Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,193,996	T→G	D70A (GAT→GCT)	thiE ←	thiamine phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,193,996	0	T	G	100.0%	231.5 / NA	63	D70A (GAT→GCT)	thiE	thiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/36);  total (27/36)

GTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTT  >  NC_000913/4193723‑4194289                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      gTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGTACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTtata                                                                                                                                                                                                                                                                              >  1:713995/1‑299 (MQ=255)    ggATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAATGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAgcgg                                                                                                                                                                                                                                                                          <  1:91718/300‑1 (MQ=255)     gATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAgcgg                                                                                                                                                                                                                                                                          <  2:48605/299‑1 (MQ=255)      aTAATCCGCCAGTTGCTCAGCATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAATAGGGGCGTGCTGCCAGCGCTACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAgcgg                                                                                                                                                                                                                                                                          <  2:257127/298‑1 (MQ=255)                 gTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCaa                                                                                                                                                                                                                                                             >  1:456230/1‑300 (MQ=255)                    gCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAAtt                                                                                                                                                                                                                                                           >  1:479434/1‑299 (MQ=255)                                         gTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCa                                                                                                                                                                                                                                     >  1:110821/1‑300 (MQ=255)                                                    tGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGANTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCAt                                                                                                                                                                                                                            <  2:583873/298‑1 (MQ=255)                                                          gCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGa                                                                                                                                                                                                                    >  1:304749/1‑300 (MQ=255)                                                                      tGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTATTCATCGCGCCGATCttttctgcgt                                                                                                                                                                                                        >  2:137981/1‑293 (MQ=255)                                                                       gtttggtttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGNTAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTa                                                                                                                                                                                                       >  2:590075/1‑300 (MQ=255)                                                                       gtttggtttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTACCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTa                                                                                                                                                                                                       >  2:629925/1‑300 (MQ=255)                                                                              ttgCGTCGTGAACACATGTCCCAGCGCGATATAAGAGGTGCGTGCGGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCTTAGCTGgag                                                                                                                                                                                                <  2:1025/300‑1 (MQ=255)                                                                              ttgCGTCGGGAACACATNTCCCAGCGCGATATAAGANGGGCGTGCTACCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTNTGATGCGTAGCTGgag                                                                                                                                                                                                <  1:629925/300‑1 (MQ=255)                                                                              ttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGgag                                                                                                                                                                                                <  1:639548/300‑1 (MQ=255)                                                                                      ggAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtacgta                                                                                                                                                                                        <  2:124208/300‑1 (MQ=255)                                                                                       gAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtacgtac                                                                                                                                                                                       >  1:189269/1‑300 (MQ=255)                                                                                       gAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGACCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGAGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCAAGACATCGGCTTCCACCTATTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgt                                                                                                                                                                                             >  2:377449/1‑294 (MQ=255)                                                                                       gAACACATGTCCAAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGACCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtacgcac                                                                                                                                                                                       >  2:377438/1‑297 (MQ=255)                                                                                        aaCACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCTCTACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGtacgtacg                                                                                                                                                                                      <  2:346756/300‑1 (MQ=255)                                                                                           acaTGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGcc                                                                                                                                                                                    <  2:364090/299‑1 (MQ=255)                                                                                                          atataatcggggCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGNCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGa                                                                                                                                                                       <  2:581527/289‑1 (MQ=255)                                                                                                          atatAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGt                                                                                                                                                                    >  1:720794/1‑300 (MQ=255)                                                                                                             taAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATNGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTc                                                                                                                                                                  >  2:575151/1‑299 (MQ=255)                                                                                                                       gTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGt                                                                                                                                                        <  2:741550/299‑1 (MQ=255)                                                                                                                        tgctgcCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTAc                                                                                                                                                      <  2:200415/300‑1 (MQ=255)                                                                                                                           tgcCAGCGCGACGTTGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTTAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGc                                                                                                                                                    <  2:167124/299‑1 (MQ=255)                                                                                                                           tgcCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGc                                                                                                                                                    <  1:291346/299‑1 (MQ=255)                                                                                                                                    gACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCcaccac                                                                                                                                          <  1:295244/300‑1 (MQ=255)                                                                                                                                    gACGTCGATTTCCAGATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCcaccac                                                                                                                                          <  2:162419/300‑1 (MQ=255)                                                                                                                                           aTTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTAc                                                                                                                                   <  1:657057/300‑1 (MQ=255)                                                                                                                                                 atatCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGcctgtctcttatacacatctcgc                                                                                                                              <  1:590075/299‑23 (MQ=255)                                                                                                                                                 atatCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTcc                                                                                                                              <  2:719235/299‑1 (MQ=255)                                                                                                                                                      gTCATGTGTCTAAACGCCCAGCCGCAGGCCTACCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAAc                                                                                                                         <  2:354529/299‑1 (MQ=255)                                                                                                                                                      gTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAAc                                                                                                                         <  1:512742/299‑1 (MQ=255)                                                                                                                                                      gTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAAc                                                                                                                         >  2:695981/1‑299 (MQ=255)                                                                                                                                                      gTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCACCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAAc                                                                                                                         <  2:44159/299‑1 (MQ=255)                                                                                                                                                      gTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATAGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAAc                                                                                                                         <  2:354009/299‑1 (MQ=255)                                                                                                                                                           gtgtCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACCCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCAACGGGTACAGTCCTGAACGaaaa                                                                                                                    >  1:292032/1‑299 (MQ=255)                                                                                                                                                                    cGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATNGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACagga                                                                                                           >  2:540649/1‑299 (MQ=255)                                                                                                                                                                          gCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGc                                                                                                                                                    <  1:476027/252‑1 (MQ=255)                                                                                                                                                                          gCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGc                                                                                                                                                    >  2:476027/1‑252 (MQ=255)                                                                                                                                                                                ggCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCt                                                                                              >  2:606717/1‑300 (MQ=255)                                                                                                                                                                                     gCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATGCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATAc                                                                                         <  2:633845/300‑1 (MQ=255)                                                                                                                                                                                     gCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATAc                                                                                         <  1:689507/300‑1 (MQ=255)                                                                                                                                                                                     gCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAACCAGGCTGATAc                                                                                         <  1:689067/300‑1 (MQ=255)                                                                                                                                                                                        gcgcgGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACAt                                                                                       <  1:176241/299‑1 (MQ=255)                                                                                                                                                                                            ggATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCa                                                                                     <  2:648668/297‑1 (MQ=255)                                                                                                                                                                                            ggATGGCATGTAGATTGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTGTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGGTACATCa                                                                                     <  2:648728/297‑1 (MQ=255)                                                                                                                                                                                                 gCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcct                                                                             >  1:256866/1‑300 (MQ=255)                                                                                                                                                                                                        gaTCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGNTCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCAAGCTTCCTCCTTAc                                                                       >  2:581950/1‑299 (MQ=255)                                                                                                                                                                                                        gaTCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCATTa                                                                        >  1:229635/1‑298 (MQ=255)                                                                                                                                                                                                         aTCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGc                                                                     <  1:553599/300‑1 (MQ=255)                                                                                                                                                                                                         aTCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCGGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCACAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCTTAGCTGGAGAGTACGTACACCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGc                                                                     <  2:204209/300‑1 (MQ=255)                                                                                                                                                                                                                                              ccATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCTAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGACTGATACATCACGCTTAATCCTTACGCAGGTAAATTTCTCCGCCTCTGGCCCGGAAGt                                      >  2:134493/1‑294 (MQ=255)                                                                                                                                                                                                                                              ccATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTcc                                 >  1:181306/1‑299 (MQ=255)                                                                                                                                                                                                                                                 tACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACa                             <  1:217514/300‑1 (MQ=255)                                                                                                                                                                                                                                                     ccTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATc                         >  1:453049/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ccTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTAATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCAACGGGTACAGTCCTGAACGAAGAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCATCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTTTACGAAATATc                         >  2:264588/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gCTTGATCGCCAGCCGCCAGGAAGCATTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCTGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTACATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAACCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCGCCGACATATCCGcc                     <  2:441577/298‑1 (MQ=255)                                                                                                                                                                                                                                                                aTCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCa              >  2:56294/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gccAGTAAGCGTTGATNAACAATCGCGCGTTATAGNGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCCCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCNATTCCCATTTCAATAg     <  1:636348/299‑1 (MQ=255)                                                                                                                                                                                                                                                                             aGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTATTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCCGGTAGATTTCTCCGCCTATGGCAAGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGttt  >  2:83189/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      GTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATAGTTT  >  NC_000913/4193723‑4194289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑