BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,437,890	C→T	Q62* (CAG→TAG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,437,890	0	C	T	98.6%	484.0 / NA	139	Q62* (CAG→TAG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (68/69); minor base G (0/2); total (68/71)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

AATTCAATTGCTAATAAGTTACATTTTAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTC  >  NC_000913/4437595‑4438181

 aTTCAATTGCTAATAAGTTACATTTTAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGAtt                                                                                                                                                                                                                                                                                                <  1:538572/300‑1 (MQ=255)

          cTAATAAGTTACATTTTAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGt                                                                                                                                                                                                                                                                                       >  2:826746/1‑300 (MQ=255)

                 gTTACATTTTAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAGCTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATc                                                                                                                                                                                                                                                                                >  1:583995/1‑300 (MQ=255)

                      aTTTTAAGAATAAGCGTTTTTTTATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATACATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGAGAAAAGTGGTTATCAgg                                                                                                                                                                                                                                                                             <  2:503252/298‑1 (MQ=255)

                       ttttAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTTAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGc                                                                                                                                                                                                                                                                          <  2:156611/300‑1 (MQ=255)

                       ttttAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCTATCATGTATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGACAAAAGTGGTTATCAGGTGc                                                                                                                                                                                                                                                                          >  2:396313/1‑300 (MQ=255)

                       ttttAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGc                                                                                                                                                                                                                                                                          >  1:589388/1‑300 (MQ=255)

                         ttAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTTAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCtt                                                                                                                                                                                                                                                                        <  2:725342/300‑1 (MQ=255)

                         ttAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAAATTAGATTGATAAAAGTGGTTATCAGGTGCtt                                                                                                                                                                                                                                                                        >  2:401880/1‑300 (MQ=255)

                                      ttttttGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAgg                                                                                                                                                                                                                                                                             >  1:498162/1‑282 (MQ=255)

                                      ttttttGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCaaaaaa                                                                                                                                                                                                                                                             >  2:758017/1‑298 (MQ=255)

                                      ttttttGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAAt                                                                                                                                                                                                                                                            >  1:335168/1‑299 (MQ=255)

                                          ttGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAAc                                                                                                                                                                                                                                                       <  2:784203/300‑1 (MQ=255)

                                          ttGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAAc                                                                                                                                                                                                                                                       <  1:158142/300‑1 (MQ=255)

                                                gTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCa                                                                                                                                                                                                                                                 >  1:513940/1‑300 (MQ=255)

                                                    aCTTCTATAGTTAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTCGATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGcc                                                                                                                                                                                                                                             <  1:230315/300‑1 (MQ=255)

                                                    aCTTCTATAGTTAGATATTGAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTGCACGcc                                                                                                                                                                                                                                             <  1:79852/300‑1 (MQ=255)

                                                    aCTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGcc                                                                                                                                                                                                                                             <  1:29535/300‑1 (MQ=255)

                                                     cTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCa                                                                                                                                                                                                                                            >  2:206594/1‑300 (MQ=255)

                                                     cTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCa                                                                                                                                                                                                                                            >  1:343577/1‑300 (MQ=255)

                                                     cTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGNTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCa                                                                                                                                                                                                                                            >  2:678951/1‑300 (MQ=255)

                                                        cTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCAt                                                                                                                                                                                                                                           >  1:443430/1‑298 (MQ=255)

                                                          ataGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGAt                                                                                                                                                                                                                                       >  2:592959/1‑300 (MQ=255)

                                                             gTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAg                                                                                                                                                                                                                                    <  1:137625/300‑1 (MQ=255)

                                                                agaTATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGtt                                                                                                                                                                                                                                  <  2:512931/299‑1 (MQ=255)

                                                                      ttAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATc                                                                                                                                                                                                                           <  2:65690/300‑1 (MQ=255)

                                                                              gACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTCTCTTATCAGTTTCa                                                                                                                                                                                                                    >  2:54833/1‑299 (MQ=255)

                                                                                 aTAAAGTAACCAAATAAAATAAGGTTGTCATATTTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGTAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGcc                                                                                                                                                                                                                 <  2:480768/299‑1 (MQ=255)

                                                                                 aTAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGcc                                                                                                                                                                                                                 <  2:24797/299‑1 (MQ=255)

                                                                                 aTAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTNATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGcc                                                                                                                                                                                                                 <  1:174671/299‑1 (MQ=255)

                                                                                    aaGTAACCACATAAAATAAGGTTGTCATATGTTAACCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACNCATTCCACCAATTTTTTTCACAACATCCATCANGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTaa                                                                                                                                                                                                             >  2:643994/1‑300 (MQ=255)

                                                                                      gTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAg                                                                                                                                                                                                           <  1:519293/300‑1 (MQ=255)

                                                                                       tAACCAAAGAAAATAAGGTTGTCATGTGGTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGa                                                                                                                                                                                                          <  2:39650/300‑1 (MQ=255)

                                                                                        aaCCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTANAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGAt                                                                                                                                                                                                         <  1:162446/300‑1 (MQ=255)

                                                                                        aaCCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGAt                                                                                                                                                                                                         <  1:454729/300‑1 (MQ=255)

                                                                                             aataaaataaGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACgg                                                                                                                                                                                                    <  1:115552/300‑1 (MQ=255)

                                                                                             aataaaataaGGTTGTCATATGCTACCCAGTATCAGACACAATAGTTTTATTGGTGCGGTGGAGTTATGTGTAAAGTCTTTGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTTAGGGAGTTCTTTCGTTGCGCAACTGAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTGTCAGCCGTAAAGATAACgg                                                                                                                                                                                                    <  1:116611/300‑1 (MQ=255)

                                                                                             aataaaataaGGTTGTCAGATGTTACCCAGGATCAGACACAATAATGTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACgg                                                                                                                                                                                                    <  1:432249/300‑1 (MQ=255)

                                                                                                 aaataaGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGcc                                                                                                                                                                                                >  1:80763/1‑300 (MQ=255)

                                                                                                 aaataaGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGcc                                                                                                                                                                                                >  2:474505/1‑300 (MQ=255)

                                                                                                         ttGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATAc                                                                                                                                                                                        <  2:539790/300‑1 (MQ=255)

                                                                                                           gTCATATGTTACCCAGNATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCNGTTTCAGCCTTAAAGATANCGGTGCCGAATATACaa                                                                                                                                                                                      >  1:716720/1‑300 (MQ=255)

                                                                                                           gTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACaa                                                                                                                                                                                      >  2:796541/1‑300 (MQ=255)

                                                                                                           gTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACaa                                                                                                                                                                                      >  1:155615/1‑300 (MQ=255)

                                                                                                           gTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGAGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTCACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTACAGATAACGGTGCCGAATATACaa                                                                                                                                                                                      >  1:357158/1‑300 (MQ=255)

                                                                                                            tCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACGGTTCACGCCATTGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAAt                                                                                                                                                                                     <  2:479544/300‑1 (MQ=255)

                                                                                                              atatGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGa                                                                                                                                                                                   >  1:401495/1‑300 (MQ=255)

                                                                                                                atGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACa                                                                                                                                                                                 >  1:73049/1‑300 (MQ=255)

                                                                                                                 tGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCACCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACAt                                                                                                                                                                                >  2:322133/1‑300 (MQ=255)

                                                                                                                     aCCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGc                                                                                                                                                                             >  1:497944/1‑299 (MQ=255)

                                                                                                                     aCCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAAAGGTGCCGAATATACAATGAGATTGc                                                                                                                                                                             >  2:307817/1‑299 (MQ=255)

                                                                                                                     aCCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAAAGGTGCCGAATATACAATGACATTGc                                                                                                                                                                             >  2:427520/1‑299 (MQ=255)

                                                                                                                      cccAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCNTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCAATGGATAAGTTTCTTATCAGTTCCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGa                                                                                                                                                                           >  2:262913/1‑300 (MQ=255)

                                                                                                                         atgATCAGACACAATAATTTTATTGGTGCGGTGTAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGt                                                                                                                                                                        <  2:163032/298‑1 (MQ=255)

                                                                                                                         aGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAg                                                                                                                                                                          <  2:707182/298‑1 (MQ=255)

                                                                                                                         aGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGt                                                                                                                                                                        <  1:565616/300‑1 (MQ=255)

                                                                                                                         aGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGt                                                                                                                                                                        <  2:556743/300‑1 (MQ=255)

                                                                                                                          ggATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTNTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTa                                                                                                                                                                       >  1:180421/1‑300 (MQ=255)

                                                                                                                           gATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTa                                                                                                                                                                       >  1:298956/1‑299 (MQ=255)

                                                                                                                            aTCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGt                                                                                                                                                                     >  2:380670/1‑300 (MQ=255)

                                                                                                                                gACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCACCTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGAt                                                                                                                                                                 <  2:589388/300‑1 (MQ=255)

                                                                                                                                    caATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGAAATTGCGAGGTAGTGGATTTGa                                                                                                                                                             >  2:560860/1‑300 (MQ=255)

                                                                                                                                          ttttATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACga                                                                                                                                                       >  2:321246/1‑300 (MQ=255)

                                                                                                                                                  gTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAc                                                                                                                                               >  2:22509/1‑300 (MQ=255)

                                                                                                                                                  gTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGCTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATAc                                                                                                                                               >  2:119869/1‑300 (MQ=255)

                                                                                                                                                         ggAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAAc                                                                                                                                        <  1:778617/300‑1 (MQ=255)

                                                                                                                                                            gTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAAt                                                                                                                                     <  2:28636/300‑1 (MQ=255)

                                                                                                                                                                tttGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGGTTTGAATACGAAGAAATACCCATAACAATAAAt                                                                                                                                 >  2:224656/1‑300 (MQ=255)

                                                                                                                                                                      aaGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAatat                                                                                                                           <  2:343577/300‑1 (MQ=255)

                                                                                                                                                                        gTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATaa                                                                                                                         >  1:68462/1‑300 (MQ=255)

                                                                                                                                                                        gTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCACAACAATAAATGAATATaa                                                                                                                         >  1:68799/1‑300 (MQ=255)

                                                                                                                                                                         tCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAAc                                                                                                                        <  1:793833/300‑1 (MQ=255)

                                                                                                                                                                           ttCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGTAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACtc                                                                                                                      <  2:133223/300‑1 (MQ=255)

                                                                                                                                                                                     aaaaCACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGAt                                                                                                            <  1:826746/300‑1 (MQ=255)

                                                                                                                                                                                      aaaCACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCAGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGGATATAACTCACTCATGGAtt                                                                                                           >  2:287465/1‑300 (MQ=255)

                                                                                                                                                                                      aaaCACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAAAGGTGACGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCCTTCATGGact                                                                                                           >  2:287706/1‑298 (MQ=255)

                                                                                                                                                                                           caTTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACCCACTCATGGATTTCaa                                                                                                       >  2:830392/1‑299 (MQ=255)

                                                                                                                                                                                                    actttttttAACAACCTCCATCATGCATTTAAGAAAAAAGATTGAATGTCGAATTATGATAGCCTGTTAACTTGGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACGGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTGAAAGATAACGGTGCCGAATATACAAGGACATTGTGAGTTAGTGGATTGGAATACGAAGAAATACCCATAACAATAAATGAATAGAACtcctgtctcttatacacatctcgcag                                                                                              <  1:643994/297‑25 (MQ=255)

                                                                                                                                                                                                        tttttAACAACATCCATCATGTATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATACATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTc                                                                                         >  2:849258/1‑300 (MQ=255)

                                                                                                                                                                                                                      catcatGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAatat                                                                                                                                                                                          >  1:550539/1‑189 (MQ=255)

                                                                                                                                                                                                                       atcatGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACa                                                                          >  1:369222/1‑300 (MQ=255)

                                                                                                                                                                                                                           tGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACg                                                                      <  2:813762/300‑1 (MQ=255)

                                                                                                                                                                                                                                    gAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGc                                                             <  1:15053/300‑1 (MQ=255)

                                                                                                                                                                                                                                     aaaaaaGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGcc                                                            <  1:206594/300‑1 (MQ=255)

                                                                                                                                                                                                                                                gATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCt                                                 >  1:660789/1‑300 (MQ=255)

                                                                                                                                                                                                                                                      gAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaa                                           >  1:66468/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                  ccTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGCTAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATAc                                                                                                                                                                                        <  2:750832/147‑1 (MQ=255)

                                                                                                                                                                                                                                                                  ccTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATAc                                                                                                                                                                                        >  1:750832/1‑147 (MQ=255)

                                                                                                                                                                                                                                                                  ccTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAAc                                                                                                                        <  1:858812/211‑1 (MQ=255)

                                                                                                                                                                                                                                                                  ccTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAAc                                                                                                                        >  2:858812/1‑211 (MQ=255)

                                                                                                                                                                                                                                                                  ccTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGNGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTAAAGAAAAAACAGTCTGa                               >  2:617934/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                          cTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCATGGGATATTCTACAGAAAAAACAGTCTGACATAcc                         >  1:148067/1‑298 (MQ=255)

                                                                                                                                                                                                                                                                              taGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGACTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                     <  2:583767/297‑1 (MQ=255)

                                                                                                                                                                                                                                                                                 ggAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                <  1:678951/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                  gAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCCTGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAAAGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGACTGGGATATTCTACAGAAAAAACAGTATGAAATCCCGAAAAGGact               >  2:445513/1‑298 (MQ=255)

                                                                                                                                                                                                                                                                                    gTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaa             >  1:841453/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                       cTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt                >  2:135153/1‑294 (MQ=255)

                                                                                                                                                                                                                                                                                             ttGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat    <  1:322133/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                               gcgcAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTc  <  2:542424/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                               gcgcAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTc  <  1:119869/300‑1 (MQ=255)

AATTCAATTGCTAATAAGTTACATTTTAATAATGAGCGTTTTTTGATAGTTTACTTCTATAGTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTC  >  NC_000913/4437595‑4438181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`