Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,730,560	C→T	W69* (TGG→TAG)	xylA ←	xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,730,560	0	C	T	99.0%	314.1 / NA	95	W69* (TGG→TAG)	xylA	xylose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (49/45);  minor base G (0/1);  total (49/46)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCCGCCCAGCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAGTTGCTCAATTATGTTATTT  >  NC_000913/3730264‑3730853                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      ttGCCGCCCAGCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACg                                                                                                                                                                                                                                                                                                    <  2:745202/300‑1 (MQ=255)           gCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTTCTGCTACGGACGATTaaa                                                                                                                                                                                                                                                                                          >  1:640489/1‑300 (MQ=255)           gCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCCGCTACGGACGATTaaa                                                                                                                                                                                                                                                                                          >  1:248040/1‑300 (MQ=255)           gCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCNTGCTTGCCTGCCAGGACATCAACCATTTGCGCNAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGACAGTGCCTCACCAGGCTGCTGCTACGGACGAttcaa                                                                                                                                                                                                                                                                                          >  2:573354/1‑297 (MQ=255)           gCTGAAGACTTCAGGATCTGGGTTCGTCGCAGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTAACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAGTTATTGATGTACTCTTTTAACGACGCGCACTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGAAATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACAATTaa                                                                                                                                                                                                                                                                                           >  2:180919/1‑299 (MQ=255)                 gACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAATGcccc                                                                                                                                                                                                                                                                                    >  2:338042/1‑300 (MQ=255)                 gACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGCGCATAAACAGGCTGCTGCTACGGACGATTAAACGcccc                                                                                                                                                                                                                                                                                    >  2:338314/1‑300 (MQ=255)                 gACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCACCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGGAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTCTACGCTTAGCCAACGCCAGTGTCTAACTAGGCTGCTGCTACGGACGATTAGACGccc                                                                                                                                                                                                                                                                                     >  2:338034/1‑299 (MQ=255)                   cTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATNGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCAACAGGCTGCTGCTACGGACGATTAAACGCCCCca                                                                                                                                                                                                                                                                                  >  2:530037/1‑300 (MQ=255)                        ggATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAANGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCa                                                                                                                                                                                                                                                                             >  1:144469/1‑300 (MQ=255)                              gggTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACata                                                                                                                                                                                                                                                                       <  1:499871/300‑1 (MQ=255)                                gTTCGTCGCCGCACCCGCGCCTTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCTCAAAATTATTGATGTACTCTNTTAACGACGCGCCCTCAGTGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATc                                                                                                                                                                                                                                                                     <  2:231853/300‑1 (MQ=255)                                gTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATc                                                                                                                                                                                                                                                                     <  2:441646/300‑1 (MQ=255)                                           cACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCNTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTcc                                                                                                                                                                                                                                                          >  1:534527/1‑300 (MQ=255)                                                   ccGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGaa                                                                                                                                                                                                                                                   >  1:440401/1‑299 (MQ=255)                                                     ttagtgagggttgtgaaaGCAGTTGGCCGTTCCCCACATCAGTTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAgg                                                                                                                                                                                                                                                 <  1:272733/284‑1 (MQ=255)                                                     gTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGTGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTTTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAgg                                                                                                                                                                                                                                                 <  1:200276/299‑1 (MQ=255)                                                     gTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAgg                                                                                                                                                                                                                                                 <  1:199874/299‑1 (MQ=255)                                                       aGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGgtgt                                                                                                                                                                                                                                              >  1:440328/1‑300 (MQ=255)                                                                ttGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGTACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGc                                                                                                                                                                                                                                     <  1:705699/300‑1 (MQ=255)                                                                ttGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGTAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGc                                                                                                                                                                                                                                     >  1:13554/1‑300 (MQ=255)                                                                  gTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCAAGTAGCa                                                                                                                                                                                                                                    >  2:695419/1‑299 (MQ=255)                                                                                 gTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCaa                                                                                                                                                                                                                     >  1:130675/1‑299 (MQ=255)                                                                                    ccccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGNAAACATCCACATCGTGGAAGCAATAAAATGGCNCATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCAAGTAGCAGGCGGCAAAACGCAAGTGc                                                                                                                                                                                                                 >  2:560907/1‑300 (MQ=255)                                                                                    ccccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCTACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCACACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCAAGTAGCAGGCGGCAAAACGCAAGt                                                                                                                                                                                                                   >  2:597285/1‑298 (MQ=255)                                                                                    ccccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCAGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGc                                                                                                                                                                                                                 >  2:526660/1‑300 (MQ=255)                                                                                    ccccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCAACAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCAGTTCAAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGc                                                                                                                                                                                                                 >  2:309590/1‑300 (MQ=255)                                                                                    caccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGc                                                                                                                                                                                                                 >  1:642589/3‑300 (MQ=255)                                                                                    caccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCAGTTCCAGCAGAAGGTGTGCAAGTAGCAGGCGGCAAAACGCAAGt                                                                                                                                                                                                                   >  2:309575/3‑298 (MQ=255)                                                                                         cagcagCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCACACACCAAACATATCCGCCACGTTCAAGCGGAAGGTGTGCCAGTAGTAGGTGGCAACACGCAAGTGCTCtt                                                                                                                                                                                                             >  1:401981/1‑299 (MQ=255)                                                                                             agcTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCAt                                                                                                                                                                                                         <  2:621360/299‑1 (MQ=255)                                                                                                ttCACGCCGCTCTCTTCTTGCTTGCCTGCCAGTACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCTTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGc                                                                                                                                                                                                     <  2:350366/300‑1 (MQ=255)                                                                                                ttCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCAAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGc                                                                                                                                                                                                     <  1:564854/300‑1 (MQ=255)                                                                                                ttCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTCTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCATAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATAc                                                                                                                                                                                                       <  2:513064/298‑1 (MQ=255)                                                                                                ttCACGCCGCTCTCTTCGTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACTACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTNGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCCGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGc                                                                                                                                                                                                     <  2:248040/300‑1 (MQ=255)                                                                                                ttCACGCCGCTCTCGTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGTGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCGGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGc                                                                                                                                                                                                     <  2:686502/300‑1 (MQ=255)                                                                                                ttCACGCCGCTCTCGTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACACAAGTGCTCTTCCATACGc                                                                                                                                                                                                     <  2:370910/300‑1 (MQ=255)                                                                                                        gcTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTAccc                                                                                                                                                                                               <  1:135915/298‑1 (MQ=255)                                                                                                        gcTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCNCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTAccc                                                                                                                                                                                               <  1:152959/298‑1 (MQ=255)                                                                                                            tcttctTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGNAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACAcc                                                                                                                                                                                         <  1:531740/300‑1 (MQ=255)                                                                                                            tcttctTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACAcc                                                                                                                                                                                         <  1:439886/300‑1 (MQ=255)                                                                                                             cttcttGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAacaaca                                                                                                                                                                                        >  1:471063/1‑297 (MQ=255)                                                                                                             cttcttGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCa                                                                                                                                                                                        >  1:577152/1‑300 (MQ=255)                                                                                                             cttcttGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCAAACACCa                                                                                                                                                                                        >  2:321456/1‑300 (MQ=255)                                                                                                                          tgccAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGAt                                                                                                                                                                            <  1:614068/299‑1 (MQ=255)                                                                                                                                 aCATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCNTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTAGGGATTGTAGTGa                                                                                                                                                                    >  2:579709/1‑300 (MQ=255)                                                                                                                                  cATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTGAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGAc                                                                                                                                                                   >  1:738827/1‑300 (MQ=255)                                                                                                                                  cATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGAc                                                                                                                                                                   >  1:738507/1‑300 (MQ=255)                                                                                                                                              gcgcAAAATTATTGATGTACTCTTTGAACGACGCACCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACg                                                                                                                                                       <  2:352629/300‑1 (MQ=255)                                                                                                                                                gcAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGTGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACggg                                                                                                                                                     <  1:277068/300‑1 (MQ=255)                                                                                                                                                gcAAAATTATTGATGTACTCTTGTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCGACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACggg                                                                                                                                                     <  1:640701/300‑1 (MQ=255)                                                                                                                                                       tattGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAgg                                                                                                                                              <  1:470629/300‑1 (MQ=255)                                                                                                                                                                aCTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGc                                                                                                                                     <  1:654420/300‑1 (MQ=255)                                                                                                                                                                     tttAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCa                                                                                                                                >  2:13975/1‑300 (MQ=255)                                                                                                                                                                     tttAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATCGTAGTGACGGAATGCTAACGGGTTTGGGGATTTTGAGCATTCa                                                                                                                                >  2:13961/1‑300 (MQ=255)                                                                                                                                                                           gacgCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCTACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                                                                           <  2:496543/299‑1 (MQ=255)                                                                                                                                                                           gacgCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCATGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                                                                           <  1:353704/299‑1 (MQ=255)                                                                                                                                                                           gacgCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCTGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCATTCATaacg                                                                                                                           <  1:417798/299‑1 (MQ=255)                                                                                                                                                                           gacgCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                                                                           <  2:471063/299‑1 (MQ=255)                                                                                                                                                                           gacgCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                                                                           <  1:353587/299‑1 (MQ=255)                                                                                                                                                                                 cccTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCACCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACAACAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGa                                                                                                                    >  2:292718/1‑300 (MQ=255)                                                                                                                                                                                       ggggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGc                                                                                                              <  1:303690/300‑1 (MQ=255)                                                                                                                                                                                         ggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCt                                                                                                             >  1:566151/1‑299 (MQ=255)                                                                                                                                                                                         ggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTAGCCAACGCCAGTGCCTAACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTAATAACGAACGCGATCGAGCt                                                                                                             >  1:567435/1‑299 (MQ=255)                                                                                                                                                                                              cATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGGAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTAGTCaa                                                                                                       >  1:112423/1‑300 (MQ=255)                                                                                                                                                                                              cATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGCGCCTTCATAACGAACGCGATCGAGCTGGTCaa                                                                                                       >  1:188097/1‑300 (MQ=255)                                                                                                                                                                                              cATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCaa                                                                                                       >  1:188112/1‑300 (MQ=255)                                                                                                                                                                                                        ttgtaaGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCTGGATTGTAGTGATGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCt                                                                                               <  2:619149/294‑1 (MQ=255)                                                                                                                                                                                                                  tAAAATGGCACATGTAACTTGTGTAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCATGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGTCGGCAAAACGCAAGTGCTCTTACAGACGCTTACCCAACACCAGTTCGTCTGGATGGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAGCGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAAc                                                                                   <  1:409806/300‑1 (MQ=255)                                                                                                                                                                                                                            caTGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACNGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCa                                                                         >  1:145506/1‑300 (MQ=255)                                                                                                                                                                                                                                      gtgGAAAAACTAAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCcg                                                               <  1:66685/300‑1 (MQ=255)                                                                                                                                                                                                                                          aaaaaCTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCggg                                                           <  1:283707/300‑1 (MQ=255)                                                                                                                                                                                                                                             aaCTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCTCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATATGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTg                                                         <  2:360332/299‑1 (MQ=255)                                                                                                                                                                                                                                                  aaaTGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgatggat                                                   <  1:42625/300‑1 (MQ=255)                                                                                                                                                                                                                                                    aTGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGgatga                                                 <  2:440401/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gcgagatgtgtataagagacaggCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGgatgat                                                >  1:17196/23‑300 (MQ=255)                                                                                                                                                                                                                                                         acctctcccctACGCTTCGCCCACACCAGCCCCCCACCCGACTGCTGCTCCGGACGCTTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGt                                              >  1:687548/11‑298 (MQ=255)                                                                                                                                                                                                                                                               gCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAatat                                      >  1:92981/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCAGCGGGTGATGGATGATGTCGTAatat                                      >  1:179916/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTAGGGATNGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAACAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAatat                                      >  2:224413/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    aCGCTTCGCCAACGCCAGTGCCTCACCAGGCTTCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGc                                 <  2:640489/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         tCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTcc                            <  2:144469/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           gCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAGGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCt                          >  1:239775/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aaCGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGTTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTc                       <  1:597285/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                cGCCAGTGCCTAACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCa                      >  2:328159/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                 gCCAGTGCCTCACCATGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCATAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGNTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAg                     <  2:534527/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGTTCAAAATAGGCTTGCATATTGAACTCCATAATCAGTTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAg                     <  2:37589/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAGt                    <  1:526660/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   ccgTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTAGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAGtt                   >  2:21931/3‑299 (MQ=255)                                                                                                                                                                                                                                                                                          tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTCCCCAACACCAGTTCGTCGGGATTGTAGTGAAGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAGTTGCTCAAtt           >  1:670166/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAGTGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAGTTGCTCAAtt           >  2:152402/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          tCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAGTTGCTCAAtt           >  1:238310/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                ggCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAGTTGCTCAATTATGTTa     <  1:695419/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  ctgctgCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAGTTGCTCAATTATGTTAtt   >  2:646990/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   tgctgcTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCTGCAAAACGCAAGTGCTCCTCCATACGCTTACCCCACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTTTGCACTCCCTTTCAGTTGCTCAATTATGTTAttc  <  2:401981/300‑2 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      TTGCCGCCCAGCTGAAGACTTCAGGATCTGGGTTCGTCGCCGCACCCGCGCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATATTGGGCACTCCCTTTCAGTTGCTCAATTATGTTATTT  >  NC_000913/3730264‑3730853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑