breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,645 G→A W282* (TGG→TAG)  thrB → homoserine kinase
RA 71,170 T→C S262P (TCG→CCG)  araC → DNA‑binding transcriptional dual regulator AraC
RA 81,044 C→T L305L (CTG→CTA leuB ← 3‑isopropylmalate dehydrogenase
RA 81,102 G→A S286L (TCG→TTG)  leuB ← 3‑isopropylmalate dehydrogenase
RA 85,203 C→T P279L (CCG→CTG)  leuO → DNA‑binding transcriptional dual regulator LeuO
RA 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
JC JC 168,803 IS1 (+) +9 bp coding (1320‑1328/2244 nt) fhuA → ferrichrome outer membrane transporter/phage receptor
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
MC JC 366,181 Δ93 bp coding (33‑125/3075 nt) lacZ ← beta‑galactosidase
RA 367,573 G→A intergenic (‑63/+14) lacI ← / ← mhpR DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional activator MhpR
RA 456,711 C→G P12R (CCC→CGC)  clpP → ATP‑dependent Clp protease proteolytic subunit
RA 463,184 C→T L424L (CTG→TTG)  ppiD → periplasmic folding chaperone
RA 472,849 A→G G84G (GGA→GGG glnK → nitrogen regulatory protein PII‑2
RA 495,622 C→T T168I (ACC→ATC)  htpG → chaperone protein HtpG
RA 525,532 T→C C758R (TGC→CGC)  rhsD → protein RhsD
RA 525,576 G→C Q772H (CAG→CAC rhsD → protein RhsD
RA 525,585 3 bp→CGG coding (2325‑2327/4281 nt) rhsD → protein RhsD
RA 525,591 C→T H777H (CAC→CAT rhsD → protein RhsD
RA 525,594 A→G A778A (GCA→GCG rhsD → protein RhsD
RA 525,603 G→A E781E (GAG→GAA rhsD → protein RhsD
RA 525,636 C→T S792S (AGC→AGT rhsD → protein RhsD
RA 534,742 G→A G276D (GGT→GAT)  gcl → glyoxylate carboligase
RA 547,891 C→T L404L (CTC→CTT fdrA → putative oxamate carbamoyltransferase FdrA
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 582,597 C→T intergenic (+500/‑278) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 694,941 G→A L5L (CTC→CTT miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 780,624 C→T noncoding (71/76 nt) lysT → tRNA‑Lys
RA 789,580 C→A E134* (GAA→TAA)  galK ← galactokinase
RA 790,832 Δ1 bp coding (198/1047 nt) galT ← galactose‑1‑phosphate uridylyltransferase
RA 822,527 C→T E405K (GAA→AAA)  clsB ← cardiolipin synthase B
RA 854,560 A→G N305S (AAC→AGC)  ybiR → putative transporter YbiR
RA 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 884,892 C→T S407F (TCT→TTT)  mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 905,283 G→T G124V (GGC→GTC)  amiD → N‑acetylmuramoyl‑L‑alanine amidase D
RA 908,169 C→T R38R (AGG→AGA ybjT ← putative NAD(P)‑binding protein YbjT
RA 909,990 C→T E354K (GAG→AAG)  poxB ← pyruvate oxidase
RA 922,954 G→T A14A (GCG→GCT clpS → specificity factor for ClpA‑ClpP chaperone‑protease complex
RA 930,086 C→T A293T (GCC→ACC)  cydD ← glutathione/L‑cysteine ABC exporter subunit CydD
JC JC 1,085,983 IS1 (+) +8 bp coding (992‑999/1065 nt) phoH → ATP‑binding protein PhoH
RA 1,114,320 C→T A391T (GCA→ACA)  mdtG ← efflux pump MdtG
RA 1,123,823 2 bp→AG coding (231‑232/648 nt) grxB ← reduced glutaredoxin 2
RA 1,147,792 T→A S58T (TCG→ACG)  plsX → putative phosphate acyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,194,922 G→A T10T (ACC→ACT rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
RA 1,253,551 G→A A801V (GCC→GTC)  ycgV ← putative autotransporter adhesin YcgV
RA 1,290,118 G→A E292K (GAA→AAA)  rssA → putative patatin‑like phospholipase RssA
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,308,684 T→C intergenic (‑39/‑104) clsA ← / → yciY cardiolipin synthase A/uncharacterized protein YciY
RA 1,331,059 2 bp→AT coding (12‑13/2598 nt) topA → DNA topoisomerase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,386,348 A→G M280V (ATG→GTG)  ycjF → DUF697 domain‑containing inner membrane protein YcjF
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
RA 1,426,980 A→G N176S (AAT→AGT)  ynaA → putative prophage tail length tape measure domain‑containing protein YnaA
RA 1,428,150 G→A L193L (CTG→TTG)  insH5 ← IS5 family transposase and trans‑activator
RA 1,489,491 G→A L420L (CTG→CTA aldA → aldehyde dehydrogenase A
RA 1,494,958 A→T S38R (AGT→AGA ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D
RA 1,629,247 C→T I11I (ATC→ATT ydfZ → putative selenoprotein YdfZ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,726,446 G→A E142K (GAA→AAA)  nemR → DNA‑binding transcriptional repressor NemR
RA 1,748,841 C→T R240K (AGA→AAA)  ydhT ← uncharacterized protein YdhT
RA 1,751,024 C→A intergenic (‑11/+53) ydhX ← / ← ydhW putative 4Fe‑4S ferredoxin‑like protein YdhX/uncharacterized protein YdhW
RA 1,848,300 2 bp→TT coding (376‑377/552 nt) ydjA ← putative oxidoreductase YdjA
RA 1,866,507 G→A intergenic (‑35/‑401) mipA ← / → yeaG MltA‑interacting protein/protein kinase YeaG
JC JC 1,879,829 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,962,172 C→T A67A (GCC→GCT yecT → protein YecT
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,002,087 C→T noncoding (20/195 nt) fliX ← small RNA FliX
RA 2,008,395 C→T A40V (GCT→GTT)  yedE → putative selenium transporter YedE
RA 2,010,068 C→T T83I (ACC→ATC)  yedK → genome maintenance protein
RA 2,023,559 A→G T223A (ACT→GCT)  fliR → flagellar biosynthesis protein FliR
RA 2,027,332 T→A E229V (GAA→GTA)  dgcQ ← diguanylate cyclase DgcQ
RA 2,029,550 C→T R4R (CGC→CGT yedA → putative transporter YedA
RA 2,031,160 C→T E386E (GAG→GAA dcm ← DNA‑cytosine methyltransferase
RA 2,032,183 C→T W45* (TGG→TGA dcm ← DNA‑cytosine methyltransferase
RA 2,033,648 C→T intergenic (‑227/‑1) drpB ← / → rseX cell division protein DrpB/small regulatory RNA RseX
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,278 G→A intergenic (+160/‑97) msrQ → / → zinT membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
RA 2,046,403 C→T A489V (GCT→GTT)  yeeJ → inverse autotransporter adhesin
RA 2,052,885 G→A intergenic (+871/‑758) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
RA 2,072,022 (G)5→4 coding (484/3120 nt) flu → self recognizing antigen 43 (Ag43) autotransporter
RA 2,142,598 G→A L117L (CTC→CTT udk ← uridine/cytidine kinase
JC JC 2,168,341 IS5 (–) +4 bp intergenic (‑35/‑368) yegR ← / → yegS uncharacterized protein YegR/lipid kinase YegS
RA 2,171,384 A→C K285Q (AAG→CAG)  insF5 → IS3 element protein InsF
MC JC 2,171,429 Δ5,958 bp IS3‑mediated [gatR]gatY [gatR], gatD, gatB, gatA, gatZ, gatY
RA 2,233,609 C→T Q4* (CAG→TAG)  yeiS → DUF2542 domain‑containing protein YeiS
RA 2,242,247 C→T G241R (GGG→AGG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,247,134 A→C Y467D (TAC→GAC)  lysP ← lysine:H(+) symporter
RA 2,264,241 T→C F127L (TTT→CTT)  setB → sugar exporter SetB
RA 2,281,855 T→C G408G (GGT→GGC radD → putative DNA repair helicase RadD
RA 2,281,954 C→A N441K (AAC→AAA radD → putative DNA repair helicase RadD
RA 2,350,932 T→C D27G (GAC→GGC)  glpQ ← glycerophosphoryl diester phosphodiesterase GlpQ
RA 2,402,846 A→G F334L (TTC→CTC)  nuoC ← NADH:quinone oxidoreductase subunit CD
RA 2,404,402 G→A S71L (TCG→TTG)  nuoB ← NADH:quinone oxidoreductase subunit B
RA 2,556,984 G→A T105I (ACC→ATC)  eutC ← ethanolamine ammonia‑lyase subunit beta
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,609,473 G→A L329L (CTG→CTA hyfG → hydrogenase 4 catalytic subunit HyfG
RA 2,661,790 G→A S114S (AGC→AGT iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,681,850 G→A A299V (GCG→GTG)  yphG ← DUF5107 domain‑containing protein YphG
RA 2,774,947 G→A G544S (GGT→AGT)  yfjW → uncharacterized protein YfjW
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,880,635 T→C L138L (TTA→TTG casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,949,982 G→A T247T (ACG→ACA argA → N‑acetylglutamate synthase
RA 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ
RA 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II
RA 3,087,508 C→T P268L (CCA→CTA)  metK → methionine adenosyltransferase
MC JC 3,131,341 Δ16,739 bp IS5‑mediated [yghQ][gpr] 18 genes
RA 3,167,148 G→A H191Y (CAT→TAT)  ygiS ← putative deoxycholate binding periplasmic protein
RA 3,187,471 C→A pseudogene (40/2434 nt) yqiG → putative outer membrane usher protein YqiG
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,253,635 G→A R194C (CGT→TGT)  yhaJ ← DNA‑binding transcriptional activator YhaJ
RA 3,279,276 C→G F121L (TTC→TTG kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,486,178 A→G H20R (CAC→CGC)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,511,420 C→T M1M (ATG→ATA) † yhfY ← PRD domain‑containing protein YhfY
RA 3,511,466 C→T V293V (GTG→GTA yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,515,876 C→T G13D (GGC→GAC)  dam ← DNA adenine methyltransferase
RA 3,535,436 C→T V145I (GTA→ATA)  envZ ← sensor histidine kinase EnvZ
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,705,350 C→T W147* (TGG→TGA dppB ← dipeptide ABC transporter membrane subunit DppB
RA 3,748,508 (A)8→7 coding (1425/1497 nt) lyxK → L‑xylulose kinase
RA 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH
RA 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,841,587 G→A G51G (GGC→GGT nepI ← purine ribonucleoside exporter
RA 3,926,991 G→A L5L (CTG→TTG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,934,947 A→G E390E (GAA→GAG rbsA → ribose ABC transporter ATP binding subunit
RA 3,935,143 C→T P456S (CCA→TCA)  rbsA → ribose ABC transporter ATP binding subunit
RA 3,966,530 2 bp→TT coding (114‑115/1260 nt) rho → transcription termination factor Rho
JC JC 4,044,847 IS5 (+) +4 bp coding (649‑652/1431 nt) yihF → DUF945 domain‑containing protein YihF
RA 4,083,569 C→T A752T (GCA→ACA)  fdoG ← formate dehydrogenase O subunit alpha
JC JC 4,124,494 IS2 (+) +5 bp intergenic (‑38/+114) cytR ← / ← priA DNA‑binding transcriptional repressor CytR/primosomal protein N'
RA 4,164,738 A→G Q367R (CAA→CGA)  btuB → cobalamin outer membrane transporter
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,322,288 A→G F45F (TTT→TTC phnF ← putative transcriptional regulator PhnF
RA 4,325,000 C→T G56S (GGC→AGC)  phnC ← phosphonate/phosphate ABC transporter ATP binding subunit
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP
RA 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,476,605 G→A S390N (AGC→AAC)  yjgL → protein YjgL
RA 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,620,453 C→T A284V (GCC→GTC)  deoB → phosphopentomutase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 525643 526517–525873 231–875 56 [54] [54] 57 rhsD protein RhsD
* * ÷ NC_000913 1196302 1211473 15172 57 [53] [55] 57 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 3423817–3424234 3424462–3424238 5–646 56 [55] [52] 57 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 244134 =222 (1.050)288 (1.420) 210/430 0.0 56.9% intergenic (+13/‑193) gmhA/yafJ D‑sedoheptulose 7‑phosphate isomerase/putative glutamine amidotransferase YafJ
?NC_000913 = 377392 224 (1.110)intergenic (+81/+143) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 249545291 (1.380)275 (1.350) 195/434 0.0 48.6% intergenic (+1411/‑1353) rayT/dinB REP‑associated tyrosine transposase/DNA polymerase IV
?NC_000913 373870 = 299 (1.470)coding (950/951 nt)
coding (3/1014 nt)
mhpF
mhpE
acetaldehyde dehydrogenase (acetylating) MhpF
4‑hydroxy‑2‑oxovalerate aldolase
* ? NC_000913 = 2579076 (0.030)190 (0.940) 154/432 0.2 98.5% intergenic (+8/+16) crl/insB9 RNA polymerase holoenzyme assembly factor Crl/IS1 family transposase B
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl RNA polymerase holoenzyme assembly factor Crl
* ? NC_000913 = 12994980 (0.000)89 (0.430) 86/440 1.7 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein