Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,869,637	C→A	L87L (CTC→CTA)	cbrA →	colicin M resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,869,637	0	C	A	100.0%	329.5 / NA	90	L87L (CTC→CTA)	cbrA	colicin M resistance protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (43/47);  total (43/47)

GTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCCAGCAGTGGTTCGCGGAGAAACAT  >  NC_000913/3869343‑3869931                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       gTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGCCGTCGCCGCATCGCTAAcgc                                                                                                                                                                                                                                                                                                    >  1:431649/1‑299 (MQ=255) gTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAAcgcg                                                                                                                                                                                                                                                                                                   <  1:119202/300‑1 (MQ=255)      ttGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGTCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACt                                                                                                                                                                                                                                                                                              <  2:197497/300‑1 (MQ=255)       tGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGTCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTa                                                                                                                                                                                                                                                                                             <  2:509710/300‑1 (MQ=255)        gCCATCTTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTAc                                                                                                                                                                                                                                                                                            <  1:119050/300‑1 (MQ=255)        gCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACTTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTAc                                                                                                                                                                                                                                                                                            <  2:393358/300‑1 (MQ=255)        gCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTAc                                                                                                                                                                                                                                                                                            <  1:119251/300‑1 (MQ=255)               tgatgaATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTANTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGaa                                                                                                                                                                                                                                                                                     >  2:529334/1‑300 (MQ=255)               tgatgaATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGaa                                                                                                                                                                                                                                                                                     >  1:409321/1‑300 (MQ=255)                 atgaATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGc                                                                                                                                                                                                                                                                                   >  1:559043/1‑300 (MQ=255)                        tgagtgaAAAGATGGAANATTTCGACGTGGCGATTANTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTANCAGCGAAGCTATATCa                                                                                                                                                                                                                                                                            <  1:620255/300‑1 (MQ=255)                        tgagtgaAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCa                                                                                                                                                                                                                                                                            <  1:566060/300‑1 (MQ=255)                                 aGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATc                                                                                                                                                                                                                                                                   <  1:648283/300‑1 (MQ=255)                                                           attGGCCTCGGCCCGGNAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATNAATATTAATCGCCATGCTNTCGACTTGTGGATGaaa                                                                                                                                                                                                                                         >  1:615638/1‑300 (MQ=255)                                                                          gCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGcc                                                                                                                                                                                                                          <  1:716850/300‑1 (MQ=255)                                                                              gATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATTGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCACGCCAGc                                                                                                                                                                                                                       >  2:367445/1‑299 (MQ=255)                                                                               aTCGGCGTTGGCACGAAAGTGAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGt                                                                                                                                                                                                                     <  2:45909/300‑1 (MQ=255)                                                                                   gCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCNCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGAATTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGa                                                                                                                                                                                                                  >  2:563290/1‑299 (MQ=255)                                                                                           aCGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCa                                                                                                                                                                                                         <  1:595561/300‑1 (MQ=255)                                                                                            gtccttaccgCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTCTCaa                                                                                                                                                                                                        >  2:501038/10‑299 (MQ=255)                                                                                              aaaGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGa                                                                                                                                                                                                      >  1:39195/1‑300 (MQ=255)                                                                                               aaGTTAGTAGGCAAAATTCAGGTGATCGCGCTGGATAGAAAGCACCAGTTTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTTAGGTTTATCACGAc                                                                                                                                                                                                     <  2:199046/300‑1 (MQ=255)                                                                                               aaGTTAGCATGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGAc                                                                                                                                                                                                     <  1:452536/300‑1 (MQ=255)                                                                                               aaGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGAc                                                                                                                                                                                                     >  1:300584/1‑300 (MQ=255)                                                                                                   tAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGTTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGcc                                                                                                                                                                                                 <  2:143114/300‑1 (MQ=255)                                                                                                   tAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGcc                                                                                                                                                                                                 <  1:511047/300‑1 (MQ=255)                                                                                                    aGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGGCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGcc                                                                                                                                                                                                 <  2:428341/299‑1 (MQ=255)                                                                                                         gCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGcc                                                                                                                                                                                           <  1:436248/300‑1 (MQ=255)                                                                                                            aaaTGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGACATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGaa                                                                                                                                                                                        >  2:81944/1‑300 (MQ=255)                                                                                                                               ggATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGgatga                                                                                                                                                                     >  1:568395/1‑300 (MQ=255)                                                                                                                                   aaaaaGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATaaa                                                                                                                                                                 <  1:574381/300‑1 (MQ=255)                                                                                                                                    aaaaGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAAt                                                                                                                                                                >  1:403048/1‑300 (MQ=255)                                                                                                                                      aaGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATgg                                                                                                                                                              <  1:624200/300‑1 (MQ=255)                                                                                                                                           ccAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACNACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGt                                                                                                                                                         >  2:230826/1‑300 (MQ=255)                                                                                                                                              gtgtGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGCTGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGAAAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGtaat                                                                                                                                                      >  2:654497/1‑297 (MQ=255)                                                                                                                                              gtgtGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCAt                                                                                                                                                      >  2:36749/1‑300 (MQ=255)                                                                                                                                              gtgtGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCAt                                                                                                                                                      >  1:397116/1‑300 (MQ=255)                                                                                                                                                gtgGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGNTCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAG‑ATGATAAATGGCATGTCAtttt                                                                                                                                                   >  1:517797/1‑300 (MQ=255)                                                                                                                                                gtgGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAACTCTGTCTTGCGTCTGATAAATGGCATGTCAttt                                                                                                                                                    >  1:206301/1‑300 (MQ=255)                                                                                                                                                gtgGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCAttt                                                                                                                                                    >  2:245728/1‑300 (MQ=255)                                                                                                                                                   gTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTTGCATGAGgattatcagattgcacttcccgttt                                                                                                                                                 >  1:248094/1‑278 (MQ=255)                                                                                                                                                   gTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTc                                                                                                                                                 >  1:51569/1‑300 (MQ=255)                                                                                                                                                   gTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCAAGACAGCCTGTGCAGAAAAATCTGGCGTGAGGATGATAAATGGCGTGTCATTTTTc                                                                                                                                                 >  1:51584/1‑300 (MQ=255)                                                                                                                                                   gTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGACAGCGTTGAAGTTTATCAAGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCAttttt                                                                                                                                                  >  2:246063/1‑299 (MQ=255)                                                                                                                                                   gTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGATATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCNCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTc                                                                                                                                                 >  1:516955/1‑300 (MQ=255)                                                                                                                                                            gTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGAc                                                                                                                                         >  1:195382/1‑299 (MQ=255)                                                                                                                                                             tttCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCAAGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGAc                                                                                                                                         >  2:367867/1‑298 (MQ=255)                                                                                                                                                                 aGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCt                                                                                                                                     >  1:503437/1‑298 (MQ=255)                                                                                                                                                                    aaaCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGa                                                                                                                                 <  1:72871/299‑1 (MQ=255)                                                                                                                                                                    aaaCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGa                                                                                                                                 <  2:639843/299‑1 (MQ=255)                                                                                                                                                                     aaCCCTGCGGCGGTCTGCTGGCACTGGACGCGCAGCGATCTTGTGTTCGCGATGGGCTGACGCTTCCTGGCGATGGGATCGCTAAGTCGCAGATTTTTAGTGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCGTGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGatggtgggga                                                                                                                                 <  2:364490/298‑10 (MQ=255)                                                                                                                                                                      aCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCa                                                                                                                              >  2:129436/1‑300 (MQ=255)                                                                                                                                                                          tGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAAc                                                                                                                            <  1:245728/298‑1 (MQ=255)                                                                                                                                                                          tGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACat                                                                                                                          <  1:417970/300‑1 (MQ=255)                                                                                                                                                                                gggctgctgGCACCTGAAGCTCAGCGATCTTTTATTCGCGATTTGCTTACGCTTCCTTTCGATGTGATCGCTAATCCACAGATTGTCAGTGTCAAGACTGTCGACGTAGCCGCATCGTTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCTACTTGTGGATGAAATCGCTGATCCCCGCCAGCGGTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCGGGCGTGAGGATGATAAATGGCATGTCATTTGGCGTGCAGACGGCTGGGAGCAACATAGTAc                                                                                                                     <  2:206301/296‑1 (MQ=255)                                                                                                                                                                                ggTCTGCTGGCACCGTACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAc                                                                                                                     <  2:316811/299‑1 (MQ=255)                                                                                                                                                                                ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTTTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAc                                                                                                                     <  2:316438/299‑1 (MQ=255)                                                                                                                                                                                ggTCTGCTGGCACCGGACGCACAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGGAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAATATATTAc                                                                                                                     <  2:676581/299‑1 (MQ=255)                                                                                                                                                                                ggTCTGCTGGCACCGGACGCACAGCGATCCTTTATTCGCGATGGGCTGACGCTTCCTGTCGATTTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAACTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCTTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAc                                                                                                                     <  2:316908/299‑1 (MQ=255)                                                                                                                                                                                 gTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccg                                                                                                                   <  2:633182/300‑1 (MQ=255)                                                                                                                                                                                  tCTGCTGGCACCAGACGCGCAGCGAGCTCTTATTCGCAATAGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCTAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAc                                                                                                                     <  2:316511/297‑1 (MQ=255)                                                                                                                                                                                   ctgctgGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccgc                                                                                                                  >  2:385288/1‑299 (MQ=255)                                                                                                                                                                                    tgctgGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccgccc                                                                                                                <  1:360401/300‑1 (MQ=255)                                                                                                                                                                                    tgctgGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccgcc                                                                                                                 <  1:225391/299‑1 (MQ=255)                                                                                                                                                                                     gctgGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccgccc                                                                                                                >  1:373816/1‑299 (MQ=255)                                                                                                                                                                                        ggCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGAATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTa                                                                                                            >  2:20516/1‑300 (MQ=255)                                                                                                                                                                                                  gcgcAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGt                                                                                                  <  1:357614/300‑1 (MQ=255)                                                                                                                                                                                                    gcAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGc                                                                                                <  1:686328/300‑1 (MQ=255)                                                                                                                                                                                                                          gggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGNTGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGt                                                                           >  2:524713/1‑299 (MQ=255)                                                                                                                                                                                                                           ggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTgc                                                                         >  1:672602/1‑300 (MQ=255)                                                                                                                                                                                                                           ggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCAATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGAGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGACCGATATCTGGTCGGTGCCGATGGTGCAAACTCGATGGTgc                                                                         >  2:178953/1‑300 (MQ=255)                                                                                                                                                                                                                                  gCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGAc                                                                   <  1:179734/299‑1 (MQ=255)                                                                                                                                                                                                                                   cTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAACTCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGTTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACAt                                                                 >  2:207054/1‑300 (MQ=255)                                                                                                                                                                                                                                     tCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACAtct                                                               <  1:444820/300‑1 (MQ=255)                                                                                                                                                                                                                                            gATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCATCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACGCGATGGTGCGGCGACATCTCTAccc                                                         <  2:342355/299‑1 (MQ=255)                                                                                                                                                                                                                                            gATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTAccc                                                         <  2:451848/299‑1 (MQ=255)                                                                                                                                                                                                                                            gATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCAGCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGACATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGTTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTAccc                                                         <  2:451836/299‑1 (MQ=255)                                                                                                                                                                                                                                                 gATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGatca                                                   >  1:674978/1‑300 (MQ=255)                                                                                                                                                                                                                                                 gATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTATACCCGGatca                                                   >  1:676122/1‑300 (MQ=255)                                                                                                                                                                                                                                                 gATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCAGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGatca                                                   >  1:676932/1‑300 (MQ=255)                                                                                                                                                                                                                                                   tCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGatcatc                                                 >  1:388216/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gatcttCAGCGTCAAAACCGTCGACGTCGCCACATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCTCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCTGCGACATCTCTACCCGGATCATCAAATCCGTAAata                                    <  2:431649/296‑1 (MQ=255)                                                                                                                                                                                                                                                                           tCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATcc                         <  1:433153/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                aCCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCcagcag                    <  2:409321/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                aCCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCcagcag                    <  1:387760/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCCAGCAGt                   >  1:55982/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                      gACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATNCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCCAGCAGTGGtt               <  2:568395/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 aTCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCcagcag                    <  2:101679/283‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  tCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCCAGCAGTGGTTCGCGGAGAAACAt  >  2:131516/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  tCGCTAACGCGTAACTACCAACGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGAAAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCCGGGAGCAACATATCACCGCCCGCTATCTAGCCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTATACCCGGATCATCAAATCCGTAAATATGTCGATATCCAGCAGTGGTTCGCGGAGAAACa   >  2:131277/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       GTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCCAGCAGTGGTTCGCGGAGAAACAT  >  NC_000913/3869343‑3869931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑