breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 1,903 G→A E523K (GAA→AAA)  thrA → fused aspartate kinase/homoserine dehydrogenase 1
RA 10,696 C→T A221T (GCC→ACC)  yaaW ← putative enzyme‑specific chaperone YaaW
RA 23,501 C→T L371L (CTG→TTG)  ileS → isoleucine‑‑tRNA ligase
RA 31,683 C→T N289N (AAC→AAT carB → carbamoyl‑phosphate synthetase large subunit
RA 42,108 G→A intergenic (‑177/‑295) caiT ← / → fixA L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA
RA 59,968 C→T V127I (GTC→ATC)  rluA ← 23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
RA 76,249 G→A Q351* (CAA→TAA)  sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 88,571 G→A V182M (GTG→ATG)  cra → DNA‑binding transcriptional dual regulator Cra
RA 113,695 G→A W84* (TGG→TGA guaC → GMP reductase
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
RA 157,585 C→T D50N (GAT→AAT)  folK ← 2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase
RA 224,625 T→A noncoding (855/1542 nt) rrsH → 16S ribosomal RNA
MC JC 366,181 Δ93 bp coding (33‑125/3075 nt) lacZ ← beta‑galactosidase
MC JC 402,391 Δ723 bp coding (645‑1367/1416 nt) phoA → alkaline phosphatase
RA 466,148 G→A P389S (CCA→TCA)  ybaE ← uncharacterized protein YbaE
RA 469,271 G→A G134E (GGG→GAG)  mdlA → ABC transporter family protein MdlA
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 502,232 G→A T336I (ACC→ATC)  ybaL ← putative transporter YbaL
RA 662,269 C→T D4N (GAT→AAT)  lipB ← lipoyl(octanoyl) transferase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 784,677 G→A A49A (GCC→GCT zitB ← Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
RA 881,531 A→T I269F (ATC→TTC)  dacC → D‑alanyl‑D‑alanine carboxypeptidase DacC
RA 1,015,891 C→T intergenic (+9/+61) rmf → / ← fabA ribosome modulation factor/beta‑hydroxyacyl‑acyl carrier protein dehydratase/isomerase
JC JC 1,091,173 IS5 (–) +4 bp coding (1114‑1117/2424 nt) pgaA ← partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin
RA 1,137,212 C→T R313R (CGC→CGT flgI → flagellar P‑ring protein
RA 1,138,130 C→T P254S (CCG→TCG)  flgJ → putative peptidoglycan hydrolase FlgJ
RA 1,147,196 C→T P135S (CCG→TCG)  yceD → DUF177 domain‑containing protein YceD
RA 1,156,913 G→A G46D (GGT→GAT)  ycfH → putative metal‑dependent hydrolase YcfH
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,174,883 C→T A95T (GCG→ACG)  ycfT ← inner membrane protein YcfT
RA 1,175,517 A→G T31A (ACC→GCC)  lolC → lipoprotein release complex ‑ inner membrane subunit
RA 1,188,861 C→T V306I (GTA→ATA)  phoQ ← sensor histidine kinase PhoQ
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,191,644 C→T E115K (GAA→AAA)  purB ← adenylosuccinate lyase
RA 1,197,637 C→T G201S (GGT→AGT)  ymfE ← uncharacterized protein YmfE
JC JC 1,199,869 IS3 (+) +3 bp coding (936‑938/1128 nt) intE ← putative integrase
RA 1,235,755 C→T S496N (AGC→AAC)  ycgB ← PF04293 family protein YcgB
RA 1,239,343 C→T T155T (ACC→ACT dadX → alanine racemase 2
RA 1,250,264 C→T G192D (GGT→GAT)  dhaK ← dihydroxyacetone kinase subunit K
RA 1,257,135 C→T A226A (GCG→GCA ychF ← redox‑responsive ATPase YchF
RA 1,268,367 C→T S68F (TCT→TTT)  kdsA → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase
RA 1,269,702 C→T intergenic (+313/+1) rdlA → / ← ldrB putative antisense regulatory RNA RdlA/small toxic polypeptide LdrB
RA 1,282,627 C→T Q922* (CAG→TAG)  narG → nitrate reductase A subunit alpha
RA 1,290,669 G→A W143* (TGG→TAG)  rssB → regulator of RpoS
JC JC 1,301,050 IS1 (+) +9 bp intergenic (+503/‑124) insH21 → / → oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,321,672 C→T T425T (ACG→ACA trpE ← anthranilate synthase subunit TrpE
RA 1,322,759 G→A A63V (GCT→GTT)  trpE ← anthranilate synthase subunit TrpE
RA 1,335,123 C→T intergenic (+294/‑25) cysB → / → ymiA DNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA
RA 1,336,353 C→T P175S (CCA→TCA)  acnA → aconitate hydratase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,384,652 A→G D179G (GAC→GGC)  ycjX → DUF463 domain‑containing protein YcjX
RA 1,387,908 C→T P397S (CCG→TCG)  tyrR → DNA‑binding transcriptional dual regulator TyrR
RA 1,395,566 C→T S119S (TCG→TCA ynaI ← small conductance mechanosensitive channel YnaI
RA 1,399,069 C→T G153D (GGC→GAC)  fnr ← DNA‑binding transcriptional dual regulator FNR
JC JC 1,399,590 IS5 (+) +4 bp intergenic (‑64/+128) fnr ← / ← ogt DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,401,893 G→A Q455* (CAA→TAA)  abgB ← p‑aminobenzoyl‑glutamate hydrolase subunit B
RA 1,405,569 C→T R277W (CGG→TGG)  abgR → putative LysR‑type DNA‑binding transcriptional regulator AbgR
RA 1,418,807 C→T T46I (ACT→ATT)  sieB → phage superinfection exclusion protein
RA 1,419,414 C→T G15S (GGT→AGT)  ydaG ← uncharacterized protein YdaG
RA 1,428,107 C→T G207D (GGT→GAT)  insH5 ← IS5 family transposase and trans‑activator
RA 1,430,310 C→T A421V (GCT→GTT)  stfR → putative prophage side tail fiber protein StfR
RA 1,441,765 C→T intergenic (‑22/+89) hslJ ← / ← ldhA lipoprotein implicated in Novobiocin resistance/D‑lactate dehydrogenase
RA 1,453,992 G→A W22* (TGG→TGA paaA → phenylacetyl‑CoA 1,2‑epoxidase, monooxygenase subunit
RA 1,460,345 C→T A431V (GCC→GTC)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase
RA 1,491,105 C→T T68T (ACC→ACT cybB → superoxide oxidase
RA 1,501,974 C→T T138I (ACC→ATC)  tehB → tellurite methyltransferase
RA 1,514,985 C→T A75V (GCG→GTG)  ydcV → putative ABC transporter membrane subunit YdcV
RA 1,520,526 C→T R89C (CGC→TGC)  mcbR → DNA‑binding transcriptional dual regulator McbR
RA 1,531,318 C→T intergenic (‑814/‑498) yncO ← / → ydcC protein YncO/H repeat‑associated putative transposase YdcC
RA 1,534,321 C→T P100S (CCA→TCA)  nhoA → arylamine N‑acetyltransferase
RA 1,536,304 C→T G105D (GGC→GAC)  narV ← nitrate reductase Z subunit gamma
RA 1,587,323 C→T A138T (GCC→ACC)  ydeQ ← putative fimbrial adhesin protein YdeQ
RA 1,597,364 T→C intergenic (‑4922/+1253) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,626,475 C→T R302R (CGG→CGA dcp ← peptidyl‑dipeptidase Dcp
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,715,661 C→T D77N (GAT→AAT)  pdxY ← pyridoxal kinase 2
RA 1,738,631 T→C L263P (CTG→CCG)  purR → DNA‑binding transcriptional repressor PurR
RA 1,743,164 C→T E27K (GAG→AAG)  ribC ← riboflavin synthase
RA 1,776,100 G→A E172K (GAA→AAA)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,786,965 C→T A50T (GCC→ACC)  ppsA ← phosphoenolpyruvate synthetase
RA 1,790,949 C→T G99D (GGC→GAC)  selO ← protein adenylyltransferase SelO
RA 1,791,057 C→T G63D (GGT→GAT)  selO ← protein adenylyltransferase SelO
RA 1,795,562 C→T D795N (GAT→AAT)  pheT ← phenylalanine‑‑tRNA ligase subunit beta
RA 1,798,171 C→T D258N (GAC→AAC)  pheS ← phenylalanine‑‑tRNA ligase subunit alpha
RA 1,812,346 C→T G263R (GGA→AGA)  ydjO ← protein YdjO
RA 1,828,654 C→T G361R (GGG→AGG)  astD ← aldehyde dehydrogenase
RA 1,844,146 C→T A242A (GCG→GCA ynjI ← DUF1266 domain‑containing protein YnjI
RA 1,847,969 C→T G14R (GGA→AGA)  selD ← selenide, water dikinase
RA 1,853,057 C→T G308D (GGC→GAC)  ydjE ← putative transporter YdjE
RA 1,853,977 C→T M1M (ATG→ATA) † ydjE ← putative transporter YdjE
RA 1,866,420 C→T G18D (GGC→GAC)  mipA ← MltA‑interacting protein
RA 1,876,099 C→T L143L (CTG→TTG)  nimT → 2‑nitroimidazole exporter
RA 1,878,855 C→T A28V (GCT→GTT)  yoaK → putative membrane protein YoaK
RA 1,881,623 C→T E63K (GAA→AAA)  dmlR ← DNA‑binding transcriptional regulator DmlR
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,906,796 C→T V89M (GTG→ATG)  rlmA ← 23S rRNA m(1)G745 methyltransferase
JC JC 1,909,925 IS5 (+) +4 bp coding (172‑175/792 nt) kdgR ← DNA‑binding transcriptional repressor KdgR
RA 1,940,095 C→T M33I (ATG→ATA lpxM ← Lipid A biosynthesis myristoyltransferase
RA 1,948,824 C→T A567T (GCA→ACA)  aspS ← aspartate‑‑tRNA ligase
RA 1,955,010 G→A D666D (GAC→GAT torZ ← trimethylamine N‑oxide reductase 2
RA 1,969,982 C→T A335T (GCG→ACG)  tap ← methyl‑accepting chemotaxis protein Tap
RA 1,994,637 A→G Y24H (TAC→CAC)  uvrC ← UvrABC excision nuclease subunit C
RA 2,033,271 C→T V51M (GTG→ATG)  drpB ← cell division protein DrpB
RA 2,040,289 C→T A271V (GCG→GTG)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,411 G→A G13S (GGT→AGT)  zinT → metal‑binding protein ZinT
RA 2,046,506 G→T V523V (GTG→GTT yeeJ → inverse autotransporter adhesin
RA 2,064,246 C→T E74E (GAG→GAA cobT ← nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
RA 2,064,616 C→T A203T (GCT→ACT)  cobS ← cobalamin 5'‑phosphate synthase
RA 2,080,405 C→T G63S (GGT→AGT)  yeeA ← putative transporter YeeA
RA 2,082,863 C→T T36T (ACC→ACT sbcB → exodeoxyribonuclease I
RA 2,083,929 C→T P392S (CCG→TCG)  sbcB → exodeoxyribonuclease I
RA 2,084,330 C→T A42T (GCA→ACA)  tsuB ← putative thiosulfate sulfurtransferase
RA 2,085,319 G→A A69A (GCC→GCT tsuA ← thiosulfate transporter
RA 2,086,634 G→A A143A (GCC→GCT plaP ← putrescine:H(+) symporter PlaP
RA 2,103,891 G→A P207S (CCA→TCA)  wbbK ← putative glycosyltransferase WbbK
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,122,908 G→A I24I (ATC→ATT cpsG ← phosphomannomutase
RA 2,134,498 G→A V385V (GTC→GTT wzc ← protein‑tyrosine kinase Wzc
RA 2,137,543 G→A intergenic (‑300/‑359) wza ← / → yegH outer membrane polysaccharide export protein Wza/inner membrane protein YegH
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,174,359 IS5 (+) +4 bp coding (205‑208/285 nt) gatB ← galactitol‑specific PTS enzyme IIB component
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
RA 2,329,209 A→G W197R (TGG→CGG)  yfaQ ← tandem DUF2300 domain‑containing protein YfaQ
RA 2,339,162 C→T D87N (GAC→AAC)  gyrA ← DNA gyrase subunit A
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,510,609 C→T P327L (CCG→CTG)  yfeO → putative transport protein YfeO
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,732,992 G→A I394I (ATC→ATT
I394I (ATC→ATT
clpB ←
clpB ←
chaperone protein ClpB
chaperone protein ClpB
RA 2,789,286 G→A G101D (GGT→GAT)  glaH → glutarate dioxygenase GlaH
RA 2,804,133 C→T S211F (TCT→TTT)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta
RA 2,814,459 Δ1 bp coding (275/516 nt) luxS ← S‑ribosylhomocysteine lyase
RA 2,814,461 C→T L91L (CTG→CTA luxS ← S‑ribosylhomocysteine lyase
RA 2,817,197 G→A R103C (CGC→TGC)  yqaB ← fructose‑1‑phosphate phosphatase YqaB
RA 2,823,288 C→T G161D (GGC→GAC)  recA ← DNA recombination/repair protein RecA
RA 2,867,454 T→A Q33L (CAG→CTG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,871,153 T→G N51T (AAC→ACC)  truD ← tRNA pseudouridine(13) synthase
MC JC 2,904,103 Δ61 bp intergenic (+729/+584) ygcE → / ← queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
RA 2,926,543 2 bp→TT coding (236‑237/1365 nt) ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 3,028,546 G→A A156V (GCC→GTC)  ygfS ← putative electron transport protein YgfS
RA 3,050,472 G→A L66L (CTG→TTG)  gcvT ← aminomethyltransferase
RA 3,061,916 C→T A356V (GCC→GTC)  scpA → methylmalonyl‑CoA mutase
RA 3,065,449 G→A L216L (TTG→TTA scpC → propionyl‑CoA:succinate CoA transferase
RA 3,065,861 G→A V354I (GTC→ATC)  scpC → propionyl‑CoA:succinate CoA transferase
RA 3,089,260 T→C V326A (GTG→GCG)  galP → galactose:H(+) symporter
RA 3,090,968 G→A E208K (GAG→AAG)  endA → DNA‑specific endonuclease I
RA 3,104,887 G→A S152N (AGC→AAC)  mltC → membrane‑bound lytic murein transglycosylase C
RA 3,120,786 G→A T165I (ACT→ATT)  glcA ← glycolate/lactate:H(+) symporter GlcA
RA 3,121,033 T→C I83V (ATT→GTT)  glcA ← glycolate/lactate:H(+) symporter GlcA
RA 3,127,981 G→A P14L (CCC→CTC)  glcD ← glycolate dehydrogenase, putative FAD‑linked subunit
RA 3,130,161 G→A L19F (CTT→TTT)  yghO ← putative DNA‑binding transcriptional regulator YghO
RA 3,132,077 G→A A111V (GCG→GTG)  yghQ ← putative transport protein YghQ
RA 3,133,175 G→A T13I (ACC→ATC)  yghR ← putative ATP‑binding protein YghR
RA 3,134,263 G→A A45T (GCC→ACC)  yghT → putative ATP‑binding protein YghT
RA 3,145,944 G→A L106L (CTC→CTT hybO ← hydrogenase 2 small subunit
RA 3,150,316 G→A E219K (GAG→AAG)  yghA → NADP(+)‑dependent aldehyde reductase
RA 3,162,216 G→A Q152* (CAG→TAG)  ftsP ← cell division protein FtsP
RA 3,176,625 G→A S70F (TCC→TTC)  cpdA ← cAMP phosphodiesterase
RA 3,179,383 G→A K423K (AAG→AAA tolC → outer membrane channel TolC
RA 3,186,297 G→A R37R (CGG→CGA insC5 → IS2 insertion element repressor InsA
RA 3,193,351 G→A G163E (GGG→GAG)  yqiK → flotillin family inner membrane protein YqiK
RA 3,204,617 G→A intergenic (‑28/‑77) folB ← / → plsY dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,219,128 G→A intergenic (‑52/‑366) aer ← / → patA aerotaxis receptor/putrescine aminotransferase
RA 3,224,895 C→T L755L (CTG→TTG)  ebgA → evolved beta‑D‑galactosidase subunit alpha
RA 3,227,339 C→T T369I (ACC→ATC)  ygjI → putative transporter YgjI
RA 3,240,423 C→T G160G (GGC→GGT sstT → serine/threonine:Na(+) symporter
RA 3,256,157 C→T Q165Q (CAG→CAA cyuA ← putative L‑cysteine desulfidase CyuA
RA 3,259,283 G→A H123Y (CAT→TAT)  tdcG ← L‑serine deaminase III
RA 3,270,497 C→T noncoding (96/377 nt) rnpB ← RNase P catalytic RNA component
RA 3,275,902 A→T E207D (GAA→GAT garD → GarD
RA 3,292,312 C→T P324P (CCC→CCT yraK → putative fimbrial adhesin YraK
RA 3,304,647 T→C I391V (ATT→GTT)  mtr ← tryptophan:H(+) symporter Mtr
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,506,393 T→C D315G (GAC→GGC)  yhfT ← uncharacterized protein YhfT
RA 3,518,501 C→T intergenic (‑15/+42) aroB ← / ← aroK 3‑dehydroquinate synthase/shikimate kinase 1
RA 3,524,184 C→T A438A (GCC→GCT mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,558,291 C→T G8G (GGC→GGT rtcR → DNA‑binding transcriptional activator RtcR
RA 3,559,621 C→T P452S (CCC→TCC)  rtcR → DNA‑binding transcriptional activator RtcR
RA 3,560,119 A→G intergenic (+253/+503) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,576,313 A→G V250A (GTG→GCG)  gntU ← low‑affinity gluconate transporter
RA 3,579,050 G→A A199V (GCC→GTC)  yhhW ← quercetin 2,3‑dioxygenase
RA 3,581,316 G→A G60E (GGA→GAA)  yhhY → N‑acetyltransferase YhhY
RA 3,581,850 G→A intergenic (+224/‑13) yhhY → / → yhhZ N‑acetyltransferase YhhY/putative endonuclease YhhZ
RA 3,583,969 G→A W98* (TGG→TAG)  insB6 → IS1 family protein InsB
RA 3,585,517 G→A A436V (GCC→GTC)  ggt ← glutathione hydrolase proenzyme
RA 3,587,531 G→A L195F (CTC→TTC)  ugpQ ← glycerophosphodiester phosphodiesterase UgpQ
RA 3,588,787 G→A P132S (CCG→TCG)  ugpC ← sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
RA 3,589,956 G→A L24L (CTC→CTT ugpE ← sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE
RA 3,590,251 C→T V221M (GTG→ATG)  ugpA ← sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA
RA 3,618,836 C→T V83V (GTC→GTT nikR → DNA‑binding transcriptional repressor NikR
RA 3,621,090 C→T R633R (CGC→CGT rhsB → rhs element protein RhsB
RA 3,633,749 C→T intergenic (‑1159/‑1092) yhiJ ← / → yhiM DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM
RA 3,647,677 C→T intergenic (+26/+28) gor → / ← dinQ glutathione reductase (NADPH)/membrane toxin DinQ
RA 3,684,443 C→T K392K (AAG→AAA pdeK ← c‑di‑GMP phosphodiesterase PdeK
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,713,979 C→T P110S (CCG→TCG)  yiaC → peptidyl‑lysine N‑acetyltransferase YiaC
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,744,369 C→T A14A (GCC→GCT yiaM → 2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
RA 3,781,049 C→T intergenic (+32/‑166) lldD → / → trmL L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
RA 3,803,453 C→T E229K (GAA→AAA)  waaB ← UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
RA 3,803,945 C→T G65S (GGT→AGT)  waaB ← UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
RA 3,809,942 C→T P40S (CCC→TCC)  coaD → pantetheine‑phosphate adenylyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,827,026 C→T A606V (GCG→GTG)  recG → ATP‑dependent DNA helicase RecG
RA 3,829,385 C→T S147S (TCC→TCT xanP → xanthine:H(+) symporter XanP
RA 3,834,845 C→T S362N (AGT→AAT)  yicJ ← putative xyloside transporter YicJ
RA 3,850,507 C→T A74T (GCG→ACG)  uhpA ← DNA‑binding transcriptional activator UhpA
RA 3,870,464 C→T V423M (GTG→ATG)  dgoT ← D‑galactonate:H(+) symporter
RA 3,900,610 C→T W537* (TGG→TGA bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,904,907 C→T E231K (GAA→AAA)  bglF ← beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase
RA 3,914,648 C→T A185T (GCG→ACG)  glmU ← fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase
RA 3,926,894 C→T G37E (GGG→GAG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,927,292 T→C D46D (GAT→GAC asnA → asparagine synthetase A
RA 3,937,909 G→A A206T (GCG→ACG)  rbsK → ribokinase
RA 3,937,997 G→A G235D (GGT→GAT)  rbsK → ribokinase
RA 3,938,441 Δ1 bp coding (215/993 nt) rbsR → DNA‑binding transcriptional dual regulator RbsR
RA 3,941,874 C→T noncoding (67/1542 nt) rrsC → 16S ribosomal RNA
RA 3,946,246 G→A noncoding (2543/2904 nt) rrlC → 23S ribosomal RNA
RA 3,950,099 C→T intergenic (‑130/‑223) yifB ← / → ilvL putative magnesium chelatase YifB/ilvXGMEDA operon leader peptide
RA 3,956,003 G→A D225N (GAT→AAT)  ilvA → threonine deaminase
RA 4,084,932 G→T F297L (TTC→TTA fdoG ← formate dehydrogenase O subunit alpha
RA 4,139,298 G→A A137A (GCC→GCT fsaB ← fructose‑6‑phosphate aldolase 2
RA 4,160,157 G→A L212L (CTG→TTG)  sthA ← soluble pyridine nucleotide transhydrogenase
RA 4,171,728 C→T noncoding (92/120 nt) rrfB → 5S ribosomal RNA
RA 4,171,781 A→G intergenic (+25/‑276) rrfB → / → murB 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase
RA 4,175,827 C→T noncoding (74/76 nt) thrT → tRNA‑Thr
RA 4,178,883 C→T A2V (GCT→GTT)  rplA → 50S ribosomal subunit protein L1
RA 4,179,001 C→T S41S (AGC→AGT rplA → 50S ribosomal subunit protein L1
RA 4,185,777 C→T S143F (TCC→TTC)  rpoC → RNA polymerase subunit beta'
RA 4,193,177 C→T E134K (GAG→AAG)  thiF ← sulfur carrier protein ThiS adenylyltransferase
RA 4,193,707 A→G G166G (GGT→GGC thiE ← thiamine phosphate synthase
RA 4,193,820 C→T G129R (GGA→AGA)  thiE ← thiamine phosphate synthase
RA 4,219,752 C→T S386F (TCC→TTC)  aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,222,743 C→T intergenic (‑256/+61) arpA ← / ← iclR regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,224,483 C→T S219F (TCC→TTC)  metH → cobalamin‑dependent methionine synthase
RA 4,234,479 C→T A241V (GCG→GTG)  pgi → glucose‑6‑phosphate isomerase
RA 4,249,684 C→T A44V (GCT→GTT)  malM → maltose regulon periplasmic protein
RA 4,265,668 C→T T452I (ACC→ATC)  dnaB → replicative DNA helicase
RA 4,269,449 C→T C12C (TGC→TGT aphA → acid phosphatase/phosphotransferase
RA 4,271,130 C→T E914E (GAG→GAA uvrA ← UvrABC excision nuclease subunit A
RA 4,278,488 C→T P4S (CCA→TCA)  ghxP → guanine/hypoxanthine transporter GhxP
RA 4,282,536 C→T Q180Q (CAG→CAA yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,284,077 C→T A276T (GCG→ACG)  actP ← acetate/glycolate:cation symporter
RA 4,286,033 C→T A447T (GCG→ACG)  acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,296,187 C→T intergenic (+393/+249) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,300 C→T intergenic (+506/+136) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,297,469 C→T G633D (GGC→GAC)  fdhF ← formate dehydrogenase H
RA 4,298,199 C→T A390T (GCA→ACA)  fdhF ← formate dehydrogenase H
RA 4,301,071 C→T A670T (GCA→ACA)  mdtO ← putative multidrug efflux pump subunit MdtO
RA 4,303,755 C→T V119I (GTT→ATT)  mdtN ← putative multidrug efflux pump membrane fusion protein
RA 4,311,337 C→T A236T (GCA→ACA)  alsB ← D‑allose ABC transporter periplasmic binding protein
RA 4,320,079 C→T Q195Q (CAG→CAA phnI ← carbon‑phosphorus lyase core complex subunit PhnI
RA 4,320,785 C→T E155K (GAG→AAG)  phnH ← carbon‑phosphorus lyase core complex subunit PhnH
RA 4,322,605 C→T intergenic (‑183/+731) phnF ← / ← phnD putative transcriptional regulator PhnF/phosphonate/phosphate ABC transporter periplasmic binding protein
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,325,837 C→T intergenic (‑96/+562) yjdN ← / ← yjdM PF06983 family protein YjdN/zinc ribbon domain‑containing protein YjdM
RA 4,332,310 C→T G321G (GGG→GGA basS ← sensor histidine kinase BasS
RA 4,337,864 C→T V22V (GTG→GTA adiY ← DNA‑binding transcriptional activator AdiY
RA 4,342,825 C→T N305N (AAC→AAT melA → alpha‑galactosidase
RA 4,349,259 C→T intergenic (‑515/+56) dcuB ← / ← dcuR anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR
RA 4,351,519 C→T T48T (ACG→ACA dcuS ← sensor histidine kinase DcuS
RA 4,351,590 C→T V25I (GTC→ATC)  dcuS ← sensor histidine kinase DcuS
RA 4,359,909 C→T W41* (TGG→TGA cadB ← lysine:cadaverine antiporter
RA 4,364,798 C→T G82D (GGC→GAC)  dsbD ← thiol‑disulfide exchange protein DsbD
RA 4,366,668 C→T A36T (GCC→ACC)  dcuA ← C4‑dicarboxylate transporter DcuA
RA 4,371,401 C→T A126V (GCT→GTT)  groL → chaperonin GroEL
RA 4,373,421 C→T A272T (GCG→ACG)  yjeJ ← protein YjeJ
RA 4,380,469 C→T E17K (GAA→AAA)  frdB ← fumarate reductase iron‑sulfur protein
RA 4,388,120 C→T A417A (GCG→GCA mscM ← miniconductance mechanosensitive channel MscM
RA 4,389,838 C→T V175M (GTG→ATG)  psd ← phosphatidylserine decarboxylase proenzyme
RA 4,401,607 C→T T312T (ACC→ACT hflX → ribosome rescue factor HflX
RA 4,403,010 C→T R325C (CGT→TGT)  hflK → regulator of FtsH protease
RA 4,407,045 C→T A131V (GCT→GTT)  rnr → RNase R
RA 4,414,625 C→T R117R (CGC→CGT aidB → putative acyl‑CoA dehydrogenase AidB
RA 4,418,862 C→T G255D (GGT→GAT)  ulaG ← L‑ascorbate‑6‑phosphate lactonase
RA 4,426,929 C→T C165Y (TGC→TAC)  yjfZ ← DUF2686 domain‑containing protein YjfZ
RA 4,433,459 C→T G189D (GGC→GAC)  qorB ← NAD(P)H:quinone oxidoreductase
JC JC 4,434,629 IS1 (–) +8 bp coding (1930‑1937/1944 nt) cpdB ← 2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase
RA 4,435,124 C→T W481* (TGG→TAG)  cpdB ← 2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase
RA 4,435,750 C→T G272G (GGG→GGA cpdB ← 2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase/3'‑nucleotidase
RA 4,451,280 C→T P75S (CCG→TCG)  ytfR → galactofuranose ABC transporter putative ATP binding subunit
RA 4,457,392 C→T E158E (GAG→GAA yjgA ← putative ribosome biogenesis factor YjgA
RA 4,482,901 C→T V313M (GTG→ATG)  valS ← valine‑‑tRNA ligase
RA 4,486,869 T→A L218M (TTG→ATG)  lptF → lipopolysaccharide transport system protein LptF
RA 4,487,022 C→T R269C (CGT→TGT)  lptF → lipopolysaccharide transport system protein LptF
RA 4,489,775 C→T V97M (GTG→ATG)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,494,499 C→T intergenic (‑93/‑124) idnD ← / → idnK L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive
RA 4,501,500 C→T L81L (CTG→TTG)  yjgZ → uncharacterized protein YjgZ
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,517,355 C→T Q121Q (CAG→CAA fecR ← ferric citrate regulator FecR
RA 4,524,401 C→T G204S (GGC→AGC)  yjhH ← putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase
RA 4,524,596 C→T D139N (GAT→AAT)  yjhH ← putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase
RA 4,529,039 C→T G402D (GGT→GAT)  sgcC ← putative PTS enzyme IIC component SgcC
RA 4,530,797 C→T Q285Q (CAG→CAA sgcX ← putative endoglucanase with Zn‑dependent exopeptidase domain
RA 4,541,261 C→T A102V (GCT→GTT)  fimB → Type 1 fimbriae regulatory protein FimB
JC JC 4,542,042 IS1 (+) +8 bp coding (6‑13/597 nt) fimE → regulator for fimA
RA 4,553,671 C→T F257F (TTC→TTT uxuB → D‑mannonate oxidoreductase
RA 4,564,385 C→T S102N (AGC→AAC)  yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehdratase
RA 4,570,550 C→T S342N (AGC→AAC)  yjiR ← fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
RA 4,571,280 C→T E99K (GAG→AAG)  yjiR ← fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
RA 4,585,673 C→T W363* (TGG→TGA hsdR ← type I restriction enzyme EcoKI endonuclease subunit
RA 4,587,365 C→T S139S (AGC→AGT mrr → Type IV methyl‑directed restriction enzyme EcoKMrr
RA 4,604,517 C→T L120L (CTG→TTG)  bglJ → DNA‑binding transcriptional regulator BglJ
RA 4,630,295 C→T Q76Q (CAG→CAA ettA ← energy‑dependent translational throttle protein EttA
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,058 C→T L455L (CTG→TTG)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 275509 359530 84022 108 [0] [27] 28 [mmuP]–[cynS] [mmuP],mmuM,afuC,insB2,insA2,yagB,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,ykgS,insB3,insA3,yagJ,yagK,yagL,yagM,ykgV,yagN,intF,yagP,paoD,paoC,paoB,paoA,yagU,ykgJ,ecpE,ecpD,ecpC,ecpB,ecpA,matA,ykgL,ykgO,ykgM,ykgR,insE1,insF1,rclC,rclB,rclA,rclR,ykgE,ykgF,ykgG,ykgH,betA,betB,betI,betT,yahV,pdeL,yahB,yahC,yahD,yahE,yahF,yahG,yahH,yahI,yahJ,yahK,yahL,yahM,yahN,yahO,prpR,prpB,prpC,prpD,prpE,codB,codA,cynR,cynT,[cynS]
* * ÷ NC_000913 372333 372748 416 28 [27] [5] 113 mhpD 2‑hydroxypentadienoate hydratase
* * ÷ NC_000913 565829 577187 11359 86 [0] [1] 100 [intD]–nmpC [intD],renD,insE3,insF3,renD,emrE,ylcJ,ybcK,ybcL,ybcM,ylcH,ybcN,ninE,ybcO,rusA,ylcG,quuD,nmpC,insH2,nmpC
* * ÷ NC_000913 3423857–3424435 3424435 1–579 28 [27] [27] 28 [rrlD] [rrlD]
* * ÷ NC_000913 3942234–3943395 3945374–3944045 651–3141 28 [27] [27] 29 [rrsC]–[rrlC] [rrsC],gltU,[rrlC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579072 (0.010)121 (0.930) 100/458 0.3 99.2% intergenic (+8/+16) crl/insB9 RNA polymerase holoenzyme assembly factor Crl/IS1 family transposase B
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl RNA polymerase holoenzyme assembly factor Crl
* ? NC_000913 273955 =NA (NA)86 (0.640) 79/474 0.7 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 565828 0 (0.000)coding (151/1164 nt) intD putative integrase
* ? NC_000913 = 275149NA (NA)99 (0.730) 88/474 0.5 99.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 577188 = 1 (0.010)intergenic (‑363/‑210) nmpC/essD putative outer membrane porin NmpC/putative phage lysis protein
* ? NC_000913 = 2755080 (0.000)108 (0.840) 93/454 0.4 100% coding (184/1404 nt) mmuP S‑methyl‑L‑methionine transporter
?NC_000913 372749 = 0 (0.000)coding (635/810 nt) mhpD 2‑hydroxypentadienoate hydratase
* ? NC_000913 1207790 =69 (0.510)37 (0.290) 36/442 2.5 39.7% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 48 (0.380)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780561 (0.450)63 (0.500) 57/442 1.3 54.6% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 48 (0.380)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)62 (0.470) 56/466 1.5 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785020 (0.000)55 (0.420) 49/458 1.8 100% intergenic (‑305/+16) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA5/uspC IS1 family protein InsA/universal stress protein C