Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,241,458	G→A	T266I (ACC→ATC)	xylE ←	D‑xylose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,458	0	G	A	100.0%	254.0 / NA	74	T266I (ACC→ATC)	xylE	D‑xylose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/39);  total (35/39)

GTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC  >  NC_000913/4241163‑4241751                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      gtAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGAtttt                                                                                                                                                                                                                                                                                                   >  1:467684/1‑300 (MQ=255)             aCCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATc                                                                                                                                                                                                                                                                                       <  1:608526/300‑1 (MQ=255)                 aGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAGTGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCAAATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCa                                                                                                                                                                                                                                                                                     >  1:644582/1‑298 (MQ=255)                 aGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCNCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCa                                                                                                                                                                                                                                                                                     >  2:544042/1‑298 (MQ=255)                 aGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCa                                                                                                                                                                                                                                                                                     >  1:644576/1‑298 (MQ=255)                  ggCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGNACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAggg                                                                                                                                                                                                                                                                                  <  2:528979/300‑1 (MQ=255)                  ggCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGTAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAggg                                                                                                                                                                                                                                                                                  <  2:641643/300‑1 (MQ=255)                  ggCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAggg                                                                                                                                                                                                                                                                                  <  1:62777/300‑1 (MQ=255)                   gCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAggg                                                                                                                                                                                                                                                                                  <  2:491362/299‑1 (MQ=255)                     tAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGNGATATCCGTGCTGGCCCCCAGCGTTTTTAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAg                                                                                                                                                                                                                                                                                <  2:232973/299‑1 (MQ=255)                      aaaCATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCTTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGNGATATCCGTGCTGGCCCCCAGCGTTTTTAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGTCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAg                                                                                                                                                                                                                                                                                <  2:231567/298‑1 (MQ=255)                                 ttGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCt                                                                                                                                                                                                                                                                   >  1:224344/1‑300 (MQ=255)                                   gCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTc                                                                                                                                                                                                                                                                     >  1:677781/1‑296 (MQ=255)                                      aTTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTAc                                                                                                                                                                                                                                                               <  1:500011/299‑1 (MQ=255)                                      aTTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTAc                                                                                                                                                                                                                                                               <  2:625032/299‑1 (MQ=255)                                           gAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCt                                                                                                                                                                                                                                                          <  1:455442/299‑1 (MQ=255)                                                                 ggCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGACCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGAACAACGATTTTGCGGCCATGATCCAAGGAGTGTTTAATTTCCTGTACTGCATGCGTTGCAAGCGTGTTGCCCATa                                                                                                                                                                                                                                   >  2:421116/1‑300 (MQ=255)                                                                 ggCTTACGACCAAATTTATCCACCGTAATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGAACACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTAAGTTGCAAGCGTGTTGCCCATa                                                                                                                                                                                                                                   >  2:50416/1‑300 (MQ=255)                                                                    ttACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAAt                                                                                                                                                                                                                                 <  2:607637/299‑1 (MQ=255)                                                                                  aTCCACCGTCATAATTGCCAGAACGGTGAAGTTGAGGTTGATAACTTCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAAGC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAcc                                                                                                                                                                                                                  <  2:397832/300‑1 (MQ=255)                                                                                      accttcagaaTTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTc                                                                                                                                                                                                               <  2:37952/291‑1 (MQ=255)                                                                                      aCCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCTACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCATGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCtt                                                                                                                                                                                                                <  2:50609/298‑1 (MQ=255)                                                                                      aCCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCtt                                                                                                                                                                                                                <  1:745495/298‑1 (MQ=255)                                                                                      aCCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTc                                                                                                                                                                                                               <  2:35734/299‑1 (MQ=255)                                                                                       ccGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTcc                                                                                                                                                                                                              >  2:707851/1‑299 (MQ=255)                                                                                         gTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGNCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGcc                                                                                                                                                                                                           <  1:165340/300‑1 (MQ=255)                                                                                          tCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCt                                                                                                                                                                                                          >  2:715307/1‑300 (MQ=255)                                                                                                 tGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTctt                                                                                                                                                                                                    >  2:148158/1‑299 (MQ=255)                                                                                                 tGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGACAAACATCAGCAGACGACCACAGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCACAATTTTGCGCAGGATACCTTCCGCCTGTTctt                                                                                                                                                                                                    >  2:148147/1‑299 (MQ=255)                                                                                                 tGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCAGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGACTGTTctt                                                                                                                                                                                                    >  2:148214/1‑299 (MQ=255)                                                                                                 tGCCAGAACGGTGAAGGTGAGGTTGATAACTCAGACAATAATGGTCTGCAACAGCGCGATATCTGTGCTGGCCCCCAGCGTTTTGCACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACCAATTGCTGNAAGATGGAGAGCATTACGACTATTACAATC‑ACGCCCAAGCAAAACATCAGCAGACGACCACCGATTTTGTGGCCATGATACAGGGAGTGTTTAATTTCCTGTACTGACTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGACTGTTctt                                                                                                                                                                                                    >  2:534874/1‑299 (MQ=255)                                                                                                     aGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgct                                                                                                                                                                                                 <  1:435634/298‑1 (MQ=255)                                                                                                                                            gTCTGCAACAGCGCGATATCCGCGCTGGCCCCCAGCGTTTTGAACACTTACGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGGTGGAGNGCATTACGCCGATTACAATC‑ACGCCCACGCCANACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTTTTGCTTGCCGCGCGAAATCAGCCAGCGGGGACTTTCTTGAACGGta                                                                                                                                                        >  2:565533/1‑300 (MQ=255)                                                                                                                                                  aaCAGCGCGATATCTGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagc                                                                                                                                                  <  2:17468/300‑1 (MQ=255)                                                                                                                                                  aaCAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCTTAGTACAGCACCACATTGATGCCGACANATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagc                                                                                                                                                  <  1:529691/300‑1 (MQ=255)                                                                                                                                                  aaCAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACANATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagc                                                                                                                                                  <  1:529678/300‑1 (MQ=255)                                                                                                                                                  aaCAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGNTGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagc                                                                                                                                                  <  2:582978/300‑1 (MQ=255)                                                                                                                                                  aaCAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGCGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagc                                                                                                                                                  <  2:648962/300‑1 (MQ=255)                                                                                                                                                  aaCAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagc                                                                                                                                                  <  2:709142/300‑1 (MQ=255)                                                                                                                                                  aaCAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagc                                                                                                                                                  <  1:82924/300‑1 (MQ=255)                                                                                                                                                  aaCAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCAAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagc                                                                                                                                                  <  1:455654/300‑1 (MQ=255)                                                                                                                                                      gcgcGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCAGCGCGACATCAGACAGCGAGGACTTTCTGGCACGGTATACAgcagca                                                                                                                                              >  2:309501/1‑300 (MQ=255)                                                                                                                                                        gcgATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCAt                                                                                                                                             <  1:606689/299‑1 (MQ=255)                                                                                                                                                                             aGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGa                                                                                                                         <  1:694999/298‑1 (MQ=255)                                                                                                                                                                                gTTTTGAACCCTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTACGCCTGTTCTTGCTTGCCGCGCGAAATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACAt                                                                                                                    >  1:473930/1‑300 (MQ=255)                                                                                                                                                                                  tttGAACACTTCCGGCGNGTAGTACAGCACCACATTNATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCNGTGCAGGGATACATTc                                                                                                                  <  1:617355/300‑1 (MQ=255)                                                                                                                                                                                          cTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCCCGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCAGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCa                                                                                                           >  2:741906/1‑299 (MQ=255)                                                                                                                                                                                          cTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCaa                                                                                                          >  2:741455/1‑300 (MQ=255)                                                                                                                                                                                                  gcgTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATaa                                                                                                  >  1:65951/1‑300 (MQ=255)                                                                                                                                                                                                  gcgTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATaa                                                                                                  >  2:181139/1‑300 (MQ=255)                                                                                                                                                                                                    gtagtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGNCCATAATTTTGCGCAGGATACCTTCCGCCTGTNCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAAc                                                                                                 <  2:559136/299‑1 (MQ=255)                                                                                                                                                                                                     tagtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCNGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGc                                                                                               >  2:537191/1‑300 (MQ=255)                                                                                                                                                                                                     tagtaCAGAACAACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCNGTACTGCCTGCGTTGCAAGCGTGTTGCCCATAATTTTGCGCCGGATACCTTCAGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTACGAACAGCAGTACAGGGATACATTCAGAggaaa                                                                                                          >  2:537731/1‑286 (MQ=255)                                                                                                                                                                                                       gtaCCGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCa                                                                                             >  1:588959/1‑300 (MQ=255)                                                                                                                                                                                                       gtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACCATC‑ACGCCCACGCCCACCATCAGCAGGCGACTACCGATTTTGCGGCCATGATCCAGGGCGTGTTTCATTTCCTGTACTGCCTGAGTTGCCAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATTAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTATACGGCAAACATATaaagcaa                                                                                             >  2:69657/1‑295 (MQ=255)                                                                                                                                                                                                       gtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCNAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCa                                                                                             >  1:142630/1‑300 (MQ=255)                                                                                                                                                                                                       gtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCa                                                                                             >  1:69442/1‑300 (MQ=255)                                                                                                                                                                                                       gtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCAAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATAGAACGCCa                                                                                             >  2:65218/1‑300 (MQ=255)                                                                                                                                                                                                       gtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTATTCTTGCTTGCCGCGCGAAATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCa                                                                                             >  1:466940/1‑300 (MQ=255)                                                                                                                                                                                                       gtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTAAGCCGATT‑CAGCCTGTGCCTATGCAAAACATCAGCAGATGAACACCGATTTTGCGGCCATAATc                                                                                                                                                                                                                                                                                       >  1:338824/1‑114 (MQ=255)                                                                                                                                                                                                            gCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGt                                                                                        >  1:83179/1‑300 (MQ=255)                                                                                                                                                                                                                   aTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTAt                                                                                  <  2:460992/299‑1 (MQ=255)                                                                                                                                                                                                                       aTGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGNCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTcagc                                                                             <  2:591307/300‑1 (MQ=255)                                                                                                                                                                                                                             aCAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTg                                                                       <  2:467684/300‑1 (MQ=255)                                                                                                                                                                                                                                       ggAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCAcc                                                               >  1:172558/1‑298 (MQ=255)                                                                                                                                                                                                                                            atgtagagCACTACGCCGATTACAACC‑ACGCCCAAGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGATTGTTTAATTTCCTTTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGGTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGTACGGTATACAGCAGCATTAAGAACAGCAATGCAGGGAGGCATTTCGAGGCAAACATATAACGCCAGCCGGCAGTATTCAGCCAGCTGGCATCACCGGAACgg                                                        <  1:315779/296‑1 (MQ=255)                                                                                                                                                                                                                                                      ttACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTNAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAAt                                              <  1:544042/300‑1 (MQ=255)                                                                                                                                                                                                                                                        aCGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGTCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAg                                            <  2:494902/300‑1 (MQ=255)                                                                                                                                                                                                                                                               ttACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAGAATAGTTTACGc                                     <  1:447013/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              acgccAAACATCAGCAGACTACCACCGATTTTTCGGCCATGATCCAGTGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGNCGCGCGACATCAGCCAGCGAGGACTTTCTTGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCTAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAACTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAg                        <  2:222904/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gcagACGACCACCGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAaataa          >  1:716130/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  accaccGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCAGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCAGTCAGTATTCAGCCAGCTGGCATCAACGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCaaa   >  2:1376/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  accaccGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCACATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCCTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTAAGTATTTAGCCAGCTGGTATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGAACGAAAATAATCGCa     >  2:1001/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                     accGATTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAc  <  2:683144/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      GTAAAACGCGGTACCGAGGCTAAACATACCGATTGCCATTCCGAGTGCGCCGATAATTTGCAGTGGCTTACGACCAAATTTATCCACCGTCATAATTGCCAGAACGGTGAAGGTGAGGTTGATAACTCCGACAATAATGGTCTGCAACAGCGCGATATCCGTGCTGGCCCCCAGCGTTTTGAACACTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATC‑ACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC  >  NC_000913/4241163‑4241751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑