Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	803,662	C→A	L54I (CTT→ATT)	ybhJ →	putative hydratase YbhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	803,662	0	C	A	100.0%	39.6 / NA	14	L54I (CTT→ATT)	ybhJ	putative hydratase YbhJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (3/11);  total (3/11)

CACGGTGCGCAGATATCTGGAGCATTTGATGATCAAGTTATCTGAAAAAGGCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATC  >  NC_000913/803475‑803892                                                                                                                                                                                            |                                                                                                                                                                                                                                       cACGGTGCGCAGATATCTGGAGCATTTGATGATCAAGTTATCTGAAAAAGGCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTGCAGACCg                                                                                                                                                                         <  1:202269/251‑1 (MQ=255)                                        aTCTGAAAAAGGCGTGTTTATCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCGCTCTTCTCATAATACTTCCTGAAATATGGATAAAAGTAAAATTAAGTTTGATTCATTAGCCTCTCAAGATATTACCTTTGTTGGTATTGTACAGAc                                                                                                                                                                           <  1:253307/210‑1 (MQ=255)                                               aaaGGCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACTATATTACCTTGGTGGGTATTGGACAGTCCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGAc                                                                                                                           <  1:121000/251‑1 (MQ=255)                                                            tcGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATGATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTACGTGCTGACCAACTGCCATAac                                                                                                              >  1:135079/1‑251 (MQ=255)                                                                                       aagaaCATGTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGAACGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTAtt                                                                                   <  1:129645/251‑1 (MQ=255)                                                                                          aaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAAc                                                                                <  1:99778/251‑1 (MQ=255)                                                                                                           aTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATGCTCTCTTCGCATAATTCTTCCGGAAATAGGGATAAAATTAAAATTAATGTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGTTGTTGTACAGATCGCTAAAGCGTCCGGTAGGGAAGGTTTCCCTCTGCCGTATGTGCTGACCAACTGCCATAGCTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGtttt                                                               <  1:243551/251‑1 (MQ=255)                                                                                                                    gaagaagCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATc                                                      <  1:152670/251‑1 (MQ=255)                                                                                                                         agCCAAAAAAGGCACTATTGCCTGGGCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACCATATTCCCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTCTGGAACGTTTCCCGCTGCCGGATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATAACCATGTTTTTGGTTTATcggcgg                                                 >  1:135628/1‑251 (MQ=255)                                                                                                                              aaaaaGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAg                                            <  1:137061/251‑1 (MQ=255)                                                                                                                                                  tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCNGCTNNNNNAtnnnnnnnnnn                                                                                                                         >  2:166020/1‑144 (MQ=255)                                                                                                                                                  tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAgn                                           <  1:166020/232‑2 (MQ=255)                                                                                                                                                                        cGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCATCTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatc  <  1:245466/251‑1 (MQ=255)                                                                                                                                                                                  tatGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTGGGTATTGTACGGACCGCGAAAGCGTCCGGGAGGGATCGTGTCCCGCTGCCGGATGTGTTTACCAACTGCGAGATTGCACTCTTCGCCCTCGGCGGCACTATGAGCGGTGATTATTGTGTGTTTGGTTTATCGGCGGCCCGGCGTTATGGCGGTAGTTTTGTGCCTCCGCATATTGCggn      <  1:29657/237‑2 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                                                                       CACGGTGCGCAGATATCTGGAGCATTTGATGATCAAGTTATCTGAAAAAGGCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATC  >  NC_000913/803475‑803892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑