BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012971	762,873	T→C	D171D (GAT→GAC)	ECD_RS03820 →	replication protein P

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012971	762,873	0	T	C	99.1%	399.5 / NA	108	D171D (GAT→GAC)	ECD_RS03820	replication protein P
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (48/59); minor base A (1/0); total (49/59)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

ACGGGATCACCACGATGGAACAGGTTAACGCAGGAATGCGCGTAGCCCGTCGGCAGAATCGACCATTTCTGCCATCACCCGGGCAGTTTGTTGCATGGTGCCGGGAAGAAGCATCCGTTACCGCCGGACTGCCAAACGTCAGCGAGCTGGTTGATATGGTTTACGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCC‑ATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAACCAGCATAAATC  >  NC_012971/762590‑763161

gtttcccggtgagtgggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaacagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgggggccaaTGCGCTGACTGACGCGGAattacggcgca                                                                                                                                                                                                                                                                                   >  2:232902‑M2/272‑295 (MQ=255)

              gggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTAAGGCGCAAGGCTGCCGATgaa                                                                                                                                                                                                                                                                     >  2:748674‑M2/258‑299 (MQ=255)

               ggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGAtgaac                                                                                                                                                                                                                                                                    <  2:674586‑M2/44‑3 (MQ=255)

               ggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGAtgaac                                                                                                                                                                                                                                                                    >  2:55683‑M2/257‑298 (MQ=255)

               ggttaataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGAtgaac                                                                                                                                                                                                                                                                    <  1:229070‑M2/44‑3 (MQ=255)

               ggttaataaatgccagtcgtcctgccgtgatgaatctgaactcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGAtgaac                                                                                                                                                                                                                                                                    >  2:54776‑M2/257‑298 (MQ=255)

                    ataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctntatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTgccgatgaactgtcc                                                                                                                                                                                                                                                               <  2:571209‑M2/49‑8 (MQ=255)

                    ataaatgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtggcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctntatggtggattcaaccagacataaccggaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTgccgatgaactgtcc                                                                                                                                                                                                                                                               <  2:571223‑M2/49‑8 (MQ=255)

                         tgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAaggctgccgatgaactgtcctgtat                                                                                                                                                                                                                                                          <  1:716183‑M2/54‑13 (MQ=255)

                         tgccagtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcagggaataacattacacatcccacactgctgaatttgttttcagtggctgccnttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAaggctgccgatgaactgtcctgtat                                                                                                                                                                                                                                                          <  2:262964‑M2/54‑13 (MQ=255)

                             agtcgtcctgccttgatgaatctgacctcactggcggttggtatagcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctgtttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcacatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                       <  1:762977‑M2/57‑16 (MQ=255)

                             agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                       <  2:794286‑M2/57‑16 (MQ=255)

                             agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                       <  1:92129‑M2/57‑16 (MQ=255)

                             agtcgtcctgccgtgatgaatctgacctcactggcggtttgtatagcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatgaactgtcctgtatgac                                                                                                                                                                                                                                                       <  1:762970‑M2/57‑16 (MQ=255)

                             agtcgtcctgccgtgangaatctgacctcactggcngtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCgcaaggctgccgatnaactgtcctgtatgac                                                                                                                                                                                                                                                       <  1:671417‑M2/57‑16 (MQ=255)

                                 gtcctgccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTacggcgcaaggctgccgatgaactgtcctgtatgaccgcgc                                                                                                                                                                                                                                                  >  1:195668‑M2/239‑280 (MQ=255)

                                      gccgtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACggcgcaaggctgccgatgaactttcctgtatgaccgcgcgaatt                                                                                                                                                                                                                                             >  1:595086‑M2/234‑279 (MQ=255)

                                         gtgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAAc                                                                                                                                                                                                                                         >  1:559608‑M2/231‑300 (MQ=255)

                                          tgatgaatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcaaaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAAcc                                                                                                                                                                                                                                        >  2:504824‑M2/230‑300 (MQ=255)

                                               aatctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCgtggt                                                                                                                                                                                                                                   >  1:687667‑M2/225‑300 (MQ=255)

                                                atctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaagtggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCgtggtg                                                                                                                                                                                                                                  <  1:132520‑M2/77‑1 (MQ=255)

                                                atctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCgtggtg                                                                                                                                                                                                                                  <  2:740982‑M2/77‑1 (MQ=255)

                                                atctgacctcactggcggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCgtggtg                                                                                                                                                                                                                                  <  1:130144‑M2/77‑1 (MQ=255)

                                                                ggtttgtatcgcttcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatccaacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAAcc                                                                                                                                                                                                                  >  1:754731‑M2/208‑300 (MQ=255)

                                                                        tcgcttcatgaaaccatcctacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggntggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAaac                                                                                                                                                                                                          <  2:576530‑M2/101‑1 (MQ=255)

                                                                             tcatgaaaccatccgacagatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACtt                                                                                                                                                                                                     >  1:540639‑M2/195‑300 (MQ=255)

                                                                                              agatgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggngaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAg                                                                                                                                                                                    <  1:568177‑M2/123‑1 (MQ=255)

                                                                                                 tgtgtcagcagttaataacattacacatcccacactgctgaatttgttttcagttgctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAcc                                                                                                                                                                                 <  1:391132‑M2/126‑1 (MQ=255)

                                                                                                 tgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTNCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAcc                                                                                                                                                                                 >  1:169414‑M2/175‑300 (MQ=255)

                                                                                                 tgtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAcc                                                                                                                                                                                 <  1:391133‑M2/126‑1 (MQ=255)

                                                                                                  gtgtcagcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACct                                                                                                                                                                                >  1:218941‑M2/174‑300 (MQ=255)

                                                                                                        gcagttaataacattacacatcccacactgctgaattttttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAAt                                                                                                                                                                          <  2:668521‑M2/133‑1 (MQ=255)

                                                                                                        gcaggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttacgggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAAt                                                                                                                                                                          <  2:298712‑M2/133‑1 (MQ=255)

                                                                                                           ggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggtatatccaattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGTGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGTGGTAGACCTCTACATCGt                                                                                                                                                                       >  2:3798‑M2/165‑300 (MQ=255)

                                                                                                           ggtaataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGAAGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGt                                                                                                                                                                       >  2:472531‑M2/165‑300 (MQ=255)

                                                                                                               ataacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatgntggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGcac                                                                                                                                                                   <  1:575926‑M2/140‑1 (MQ=255)

                                                                                                               ataacattacacatcccacactgctgaatttgttttcagtggctgccttttgcacaaaaggggaaatattgctgtatggtggatgcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGcac                                                                                                                                                                   <  2:646124‑M2/140‑1 (MQ=255)

                                                                                                                taacattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGcac                                                                                                                                                                   >  1:57940‑M2/160‑299 (MQ=255)

                                                                                                                  acattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAcc                                                                                                                                                                                 <  2:691231‑M2/126‑1 (MQ=255)

                                                                                                                  acattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAcc                                                                                                                                                                                 >  1:691231‑M2/158‑283 (MQ=255)

                                                                                                                  acattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGcaca                                                                                                                                                                  >  1:9164‑M2/158‑298 (MQ=255)

                                                                                                                  acattacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGcaca                                                                                                                                                                  >  2:473659‑M2/158‑298 (MQ=255)

                                                                                                                     ttacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTTAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGctc                                                                                                                                                             <  1:86049‑M2/146‑1 (MQ=255)

                                                                                                                     ttacacatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGctc                                                                                                                                                             <  1:510983‑M2/146‑1 (MQ=255)

                                                                                                                         acatcccacactgctgaatttgttttcagtggctgcctttttcacaaaatgggaaatattgctgtatggtggattcaaccagacataaccagatgcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTTAGGCGATACCTGAACCAGTAAAACAACTGCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGc                                                                                                                                                         <  2:214931‑M2/150‑1 (MQ=255)

                                                                                                                         acatcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCGGGc                                                                                                                                                         <  1:560713‑M2/150‑1 (MQ=255)

                                                                                                                         acaccccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGc                                                                                                                                                         <  2:711669‑M2/150‑1 (MQ=255)

                                                                                                                          catccaacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGc                                                                                                                                                         >  1:416888‑M2/150‑299 (MQ=255)

                                                                                                                          catcacacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaacagaggcatatcccattgcttactggttggttaccaacttgtcccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAGAAAAACTTACTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGTTCTGGc                                                                                                                                                         >  2:364045‑M2/150‑299 (MQ=255)

                                                                                                                           atcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGa                                                                                                                                                       >  1:476892‑M2/149‑300 (MQ=255)

                                                                                                                           atcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGa                                                                                                                                                       >  2:151700‑M2/149‑300 (MQ=255)

                                                                                                                           atcccacactgctgaanttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGNGCGGTAGACCTCTAAATCNTGCACAGGCTCTGGCGa                                                                                                                                                       >  1:668458‑M2/149‑300 (MQ=255)

                                                                                                                            tcccacactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGaa                                                                                                                                                      >  1:426295‑M2/148‑300 (MQ=255)

                                                                                                                                acactgctgaatttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAg                                                                                                                                                     <  1:224903‑M2/154‑1 (MQ=255)

                                                                                                                                            ttgttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCNGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATNAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCaaa                                                                                                                                      <  2:595615‑M2/169‑1 (MQ=255)

                                                                                                                                              gttttcagtggctgcctttttcacaaaaggggaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGc                                                                                                                                    >  1:106518‑M2/130‑300 (MQ=255)

                                                                                                                                                                     gcgagatgtgtataagagacagtggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCaa                                                                                                             >  1:54635‑M2/107‑300 (MQ=255)

                                                                                                                                                                             gaaatattgctgtatggtggattcaaccagacataaccataggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGa                                                                                                     <  1:222736‑M2/202‑1 (MQ=255)

                                                                                                                                                                             gaaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgnaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGa                                                                                                     <  1:149602‑M2/202‑1 (MQ=255)

                                                                                                                                                                              aaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactgtttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACATGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                    <  1:216062‑M2/203‑1 (MQ=255)

                                                                                                                                                                              aaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactgtttggtgaccaactggtaccagaacatgctggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTGAAACAACTTTCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                    <  2:774076‑M2/203‑1 (MQ=255)

                                                                                                                                                                              aaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGa                                                                                                     <  1:400306‑M2/202‑1 (MQ=255)

                                                                                                                                                                              aaatattgctgtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAc                                                                                                    <  1:101662‑M2/203‑1 (MQ=255)

                                                                                                                                                                                        gtatggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAaga                                                                                           >  1:654508‑M2/88‑299 (MQ=255)

                                                                                                                                                                                          atggtggattcaaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGGAGTCAAAGCTAAGTTCGGACTGCAAGGATCAAGTGTATGACGGGCAAaga                                                                                           >  2:199502‑M2/86‑297 (MQ=255)

                                                                                                                                                                                                    caaccagacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCAt                                                                              >  1:555097‑M2/76‑300 (MQ=255)

                                                                                                                                                                                                          gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  2:19444‑M2/70‑299 (MQ=255)

                                                                                                                                                                                                          gacataaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTAATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCt                                                                         >  2:336757‑M2/70‑299 (MQ=255)

                                                                                                                                                                                                              taaccagaggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGa                                                                    >  2:470290‑M2/66‑300 (MQ=255)

                                                                                                                                                                                                                      ggcatatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCataa                                                             >  1:765872‑M2/58‑299 (MQ=255)

                                                                                                                                                                                                                         atatcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGc                                                         <  1:86789‑M2/246‑1 (MQ=255)

                                                                                                                                                                                                                            tcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGNAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAAAGCTTc                                                      <  1:564959‑M2/249‑1 (MQ=255)

                                                                                                                                                                                                                            tcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTc                                                      >  1:259995‑M2/52‑300 (MQ=255)

                                                                                                                                                                                                                            tcccattgcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTc                                                      >  2:547550‑M2/52‑300 (MQ=255)

                                                                                                                                                                                                                            tcccattgcttaccggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCtt                                                       >  1:259942‑M2/52‑299 (MQ=255)

                                                                                                                                                                                                                                   gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTgtgcac                                               <  2:645960‑M2/256‑3 (MQ=255)

                                                                                                                                                                                                                                   gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTgtgcac                                               >  1:237045‑M2/45‑298 (MQ=255)

                                                                                                                                                                                                                                   gcttactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGAATGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTgtgcac                                               >  2:685754‑M2/45‑298 (MQ=255)

                                                                                                                                                                                                                                      tactgtttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTtctgtgcacagg                                            <  1:55683‑M2/259‑6 (MQ=255)

                                                                                                                                                                                                                                      tactggttggttaccaacttgtaccagaacatgggggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTtctgtgcacagg                                            <  1:232902‑M2/259‑6 (MQ=255)

                                                                                                                                                                                                                                      tactggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTtctgtgcacagg                                            <  1:54776‑M2/259‑6 (MQ=255)

                                                                                                                                                                                                                                      tactggttggttaccaactggtaccagaacatgggggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTtctgtgcacagg                                            <  1:232888‑M2/259‑6 (MQ=255)

                                                                                                                                                                                                                                      tactGGTTGGCTACCAACCTATACCAGAACATGCGTACCAATGCGCTGACTGACGCGGAATTACGGCACAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTTGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTTAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTgcacagg                                            <  2:364348/300‑6 (MQ=255)

                                                                                                                                                                                                                                         tggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTTAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGcttctgtgcacaggac                                          <  1:99734‑M2/261‑8 (MQ=255)

                                                                                                                                                                                                                                         tggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGcttctgtgcacaggac                                          <  1:99952‑M2/261‑8 (MQ=255)

                                                                                                                                                                                                                                         tggttggttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGcttctgtgcacaggac                                          <  1:98481‑M2/261‑8 (MQ=255)

                                                                                                                                                                                                                                               gttaccaacttgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGcc                                    >  1:205001‑M2/33‑299 (MQ=255)

                                                                                                                                                                                                                                               gttaccaaattgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGAACTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTNGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGc                                     >  2:624989‑M2/33‑298 (MQ=255)

                                                                                                                                                                                                                                                         tgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGTATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAgg                         <  2:559608‑M2/278‑1 (MQ=255)

                                                                                                                                                                                                                                                         tgtaccagaacatgcgggccaaTGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAAC‑TGTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAgg                         <  1:60856‑M2/278‑1 (MQ=255)

                                                                                                                                                                                                                                                                      gctggCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGATGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAAcc            <  2:319460/297‑1 (MQ=255)

                                                                                                                                                                                                                                                                      gCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTT‑TCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGANCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGNATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGTGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAAcc            <  2:595086/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                      gCGGGCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAANCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAAcc            <  1:151700/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                         ggCCAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGc         >  1:391587/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                           ccAATGCGCTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCAt       >  2:48960/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                gcgcTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTNCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTATGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATc  >  2:566807/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                gcgcTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATc  >  1:90308/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                gcgcTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCCGTGCACAGGCCGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATc  >  2:164976/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                gcgcTGACTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGAAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGAAGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATc  >  2:222905/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                  gctgaCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACT‑GTCCTGTATGACCGCGCGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGATCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGCACAGGACGTTGCCGCGGTAACAGGTGCAACCGTAACCAGCATAAATc  <  2:54635/294‑1 (MQ=255)

ACGGGATCACCACGATGGAACAGGTTAACGCAGGAATGCGCGTAGCCCGTCGGCAGAATCGACCATTTCTGCCATCACCCGGGCAGTTTGTTGCATGGTGCCGGGAAGAAGCATCCGTTACCGCCGGACTGCCAAACGTCAGCGAGCTGGTTGATATGGTTTACGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATCCGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCAATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCC‑ATATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAACCAGCATAAATC  >  NC_012971/762590‑763161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`