Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,165,712	C→A	A95A (GCC→GCA)	murI →	glutamate racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,165,712	0	C	A	98.9%	300.7 / NA	93	A95A (GCC→GCA)	murI	glutamate racemase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (52/40);  total (53/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTATGGCTACCAAACTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCCAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCTACGCCCGTGGTTAAGAATGA  >  NC_000913/4165424‑4166007                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                            gTCTATGGCTACCAAACTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCACGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGtt                                                                                                                                                                                                                                                                                                    >  1:405505/1‑298 (MQ=255) gTCTATGGCTACCAAACTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAATGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTc                                                                                                                                                                                                                                                                                                  >  1:221976/1‑300 (MQ=255) gTCTATGGCTACCAAACTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTc                                                                                                                                                                                                                                                                                                  >  1:425098/1‑300 (MQ=255) gTCTATGGCTACCAAACTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTAAATTTATGCTTTCGATAACGTCGCTTTCCAGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCATTTc                                                                                                                                                                                                                                                                                                  >  2:34182/1‑300 (MQ=255)                 cTGCAGGACGGGAATACACCTTGTCTGGAAGCTACACCTTCTGAACCACGTCCCAACGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGCATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCACTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTc                                                                                                                                                                                                                                                                                             >  2:412215/1‑289 (MQ=255)                   gCAGGACGGGACTACACCTTGTCTGGCAGCTACACCTTCTGAACCACTTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCTGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCa                                                                                                                                                                                                                                                                                        <  2:441338/292‑1 (MQ=255)                           ggAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAANAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTc                                                                                                                                                                                                                                                                         >  2:240794/1‑299 (MQ=255)                           ggAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTc                                                                                                                                                                                                                                                                         >  2:335892/1‑299 (MQ=255)                           ggAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTAAATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCCAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTc                                                                                                                                                                                                                                                                         >  2:520693/1‑299 (MQ=255)                           ggAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCAACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTATTACCGGATCTCAATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATAGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTc                                                                                                                                                                                                                                                                         >  2:336023/1‑299 (MQ=255)                            gAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGa                                                                                                                                                                                                                                                                       >  1:382599/1‑300 (MQ=255)                                 cacCTTGTCTGGCAGCTACACCCTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGCCGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGCAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTACGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTAACTTCCTGAATTAAGCGAAAAGTTCGaatccc                                                                                                                                                                                                                                                                  >  2:404774/1‑295 (MQ=255)                                          tGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACTAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGACAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGgttg                                                                                                                                                                                                                                                            <  2:200260/297‑1 (MQ=255)                                             cAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGgt                                                                                                                                                                                                                                                       >  1:515665/1‑299 (MQ=255)                                             cAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGgt                                                                                                                                                                                                                                                       >  1:515745/1‑299 (MQ=255)                                             cAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTACTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGgt                                                                                                                                                                                                                                                       >  2:349049/1‑299 (MQ=255)                                              aGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGgtgt                                                                                                                                                                                                                                                     >  1:277334/1‑300 (MQ=255)                                                                acttCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAAcc                                                                                                                                                                                                                                   <  1:395815/297‑1 (MQ=255)                                                                aCGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAAcc                                                                                                                                                                                                                                   >  1:2337/1‑300 (MQ=255)                                                                aCGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGANCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTAGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAAcc                                                                                                                                                                                                                                   <  1:148021/300‑1 (MQ=255)                                                                aCGTCCCACCGTGCTGGTGGTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAAcc                                                                                                                                                                                                                                   <  1:208784/300‑1 (MQ=255)                                                                  gTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACct                                                                                                                                                                                                                                  >  1:30361/1‑299 (MQ=255)                                                                          gTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGt                                                                                                                                                                                                                         >  1:9336/1‑300 (MQ=255)                                                                                 tgtTTGACTCCGGCGTCGGTGGGTTGT‑GGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGc                                                                                                                                                                                                                 <  1:400284/300‑1 (MQ=255)                                                                                            ggCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCAt                                                                                                                                                                                                        <  1:447644/299‑1 (MQ=255)                                                                                                tCGGTGGGTTGTCGGGCTATGACGAGATCCGGAATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCCGACgtc                                                                                                                                                                                                                 <  2:659008/286‑3 (MQ=255)                                                                                                  ggTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGa                                                                                                                                                                                                 >  1:491551/1‑300 (MQ=255)                                                                                                     gggTTGTCGGGTTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGTTGGCAATTGTCANCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTa                                                                                                                                                                                              <  2:235356/300‑1 (MQ=255)                                                                                                       gTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCACGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACt                                                                                                                                                                                            >  2:709847/1‑300 (MQ=255)                                                                                                         tGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTAAATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACt                                                                                                                                                                                            >  1:573229/1‑298 (MQ=255)                                                                                                             ggTCTATTACTAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAAc                                                                                                                                                                                      <  2:454979/300‑1 (MQ=255)                                                                                                                       gagaTCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAAc                                                                                                                                                                             <  1:382765/299‑1 (MQ=255)                                                                                                                        agaTCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACa                                                                                                                                                                            >  1:212181/1‑299 (MQ=255)                                                                                                                           tCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTa                                                                                                                                                                       <  2:659048/300‑1 (MQ=255)                                                                                                                             cGGCATCTCTTCCCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTaaa                                                                                                                                                                     >  1:569686/1‑300 (MQ=255)                                                                                                                                 aTCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGt                                                                                                                                                                  <  1:281041/299‑1 (MQ=255)                                                                                                                                  tctcTTACCGGATCTCCACTACATTTATGCTTTCGATAACGCCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTTTTGAGCGATTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGNGCTGGCTGTGGTCGCTTGCAGCACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAATTCGACTTCCCGTTTGTTGGTGTCGGGCCGGCGATTAAGCCTACTGCACGTCTGACTGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGt                                                                                                                                                                  <  2:233889/298‑1 (MQ=255)                                                                                                                                   ctctTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTACTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCt                                                                                                                                                               >  2:466030/1‑300 (MQ=255)                                                                                                                                    tctTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCt                                                                                                                                                               <  1:137922/299‑1 (MQ=255)                                                                                                                                        aCCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATAc                                                                                                                                                          <  1:479082/300‑1 (MQ=255)                                                                                                                                        aCCGGATCTCCATTACANTTATGCTTTCGATAACGTNGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGACCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAN‑TTAAACGTTCTTATAc                                                                                                                                                          <  1:634693/300‑1 (MQ=255)                                                                                                                                                tcCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTTGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGc                                                                                                                                                  <  2:493708/300‑1 (MQ=255)                                                                                                                                                tcCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGc                                                                                                                                                  <  1:429551/300‑1 (MQ=255)                                                                                                                                                tcCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGNGGCAATTGTAACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGc                                                                                                                                                  <  1:540704/300‑1 (MQ=255)                                                                                                                                                tcCATTACATTTATGCTTTCGATAACGTCGCTTTCCCATATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGc                                                                                                                                                  <  2:364784/300‑1 (MQ=255)                                                                                                                                                 ccATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCt                                                                                                                                                 >  1:497555/1‑300 (MQ=255)                                                                                                                                                    ttACATTTATGCTTTCGATGACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGATTCAAGAACGTTATC‑‑CCCTTGCGCTGGCGGTGGTCACTTGCAACGCTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGc                                                                                                                                                  <  2:365025/296‑1 (MQ=255)                                                                                                                                                        aTTTGTGCTTTCGATGACCTCGCTTTCCCTTAGTGCGAAAAAAGCGAAGCGTGTATTGTTGATCGAGTGTTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGNGCTGGCTGTGGTCACTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGt                                                                                                                                                                  <  2:234261/276‑1 (MQ=255)                                                                                                                                                                     aTAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTaatg                                                                                                                             <  1:100668/300‑1 (MQ=255)                                                                                                                                                                             gCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCa                                                                                                                      >  1:701734/1‑299 (MQ=255)                                                                                                                                                                             gCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTAAGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCa                                                                                                                      >  1:701518/1‑299 (MQ=255)                                                                                                                                                                                ttCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTNGCAACACTGCAAGTACCGTTTAACTTCCTGCANTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTAGATAATGACTGCCAGATa                                                                                                                  >  2:559199/1‑300 (MQ=255)                                                                                                                                                                                      tATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAAt                                                                                                             <  1:418921/299‑1 (MQ=255)                                                                                                                                                                                                         tgTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGACATGCTGGGCTCG‑G‑CAGAG‑At                                                                                        >  2:332622/2‑298 (MQ=255)                                                                                                                                                                                                         cgTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGTAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTAAGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCACGAGCTGATCGCGCGTTTAGCTAATGAATGCCAGATAGAAATGCTGGGCt                                                                                                     >  2:332376/1‑288 (MQ=255)                                                                                                                                                                                                         cgTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTACAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTCCGCTAATGAATGCCAGATAGAAATGCTGGGCTAG‑G‑CAGAG‑At                                                                                        >  2:333018/1‑298 (MQ=255)                                                                                                                                                                                                         cgTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTACAACACTGCCAGTACCGTTTAACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATCGTCGGATTACTGGCAACCCGCGGACCAG‑TTACACGTTCTTATACTCATGAGCTGATCGCGCGTTTAGCTAATGAATGCCAGATAGAAATGCTGGGCTc                                                                                                    >  2:332388/1‑289 (MQ=255)                                                                                                                                                                                                                 gttgAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CATAG‑ATGGTTGAGTTg                                                                              <  1:385240/300‑1 (MQ=255)                                                                                                                                                                                                                 gttgAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTg                                                                              <  1:384996/300‑1 (MQ=255)                                                                                                                                                                                                                 gttgAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGtt                                                                               >  1:709504/1‑299 (MQ=255)                                                                                                                                                                                                                        gAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTgaa                                                                        >  2:602395/1‑299 (MQ=255)                                                                                                                                                                                                                         aGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTgaagc                                                                      <  1:8592/300‑1 (MQ=255)                                                                                                                                                                                                                                   aTTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTTTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACAt                                                            <  2:399491/300‑1 (MQ=255)                                                                                                                                                                                                                                   aTTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCGCG‑G‑CGGAG‑GGGGTTGAGTTGGCTgaagtggggtgatgg                                                            <  2:425098/300‑12 (MQ=255)                                                                                                                                                                                                                                   aTTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCGTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACAt                                                            <  1:661843/300‑1 (MQ=255)                                                                                                                                                                                                                                     tGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGNCGGATTACTGGCAACCCGCGGAACAG‑TTAAACNTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATgg                                                          <  2:573479/300‑1 (MQ=255)                                                                                                                                                                                                                                               gTGCAAGAACGTTATCNGCCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACGGGTTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑GGCAGAGGNTGGTTGAGTTGGCTgaaangcaaagctacaggggc                                                      >  1:620145/1‑281 (MQ=255)                                                                                                                                                                                                                                               gTGCAAGAACGTTATCNGCCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACGGGTTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCAAGATAGAAATGCTGGGCTCGCG‑CAGAGGNTGGTTGAGTTGGCtaaaanaaaaaccaactcggcca                                                     >  1:620401/1‑278 (MQ=255)                                                                                                                                                                                                                                               gTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGtt                                                >  2:627611/1‑300 (MQ=255)                                                                                                                                                                                                                                               gTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGtt                                                >  1:396143/1‑300 (MQ=255)                                                                                                                                                                                                                                               gTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGtt                                                >  1:395612/1‑300 (MQ=255)                                                                                                                                                                                                                                                    aGAACGTTCTC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCTGAACAG‑TTAAACGTTCCTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATTCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATg                                                  <  1:231340/293‑1 (MQ=255)                                                                                                                                                                                                                                                       aCGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTAAGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGa                                        >  2:455649/1‑300 (MQ=255)                                                                                                                                                                                                                                                        cGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCAAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTAGAt                                       >  2:400847/1‑300 (MQ=255)                                                                                                                                                                                                                                                        cGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGATTGCAACACTGCAAGTACCGTTTCACTTCCTGCTTTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑GTGGTTGAGTTGGCTGCAGCGACGCTACATGGCGAAGAt                                                   >  2:731629/1‑288 (MQ=255)                                                                                                                                                                                                                                                             tC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGTCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACt                                  <  2:492478/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 cccTTGCGCTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTaa                                >  2:607785/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     tgtgctggttgTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCTGGTTGTTGGTGTCGTGCCGTCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACg                            <  2:405505/297‑1 (MQ=255)                                                                                                                                                                                                                                                                      gcgcTGGCTGTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGt                           <  2:497555/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tggctgTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATcc                       <  1:34182/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           ggctgTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCAAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACTTATCCt                      >  2:205749/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           ggctgTGGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCAGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCt                      >  2:204792/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                tgGTCGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCTACGcc                 <  1:34486/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    cGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCTACGCCCGt              >  1:273173/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                    cGCTTGCAACACTGCAAGTACCGTTTCACTTCCTGCATTAAGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCTACGCCCGt              >  1:273315/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                       ttGCAACACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCTACGCCCGTGGtt          >  1:739238/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           aaCACTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCTACGCCCGTGGTTAAg       <  2:30361/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                            acacTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGATTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTATCTGGATGCACTCAACCGTATCCTACGCCCGt              >  2:104769/1‑291 (MQ=255)                                                                                                                                                                                                                                                                                            acacTGCAAGTACCGTTTCACTTCCTGCATTAAGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTATGGCTGCACTAAAACGTATCCTACGCCCATGGTTAAGa      >  2:105783/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                              acTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCTACGCCCGTGGTTAAGAAt    <  2:472793/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                               cTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCTACGCCCGTGGTTAAGGATGa  >  2:328808/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               cTGCAAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCAGGTTGTTGGTGTCATGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTATTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ACGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCTACGCCCGTGGTTAAGAATGa  >  2:411469/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               cTGCAAGTACCGTTTCACTTCCTGCATTAAGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCAAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGCAGATGTTTCTCTGGATGCACTAAAACGTATCCTa                     >  2:315301/1‑281 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                            GTCTATGGCTACCAAACTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTTTGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCGATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCGGTGCAAGAACGTTATC‑‑CCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCCAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTTCGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGGCAACCCGCGGAACAG‑TTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTGGGCTCG‑G‑CAGAG‑ATGGTTGAGTTGGCTGAAGCGAAGCTACATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCTACGCCCGTGGTTAAGAATGA  >  NC_000913/4165424‑4166007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑