BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012971	762,897	A→C	A179A (GCA→GCC)	ECD_RS03820 →	replication protein P

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012971	762,897	0	A	C	98.9%	324.0 / NA	91	A179A (GCA→GCC)	ECD_RS03820	replication protein P
Reads supporting (aligned to +/- strand): ref base A (1/0); major base C (44/45); minor base T (1/0); total (46/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TGGAACAGGTTAACGCAGGAATGCGCGTAGCCCGTCGGCAGAATCGACCATTTCTGCCATCACCCGGGCAGTTTGTTGCATGGTGCCGGGAAGAAGCATCCGTTACCGCCGGACTGCCAAACGTCAGCGAGCTGGTTGATATGGTTTACGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCC‑AT‑ATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAACCAGCATAAATC  >  NC_012971/762605‑763161

ggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccccactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGAtgaac                                                                                                                                                                                                                                                                     >  1:726736‑M2/257‑298 (MQ=255)

 gttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaacagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGatgaact                                                                                                                                                                                                                                                                    >  2:472840‑M2/256‑297 (MQ=255)

    aataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATgaa                                                                                                                                                                                                                                                                      >  1:457599‑M2/253‑294 (MQ=255)

     ataaatgccagtcgtcctgccttgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcatgtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTgccgatgaactgtcc                                                                                                                                                                                                                                                                <  1:445906‑M2/49‑8 (MQ=255)

     ataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTgccgatgaactgtcc                                                                                                                                                                                                                                                                <  1:445891‑M2/49‑8 (MQ=255)

          tgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAaggctgccgatgaactgtcctgtat                                                                                                                                                                                                                                                           <  1:662581‑M2/54‑13 (MQ=255)

          atgccagtcgtccttccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcataggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaagggtaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAaggctgccgatgaactgtcctgtat                                                                                                                                                                                                                                                           <  1:305189‑M2/54‑13 (MQ=255)

           gccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatgntggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAaggctgccgatgaactgtcctgtat                                                                                                                                                                                                                                                           <  2:581004‑M2/54‑13 (MQ=255)

              agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtgtattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                        <  2:697769‑M2/57‑16 (MQ=255)

              agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                        <  2:414707‑M2/57‑16 (MQ=255)

              agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                        <  2:505382‑M2/57‑16 (MQ=255)

              agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                        <  1:750962‑M2/57‑16 (MQ=255)

                  gtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTacggcgcaaggctgccgatgaactgtcctgtatgaccgcgc                                                                                                                                                                                                                                                   >  2:25821‑M2/239‑280 (MQ=255)

                  gtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTacggcgcaaggctgccgatgaactgtcctgtatgaccgcgc                                                                                                                                                                                                                                                   >  1:553804‑M2/239‑280 (MQ=255)

                       gccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGgaattacggcgcaaggctgccgatgaactgtcctgtatgaccgcgcgaatt                                                                                                                                                                                                                                              >  2:106839‑M2/234‑275 (MQ=255)

                          gtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAAc                                                                                                                                                                                                                                         <  1:116086‑M2/70‑1 (MQ=255)

                               gaatctgacctcacttgcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCgtgg                                                                                                                                                                                                                                    <  2:759339‑M2/75‑1 (MQ=255)

                                aatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCgtggt                                                                                                                                                                                                                                   >  1:432823‑M2/225‑300 (MQ=255)

                                  tctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccanaggcatatcccattgcttactggttggttaccnacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCgtggtg                                                                                                                                                                                                                                  <  2:600089‑M2/77‑1 (MQ=255)

                                         tcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacaccccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGa                                                                                                                                                                                                                            <  2:775989‑M2/83‑1 (MQ=255)

                                           actggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGcgatacc                                                                                                                                                                                                                        <  1:521180‑M2/87‑4 (MQ=255)

                                                      gtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTTAACCAGTaa                                                                                                                                                                                                             >  1:211051‑M2/203‑300 (MQ=255)

                                                      gtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTaa                                                                                                                                                                                                             >  1:210564‑M2/203‑300 (MQ=255)

                                                      gtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcaaaaaaggggaaatattgctgtatggtggattcaaccagacataaacagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTaa                                                                                                                                                                                                             >  2:482091‑M2/203‑300 (MQ=255)

                                                              tcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACtt                                                                                                                                                                                                     >  2:128207‑M2/195‑300 (MQ=255)

                                                                     accatcctacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagttgctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacntgtaccagaacatgcgggccaaTGCGCTGACTNACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCa                                                                                                                                                                                              <  2:620607‑M2/113‑1 (MQ=255)

                                                                     accatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCa                                                                                                                                                                                              <  1:425996‑M2/113‑1 (MQ=255)

                                                                             acagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGt                                                                                                                                                                                      >  2:556104‑M2/180‑300 (MQ=255)

                                                                                 atgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggnaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAc                                                                                                                                                                                  <  1:151924‑M2/125‑1 (MQ=255)

                                                                                         gcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAAt                                                                                                                                                                          <  1:510150‑M2/133‑1 (MQ=255)

                                                                                         gcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTACTGTCATGGGCGGTAGACCTCTAAAt                                                                                                                                                                          <  1:499127‑M2/133‑1 (MQ=255)

                                                                                            ggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataancagaggcatatcccattgcttactggttggttnccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGt                                                                                                                                                                       >  2:619734‑M2/165‑300 (MQ=255)

                                                                                            ggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCCCTAAATCGt                                                                                                                                                                       >  1:114713‑M2/165‑300 (MQ=255)

                                                                                              taataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGAACGCGCGAATTAACCGTGGTGGGGCGATACCTGAACCAGTAAAACAACTTACTGTAATGGGCGGTAGACCTCTAAATCGt                                                                                                                                                                       >  2:498462‑M2/163‑298 (MQ=255)

                                                                                                   acattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGcaca                                                                                                                                                                  >  2:502828‑M2/158‑298 (MQ=255)

                                                                                                     attacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGct                                                                                                                                                              <  1:503247‑M2/145‑1 (MQ=255)

                                                                                                           catcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGCCCTCTAAATCGTGCACAGGCTCTGGc                                                                                                                                                         >  2:688920‑M2/150‑299 (MQ=255)

                                                                                                           catcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCAGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGCACCAGTAAAACAACTTCCTGTCATGGGCGGTAGCCCTCTAAATCGTACACAGGCTCTGGc                                                                                                                                                         >  2:451194‑M2/150‑299 (MQ=255)

                                                                                                            atcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTAATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGa                                                                                                                                                       >  2:363525‑M2/149‑300 (MQ=255)

                                                                                                            atcccacactgctgaatttgttttcagtggctgcctttttcaaaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCCTGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGa                                                                                                                                                       >  2:362699‑M2/149‑300 (MQ=255)

                                                                                                            actctacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgttgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagagcatgcgtgccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGTCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTTGCGa                                                                                                                                                       <  2:425913‑M2/152‑1 (MQ=255)

                                                                                                                 acactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGAt                                                                                                                                                   <  1:280749‑M2/156‑1 (MQ=255)

                                                                                                                 acactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGAt                                                                                                                                                   <  1:121701‑M2/156‑1 (MQ=255)

                                                                                                                    ctgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCa                                                                                                                                               >  1:512441‑M2/141‑300 (MQ=255)

                                                                                                                            tttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGNACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCaa                                                                                                                                       >  1:182546‑M2/133‑300 (MQ=255)

                                                                                                                            tttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGAGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGNACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCaa                                                                                                                                       >  1:182556‑M2/133‑300 (MQ=255)

                                                                                                                                     agtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGtt                                                                                                                              <  2:657760‑M2/177‑1 (MQ=255)

                                                                                                                                           tgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGNAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACt                                                                                                                        <  2:255498‑M2/183‑1 (MQ=255)

                                                                                                                                             cctttttcacaaagggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAACTAAGTTCGGACTGa                                                                                                                      >  2:147227‑M2/116‑300 (MQ=255)

                                                                                                                                                       aaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAg                                                                                                            <  2:453755‑M2/195‑1 (MQ=255)

                                                                                                                                                          aggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAgtgt                                                                                                         <  1:440772‑M2/198‑1 (MQ=255)

                                                                                                                                                          aggggaaatattgctggatggtggattcaaccagacataaccataggcatatcccattgcttacttgttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATGACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGTTGAGGCGATACCGGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAgtgt                                                                                                         <  1:441103‑M2/198‑1 (MQ=255)

                                                                                                                                                            ggtaaatattactgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTAt                                                                                                       <  2:57076‑M2/200‑1 (MQ=255)

                                                                                                                                                               aaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                    <  1:722919‑M2/203‑1 (MQ=255)

                                                                                                                                                               aaatattgctgtatggtggatgcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                    <  2:755224‑M2/203‑1 (MQ=255)

                                                                                                                                                                aatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactgtttggttaccaacttgtaccagaacatgctggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTTAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                    <  2:225626‑M2/203‑1 (MQ=255)

                                                                                                                                                                 atattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACgg                                                                                                  <  2:746545‑M2/205‑1 (MQ=255)

                                                                                                                                                                        tgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAaga                                                                                           <  1:724414‑M2/212‑1 (MQ=255)

                                                                                                                                                                         gtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCCAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGNCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACt                                                                                                                        >  2:249176‑M2/88‑270 (MQ=255)

                                                                                                                                                                                  attcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTcggactgaaaggatcaagtgtatctgtctcttatacacatctcgc                                                                                 <  1:618788‑M2/222‑23 (MQ=255)

                                                                                                                                                                                      aaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCAtt                                                                             >  1:289748‑M2/75‑300 (MQ=255)

                                                                                                                                                                                      aaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCAtt                                                                             >  1:290232‑M2/75‑300 (MQ=255)

                                                                                                                                                                                          agacataaccatatgcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCACTAcc                                                                          <  1:298075‑M2/229‑1 (MQ=255)

                                                                                                                                                                                          agacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTAcc                                                                          <  1:298177‑M2/229‑1 (MQ=255)

                                                                                                                                                                                          agacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTAcc                                                                          <  1:298553‑M2/229‑1 (MQ=255)

                                                                                                                                                                                           gccataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACAGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTAATGGGCGGTAGAACTCCAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAagtatt                                                                                 >  2:215272‑M2/70‑291 (MQ=255)

                                                                                                                                                                                           gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCattaact                                                                         >  1:543158‑M2/70‑296 (MQ=255)

                                                                                                                                                                                           gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  1:382984‑M2/70‑299 (MQ=255)

                                                                                                                                                                                           gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTAATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTa                                                                            >  2:656750‑M2/70‑296 (MQ=255)

                                                                                                                                                                                           gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAAATTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  1:382985‑M2/70‑299 (MQ=255)

                                                                                                                                                                                           gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGAACGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  1:383224‑M2/70‑299 (MQ=255)

                                                                                                                                                                                           gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCNGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATNAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  2:600727‑M2/70‑299 (MQ=255)

                                                                                                                                                                                             cataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATtacat                                                                         >  1:635234‑M2/68‑295 (MQ=255)

                                                                                                                                                                                             cataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  1:635204‑M2/68‑297 (MQ=255)

                                                                                                                                                                                                aaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGAc                                                                   >  1:800859‑M2/65‑300 (MQ=255)

                                                                                                                                                                                                       ggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCataa                                                             >  2:22004‑M2/58‑299 (MQ=255)

                                                                                                                                                                                                       ggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCata                                                              >  2:22035‑M2/58‑298 (MQ=255)

                                                                                                                                                                                                          atatcctattgcttactgtttggTTACCAACTTGTACCAGAACATGCGTGCCAATGCGCTGACTTACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTTAGGCGATACCTGAACCAGTAAAACAACTGCCGGTCATGGGCGTTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGc                                                         <  1:183571/280‑1 (MQ=255)

                                                                                                                                                                                                          atatcccattgcttactggttggttaccaacttgtaccagaacatgcgtgccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGc                                                         <  1:183141‑M2/246‑1 (MQ=255)

                                                                                                                                                                                                                    gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACTGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTgtgcac                                               <  2:57994‑M2/256‑3 (MQ=255)

                                                                                                                                                                                                                    gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTgtgcac                                               <  1:128207‑M2/256‑3 (MQ=255)

                                                                                                                                                                                                                    gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTgtgcac                                               <  1:25821‑M2/256‑3 (MQ=255)

                                                                                                                                                                                                                          tggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGTCGATACCTGAACCAGTAAAACAATTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGcttctgtgcacaggac                                          <  2:364472‑M2/261‑8 (MQ=255)

                                                                                                                                                                                                                          tggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGcttctgtgcacaggac                                          <  2:364539‑M2/261‑8 (MQ=255)

                                                                                                                                                                                                                            gttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGGAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCATTAACTGGGGACGCATAAGAGCTTCTGTGCACAGGACGtt                                       >  2:110562‑M2/37‑300 (MQ=255)

                                                                                                                                                                                                                              tggttaccaacttgtaccagaacatgctggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTTTCATGGGCGGTAGACCTCTAAATCGTGCACAAGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGc                                     <  1:339197‑M2/266‑1 (MQ=255)

                                                                                                                                                                                                                              tggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGc                                     <  1:339188‑M2/266‑1 (MQ=255)

                                                                                                                                                                                                                                gTTACCAACTTGTACCAGAACATGCGGGCAAATGCGCTGACTGACGAGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAAACGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCCCAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAGGGCTCAAGTGTCTGACGGGCAAAGAGGCAATTATTCATTACTTGGGGACGCATAAGAGCttat                                                     >  2:197291/1‑280 (MQ=255)

                                                                                                                                                                                                                                      aacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTATGCACAGGACGTTGCCGCGGTaa                             >  2:167335‑M2/27‑300 (MQ=255)

                                                                                                                                                                                                                                      aacttataccAGAACATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGTAAGGCTGCTGATGAACT‑GTCC‑TTTATGCATGCGCGGATTACCCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGTGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAATATCGCAGAAATCAAAGTTAAGTTCGGACTGAAAGGATCAAGTGTCTGACGGGCAAAGAGGCAATTATTCAt                                                                              >  2:167054/10‑251 (MQ=255)

                                                                                                                                                                                                                                         ttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTATAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAg                          <  1:607859‑M2/277‑1 (MQ=255)

                                                                                                                                                                                                                                            taccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAAcaggtg                       <  1:772112‑M2/280‑3 (MQ=255)

                                                                                                                                                                                                                                               cAGAACATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCaa                    >  1:196786/1‑300 (MQ=255)

                                                                                                                                                                                                                                                 gAACATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAcc                  <  1:627693/300‑2 (MQ=255)

                                                                                                                                                                                                                                                  aaCATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTTGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAccg                 <  2:432823/300‑3 (MQ=255)

                                                                                                                                                                                                                                                  aaCATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCCAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAccg                 <  2:553804/300‑3 (MQ=255)

                                                                                                                                                                                                                                                   aCATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAccgt                >  1:479506/1‑297 (MQ=255)

                                                                                                                                                                                                                                                       gcgtgCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAAcc            <  2:693221/296‑1 (MQ=255)

                                                                                                                                                                                                                                                              aaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATaa     >  2:151965/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                   gctgaCTGACGCGGAACTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGT‑ATGACCGCGCGAATTAACTTTTGTTAGGCGATACCTTAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATc  <  2:211051/294‑1 (MQ=255)

TGGAACAGGTTAACGCAGGAATGCGCGTAGCCCGTCGGCAGAATCGACCATTTCTGCCATCACCCGGGCAGTTTGTTGCATGGTGCCGGGAAGAAGCATCCGTTACCGCCGGACTGCCAAACGTCAGCGAGCTGGTTGATATGGTTTACGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCC‑AT‑ATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAACCAGCATAAATC  >  NC_012971/762605‑763161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`