Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012971	642,526	A→T	K5* (AAA→TAA)	ECD_RS25105 →	IS3‑like element IS150 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012971	642,526	0	A	T	100.0%	245.1 / NA	73	K5* (AAA→TAA)	ECD_RS25105	IS3‑like element IS150 family transposase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (36/37);  total (36/37)

GGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATC  >  NC_012971/642242‑642763                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               ggTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGANACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGaaa                                                                                                                                                                                                                                 >  2:619307/1‑300 (MQ=255)      ccaccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAgcgc                                                                                                                                                                                                                            >  1:73458/1‑300 (MQ=255)      ccaccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTACGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAgcgc                                                                                                                                                                                                                            >  1:493917/1‑300 (MQ=255)         ccaTCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTACGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCACGAAACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCtttt                                                                                                                                                                                                                                     >  2:336649/1‑288 (MQ=255)           atcatGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTACGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCATAATATGTCAAAGCCATACTACCCTTTTGAAAAGCGC‑ATTGa                                                                                                                                                                                                                      >  2:459009/1‑300 (MQ=255)              atGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCNGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGt                                                                                                                                                                                                                   <  1:564018/300‑1 (MQ=255)                   cATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGa                                                                                                                                                                                                              >  1:626795/1‑300 (MQ=255)                                   aGTTCCACCATCACGTCCATTACTTCGTTCATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTTAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAAc                                                                                                                                                                                              <  2:271635/300‑1 (MQ=255)                                   aGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTTACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAAc                                                                                                                                                                                              <  2:271844/300‑1 (MQ=255)                                   aGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGATCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTTAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAAc                                                                                                                                                                                              <  2:201685/300‑1 (MQ=255)                                   aGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAAc                                                                                                                                                                                              <  1:27118/300‑1 (MQ=255)                                    gTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACGCGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGCTGGACGATGAAATGGAGGAGCCCCCAATATTTCAAAGCCATAATCGCCCTTTGAAAAGCGCGCCTGAAGTCGTGGATCACTCCTTCACaa                                                                                                                                                                                               >  2:265372/1‑299 (MQ=255)                                                cGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTAc                                                                                                                                                                                 >  1:55570/1‑300 (MQ=255)                                                 gTCCAGTCCTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGTGAGCGATTGCCACACGCTACTGCTGACCGCCGGAAAGCTGCGCCGGGAACTTATTGGCGGGGGCAGAAAACCCGACACGCTCAAGCAGCTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCACCTAATATGTCAAAGCCCTAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGCATCACTCCTTAACAACTGATGATGGTTACa                                                                                                                                                                                >  2:59819/1‑300 (MQ=25)                                                 gTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACa                                                                                                                                                                                >  2:357276/1‑300 (MQ=255)                                                    cAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCTTGAATCACTACTTCAAAACTGATGATGGTTACAGGa                                                                                                                                                                             >  2:707714/1‑300 (MQ=255)                                                    cAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACAAAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCTTGAGCAGAAAGCCCGACACGCTCAAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATGCCCTTTTGAAAAGCGC‑ATTGAAGTCGTGAATCACTAATTCAAAACTGATGACGGTTaaa                                                                                                                                                                                >  2:708255/1‑295 (MQ=37)                                                                       tcCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTgg                                                                                                                                                          <  1:199493/300‑1 (MQ=255)                                                                            aTCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGACAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTcc                                                                                                                                                     >  2:357386/1‑300 (MQ=255)                                                                            aTCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGAAACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCAATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTcc                                                                                                                                                     >  2:356422/1‑300 (MQ=255)                                                                                aGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTcccc                                                                                                                                                   <  2:655033/298‑1 (MQ=255)                                                                                 gcgcCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGa                                                                                                                                                <  1:629074/300‑1 (MQ=255)                                                                                      gATGTCGGTTCGTCAAACAGCATCGCAATCGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATATCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCACCGAACCCa                                                                                                                                           >  2:690431/1‑300 (MQ=255)                                                                                          tCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACACCGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGTAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTc                                                                                                                                       <  2:39841/300‑1 (MQ=255)                                                                                           cGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCa                                                                                                                                      >  1:184598/1‑300 (MQ=255)                                                                                               tCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGNAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGAc                                                                                                                                  <  2:568354/300‑1 (MQ=255)                                                                                               tCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGAc                                                                                                                                  <  2:162258/300‑1 (MQ=255)                                                                                               tCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAAGATCACCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGAc                                                                                                                                  <  2:162226/300‑1 (MQ=255)                                                                                               tCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTAATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGAc                                                                                                                                  <  1:634766/300‑1 (MQ=255)                                                                                               tCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGNACTGCACCCATTTTGTTGGACGATTAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGAc                                                                                                                                  <  2:241994/300‑1 (MQ=255)                                                                                                    agacggCATCGCAATAGGATCCATACACAACACTCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGTTCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAGAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACTTTTTGGTGTCCCCCGAACCCAGGTCAGGACATggg                                                                                                                             <  2:321657/295‑1 (MQ=21)                                                                                                    aaaCAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTAGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATggg                                                                                                                             <  1:549542/300‑1 (MQ=255)                                                                                                    aaaCAGCATCGCAATAGGATCCAGACACAACACGCGAGCGATTGCCACCCGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATTTCAAAGCCATAATACCCTTTTTAAAAGCGC‑CTTGAAGTCTTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATggg                                                                                                                             <  2:318338/300‑1 (MQ=255)                                                                                                        aGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTg                                                                                                                          <  1:105494/299‑1 (MQ=255)                                                                                                           aTCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGccc                                                                                                                       <  1:637656/299‑1 (MQ=255)                                                                                                                       tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACa                                                                                                          >  1:113230/1‑300 (MQ=255)                                                                                                                       tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACa                                                                                                          >  2:639683/1‑300 (MQ=255)                                                                                                                       tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCAGAACCCAGGTCAGGACATGGGTTGCCCTCTATGCAAAACa                                                                                                          >  2:690656/1‑300 (MQ=255)                                                                                                                       tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTAGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACa                                                                                                          >  2:288727/1‑300 (MQ=255)                                                                                                                       tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCAAAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACa                                                                                                          >  1:44414/1‑300 (MQ=255)                                                                                                                       tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCAAAACTGATGATGGTTACAGGATCATCTAGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACa                                                                                                          >  2:497451/1‑300 (MQ=255)                                                                                                                       tCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAATCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACa                                                                                                          >  2:39805/1‑300 (MQ=255)                                                                                                                         cATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAGAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTAGGCACGTTTTGGTGTCCACCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACAt                                                                                                         >  2:3449/1‑299 (MQ=255)                                                                                                                                               gCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTCCTATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTCTGAAAAACATGGAGAAAAAGGTTTAATTCCCaa                                                                                  >  1:461632/1‑300 (MQ=255)                                                                                                                                               gCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCaa                                                                                  >  1:461391/1‑300 (MQ=255)                                                                                                                                                       ctgctgctgACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTaaa                                                                           >  1:599700/1‑299 (MQ=255)                                                                                                                                                        tgctgctgACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAgg                                                                         <  1:431878/300‑1 (MQ=255)                                                                                                                                                         gctgctgACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCCGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGc                                                                        >  2:385407/1‑300 (MQ=255)                                                                                                                                                         gctgctgACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTaaa                                                                           >  2:538351/1‑297 (MQ=255)                                                                                                                                                                aCCGCCTGAAAGCTACGCCTGAAACTTATTGGCGCGAGCAGAAAGCCCGACACGCTCCGGCAGTTTTGTACTACACCCATTTTGTTGTACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAATTCGTGAACCACTACTTcacacc                                                                                                                                                                                              <  2:371342/175‑3 (MQ=18)                                                                                                                                                                      ggAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAAGGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGTTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATc                                                           <  2:423510/300‑1 (MQ=255)                                                                                                                                                                           gCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGTAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCagag                                                      <  2:361699/300‑1 (MQ=255)                                                                                                                                                                             tGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGt                                                     <  1:800750/299‑1 (MQ=255)                                                                                                                                                                                         ttATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAgg                                         <  1:385407/299‑1 (MQ=255)                                                                                                                                                                                         ttATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCGTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAgg                                         <  1:690431/299‑1 (MQ=255)                                                                                                                                                                                          tATTGGCGTGAGCAGAAAGCCCTACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGTTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGt                                        <  2:498729/299‑1 (MQ=255)                                                                                                                                                                                                  tGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGaaa                                 <  1:288727/298‑1 (MQ=255)                                                                                                                                                                                                   gcgCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAACGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTACAAAACCTAAAGGCGTTCGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCt                              >  2:215213/3‑300 (MQ=255)                                                                                                                                                                                                            gCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTTATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGANAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGa                      >  1:582144/1‑299 (MQ=255)                                                                                                                                                                                                            gCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGGAAAAGGTTTAATTCCAAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGa                      >  2:16169/1‑299 (MQ=255)                                                                                                                                                                                                                gACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGagc                    <  2:723344/297‑1 (MQ=25)                                                                                                                                                                                                                 acacGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCACACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAgcagca                >  1:289547/1‑300 (MQ=25)                                                                                                                                                                                                                   acGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGcac               <  1:639683/299‑1 (MQ=21)                                                                                                                                                                                                                   acGCTCCAGCAGTTTGGGACTGCACCCATTTTGTTGGACGATGAAAGGGAATGGCCCCTAATATGTCAAAGCCATAATAACCTTTTGAAAAGCGC‑CTTGAATTCAGGAATCACTAATTCACAACTGATGATGGGGACAGGATCATCTCGGCACGGTTTGGTGTCCCCCGAACTCAGGTCAGGACATGGGTGGCCCTCTCGGAAAAACATGGATAAAAAGGTTTGATTCCTAAGCCGAAAGGCGTTAGGGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCGGTGATCGAGCAGcac               <  1:639693/299‑1 (MQ=11)                                                                                                                                                                                                                      cTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTAGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGTTGATCCAGAGTTGCGTATTCAGGTCGTGAAAGCTGTGCTCGAGCAGCACATGt           >  2:303809/1‑300 (MQ=255)                                                                                                                                                                                                                       tCCAGCATTTTTGTATTGCACCCCTTTTGTTGTACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTc          <  2:427728/300‑1 (MQ=12)                                                                                                                                                                                                                       tCCAGCATTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAGAGCCCCTAATATGTCAAAGCCATAATACCCTTTGGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAGACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTc          <  2:427612/300‑1 (MQ=12)                                                                                                                                                                                                                       tCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTc          <  2:73458/300‑1 (MQ=18)                                                                                                                                                                                                                          agtagTTTTGTACTGCACCCATTTTGTTGTACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCt       <  2:661821/297‑1 (MQ=14)                                                                                                                                                                                                                          agcagTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCt       <  2:661832/300‑1 (MQ=17)                                                                                                                                                                                                                              gTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAAt   >  1:484446/1‑300 (MQ=14)                                                                                                                                                                                                                              gTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCACTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCAAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAAt   >  1:484203/1‑300 (MQ=14)                                                                                                                                                                                                                               ttttGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCACTAATATGGCAAAGCCATAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATc  <  2:403027/300‑1 (MQ=11)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               GGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGAAACTTATTGGCGTGAGCAGAAAGCCCGACACGCTCCAGCAGTTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGC‑CTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATC  >  NC_012971/642242‑642763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑