Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,123,930	A→T	V176E (GTA→GAA)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,930	0	A	T	100.0%	16.7 / NA	7	V176E (GTA→GAA)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (3/4);  total (3/4)

CCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTG  >  NC_000913/4123688‑4124135                                                                                                                                                                                                                                                   |                                                                                                                                                                                                              ccATCACATCGCTATGGCAGAAGCCAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGCCACAGCGGCATCTCTTCGGGTCCCGCTATACAACCCATCCGCTTATTCCCTTGCTCATATACATAATTTTCTGCATc                                                                                                                                                                                                        >  1:209308/1‑250 (MQ=255)                                              gATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGTGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAAc                                                                                                                                                                          <  1:98743/235‑1 (MQ=255)                                              gATCAAGCAGCGGCTGCATCGCTTGGCTCCCGGCTGCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGTCGGTAGTGACAGCGCGACATCTCTGCGGAACCGGCTATACAGCCAATCCGTGTATGCCCTTGCTCATATAAATAATTCTCGGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGcagctccagc                                                                                                                                                          <  1:46644/251‑1 (MQ=255)                                                                                                                                 ccGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGAAGTGACACAGCGGCATCTCTTCGGGACCGGCTATAAAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCCGATTGTCGCTATGAACTGTAGGCAGCTCCCGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCCATGCTTGCATCAAACGGCAGCCTTGAAcc                                                                       >  1:96568/1‑251 (MQ=255)                                                                                                                                                                                   cGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCGTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATaaacg                     <  1:246942/251‑3 (MQ=255)                                                                                                                                                                                                 gACCGGCTATACAGCCAAACCGTTTATGCCCTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGCTCGCCATCACCATCGGCGGCAGATTACGCTGGTCCTCAATGCTGGCATCAATCGGTAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATc                                  <  1:119720/224‑1 (MQ=255)                                                                                                                                                                                                      gCTATACAGCCAATCCGTTTATGCCTTTGCTCATATAAATAATTTTCTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTg  >  1:83195/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                              CCATCACATCGCTATGGCAGAAGACAGCAGTAGGCGGTTGTGGAAGATCAAGCAGCTGCTGCATCGCTTTGCTTCCGGCTTCGAAGGTGAAGTCGCCACGGGCGATGTATTGCGGATCAACCATAATGCCGCAGCGACGCAGCGCCTGAACATAGCCTTGCAGGCGGTAGTGACACAGCGGCATCTCTTCGGGACCGGCTATACAGCCAATCCGTTTATGCCCTTGCTCATATAAATAATTTACTGCATCAAATGCGGCGGTCAGATTGTCGATATGAACTGTAGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTG  >  NC_000913/4123688‑4124135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑