Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	34.7 / NA	13	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/7);  total (6/7)

CACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGA  >  NC_000913/1905573‑1905977                                                                                                                                                                                             |                                                                                                                                                                                                                         cacaTCAATCGCTATGCCTGCATGAACGCGAAAACCAGCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCTCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAc                                                                                                                                                                                <  1:228526/231‑1 (MQ=255)                nccTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAt                                                                                                                                                                                       >  1:164307/2‑209 (MQ=255)                 ccTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgcn                                                                                                                                            <  1:166337/251‑2 (MQ=255)                                                       ngTATGCATGTTTGCTGGGGGCGATGATGTGGTTTATGGAAACCCCGGTCCGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGAATGTCGATGGATGCATTTTCTGCATCCATTGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGTAGAATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAAACCTGACGCCGCTGATCGGCTGGGGTATGGGCATGTTAGCCAGCCGGt                                                                                                      >  1:25661/2‑251 (MQ=255)                                                                           nnnnnnatnNNNNTTANGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAt                                                                                                                                                                                       <  2:164307/144‑1 (MQ=255)                                                                              atgatgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCAATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTgg                                                                               <  1:36968/251‑1 (MQ=255)                                                                                 atgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGATAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTGTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCGTGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGAtt                                                                            <  1:266714/251‑1 (MQ=255)                                                                                            ggATACCCCGGTCAGGACATTGTCATGAATATCACTGATACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAACTTTTCTGACGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgct                                                                 >  1:85850/1‑251 (MQ=255)                                                                                                 ccccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGGTTGGTATGTCGATGGATGCATTTTCTTCATCAATCGGTAAAGATGCCGCCCTCCATAAACCGAAATTATCTGAAGCATTGCGAAc                                                                                                                                                                                <  1:263601/135‑1 (MQ=255)                                                                                                          ggACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGCTGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTc                                                    >  1:38345/1‑250 (MQ=255)                                                                                                          ggACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagccag                                                                                                           >  1:103133/1‑195 (MQ=255)                                                                                                                                    cTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGACACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTTTTGGGGGtttt                         >  1:225974/1‑251 (MQ=255)                                                                                                                                                            cGATGGATGCATTTGCTGCATCAATCGGTAATGATGCCCCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGTCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCGGCCGGTGTGTCGTTGAATGGAACCACTGGATTGCGTTTGTGCTTCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATgatgaagatga  <  1:231542/250‑1 (MQ=255)                                                                                                                                                                                             |                                                                                                                                                                                                                         CACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGA  >  NC_000913/1905573‑1905977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑