Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,174,041	A→T	E320D (GAA→GAT)	birA →	DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,174,041	0	A	T	100.0%	19.6 / NA	9	E320D (GAA→GAT)	birA	DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/3);  total (6/3)

GGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGT  >  NC_000913/4173885‑4174245                                                                                                                                                             |                                                                                                                                                                                                             ggATAATTTTATTAATCGCCCAGTGAAACTTATCATTCGTGATAAAGAAAGATATGGCCTTTAACGCGGAATAGACACACAGGGGGCTTTATTACTTGAGCACGATGGAATAATAAAACCCTGGCTGGGCGGTGCAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTTTTCGCTCCCATGCAAATTATTTg                                                                                                                                                                     >  1:136806/1‑198 (MQ=255)              aaTCGCCCAGTGAAACTTATCATTGGCGATAAAGAAATATTTGGCATTTAACGCGGCATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACACTGGATGGGCGGTGAAATATCCCTGCGTCGTGCAGATAAATAAGAAAGGGGAGTATTAGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACg                                                                                                    >  1:119562/1‑250 (MQ=255)                      aGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCGCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACg                                                                                                    <  1:123841/242‑1 (MQ=255)                               tATCATTGNTNNNANNGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTNTTACTTGAGCAGGATNGCNTAATNAAANCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGt                                                                                                                                                                  >  2:181189/1‑171 (MQ=255)                               tATCATTGGTGATAAAGAAATATTTGGCATGTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGGGAAATATCCCTGCGTAGTGCAGATAAAGAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGt                                                                                                                                                                  <  1:181189/171‑1 (MQ=255)                                             aaGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTATTCTTCATGATGAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGcc                                                                    <  1:253851/251‑1 (MQ=255)                                                                                cNGGGGGCTTTATTACTTGAGCAGGATGGTATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCATAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAg                                                                                                                         >  1:206997/1‑163 (MQ=255)                                                                                                     cAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTAc                                                                                                                                                 >  1:205599/1‑118 (MQ=255)                                                                                                                                    gTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGt  >  1:15410/1‑230 (MQ=255)                                                                                                                                                             |                                                                                                                                                                                                             GGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGT  >  NC_000913/4173885‑4174245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑