Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_012971	3,997,577	A→G	F459F (TTT→TTC)	frvB ←	PTS fructose‑like transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_012971	3,997,577	0	A	G	100.0%	323.2 / NA	90	F459F (TTT→TTC)	frvB	PTS fructose‑like transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/44);  total (46/44)

GAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATAAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC  >  NC_012971/3997281‑3997871                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       gAAAACCGCTCTCGTCGATGTGGGTGACCAGAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACTGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATgaat                                                                                                                                                                                                                                                                                                     <  2:754955/300‑1 (MQ=255)      ccGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATgaatgaaat                                                                                                                                                                                                                                                                                                >  1:408616/1‑300 (MQ=255)           ctcGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCa                                                                                                                                                                                                                                                                                           >  2:299074/1‑300 (MQ=255)             cgtcgCTGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATTGAGGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAATAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTAAGGACGATAATGAATGAAATGTTCAgc                                                                                                                                                                                                                                                                                         >  1:96905/1‑300 (MQ=255)               tcgATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAgcgc                                                                                                                                                                                                                                                                                       <  1:4720/300‑1 (MQ=255)                cgATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAgcgc                                                                                                                                                                                                                                                                                       >  1:254381/1‑299 (MQ=255)                cgATGTAGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTAAGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCCCAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAAACACAATCCTTTCAGGACGATAATGAATGAAGTGTTCAgcgc                                                                                                                                                                                                                                                                                       >  1:254568/1‑299 (MQ=255)                    gtgGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCATTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGc                                                                                                                                                                                                                                                                                  >  1:220007/1‑300 (MQ=255)                    gtgGGTGACCATAAAGACGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGTGCAACAAAGCTGCCCAGACCATCGAAGTTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGc                                                                                                                                                                                                                                                                                  <  2:122224/300‑1 (MQ=255)                                 aaGCCGACTTCATTCATATGTCCGACAACGGCAACTTTTGGACCGTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGGGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTGCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCAc                                                                                                                                                                                                                                                                     <  1:466352/300‑1 (MQ=255)                                 aaGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCAc                                                                                                                                                                                                                                                                     <  1:448477/300‑1 (MQ=255)                                      gACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGAt                                                                                                                                                                                                                                                                <  1:37048/300‑1 (MQ=255)                                          tCATCCCTATGTCCGCCCACGGCAACTTTTGGACCTTTATTCCCCTTAAGAGCACCGAAGCTGCCCAGACCATTGCAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGGGAACTTCCTGTTCATCGCCGATGACGGCGCTGGCTTCGCACAACCGTTGCAGTAACTCCCTGTTCATGGACAAGCTCCTGTTGCACGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTAAGGCCGATAATGAATGAAATGTTCAGCGTTAGGCCAATTGTCAGCACCAGATaa                                                                                                                                                                                                                                                              >  2:99765/1‑298 (MQ=255)                                          tCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACCAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGACGCTGCCGGCGCTGGCTTCGCCCAACTGTTGTAGTAACTAAATGTTCATGGAAAAGTTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAAAAACAATCCTTTCAGGACGATGATGAATGAAATGTTCAGCGCGAGGCCAATCGACAGCACCAGa                                                                                                                                                                                                                                                                 >  2:99758/1‑295 (MQ=255)                                          tCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATaaaa                                                                                                                                                                                                                                                            >  1:18403/1‑300 (MQ=255)                                           cATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCAGCTGACGGCGCTGGCTTCGCACAANTGTTGCAGTAACTCCATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCCGGCCGATAATGAATGACATGTTCAGCGCGCGGCAAACCGCCAGCACCAGATaaaa                                                                                                                                                                                                                                                            >  2:239527/1‑299 (MQ=255)                                           cATCCACATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGATGGCTTCGCACAANTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCCGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCAAGCATCAGATAAAAc                                                                                                                                                                                                                                                           >  2:239520/1‑300 (MQ=255)                                            aTCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAAc                                                                                                                                                                                                                                                           <  1:647674/299‑1 (MQ=255)                                              ccATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCAAGCACCAGATAAAACGaa                                                                                                                                                                                                                                                        >  2:111942/1‑300 (MQ=255)                                                            aCGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACAt                                                                                                                                                                                                                                          >  1:232968/1‑300 (MQ=255)                                                              ggCAACTTTTGGACCTTTATTCCCCTTGCGTGCAACAAAGCTGCCCAGACCATCGAATGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGAGAAAACGAACCCACCGGCGACATc                                                                                                                                                                                                                                         <  2:313416/299‑1 (MQ=255)                                                                           ccTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCAAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGc                                                                                                                                                                                                                             >  1:372128/1‑298 (MQ=255)                                                                             tttATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGt                                                                                                                                                                                                                         >  1:397212/1‑300 (MQ=255)                                                                                        tACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATAccc                                                                                                                                                                                                              <  1:502218/300‑1 (MQ=255)                                                                                                  aaaGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGTGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGTGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCCTGGCCAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATGATGAATGAAATGTTCAGCGCGAGGCCGATAGCCCGCCCCCGATAAAACGCACCCACCGGTGACATCAGGCCGATCCGCGGGTCAAAGATAcac                                                                                                                                                                                                              >  2:107529/1‑288 (MQ=255)                                                                                                  aaaGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGt                                                                                                                                                                                                     >  2:525020/1‑299 (MQ=255)                                                                                                  aaaGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTg                                                                                                                                                                                                    <  1:554157/300‑1 (MQ=255)                                                                                                        gCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGAc                                                                                                                                                                                              >  1:615975/1‑300 (MQ=255)                                                                                                             gACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGTGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGttt                                                                                                                                                                                          >  1:332978/1‑299 (MQ=255)                                                                                                             gACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCGAAGATACCCAAACCAGGTGCCAGACGttt                                                                                                                                                                                          >  2:188902/1‑299 (MQ=255)                                                                                                             gACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGttt                                                                                                                                                                                          >  2:643137/1‑299 (MQ=255)                                                                                                             gACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGttt                                                                                                                                                                                          >  1:637423/1‑299 (MQ=255)                                                                                                                 aTCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGAt                                                                                                                                                                                       <  1:375518/298‑1 (MQ=255)                                                                                                                        gTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCAAGACGTTTGATTCCGAAAGc                                                                                                                                                                              >  1:398998/1‑300 (MQ=255)                                                                                                                          gATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGa                                                                                                                                                                            >  1:763973/1‑300 (MQ=255)                                                                                                                                attcaCGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTNGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCCAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCAcc                                                                                                                                                                       >  2:576537/1‑299 (MQ=255)                                                                                                                                    aCGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACNGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCAc                                                                                                                                                                  <  1:584271/300‑1 (MQ=255)                                                                                                                                           gTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCCCCAGCACGCCGGTa                                                                                                                                                           >  2:455284/1‑300 (MQ=255)                                                                                                                                           gTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTa                                                                                                                                                           >  1:54651/1‑300 (MQ=255)                                                                                                                                           gTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTa                                                                                                                                                           >  2:455367/1‑300 (MQ=255)                                                                                                                                           gTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCAACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTa                                                                                                                                                           >  2:370307/1‑300 (MQ=255)                                                                                                                                           gTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAACNCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACNACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCAAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTa                                                                                                                                                           >  2:610853/1‑300 (MQ=255)                                                                                                                                                        tATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCa                                                                                                                                              >  1:755823/1‑300 (MQ=255)                                                                                                                                                              aaTGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTAt                                                                                                                                        <  1:608738/300‑1 (MQ=255)                                                                                                                                                                 gTCGCGAACTTCCTGTTNATCGCCGCTGACGGCGCTNGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCNCCCGCCAGCGTATTgg                                                                                                                                     <  1:664638/300‑1 (MQ=255)                                                                                                                                                                  tCGCGAACTTCCTGTTCATCGCCGCGGACGGCACTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCTGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCTAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTgg                                                                                                                                     <  2:403133/299‑1 (MQ=255)                                                                                                                                                                    gcgAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGATGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCg                                                                                                                                   <  2:643951/299‑1 (MQ=255)                                                                                                                                                                    gcgAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCg                                                                                                                                   <  2:643998/299‑1 (MQ=255)                                                                                                                                                                             cTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAatcatc                                                                                                                          >  2:311441/1‑299 (MQ=255)                                                                                                                                                                                             aCGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTT‑AGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCaga                                                                                                        <  1:111076/300‑1 (MQ=255)                                                                                                                                                                                                gcgcTTGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCagag                                                                                                       <  2:145558/299‑1 (MQ=255)                                                                                                                                                                                                  gcTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCg                                                                                                     <  1:129449/299‑1 (MQ=255)                                                                                                                                                                                                        ttCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGTTAATGCCGCCCGCCAGCGTATTGGCGTTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGa                                                                                               <  2:137017/299‑1 (MQ=255)                                                                                                                                                                                                        ttCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGa                                                                                               <  1:136027/299‑1 (MQ=255)                                                                                                                                                                                                         tCGCACAACTGGTGCAGTAACTCAATGTTCATGGACAAGCTCCTTTTGCGCGGCTGTCGCTTGGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATTAAATGTTCAGCGCTAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCTGCGACATCAGGCAGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGTCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAAt                                                                                             <  2:186189/300‑1 (MQ=255)                                                                                                                                                                                                             acaACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGcc                                                                                         <  1:179821/300‑1 (MQ=255)                                                                                                                                                                                                                       cAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCcggttcggtt                                                                                >  1:195271/1‑299 (MQ=255)                                                                                                                                                                                                                       cAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCCGCCAGAGCGTAAGGAATAGCcggttcggtt                                                                                >  1:195659/1‑299 (MQ=255)                                                                                                                                                                                                                       cAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCAGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCcggttcggtt                                                                                >  1:195856/1‑299 (MQ=255)                                                                                                                                                                                                                        aGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTg                                                                               <  2:678013/299‑1 (MQ=255)                                                                                                                                                                                                                           aaCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAATGAATAGCCGGTTCGGTTGctgt                                                                           <  1:107529/300‑4 (MQ=255)                                                                                                                                                                                                                           aaCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGctgt                                                                           <  1:525020/300‑4 (MQ=255)                                                                                                                                                                                                                           aaCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGAc                                                                           <  1:486514/300‑1 (MQ=255)                                                                                                                                                                                                                            aCTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACt                                                                          >  2:756662/1‑300 (MQ=255)                                                                                                                                                                                                                             cTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACt                                                                          >  1:492747/1‑299 (MQ=255)                                                                                                                                                                                                                                   gTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCAc                                                                   <  2:725137/300‑1 (MQ=255)                                                                                                                                                                                                                                   gTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCAc                                                                   <  1:536227/300‑1 (MQ=255)                                                                                                                                                                                                                                           aCAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAGTGAATGAAATGTTCAGCGCTAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCTGCAGCGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACcac                                                            <  2:15752/299‑1 (MQ=255)                                                                                                                                                                                                                                             aaGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAAt                                                         <  2:637423/300‑1 (MQ=255)                                                                                                                                                                                                                                             aaGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACAATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAACGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAAt                                                         <  2:91425/300‑1 (MQ=255)                                                                                                                                                                                                                                              aGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAAt                                                         >  1:637487/1‑299 (MQ=255)                                                                                                                                                                                                                                              aGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCAGGTTCGGTTGCGACTGTGGCACCGACCACAAt                                                         >  1:71727/1‑299 (MQ=255)                                                                                                                                                                                                                                              aGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGCCAAAGATACCCAAACCAGGTGCCAGCCGTTTGATTCCGAAAGCGATCACCAGCCCGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTACGACTGTGGCACCGACCAAAAt                                                         >  1:637478/1‑299 (MQ=255)                                                                                                                                                                                                                                               gCTCCTTTTGCGCGGCTTTCGCTTTGCGGCGTGACCACAATCCTTCCAGTACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCTCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACTTTTGATTCCGAAAGCGACCACCAGCACGCCGGTAGTGCCTCCCGCCAGCGTATTGGCGTTAATCATCGGCAACGGAGCTGCCAGAGCGTAATGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATgg                                                       <  2:96905/300‑1 (MQ=255)                                                                                                                                                                                                                                               gCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATgg                                                       <  2:774247/300‑1 (MQ=255)                                                                                                                                                                                                                                                ctcatGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTNTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCAGCCCGACAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCAGGTTCGGTTGCGACTGTGGCACCGACCACaa                                                          >  2:236406/5‑296 (MQ=255)                                                                                                                                                                                                                                                cTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGGCGTTTGATTCCGAAAGCAATCACCAGCACGCCGGTAATGCCGCCCGCCGGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCAGGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACCATgga                                                      >  2:392373/1‑299 (MQ=255)                                                                                                                                                                                                                                                cTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGc                                                      >  1:682137/1‑300 (MQ=255)                                                                                                                                                                                                                                                cTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCAGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCAGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGc                                                      >  1:682038/1‑300 (MQ=255)                                                                                                                                                                                                                                                    ttttGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGTACGATAATGAAGGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCATGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGATGTTGGATTCCGAAAGCGATCACCAGCACGCCGTTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAAGAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCac                                                    <  2:146540/296‑1 (MQ=255)                                                                                                                                                                                                                                                    tGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGTACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAATCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCac                                                    <  2:288981/298‑1 (MQ=255)                                                                                                                                                                                                                                                    tGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCac                                                    <  1:111942/298‑1 (MQ=255)                                                                                                                                                                                                                                                     gTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGATGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCAATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCactac                                                 <  2:54651/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCNAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGTTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCTGTTCGGTTGCGACTGTGGCACCGACCACAATGGCactac                                                 <  2:246919/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCactac                                                 <  1:299074/300‑1 (MQ=255)                                                                                                                                                                                                                                                          gcgGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCa                                            <  1:110673/300‑1 (MQ=255)                                                                                                                                                                                                                                                            ggCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGc                                          <  2:220007/300‑1 (MQ=255)                                                                                                                                                                                                                                                             gCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGcc                                         >  2:730371/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           gcgTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGNCGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTc                                 >  2:570155/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                   acaATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGTTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc                    <  1:99765/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   acaATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc                    <  1:99758/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                          tttCAGGACGATAATGNATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACNGGCAGCCTCTTTTTCCTGNCGGGTAAACAGttt               >  1:660182/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                                    ataatgAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGNTACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCATCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAc  <  2:615975/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       GAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGACCTTTATTCCCCTTACGGGCAACAAAGCTGCCCAGACCATCGAAGGTGATTTCATTCACGCAAGGTTCCAGCGTGTTTATCAGAATGTCGCGAACTTCCTGTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATAAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC  >  NC_012971/3997281‑3997871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑