Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012971	1,100,143	G→A	noncoding (76/88 nt)	ECD_RS05485 ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012971	1,100,143	0	G	A	98.4%	188.5 / NA	62	noncoding (76/88 nt)	ECD_RS05485	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (39/22);  total (40/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCTTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAATGC  >  NC_012971/1099869‑1100437                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTCAATATGGCGGTGAGGGAGGGATTCGACCCCCCAATACGtcgc                                                                                                                                                                                                                                                                               >  1:709869/1‑297 (MQ=255)      gggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTAGTACGAGCCCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTCCGCTCGCCCTACGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCAAGTTGTCGACCCCCGGTCTGGGCTTCTCATCCACCCA‑GGTGTGTGCAATATACGAAAAAAAA‑ACACGTACTTTTGTACGGACCTTTCTTTAACTATGGCGGTGAGAGATAGATTCGAACACCAGATACGTTGctgtgt                                                                                                                                                                                                                                                                          >  2:677191/1‑295 (MQ=21)        gcgtcttgtCCCCCCGGTGTGGGCAGTCTACGAAAAGAAAGCCCGTACTTTCGTACGAGCTCTGCTTTAAATATGTTGGTGAGGGGGGGAGTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAGTATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATac                                                                                                                                                                                                                                                                        <  2:532281/292‑1 (MQ=21)        gCTTCTCATCCCCCCGGTGTTTGCAGTAGACTAAAAAAAGACCTGTACTTTCGTAAGAGCGCTTCTTTAAATATGTCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGTTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATac                                                                                                                                                                                                                                                                        <  1:298827/300‑1 (MQ=21)         cTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATaca                                                                                                                                                                                                                                                                       >  1:703216/1‑300 (MQ=255)           tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAAAGCCAGTACTTTAGCAAGGGTTTTTTTTTAAGCTGGGGGGTGGGGGGGGGGTTCGAAccccaactacttttcagcgccccc                                                                                                                                                                                                                                                                      >  1:499370/1‑280 (MQ=18)                tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATCCTTTGCCGTATACACACTTTc                                                                                                                                                                                                                                                                >  2:477298/1‑300 (MQ=255)                  cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTAAGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTAGAACCCCCGATACGTTGCCGTATACACACTTTCCa                                                                                                                                                                                                                                                              >  1:451819/1‑300 (MQ=255)                       ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGANTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAgg                                                                                                                                                                                                                                                            <  1:584026/297‑1 (MQ=255)                       ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCGTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCg                                                                                                                                                                                                                                                          <  1:484169/299‑1 (MQ=255)                       ggTGTGTGCAATATACGAAAAAAAAGCCCATACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCg                                                                                                                                                                                                                                                          <  1:334355/299‑1 (MQ=255)                        gtgtgtGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCTGTGATGGGGGGATTGACTCGCTTCGCTCGCCCTGCGTGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGTTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACGTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACt                                                                                                                                                                                                                                                                   <  2:51486/289‑1 (MQ=255)                          gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGNGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCt                                                                                                                                                                                                                                                      >  1:155712/1‑300 (MQ=255)                            gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATGGACTCGCTTCGCTCGCCCTGCGGGCAACCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTcc                                                                                                                                                                                                                                                    <  1:299747/300‑1 (MQ=255)                                           aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGac                                                                                                                                                                                                                                     <  1:697397/300‑1 (MQ=255)                                             aaaGCCCGGACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTTCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGacac                                                                                                                                                                                                                                   <  2:914/300‑1 (MQ=255)                                                         ttCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGAGTGACTCGCTTCACTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGTTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACg                                                                                                                                                                                                                                                                                   <  2:129366/240‑1 (MQ=255)                                                          tCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACNGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGt                                                                                                                                                                                                                      <  1:171092/300‑1 (MQ=255)                                                                gAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGANCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTcc                                                                                                                                                                                                                >  1:583486/1‑300 (MQ=255)                                                                         tttAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTG‑GGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTg                                                                                                                                                                                                      <  1:481495/300‑1 (MQ=255)                                                                            aaaTATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGa                                                                                                                                                                                                    >  1:87459/1‑300 (MQ=255)                                                                                   gcgttggggaagggcatGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTTGCTGTCGCCAGTTGTCGACCCCCGTTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGacac                                                                                                                                                                                                                                   <  2:321860/246‑1 (MQ=18)                                                                                      gTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTa                                                                                                                                                                                          >  1:84364/1‑300 (MQ=255)                                                                                               ggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAAACCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGTGCTAATTTAGGGa                                                                                                                                                                                 >  2:341216/1‑300 (MQ=255)                                                                                               ggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCATTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGa                                                                                                                                                                                 >  2:341957/1‑300 (MQ=255)                                                                                                                             aGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCCCGGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAACTGAGGTCAACAAACttttt                                                                                                                                                    >  1:410077/1‑300 (MQ=255)                                                                                                                             aGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCAA‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtttttt                                                                                                                                                   >  1:408692/1‑300 (MQ=255)                                                                                                                                       cTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATc                                                                                                                                           >  1:136526/1‑298 (MQ=255)                                                                                                                                         gCGTTCACGGTCTGTCCAACCGGCTTTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAgg                                                                                                                                                                                                                                                            <  2:731956/183‑1 (MQ=255)                                                                                                                                          cGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATcgcgc                                                                                                                                       >  1:534170/1‑299 (MQ=255)                                                                                                                                           gTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCACGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGt                                                                                                                                     >  1:263588/1‑300 (MQ=255)                                                                                                                                                  gTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATNGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCa                                                                                                                              >  2:627877/1‑300 (MQ=255)                                                                                                                                                    cTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCaaa                                                                                                                            >  2:671572/1‑300 (MQ=255)                                                                                                                                                      gTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGNTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTCAAAAATCGCGCGTTTATTCAAACt                                                                                                                          >  1:155587/1‑300 (MQ=255)                                                                                                                                                      gTCCAACCGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGCGTGCAATATCCGAAAAAAAA‑GTCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGACCCCCCGCTCCGTTGCCGTATACCCACTTTCCCGGCGTGCTCCTTCAGCCACTAGGACCCCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGATAATTTAGGGAAAt                                                                                                                                                                              >  1:144982/1‑248 (MQ=255)                                                                                                                                                                 tGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAatat                                                                                                               >  1:430107/1‑300 (MQ=255)                                                                                                                                                                  gTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGGTTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAAAAAACTTTTTTAAGAAATCGAGCGTTTATTCAAACTTCAATCAatat                                                                                                               >  2:129497/1‑299 (MQ=255)                                                                                                                                                                             tctaCCCCCGTTCGGTGCTTCTCCTCCCCCCC‑GGTGTGTGCAATATACTAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCATGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAg                                                                                                           <  2:337700/289‑1 (MQ=255)                                                                                                                                                                             tCGAACCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTgaata                                                                                                   <  1:746348/300‑1 (MQ=255)                                                                                                                                                                                              cTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGACCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCACATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTCTTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCttttt                                                                                  >  1:504516/1‑300 (MQ=255)                                                                                                                                                                                              cTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTACTAAGCGAAATCTGCttttt                                                                                  >  1:145993/1‑300 (MQ=255)                                                                                                                                                                                              cTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCACGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGTCACTCGGACACCTCACCAAATTNTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCACTATGCAGTTTTAATAAGCGAAATCTGCttttt                                                                                  >  2:239831/1‑300 (MQ=255)                                                                                                                                                                                                   cctCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGCACCCCCGATACGTTGCAGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCACTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGAGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTACTTTTTTTTGc                                                                             >  1:703645/3‑300 (MQ=255)                                                                                                                                                                                                     tccccccggtgtgtgtGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGc                                                                             >  1:701915/11‑299 (MQ=255)                                                                                                                                                                                                       cccccc‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTAAGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCAGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACATGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTtaata                                                                                                   >  2:119020/1‑274 (MQ=255)                                                                                                                                                                                                        ccccc‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCGCTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCAcc                                                                         >  2:543783/1‑299 (MQ=255)                                                                                                                                                                                                        ccccc‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCCCTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCAcc                                                                         >  2:543796/1‑299 (MQ=255)                                                                                                                                                                                                        ccccc‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCAcc                                                                         >  1:130181/1‑299 (MQ=255)                                                                                                                                                                                                        ccccc‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAACTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCaac                                                                         >  1:128391/1‑297 (MQ=255)                                                                                                                                                                                                        ccccc‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCAACAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCAcc                                                                         >  2:394421/1‑299 (MQ=255)                                                                                                                                                                                                        cccac‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCCTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCCACAAACTTTTTTCAAAAATCACGAGTTTATTCAAACTTCCATCAATATGCAGttttcatcagc                                                                                                >  2:188423/5‑269 (MQ=25)                                                                                                                                                                                                              ggTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCg                                                                                                                                                                                                                                                          <  2:91013/117‑1 (MQ=255)                                                                                                                                                                                                              ggTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGAc                                                                      <  1:526616/297‑1 (MQ=255)                                                                                                                                                                                                              ggTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCAc                                                                   <  1:234680/300‑1 (MQ=255)                                                                                                                                                                                                                 gtgtgCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGa                                                                <  1:477298/300‑1 (MQ=255)                                                                                                                                                                                                                 gtgtgCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCAACGACCACGGa                                                                >  1:800951/1‑300 (MQ=255)                                                                                                                                                                                                                 gtgtgCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCAACAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATTTGCTTTTTTTTGCAACCGACCACGGa                                                                >  2:472891/1‑300 (MQ=255)                                                                                                                                                                                                                                          ccGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTAAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGAACACGGATTTGTTATGCTGGTGGATTTTGTa                                        >  2:459356/1‑300 (MQ=255)                                                                                                                                                                                                                                               cTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCTTTTGTAGATCa                                   >  2:764264/1‑300 (MQ=255)                                                                                                                                                                                                                                                     tACGTGCTTTTTTTTAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCTTTTGTAGATCATAAc                               <  2:758694/298‑1 (MQ=255)                                                                                                                                                                                                                                                            ttttttttAAATATGGCGGTGAGGGAGGGATTCGAACCCCCGATACGTCGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCTTTTGTAGATCATAACGATAAGTg                       <  2:709869/299‑1 (MQ=255)                                                                                                                                                                                                                                                                              gTGAGGGAGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCAAGGATTTGTTATGCTGGTGGCTTTTGTAGATCATAACAATAAATGCGAATAAATTTCGCACAAt    >  2:668619/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                gagggaggGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGNGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACTACGGATTTGTTATGCTGGTGGCTTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAATGc  >  2:255277/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       GGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCC‑GGTGTGTGCAATATACGAAAAAAAA‑GCCCGTACTTTCGTACGGGCTTTTTTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTTAAAAAATCGCGCGTTTATTCAAACTTCAATCAATATGCAGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCTTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAATGC  >  NC_012971/1099869‑1100437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑