BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012971	762,873	T→C	D171D (GAT→GAC)	ECD_RS03820 →	replication protein P

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012971	762,873	0	T	C	98.3%	430.5 / ‑5.8	117	D171D (GAT→GAC)	ECD_RS03820	replication protein P
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (58/57); minor base A (2/0); total (60/57)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

GGAAAACGGGATCACCACGATGGAACAGGTTAACGCAGGAATGCGCGTAGCCCGTCGGCAGAATCGACCATTTCTGCCATCACCCGGGCAGTTTGTTGCATGGTGCCGGGAAGAAGCATCCGTTACCGCCGGACTGCCAAACGTCAGCGAGCTGGTTGATATGGTTTACGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCC‑ATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAACCAGCATAAATC  >  NC_012971/762585‑763161

gacgtgtttcccggtgagtgggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtaagcaggtaataacattacacatccaacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccaganataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTAc                                                                                                                                                                                                                                                                                         >  2:627693‑M2/277‑299 (MQ=255)

 acgtgtttcccggtgagtgggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTAcgg                                                                                                                                                                                                                                                                                       <  1:482619‑M2/25‑2 (MQ=255)

  cgtgtttcccggtgagtgggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacggatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTacggc                                                                                                                                                                                                                                                                                      >  1:544757‑M2/275‑298 (MQ=255)

     gtttcccggtgagtgggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccactgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAattacggcgca                                                                                                                                                                                                                                                                                   >  1:804241‑M2/272‑295 (MQ=255)

            ggtgagtgggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAaggctgc                                                                                                                                                                                                                                                                            >  1:693221‑M2/265‑297 (MQ=255)

            ggtgagtgggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGc                                                                                                                                                                                                                                                                               <  1:215294‑M2/33‑1 (MQ=255)

             gtgagtgggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCaaggctgcc                                                                                                                                                                                                                                                                           >  1:755224‑M2/264‑296 (MQ=255)

               gagtgggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcaaaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccga                                                                                                                                                                                                                                                                         >  1:85781‑M2/262‑294 (MQ=255)

                    ggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccccactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGAtgaac                                                                                                                                                                                                                                                                    >  1:726736‑M2/257‑298 (MQ=255)

                    ggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatacgacaggtgtgtcagtaggtaataacattacacatccaaaacttctgaatttgttttcagtggctgcctttttcacaaaaggggaagtattgctgtctggtggattcaaccggacataaccagaggcatatcaacttgcttactggttggttaccaactcgtaacagaacatgcgggccaaTGCGCTGACTGAAGCGGaattaaggc                                                                                                                                                                                                                                                                                      >  2:183571‑M2/257‑278 (MQ=255)

                    ggttaataaatgccagtcgtcctgccgtgatgaatctgaactcaatggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattaaacatcccacactgctgaatttgttttcagtggctgcctttttaacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGAAGAGGAATTACGGcgcaaggctgccgat                                                                                                                                                                                                                                                                        >  2:183141‑M2/257‑289 (MQ=255)

                     gttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaacagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGatgaact                                                                                                                                                                                                                                                                   >  2:472840‑M2/256‑297 (MQ=255)

                         ataaatgccagtcgtcctgccttgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcatgtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTgccgatgaactgtcc                                                                                                                                                                                                                                                               <  1:445906‑M2/49‑8 (MQ=255)

                         ataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTgccgatgaactgtcc                                                                                                                                                                                                                                                               <  1:445891‑M2/49‑8 (MQ=255)

                              tgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAaggctgccgatgaactgtcctgtat                                                                                                                                                                                                                                                          <  1:662581‑M2/54‑13 (MQ=255)

                               gccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatgntggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAaggctgccgatgaactgtcctgtat                                                                                                                                                                                                                                                          <  2:581004‑M2/54‑13 (MQ=255)

                                  agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtgtattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                       <  2:697769‑M2/57‑16 (MQ=255)

                                  agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                       <  2:414707‑M2/57‑16 (MQ=255)

                                  agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                       <  2:505382‑M2/57‑16 (MQ=255)

                                  agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatagtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                       <  1:770260‑M2/57‑16 (MQ=255)

                                      gtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTacggcgcaaggctgccgatgaactgtcctgtatgaccgcgc                                                                                                                                                                                                                                                  >  2:25821‑M2/239‑280 (MQ=255)

                                      gtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTacggcgcaaggctgccgatgaactgtcctgtatgaccgcgc                                                                                                                                                                                                                                                  >  1:553804‑M2/239‑280 (MQ=255)

                                           gccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGgaattacggcgcaaggctgccgatgaactgtcctgtatgaccgcgcgaatt                                                                                                                                                                                                                                             >  2:106839‑M2/234‑275 (MQ=255)

                                              gtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAAc                                                                                                                                                                                                                                         <  1:116086‑M2/70‑1 (MQ=255)

                                                   gaatctgacctcacttgcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCgtgg                                                                                                                                                                                                                                    <  2:759339‑M2/75‑1 (MQ=255)

                                                    aatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCgtggt                                                                                                                                                                                                                                   >  1:432823‑M2/225‑300 (MQ=255)

                                                    aatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtaagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCgtggt                                                                                                                                                                                                                                   >  2:435108‑M2/225‑300 (MQ=255)

                                                      tctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccanaggcatatcccattgcttactggttggttaccnacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCgtggtg                                                                                                                                                                                                                                  <  2:600089‑M2/77‑1 (MQ=255)

                                                             tcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacaccccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGa                                                                                                                                                                                                                            <  2:775989‑M2/83‑1 (MQ=255)

                                                                        ttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGt                                                                                                                                                                                                               >  1:400451‑M2/205‑300 (MQ=255)

                                                                          gtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTTAACCAGTaa                                                                                                                                                                                                             >  1:211051‑M2/203‑300 (MQ=255)

                                                                          gtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTaa                                                                                                                                                                                                             >  1:210564‑M2/203‑300 (MQ=255)

                                                                          gtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcaaaaaaggggaaatattgctgtatggtggattcaaccagacataaacagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTaa                                                                                                                                                                                                             >  2:482091‑M2/203‑300 (MQ=255)

                                                                                  tcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACtt                                                                                                                                                                                                     >  2:128207‑M2/195‑300 (MQ=255)

                                                                                         accatcctacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagttgctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacntgtaccagaacatgcgggccaaTGCGCTGACTNACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCa                                                                                                                                                                                              <  2:620607‑M2/113‑1 (MQ=255)

                                                                                         accatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCa                                                                                                                                                                                              <  1:425996‑M2/113‑1 (MQ=255)

                                                                                                 acagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGt                                                                                                                                                                                      >  2:556104‑M2/180‑300 (MQ=255)

                                                                                                     atgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggnaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAc                                                                                                                                                                                  <  1:151924‑M2/125‑1 (MQ=255)

                                                                                                             gcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAAt                                                                                                                                                                          <  1:510150‑M2/133‑1 (MQ=255)

                                                                                                             gcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTACTGTCATGGGCGGTAGACCTCTAAAt                                                                                                                                                                          <  1:499127‑M2/133‑1 (MQ=255)

                                                                                                                ggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataancagaggcatatcccattgcttactggttggttnccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGt                                                                                                                                                                       >  2:619734‑M2/165‑300 (MQ=255)

                                                                                                                ggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCCCTAAATCGt                                                                                                                                                                       >  1:114713‑M2/165‑300 (MQ=255)

                                                                                                                  taataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGAACGCGCGAATTAACCGTGGTGGGGCGATACCTGAACCAGTAAAACAACTTACTGTAATGGGCGGTAGACCTCTAAATCGt                                                                                                                                                                       >  2:498462‑M2/163‑298 (MQ=255)

                                                                                                                       acattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGcaca                                                                                                                                                                  >  2:502828‑M2/158‑298 (MQ=255)

                                                                                                                         attacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGct                                                                                                                                                              <  1:503247‑M2/145‑1 (MQ=255)

                                                                                                                               catcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGCCCTCTAAATCGTGCACAGGCTCTGGc                                                                                                                                                         >  2:688920‑M2/150‑299 (MQ=255)

                                                                                                                                atcccacactgctgaatttgttttcagtggctgcctttttcaaaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCCTGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGa                                                                                                                                                       >  2:362699‑M2/149‑300 (MQ=255)

                                                                                                                                actctacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgttgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagagcatgcgtgccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGTCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTTGCGa                                                                                                                                                       <  2:425913‑M2/152‑1 (MQ=255)

                                                                                                                                     acactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGAt                                                                                                                                                   <  1:280749‑M2/156‑1 (MQ=255)

                                                                                                                                     acactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGAt                                                                                                                                                   <  1:121701‑M2/156‑1 (MQ=255)

                                                                                                                                        ctgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCa                                                                                                                                               >  1:512441‑M2/141‑300 (MQ=255)

                                                                                                                                                tttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGNACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCaa                                                                                                                                       >  1:182546‑M2/133‑300 (MQ=255)

                                                                                                                                                         agtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGtt                                                                                                                              <  2:657760‑M2/177‑1 (MQ=255)

                                                                                                                                                               tgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGNAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACt                                                                                                                        <  2:255498‑M2/183‑1 (MQ=255)

                                                                                                                                                                 cctttttcacaaagggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAACTAAGTTCGGACTGa                                                                                                                      >  2:147227‑M2/116‑300 (MQ=255)

                                                                                                                                                                           aaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAg                                                                                                            <  2:453755‑M2/195‑1 (MQ=255)

                                                                                                                                                                              aggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAgtgt                                                                                                         <  1:440772‑M2/198‑1 (MQ=255)

                                                                                                                                                                              aggggaaatattgctggatggtggattcaaccagacataaccataggcatatcccattgcttacttgttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATGACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGTTGAGGCGATACCGGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAgtgt                                                                                                         <  1:441103‑M2/198‑1 (MQ=255)

                                                                                                                                                                                ggtaaatattactgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTAt                                                                                                       <  2:57076‑M2/200‑1 (MQ=255)

                                                                                                                                                                                   aaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                    <  1:722919‑M2/203‑1 (MQ=255)

                                                                                                                                                                                   aaatattgctgtatggtggatgcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                    <  2:755224‑M2/203‑1 (MQ=255)

                                                                                                                                                                                    aatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactgtttggttaccaacttgtaccagaacatgctggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTTAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                    <  2:225626‑M2/203‑1 (MQ=255)

                                                                                                                                                                                     atattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACgg                                                                                                  <  2:746545‑M2/205‑1 (MQ=255)

                                                                                                                                                                                            tgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAaga                                                                                           <  1:724414‑M2/212‑1 (MQ=255)

                                                                                                                                                                                             gtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCCAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGNCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACt                                                                                                                        >  2:249176‑M2/88‑270 (MQ=255)

                                                                                                                                                                                                      attcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTcggactgaaaggatcaagtgtatctgtctcttatacacatctcgc                                                                                 <  1:618788‑M2/222‑23 (MQ=255)

                                                                                                                                                                                                          aaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCAtt                                                                             >  1:289748‑M2/75‑300 (MQ=255)

                                                                                                                                                                                                          aaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCAtt                                                                             >  1:290232‑M2/75‑300 (MQ=255)

                                                                                                                                                                                                              agacataaccatatgcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCACTAcc                                                                          <  1:298075‑M2/229‑1 (MQ=255)

                                                                                                                                                                                                              agacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTAcc                                                                          <  1:298177‑M2/229‑1 (MQ=255)

                                                                                                                                                                                                              agacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTAcc                                                                          <  1:298553‑M2/229‑1 (MQ=255)

                                                                                                                                                                                                               gccataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACAGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTAATGGGCGGTAGAACTCCAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAagtatt                                                                                 >  2:215272‑M2/70‑291 (MQ=255)

                                                                                                                                                                                                               gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCattaact                                                                         >  1:543158‑M2/70‑296 (MQ=255)

                                                                                                                                                                                                               gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  1:382984‑M2/70‑299 (MQ=255)

                                                                                                                                                                                                               gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTAATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTa                                                                            >  2:656750‑M2/70‑296 (MQ=255)

                                                                                                                                                                                                               gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAAATTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  1:382985‑M2/70‑299 (MQ=255)

                                                                                                                                                                                                               gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGAACGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  1:383224‑M2/70‑299 (MQ=255)

                                                                                                                                                                                                               gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCNGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATNAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  2:600727‑M2/70‑299 (MQ=255)

                                                                                                                                                                                                                 cataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATtacat                                                                         >  1:635234‑M2/68‑295 (MQ=255)

                                                                                                                                                                                                                    aaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGAc                                                                   >  1:800859‑M2/65‑300 (MQ=255)

                                                                                                                                                                                                                           ggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCata                                                              >  2:22035‑M2/58‑298 (MQ=255)

                                                                                                                                                                                                                              atatcctattgcttactgtttggTTACCAACTTGTACCAGAACATGCGTGCCAATGCGCTGACTTACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTTAGGCGATACCTGAACCAGTAAAACAACTGCCGGTCATGGGCGTTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGc                                                         <  1:183571/280‑1 (MQ=255)

                                                                                                                                                                                                                              atatcccattgcttactggttggttaccaacttgtaccagaacatgcgtgccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGc                                                         <  1:183141‑M2/246‑1 (MQ=255)

                                                                                                                                                                                                                                        gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACTGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTgtgcac                                               <  2:57994‑M2/256‑3 (MQ=255)

                                                                                                                                                                                                                                        gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTgtgcac                                               <  1:128207‑M2/256‑3 (MQ=255)

                                                                                                                                                                                                                                              tggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGTCGATACCTGAACCAGTAAAACAATTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGcttctgtgcacaggac                                          <  2:364472‑M2/261‑8 (MQ=255)

                                                                                                                                                                                                                                              tggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGcttctgtgcacaggac                                          <  2:364539‑M2/261‑8 (MQ=255)

                                                                                                                                                                                                                                                gttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGGAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCATTAACTGGGGACGCATAAGAGCTTCTGTGCACAGGACGtt                                       >  2:110562‑M2/37‑300 (MQ=255)

                                                                                                                                                                                                                                                  tggttaccaacttgtaccagaacatgctggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTTTCATGGGCGGTAGACCTCTAAATCGTGCACAAGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGc                                     <  1:339197‑M2/266‑1 (MQ=255)

                                                                                                                                                                                                                                                  tggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGc                                     <  1:339188‑M2/266‑1 (MQ=255)

                                                                                                                                                                                                                                                    gTTACCAACTTGTACCAGAACATGCGGGCAAATGCGCTGACTGACGAGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAAACGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCCCAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAGGGCTCAAGTGTCTGACGGGCAAAGAGGCAATTATTCATTACTTGGGGACGCATAAGAGCttat                                                     >  2:197291/1‑280 (MQ=255)

                                                                                                                                                                                                                                                          aacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTATGCACAGGACGTTGCCGCGGTaa                             >  2:167335‑M2/27‑300 (MQ=255)

                                                                                                                                                                                                                                                             ttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTATAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAg                          <  1:607859‑M2/277‑1 (MQ=255)

                                                                                                                                                                                                                                                                taccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAAcaggtg                       <  1:772112‑M2/280‑3 (MQ=255)

                                                                                                                                                                                                                                                                   cAGAACATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCaa                    >  1:196786/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                     gAACATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAcc                  <  1:627693/300‑2 (MQ=255)

                                                                                                                                                                                                                                                                      aaCATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTTGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAccg                 <  2:432823/300‑3 (MQ=255)

                                                                                                                                                                                                                                                                       aCATGCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAAccgt                >  1:479506/1‑297 (MQ=255)

                                                                                                                                                                                                                                                                                  aaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATaa     >  2:151965/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                       gctgaCTGACGCGGAACTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACTTTTGTTAGGCGATACCTTAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATc  <  2:211051/294‑1 (MQ=255)

GGAAAACGGGATCACCACGATGGAACAGGTTAACGCAGGAATGCGCGTAGCCCGTCGGCAGAATCGACCATTTCTGCCATCACCCGGGCAGTTTGTTGCATGGTGCCGGGAAGAAGCATCCGTTACCGCCGGACTGCCAAACGTCAGCGAGCTGGTTGATATGGTTTACGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCC‑ATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAACCAGCATAAATC  >  NC_012971/762585‑763161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`