Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012971	1,173,770	G→A	L930F (CTT→TTT)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012971	1,173,770	0	G	A	100.0%	192.2 / NA	55	L930F (CTT→TTT)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/34);  total (21/34)

GCGTGTTCACGTCAGGGATGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGATGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGC  >  NC_012971/1173491‑1174052                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           gcgTGTTCACGTCAGGGATGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGATGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAg                                                                                                                                                                                                                                                                        <  1:685506/300‑1 (MQ=255)                           tcttgCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGATGGCTGGGGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTACATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGc                                                                                                                                                                                                                                              <  2:134169/296‑1 (MQ=255)                             tgacAATAGCGACGACATCTGCAACTCGACTTCGGTTTGATGGCTGGTGAAAGCTTCCAGCGACGACTCGCATCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCATGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCa                                                                                                                                                                                                                                             <  2:34631/296‑1 (MQ=255)                             tGGCAATATCGACGGCATCCGCAGCTCGACTTCTTTTTGATGGCTGTTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAAAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGGAGTCATCGCTTTTGGATGCGGTGTCAGCa                                                                                                                                                                                                                                             <  2:180087/299‑1 (MQ=255)                               gCAATAGCAACTGCATCCGCAGCTCGACTTCTGTTTGATGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCTGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCTATGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCaaca                                                                                                                                                                                                                                             <  2:35091/297‑4 (MQ=255)                                    aGCGATGGCAGCCGCAGCTCGACTTCTGTTTGATGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGGTTTCCAGCAACGCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCa                                                                                                                                                                                                                                     <  2:94731/300‑1 (MQ=255)                                     gCGACGGCATCCGCAGCTCGACTTCTGTTTGATGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCat                                                                                                                                                                                                                                    <  1:697373/300‑1 (MQ=255)                                     gCGACGGCATCCGCAGCTCGACTTCTGTTTGATGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTAGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCat                                                                                                                                                                                                                                    >  2:754808/1‑300 (MQ=255)                                           gCATCCGCAGCTCGACTTCTGTTTGATGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAGCCGATGGTGTCCATTGAGCCGCTTTGTTCTCCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTTCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGGGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCa                                                                                                                                                                                                                                             <  2:32831/285‑1 (MQ=255)                                               ccGCAGCTCGACTTCTGTTTGATGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCtgat                                                                                                                                                                                                                           >  1:636324/1‑299 (MQ=255)                                                               gTTTGATGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCAAGCGAGGCAATCGATTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCATGATGATGCGAACGTCCGAc                                                                                                                                                                                                           >  2:126108/1‑299 (MQ=255)                                                                            tGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAAc                                                                                                                                                                                              <  1:19612/299‑1 (MQ=255)                                                                            tGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTCTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAAc                                                                                                                                                                                              <  2:545152/299‑1 (MQ=255)                                                                             gagaTCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTg                                                                                                                                                                                            <  2:487885/300‑1 (MQ=255)                                                                              agaTCTTCCAGCGACGGCTCGCGTCCGGCTGTCAGTGCATCGACGGCGTTTTCCAGCAACTCCGTATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCTAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTgg                                                                                                                                                                                           <  2:421584/300‑1 (MQ=255)                                                                              agaTCTTCCAGCGACGGCTCGCGTCCGGCCTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTgg                                                                                                                                                                                           <  2:421439/300‑1 (MQ=255)                                                                                       aGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTg                                                                                                                                                                                   <  2:336380/299‑1 (MQ=255)                                                                                                    gTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAg                                                                                                                                                                      <  1:555096/299‑1 (MQ=255)                                                                                                        tgCTTTCAGTACATCGACGGCTTTTTCCAGCAACTCCATATACAGCTAGAAACCGATGGTTTCCATTTAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATc                                                                                                                                                                 <  2:403791/299‑1 (MQ=255)                                                                                                        ggCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATc                                                                                                                                                                 <  1:401173/300‑1 (MQ=255)                                                                                                        ggCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATc                                                                                                                                                                 <  1:401164/300‑1 (MQ=255)                                                                                                                gTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCat                                                                                                                                                                                                                                    <  1:237860/225‑1 (MQ=255)                                                                                                                gTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAAAGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGAACGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGtt                                                                                                                                                         >  2:190787/1‑300 (MQ=255)                                                                                                                gTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTANAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCat                                                                                                                                                                                                                                    >  2:237860/1‑225 (MQ=255)                                                                                                                                     gCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCTAGGCAATCGCTTCAAAACGTTTTTTTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTg                                                                                                                                    <  2:535245/300‑1 (MQ=255)                                                                                                                                         cTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCNAAACCTGCCCCGAGATCTTCCAGCGAGGCAATNGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTgg                                                                                                                                       >  2:602592/1‑293 (MQ=255)                                                                                                                                         cTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGTTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTTTTgg                                                                                                                                       >  2:446625/1‑293 (MQ=255)                                                                                                                                          tCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCggg                                                                                                                               <  1:446625/300‑1 (MQ=255)                                                                                                                                           ccATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTTTCCGCCAAGCAGTTCACCCACGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCACCGAGATCTTCAAGCGAGGCAATCGATTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCCACCATGCATATGTCTGATGATGCGAACGTCCGACGCGACCGCGCAACt                                                                                                                                                                                             >  2:230541/1‑237 (MQ=255)                                                                                                                                           ccATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGa                                                                                                                              >  1:53553/1‑300 (MQ=255)                                                                                                                                                    gCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATc                                                                                                                      >  2:146439/1‑299 (MQ=255)                                                                                                                                                                 gatttCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGACTCTCCAGATCGTGCGTTACCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTAAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCATGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGTGATGTCGATCCCGGTTTCGATAAt                                                                                                        >  2:328298/3‑300 (MQ=255)                                                                                                                                                                   tttCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAAtgg                                                                                                      <  1:69750/300‑1 (MQ=255)                                                                                                                                                                        aTTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTa                                                                                                 <  1:693823/300‑1 (MQ=255)                                                                                                                                                                        aTTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTa                                                                                                 <  1:693852/300‑1 (MQ=255)                                                                                                                                                                                gCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCa                                                                                          >  1:224439/1‑299 (MQ=255)                                                                                                                                                                                   ttGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCAc                                                                                      <  1:292644/300‑1 (MQ=255)                                                                                                                                                                                   ttGTTCTTCGCCAAGCAGTTCACCCACGCCGCGAATCTCCAGATCGTGCTGTGCCAGCGCAAAACCTGCCCCGAGATCTTTCAGCTATGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAATCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACGGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCAc                                                                                      <  2:16107/300‑1 (MQ=255)                                                                                                                                                                                   ttGTGCTTCGCCAAGCAGTTCACCCCCGCCGCGAATCTCCAGATCGTTCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCTGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCAc                                                                                      <  2:14828/300‑1 (MQ=255)                                                                                                                                                                                          tcGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTNGTGCATCGGTAGTCATCGCTTTTGGATGCGGTNTCAGCAACCATGCATATGCCTGATGATGCGAATGTCTGAAGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTGCGATAATGGTGGTACAAACCAGCACGTTGa                                                                                 >  2:593663/1‑298 (MQ=255)                                                                                                                                                                                                    gTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTgatgat                                                                     >  1:739944/1‑300 (MQ=255)                                                                                                                                                                                                                      tctcCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGNAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAAACAGCACGTTGAAACGTTGATGATGGAAATCATTCATCAccc                                                   >  2:241564/1‑300 (MQ=255)                                                                                                                                                                                                                      tctcCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCAccc                                                   >  1:759677/1‑300 (MQ=255)                                                                                                                                                                                                                        tcCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCTAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCg                                                  <  1:415395/299‑1 (MQ=255)                                                                                                                                                                                                                           aGATCGTGCGTTGCCAGCGCAAAACCTGCCCCTAGATCTTCCAGCTAGGCAATCGCTTCAAAACTTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTcc                                              <  2:685915/300‑1 (MQ=255)                                                                                                                                                                                                                           aGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTcc                                              <  2:543048/300‑1 (MQ=255)                                                                                                                                                                                                                                gTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGtt                                          <  2:739944/299‑1 (MQ=255)                                                                                                                                                                                                                                gTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTtc                                         >  1:317434/1‑300 (MQ=255)                                                                                                                                                                                                                                 tGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTAGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTtc                                         >  2:473107/1‑299 (MQ=255)                                                                                                                                                                                                                                     ttGCCAGCGCAAAACCTNCCCCGAGATCTTCCAGCGNGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACNCGTTCCAGTtcgcgct                                    <  1:661149/300‑1 (MQ=255)                                                                                                                                                                                                                                            cgcAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCAt                             >  1:629364/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTNAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGCAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCATCTACACGTGa                   >  2:257470/1‑300 (MQ=255)                                                                                                                                                                                                                                                       ccccGAGATCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCAGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCATCTGCCCGTGAc                  >  2:391925/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gagaTCTTCCAGCGAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCATCTGCCCGTGAc                  <  1:87857/296‑1 (MQ=255)                                                                                                                                                                                                                                                                        gAGGCAATCGCTTCAAAACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCgcgc  <  1:754808/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           GCGTGTTCACGTCAGGGATGAAATCATCTGGCAATAGCGACGGCATCCGCAGCTCGACTTCTGTTTGATGGCTGGTGAGATCTTCCAGCGACGGCTCGCGTCCGGCTTTCAGTGCATCGACGGCGTTTTCCAGCAACTCCATATACAGCGAGAAACCGATGGTTTCCATTGAGCCGCTTTGTTCTTCGCCAAGCAGTTCACCCGCGCCGCGAATCTCCAGATCGTGCGTTGCCAGCGCAAAACCTGCCCCGAGATCTTCCAGCGAGGCAATCGCTTCAAGACGTTTTTGTGCATCGGTAGTCATCGCTTTTGGATGCGGTGTCAGCAACCATGCATATGCCTGATGATGCGAACGTCCGACGCGACCGCGTAACTGGTGCAGCTGCGCCAGACCGAAGTGATCCGCCCGTTCAATGATAATGGTGTTGGCTGTCGGGATGTCGATCCCGGTTTCGATAATGGTGGTACAAACCAGCACGTTGAAACGTTGATGATGGAAATCATTCATCACCCGTTCCAGTTCGCGCTCGCGCATCTGCCCGTGACCGATGGCGATCCGCGC  >  NC_012971/1173491‑1174052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑