‑1 (MQ=255)

                                                                                                                   cctttttcacaaagggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAACTAAGTTCGGACTGa                                                                                                                                                                          >  2:147227‑M2/116‑300 (MQ=255)

                                                                                                                             aaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAg                                                                                                                                                                <  2:453755‑M2/195‑1 (MQ=255)

                                                                                                                                aggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAgtgt                                                                                                                                                             <  1:440772‑M2/198‑1 (MQ=255)

                                                                                                                                aggggaaatattgctggatggtggattcaaccagacataaccataggcatatcccattgcttacttgttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATGACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGTTGAGGCGATACCGGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAgtgt                                                                                                                                                             <  1:441103‑M2/198‑1 (MQ=255)

                                                                                                                                  ggtaaatattactgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTAt                                                                                                                                                           <  2:57076‑M2/200‑1 (MQ=255)

                                                                                                                                     aaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                                                                        <  1:722919‑M2/203‑1 (MQ=255)

                                                                                                                                     aaatattgctgtatggtggatgcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                                                                        <  2:755224‑M2/203‑1 (MQ=255)

                                                                                                                                      aatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactgtttggttaccaacttgtaccagaacatgctggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTTAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                                                                        <  2:225626‑M2/203‑1 (MQ=255)

                                                                                                                                       atattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACgg                                                                                                                                                      <  2:746545‑M2/205‑1 (MQ=255)

                                                                                                                                              tgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAaga                                                                                                                                               <  1:724414‑M2/212‑1 (MQ=255)

                                                                                                                                               gtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCCAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGNCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACt                                                                                                                                                                            >  2:249176‑M2/88‑270 (MQ=255)

                                                                                                                                                        attcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTcggactgaaaggatcaagtgtatctgtctcttatacacatctcgc                                                                                                                                     <  1:618788‑M2/222‑23 (MQ=255)

                                                                                                                                                            aaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCAtt                                                                                                                                 >  1:289748‑M2/75‑300 (MQ=255)

                                                                                                                                                            aaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCAtt                                                                                                                                 >  1:290232‑M2/75‑300 (MQ=255)

                                                                                                                                                                agacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTAcc                                                                                                                              <  1:298177‑M2/229‑1 (MQ=255)

                                                                                                                                                                agacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTAcc                                                                                                                              <  1:298553‑M2/229‑1 (MQ=255)

                                                                                                                                                                 gccataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACAGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTAATGGGCGGTAGAACTCCAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAagtatt                                                                                                                                     >  2:215272‑M2/70‑291 (MQ=255)

                                                                                                                                                                 gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                                                                             >  1:382984‑M2/70‑299 (MQ=255)

                                                                                                                                                                 gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTAATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTa                                                                                                                                >  2:656750‑M2/70‑296 (MQ=255)

                                                                                                                                                                 gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAAATTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                                                                             >  1:382985‑M2/70‑299 (MQ=255)

                                                                                                                                                                 gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGAACGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                                                                             >  1:383224‑M2/70‑299 (MQ=255)

                                                                                                                                                                 gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCNGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATNAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                                                                             >  2:600727‑M2/70‑299 (MQ=255)

                                                                                                                                                                   cataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATtacat                                                                                                                             >  1:635234‑M2/68‑295 (MQ=255)

                                                                                                                                                                   cataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                                                                             >  1:635204‑M2/68‑297 (MQ=255)

                                                                                                                                                                      aaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGAc                                                                                                                       >  1:800859‑M2/65‑300 (MQ=255)

                                                                                                                                                                             ggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCataa                                                                                                                 >  2:22004‑M2/58‑299 (MQ=255)

                                                                                                                                                                             ggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCata                                                                                                                  >  2:22035‑M2/58‑298 (MQ=255)

                                                                                                                                                                                atatcctattgcttactgtttggTTACCAACTTGTACCAGAACATGCGTGCCAATGCGCTGACTTACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTTAGGCGATACCTGAACCAGTAAAACAACTGCCGGTCATGGGCGTTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGc                                                                                                             <  1:183571/280‑1 (MQ=255)

                                                                                                                                                                                atatcccattgcttactggttggttaccaacttgtaccagaacatgcgtgccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGc                                                                                                             <  1:183141‑M2/246‑1 (MQ=255)

                                                                                                                                                                                          gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACTGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTgtgcac                                                                                                   <  2:57994‑M2/256‑3 (MQ=255)

                                                                                                                                                                                          gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTgtgcac                                                                                                   <  1:128207‑M2/256‑3 (MQ=255)

                                                                                                                                                                                          gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTgtgcac                                                                                                   <  1:25821‑M2/256‑3 (MQ=255)

                                                                                                                                                                                                tggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGTCGATACCTGAACCAGTAAAACAATTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGcttctgtgcacaggac                                                                                              <  2:364472‑M2/261‑8 (MQ=255)

                                                                                                                                                                                                tggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGcttctgtgcacaggac                                                                                              <  2:364539‑M2/261‑8 (MQ=255)

                                                                                                                                                                                                  gttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGGAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCATTAACTGGGGACGCATAAGAGCTTCTGTGCACAGGACGtt                                                                                           >  2:110562‑M2/37‑300 (MQ=255)

                                                                                                                                                                                                    tggttaccaacttgtaccagaacatgctggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTTTCATGGGCGGTAGACCTCTAAATCGTGCACAAGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGc                                                                                         <  1:339197‑M2/266‑1 (MQ=255)

                                                                                                                                                                                                    tggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGc                                                                                         <  1:339188‑M2/266‑1 (MQ=255)

                                                                                                                                                                                                      gTTACCAACTTGTACCAGAACATGCGGGCAAATGCGCTGACTGACGAGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAAACGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCCCAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAGGGCTCAAGTGTCTGACGGGCAAAGAGGCAATTATTCATTACTTGGGGACGCATAAGAGCttat                                                                                                         >  2:197291/1‑280 (MQ=255)

                                                                                                                                                                                                            aacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTATGCACAGGACGTTGCCGCGGTaa                                                                                 >  2:167335‑M2/27‑300 (MQ=255)

                                                                                                                                                                                                            aacttataccAGAACATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGTAAGGCTGCTGATGAACT‑GTCC‑TTTATGCATGCGCGGATTACCCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGTGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAATATCGCAGAAATCAAAGTTAAGTTCGGACTGAAAGGATCAAGTGTCTGACGGGCAAAGAGGCAATTATTCAt                                                                                                                                  >  2:167054/10‑251 (MQ=255)

                                                                                                                                                                                                               ttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTATAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAg                                                                              <  1:607859‑M2/277‑1 (MQ=255)

                                                                                                                                                                                                                  taccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAAcaggtg                                                                           <  1:772112‑M2/280‑3 (MQ=255)

                                                                                                                                                                                                                     cAGAACATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCaa                                                                        >  1:196786/1‑300 (MQ=255)

                                                                                                                                                                                                                       gAACATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAcc                                                                      <  1:627693/300‑2 (MQ=255)

                                                                                                                                                                                                                        aaCATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTTGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAccg                                                                     <  2:432823/300‑3 (MQ=255)

                                                                                                                                                                                                                        aaCATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCCAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAccg                                                                     <  2:553804/300‑3 (MQ=255)

                                                                                                                                                                                                                         aCATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAccgt                                                                    >  1:479506/1‑297 (MQ=255)

                                                                                                                                                                                                                             gcgtgCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAAcc                                                                <  2:693221/296‑1 (MQ=255)

                                                                                                                                                                                                                                    aaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATaa                                                         >  2:151965/1‑300 (MQ=255)

                                                                                                                                                                                                                                         gctgaCTGACGCGGAACTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACTTTTGTTAGGCGATACCTTAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATc                                                      <  2:211051/294‑1 (MQ=255)

                                                                                                                                                                                                                                                               gcgcaaggctgccgatgaactgtcctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGcgc                                 >  1:615550/28‑296 (MQ=255)

                                                                                                                                                                                                                                                               gcgcaaggctgccgatgaactgtcctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGAAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCATAGGACGTTGCCGCGGTAACAGGTGCAAACGTAAACAGCCTAAATCAGGCTGCGGCTAAAATGGc                                   >  2:453105/28‑296 (MQ=255)

                                                                                                                                                                                                                                                                        tgccgatgaactgtcctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAATAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaat                       <  2:726736/281‑4 (MQ=255)

                                                                                                                                                                                                                                                                         gccgatgaactgtcctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcc                     <  1:106839/283‑6 (MQ=255)

                                                                                                                                                                                                                                                                          ccgatgaactgtcctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcct                    >  2:757118/17‑294 (MQ=255)

                                                                                                                                                                                                                                                                             gtgaactgtcctgt‑atGACCGCGCGAATTAACCTTGGTTAGTCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGTTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACGGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcctggt                 <  2:321134/287‑10 (MQ=25)

                                                                                                                                                                                                                                                                              tgaactgtcctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcctggtc                <  1:556104/288‑11 (MQ=37)

                                                                                                                                                                                                                                                                               gaactgtcctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGTCTAAAATGTCGCGGGCAggaatcctggtc                >  1:239119/12‑289 (MQ=255)

                                                                                                                                                                                                                                                                                    gtcctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCCTAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAAAAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcctggtcgttgat          >  1:335595/7‑284 (MQ=37)

                                                                                                                                                                                                                                                                                    gtcctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcctggtcgttgat          >  1:115390/7‑284 (MQ=37)

                                                                                                                                                                                                                                                                                    gtcctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCAGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcctggtagttgat          >  2:33979/7‑284 (MQ=37)

                                                                                                                                                                                                                                                                                       ctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcctggtcgttgatggt       >  1:6758/4‑281 (MQ=37)

                                                                                                                                                                                                                                                                                       ctgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGATAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTAACTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCNGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcctggtcgttgatggt       >  1:151375/4‑281 (MQ=37)

                                                                                                                                                                                                                                                                                        tgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcctggtcgttgatggta      <  2:400451/298‑21 (MQ=37)

                                                                                                                                                                                                                                                                                        tgt‑atGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGAAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcctggtcgttgatggta      <  1:502828/298‑21 (MQ=37)

                                                                                                                                                                                                                                                                                             tGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATCAGGCTGCGGCTAAAATGGCGCGGGCAggaatcctggtcgttgatggtaaggt  <  1:790236/300‑25 (MQ=37)

GTAGCCCGTCGGCAGAATCGACCATTTCTGCCATCACCCGGGCAGTTTGTTGCATGGTGCCGGGAAGAAGCATCCGTTACCGCCGGACTGCCAAACGTCAGCGAGCTGGTTGATATGGTTTACGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCC‑AT‑ATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAACCAGCATAAATCAGGCCGCGGCTAAAATGGCACGGGCAGGTCTTCTGGTTATCGAAGGTAAGGT  >  NC_012971/762631‑763213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`