Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_012971 2,227,497 2 bp→AA coding (2284‑2285/2628 nt) gyrA ← DNA topoisomerase (ATP‑hydrolyzing) subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0129712,227,4970GA98.8% 298.7 / NA 86P762L (CCA→TTA) gyrADNA topoisomerase (ATP‑hydrolyzing) subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (52/33);  minor base C (1/0);  total (53/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0129712,227,4980GA98.8% 287.1 / NA 86P762L (CCA→TTA) gyrADNA topoisomerase (ATP‑hydrolyzing) subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (52/33);  minor base C (1/0);  total (53/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCCATCGATGGTGTCCAGATCTTCCTCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTGATCGCCACTTTACCGGCAGTACGCAGA  >  NC_012971/2227221‑2227787
                                                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                                                                 
cTGCCATCGATGGTGTCCAGATCTTCCTCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGt                                                                                                                                                                                                                                                                             >  1:687285/1‑300 (MQ=255)
  gCCATCGATGGTGTCCAGATCTTCCTCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGttt                                                                                                                                                                                                                                                                           >  1:190904/1‑300 (MQ=255)
      tCGATGGTGTCCAGATCTTCCTCGGCAACCGGTTCAGCAACTCGTTGCAGACCCACTACGTTTTCAGCGTCCGCAGTACGGATGAGGAGCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGTAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTAcc                                                                                                                                                                                                                                                                        <  2:329479/299‑1 (MQ=255)
            gtgtCCAGATCTTCCTCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCg                                                                                                                                                                                                                                                                 <  1:373035/300‑1 (MQ=255)
            gtgtCCAGATCGTCCTCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAActg                                                                                                                                                                                                                                                                 <  2:651480/300‑3 (MQ=255)
              gtCCAGATCTTCCTCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGtt                                                                                                                                                                                                                                                               >  1:788002/1‑300 (MQ=255)
              gtCCAGATCTTACTCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTAGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGtt                                                                                                                                                                                                                                                               >  2:215195/1‑300 (MQ=255)
                   gaccTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGCTACGGACCACGATGCTGATTCCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGCCATCTACCTGCACAGCGCCAACCACTAAACCGTTACGTTCGGCAACTTTGATGGAAATAACCCATTTCGTCGAACGCGACTCGGTTAAGTATTCCGCCACTGCGGTAAGTTTACCGTAGCCGTTttgc                                                                                                                                                                                                                                                           >  2:8599/4‑299 (MQ=255)
                          cTCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAAACGTTTTGCGTTGCGGt                                                                                                                                                                                                                                                   >  1:418152/1‑300 (MQ=255)
                          cTCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCACACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGt                                                                                                                                                                                                                                                   >  1:416666/1‑300 (MQ=255)
                           tCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGt                                                                                                                                                                                                                                                   >  1:213739/1‑299 (MQ=255)
                           tCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCAATGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGt                                                                                                                                                                                                                                                   >  1:212826/1‑299 (MQ=255)
                                    ggTTCGGCAACGCGTTGCAGACCCACTACTTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGATTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGc                                                                                                                                                                                                                                          <  2:353783/299‑1 (MQ=255)
                                     gTTCAGCAACGCGTTGCCGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGccc                                                                                                                                                                                                                                        >  1:198100/1‑300 (MQ=255)
                                     gTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGccc                                                                                                                                                                                                                                        >  1:79291/1‑300 (MQ=255)
                                      ttCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGccc                                                                                                                                                                                                                                        <  2:400165/299‑1 (MQ=255)
                                            aaCGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGc                                                                                                                                                                                                                                  <  1:142652/299‑1 (MQ=255)
                                                  ttGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCACCCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCATGCGACTTGGTTACGTATTCCGCCACTGCGGTACGTTTAACGTCACCGTTTTGTGTTGCGGTGAGGATTGCCCCCTCGCCAcgc                                                                                                                                                                                                                             >  2:430768/1‑298 (MQ=255)
                                                              tactttttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAgaga                                                                                                                                                                                                               <  2:687285/296‑1 (MQ=255)
                                                                 gTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAAc                                                                                                                                                                                                            >  1:35581/1‑300 (MQ=255)
                                                                            ccGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTANGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTAtcgc                                                                                                                                                                                                 >  1:585994/1‑300 (MQ=255)
                                                                                 gTACGGATGAGGATCAAGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTCAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTCCGATCAGAGAAACGACTTTAtcgccttc                                                                                                                                                                                             >  2:794666/1‑299 (MQ=255)
                                                                                      gATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTaa                                                                                                                                                                                       >  1:144348/1‑300 (MQ=255)
                                                                                      gATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCATTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTaa                                                                                                                                                                                       >  2:279686/1‑300 (MQ=255)
                                                                                      gATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCATTCGCCACGCGGTACGATCAGAGAAACAACTTTATCGCCTTCGCCTaa                                                                                                                                                                                       >  2:279669/1‑300 (MQ=255)
                                                                                      gATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCATTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCAAGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTaa                                                                                                                                                                                       >  1:144333/1‑300 (MQ=255)
                                                                                        tGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAAc                                                                                                                                                                                     <  1:380744/300‑1 (MQ=255)
                                                                                         gAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGTTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACg                                                                                                                                                                                    <  2:508159/300‑1 (MQ=255)
                                                                                           ggATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGa                                                                                                                                                                                  >  1:323480/1‑300 (MQ=255)
                                                                                              tCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATAc                                                                                                                                                                               <  1:746094/300‑1 (MQ=255)
                                                                                              tCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTCAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATAc                                                                                                                                                                               <  1:735960/300‑1 (MQ=255)
                                                                                              tCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGCTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATAc                                                                                                                                                                               <  1:293299/300‑1 (MQ=255)
                                                                                                       gAGTGTTACGGCCCACGATGCTGATTTCCTAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCacac                                                                                                                                                                      <  1:218865/300‑1 (MQ=255)
                                                                                                       gAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTATGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGACACGAATTCATCGACTTCGCATAAACGGATACCGAGCacac                                                                                                                                                                      >  2:20195/1‑300 (MQ=255)
                                                                                                       gAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCGACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCacac                                                                                                                                                                      <  1:218855/300‑1 (MQ=255)
                                                                                                        aGTGTTACGGCCCAAGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCTTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACAcc                                                                                                                                                                     <  2:35581/300‑1 (MQ=255)
                                                                                                                 gCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGtt                                                                                                                                                            >  1:443375/1‑300 (MQ=255)
                                                                                                                   ccACGATGCTGATTTCCGCAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGCATTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGtt                                                                                                                                                            >  2:163986/1‑298 (MQ=255)
                                                                                                                     aCGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATNTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGc                                                                                                                                                          <  1:576032/298‑1 (MQ=255)
                                                                                                                             gATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGc                                                                                                                                                >  1:49805/1‑300 (MQ=255)
                                                                                                                                 tcctaaaCGCGAGTACGCACCAGCGTACCAGCATCGTTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCTTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCacgg                                                                                                                                            <  1:373972/296‑1 (MQ=255)
                                                                                                                                           gAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCTTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGAc                                                                                                                                   <  1:227512/299‑1 (MQ=255)
                                                                                                                                           gAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGAc                                                                                                                                   <  1:227444/299‑1 (MQ=255)
                                                                                                                                                  cACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTaaa                                                                                                                           >  1:511961/1‑300 (MQ=255)
                                                                                                                                                      atcatACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCTCTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGGTCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAgc                                                                                                                         <  1:238359/294‑1 (MQ=255)
                                                                                                                                                      aGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAgcgc                                                                                                                       >  1:685808/1‑300 (MQ=255)
                                                                                                                                                                  tCGGTGATAATCAGGATCTGGTCGCAGTCATCTACTTGCACCGCGCCAACCACTAAACCGTTACGTGCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCTTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCt                                                                                                           <  2:6111/300‑1 (MQ=255)
                                                                                                                                                                                         gCAGTCATCTACCTGCACCGCGCCAACCACTAAACCTTTACGTTCGTTAACCTTGATGTAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCATAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACtt                                                                                     <  2:122544/299‑1 (MQ=255)
                                                                                                                                                                                         gCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATTGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACtt                                                                                     <  2:190904/299‑1 (MQ=255)
                                                                                                                                                                                            gTCATCTACCTGCACCNCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCANCACTTTACCTTCGGCGGANAACAGCATTACTTcatc                                                                                 >  1:664448/1‑297 (MQ=255)
                                                                                                                                                                                              cATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACTACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCtt                                                                               >  1:267219/1‑300 (MQ=255)
                                                                                                                                                                                              cATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGAAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACTACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAACGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCt                                                                                >  1:267924/1‑299 (MQ=255)
                                                                                                                                                                                                 cTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTcgc                                                                            >  2:647403/1‑300 (MQ=255)
                                                                                                                                                                                                  tACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCNGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTcgcc                                                                           >  1:567247/1‑300 (MQ=255)
                                                                                                                                                                                                  tACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCTATCGCACGGACGGAAGACTATTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTcgcc                                                                           >  2:661601/1‑300 (MQ=255)
                                                                                                                                                                                                  tACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTcgc                                                                            >  1:411558/1‑299 (MQ=255)
                                                                                                                                                                                                  tACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGAACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTAGTCTTcgcc                                                                           >  2:312724/1‑300 (MQ=255)
                                                                                                                                                                                                  tACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCATTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAATAGCATTACTTCGTCTTcgcc                                                                           >  2:129871/1‑300 (MQ=255)
                                                                                                                                                                                                   aCCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACTGGTGGTGTTGCAGCCCCTCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTCCGTCTTcgc                                                                            >  2:676556/1‑298 (MQ=255)
                                                                                                                                                                                                            gcgcCAACCACTAAACTGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTCCCGCAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTCCGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTggtcc                                                                   >  2:694579/1‑297 (MQ=255)
                                                                                                                                                                                                            gcgcCAACCACTAAACCGTTACGTTCGTTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTggtcagg                                                                 <  2:769815/300‑1 (MQ=255)
                                                                                                                                                                                                            gcgcCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTTGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTggtca                                                                   >  1:772489/1‑298 (MQ=255)
                                                                                                                                                                                                            gcgcCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGGAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTggtca                                                                   >  2:693712/1‑298 (MQ=255)
                                                                                                                                                                                                                   tcACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGACGATggtcaggtc                                                               >  2:92895/2‑295 (MQ=255)
                                                                                                                                                                                                                   ccACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTACAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGc                                                          >  1:515535/1‑300 (MQ=255)
                                                                                                                                                                                                                   ccACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGc                                                          >  1:515542/1‑300 (MQ=255)
                                                                                                                                                                                                                     attaaacctctacggccgggaacattGATGTAGATAACCCCTTTCGTCGCACGCTACTGGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGTTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCTGAGAACAGCATTACTTCGTCTTCGCCACTGGTCAGGTCAACGc                                                          <  2:150211/274‑1 (MQ=255)
                                                                                                                                                                                                                     aCTAAACCTTTACGTTCGGTAACCTTGATGTAGATAACCCCTTTCGTCGCACGCGACTTGGTGAAGTATTCCGCCACTGTGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCTTCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGc                                                          <  2:296922/298‑1 (MQ=255)
                                                                                                                                                                                                                     aCTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTAGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGc                                                          <  1:430768/298‑1 (MQ=255)
                                                                                                                                                                                                                     aCTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCCCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGc                                                          <  2:783865/298‑1 (MQ=255)
                                                                                                                                                                                                                        aaaCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTCACCTTAGGCGGCGAACAGCATTACTTCGTCTTCGACGCCGGTCAGGTCAACGCAGAt                                                      >  2:394005/1‑299 (MQ=255)
                                                                                                                                                                                                                             gTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTACAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTc                                                >  2:545038/1‑300 (MQ=255)
                                                                                                                                                                                                                                 cGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATc                                             >  1:121527/1‑299 (MQ=255)
                                                                                                                                                                                                                                  gTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGc                                           >  1:714625/1‑300 (MQ=255)
                                                                                                                                                                                                                                           ccTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTccacaa                                  >  1:129680/1‑295 (MQ=255)
                                                                                                                                                                                                                                           ccTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCa                                  >  1:547755/1‑300 (MQ=255)
                                                                                                                                                                                                                                              tgagggAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGtt                               <  1:338994/296‑1 (MQ=255)
                                                                                                                                                                                                                                                aTGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGTTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTg                             <  1:279669/300‑1 (MQ=255)
                                                                                                                                                                                                                                                aTGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTg                             <  1:279686/300‑1 (MQ=255)
                                                                                                                                                                                                                                                   gagaTAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTGAt                           >  2:657292/1‑299 (MQ=255)
                                                                                                                                                                                                                                                    agaTAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGTACACCGGTGGTGTTGCAGCCCATCGCACGGAAGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGNCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTGATc                          >  1:171714/1‑299 (MQ=255)
                                                                                                                                                                                                                                                    agaTAACCCCTTTCGTCGCACGCAACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTCCTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTGAt                           >  2:448196/1‑298 (MQ=255)
                                                                                                                                                                                                                                                     gaTAACCCCTTTCGTCGCACGCGACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTGATCGc                        >  1:76106/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                          cgACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTGATCGCCACTTTACCGGCAGTACGCa    >  1:652323/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                           taCTTGGTTAAGTATTCCTCCACTTCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCTGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTGATCGCCACTTTACCGGCAGTACGCAGa  <  2:800010/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gACTTGGTTAAGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTGATCGCCACTTTACCGGCAGTACGCAGa  <  1:215195/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                                                                                 
CTGCCATCGATGGTGTCCAGATCTTCCTCGTCAACCGGTTCAGCAACGCGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGAGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGCACCAGCGTACCAGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGCACCGCGCCAACCACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCCCCTTCGCCACGCGGTACGATCAGAGAAACGACTTTATCGCCTTCGCCTAAACGGATACCGCGCACACCGGTGGTGTTGCAGCCCATCGCACGGACGGAAGACTCTTTAAAGCGCACCACTTTACCTTCGGCGGAGAACAGCATTACTTCGTCTTCGCCGCTGGTCAGGTCAACGCCGATCAGCTCATCGCCTTCAACCAGTTTGATCGCCACTTTACCGGCAGTACGCAGA  >  NC_012971/2227221‑2227787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑