Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,011,802	C→T	P243S (CCG→TCG)	bioP →	biotin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,011,802	0	C	T	100.0%	171.6 / NA	53	P243S (CCG→TCG)	bioP	biotin transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/41);  total (12/41)

GCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGC  >  NC_000913/4011507‑4012079                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                      gCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTTGCAGTGATTGCATGGTTCTTGCTTGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGc                                                                                                                                                                                                                                                                                   <  1:257739/300‑1 (MQ=255) gCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGc                                                                                                                                                                                                                                                                                   <  1:256971/300‑1 (MQ=255)       ctcCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGgct                                                                                                                                                                                                                                                                             >  1:629844/1‑300 (MQ=255)            atatCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGgctgct                                                                                                                                                                                                                                                                          >  1:726694/1‑298 (MQ=255)                   ttttGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCNTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCt                                                                                                                                                                                                                                                                 <  2:570402/300‑1 (MQ=255)                   ttttGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTTGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAAcc                                                                                                                                                                                                                                                                  <  1:290500/299‑1 (MQ=255)                           ttGGCATGGTGGGGTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTTGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGCTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCt                                                                                                                                                                                                                                                         <  2:513571/300‑1 (MQ=255)                                aTGGTGGGTTACAAACGNCTGATGGAAACTCGCCCGNTGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAANCCTGGCTATCTGGCAc                                                                                                                                                                                                                                                    <  1:610837/300‑1 (MQ=255)                                      ggTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACa                                                                                                                                                                                                                                               >  1:71035/1‑299 (MQ=255)                                            aaaCGCCTGATGGAAACTCGCCCGATGCAACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATTTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCAc                                                                                                                                                                                                                                        <  2:24333/300‑1 (MQ=255)                                                   tgagggAAACTCGCCCGATGCCACAACATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTTCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCTGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTTGCACCAACAGCCGCACTGGcc                                                                                                                                                                                                                                   <  2:12922/294‑1 (MQ=255)                                                           aCTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGTTGGCAGTGATTGCATGTTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATTGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGNTACTTTATGTGGAACTACGGCGCGACGCAGGTNGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTttatt                                                                                                                                                                                                                         <  2:560377/300‑1 (MQ=255)                                                             tCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTNCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTAc                                                                                                                                                                                                                       <  1:157841/300‑1 (MQ=255)                                                                   taTGCCACAGCATAACGCCTTTTCTTGGTTCTATCTTGGCGCGTTTCTGGTGGCATTGATTTCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCTGAACGCTGGGAATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCCGGCACCAACAGCCGCACTGGCCAACGTTTATTACAgg                                                                                                                                                                                                                    <  2:416645/296‑1 (MQ=255)                                                                   gattctacaGCATAACTCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGgcg                                                                                                                                                                                                                  <  2:157239/293‑1 (MQ=255)                                                                     tGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTAAGGCGCGACGAAGGTGGACGCAGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATTTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGgcgcgc                                                                                                                                                                                                               >  2:298391/1‑300 (MQ=255)                                                                       ccACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCAGCACTGGCCAACGGTTATTAAAGGCGCGCt                                                                                                                                                                                                              >  2:288305/1‑299 (MQ=255)                                                                                   gCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGcc                                                                                                                                                                                                 <  1:565977/300‑1 (MQ=255)                                                                                      tttGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTc                                                                                                                                                                                               <  1:356831/299‑1 (MQ=255)                                                                                       tttcGTGGTTCTATCTTGGCGCGTTTCTGGGGGCAGTGATTGCATGGTCCTTGCTTGGAAATGCGCAGAAAATGCCACAAACCACGCTGCAATGGTGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCAc                                                                                                                                                                                             <  2:328431/297‑1 (MQ=255)                                                                                       ttGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCAc                                                                                                                                                                                             <  1:126861/300‑1 (MQ=255)                                                                                        tGCGTGGTTCTATCTTTGCGCCTTTCGTGTGGCAGGGATTGCAGGGTTCTTGCTGGTAAATGCGCAGAAAATGCCGCAAACCACACTGCAATGGGGCATTCTGGTTTTTCTTGGCTTGGTGGCTTCCGGGATTTGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACt                                                                                                                                                                                            <  2:352388/300‑1 (MQ=255)                                                                                         gCGTGGTTCTATCTTGGCGCGTTTCTGTTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGTGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACtg                                                                                                                                                                                           <  2:707690/300‑1 (MQ=255)                                                                                             ggTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTggg                                                                                                                                                                                       <  1:690708/300‑1 (MQ=255)                                                                                              gTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGTAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGt                                                                                                                                                                                      <  1:291153/300‑1 (MQ=255)                                                                                              gTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGt                                                                                                                                                                                      >  2:150741/1‑300 (MQ=255)                                                                                              gTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGt                                                                                                                                                                                      >  1:51/1‑300 (MQ=255)                                                                                              gTTCTATCTTGGCGCGTTTATGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGt                                                                                                                                                                                      >  2:167238/1‑300 (MQ=255)                                                                                               ttCTATCTTTGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGTGCATTCTGGGGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTg                                                                                                                                                                                     <  1:421030/300‑1 (MQ=255)                                                                                                  tATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCTGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCAt                                                                                                                                                                                  <  2:613586/300‑1 (MQ=255)                                                                                                   aTCTTTGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTTGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCATCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATc                                                                                                                                                                                 <  2:415365/300‑1 (MQ=255)                                                                                                    tCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCg                                                                                                                                                                                <  1:276723/300‑1 (MQ=255)                                                                                                           gcgTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTg                                                                                                                                                                          <  2:605236/299‑1 (MQ=255)                                                                                                             ttttCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGt                                                                                                                                                                       <  2:310450/299‑1 (MQ=255)                                                                                                              tttCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTc                                                                                                                                                                      >  1:676633/1‑300 (MQ=255)                                                                                                                                         ttGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGTGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGNTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAga                                                                                                                                            <  2:239593/299‑1 (MQ=255)                                                                                                                                               ggAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGc                                                                                                                                     <  2:612540/300‑1 (MQ=255)                                                                                                                                                               tGCCGCAAACCACGCTGCAATGGTGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTgcgcgc                                                                                                                     <  1:411239/300‑1 (MQ=255)                                                                                                                                                                              tGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGTGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGc                                                                                                      <  1:178104/300‑1 (MQ=255)                                                                                                                                                                                                  tttCTTGGCGTGGTGGCTTCCTGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTTGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACgc                                                                                   <  2:112113/299‑1 (MQ=255)                                                                                                                                                                                                                  cttacGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCAc                                                                  >  2:490454/5‑300 (MQ=255)                                                                                                                                                                                                                  cTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGNCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTACGTAACTGGCTGACGCTGCTCGCCATCGCGCAc                                                                  >  1:154022/1‑300 (MQ=255)                                                                                                                                                                                                                         gATTGGCCACTTTATTTGGAACTACGGCGCAACGCAGGTATACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTTGCCTCACTGTGCGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGTCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCAc                                                                  <  2:314601/293‑1 (MQ=255)                                                                                                                                                                                                                              gCTACTTTATTTGGAACTACTGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTTTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAg                                                      <  2:56768/300‑1 (MQ=255)                                                                                                                                                                                                                                tACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTc                                                    <  2:726694/300‑1 (MQ=255)                                                                                                                                                                                                                                 aCTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCg                                                   <  2:628699/300‑1 (MQ=255)                                                                                                                                                                                                                                         tgGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCcaca                                           >  1:111504/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gCAGGTGGAAGCTTGAACGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCACTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGTAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGt                           <  2:133555/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         aaCGCTGTGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTTGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTgg                      <  2:285346/289‑1 (MQ=255)                                                                                                                                                                                                                                                                          aCGCTGGGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGAc          <  1:298910/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            gcggggCATTATGAATAACATGCACGTTTCGGCAGGGCGGCTGGTAAACCTTGCTATCTGGCACCAATAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTCGGTGCATCGTAAGTGGTTCGCTCCGCGCTCTCCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGAcc         <  2:215414/296‑1 (MQ=255)                                                                                                                                                                                                                                                                            gCTGTGCATTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGAc          <  2:176910/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aTTATGAATAATATGCACGTTTCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACATGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGgcgc  <  2:3048/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                      GCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGGTCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCACGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGC  >  NC_000913/4011507‑4012079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑