Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,391,119	G→A	F129F (TTC→TTT)	nuoN ←	NADH:quinone oxidoreductase subunit N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,391,119	0	G	A	100.0%	205.3 / NA	57	F129F (TTC→TTT)	nuoN	NADH:quinone oxidoreductase subunit N
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/25);  total (32/25)

GTCCACAGGTGGAACGGCACCAGAGAGAGTTTGAAGCCGAGGCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGGAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGAT  >  NC_000913/2390826‑2391408                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                  gTCCACAGGTGGAACGGCACCAGAGAGAGTTTGAAGCCGAGGCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACaga                                                                                                                                                                                                                                                                                             >  1:571301/1‑300 (MQ=255)               cGGCACCAGAGAGAGTTTGAAGCCGAGGCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGtt                                                                                                                                                                                                                                                                               >  1:684538/1‑300 (MQ=255)                 gCACCAGAGAGAGTTTGAAGCCGAGGCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGtt                                                                                                                                                                                                                                                                               >  1:212966/1‑298 (MQ=255)                 gCACCAGAGAGAGTTTGAAGCCGAGGCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAAAAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGCCGCCAGATGGtt                                                                                                                                                                                                                                                                               >  2:456043/1‑298 (MQ=255)                   aCCAGAGAGAGTTTGAAGCCGAGGCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCa                                                                                                                                                                                                                                                                           >  1:688432/1‑300 (MQ=255)                         gagagTTTGAAGCCGAGGCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGcc                                                                                                                                                                                                                                                                     <  1:115774/300‑1 (MQ=255)                                        aGGCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTTGTTGAGCATACCTTCGCCAAGTTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAAACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGccc                                                                                                                                                                                                                                                      <  2:647313/300‑1 (MQ=255)                                        aGGCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGccc                                                                                                                                                                                                                                                      <  2:647193/300‑1 (MQ=255)                                         ggCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCa                                                                                                                                                                                                                                                     >  1:56912/1‑300 (MQ=255)                                                        ggCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAaca                                                                                                                                                                                                                                      >  1:177342/1‑300 (MQ=255)                                                        ggCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGATTTCTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAAACGACCAGGCCAAACAGTGGCAAAGAGATCAGTTCGATACAGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAACAGGATCCCGCCAAGCGTGGCAATTAaca                                                                                                                                                                                                                                      >  2:218240/1‑300 (MQ=255)                                                                      aGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAAc                                                                                                                                                                                                                        <  2:713132/300‑1 (MQ=255)                                                                      aGCAACAGAGGCTCGTTGAGCATACCGGCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCATGCCAAACAGCGGCAAAGAGATCAGTTCGAGACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAAc                                                                                                                                                                                                                        <  2:32512/300‑1 (MQ=255)                                                                                  tCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCttgttg                                                                                                                                                                                                            <  2:190884/300‑1 (MQ=255)                                                                                  tCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAAACGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAAAGACGCAAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAAAAGGTAGAACTCATCCttgtt                                                                                                                                                                                                             >  2:658535/1‑299 (MQ=255)                                                                                    gTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTCCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACACCAGATGGTTGGCATTCGCCAGTAGGATCCCGACCAGAGCGGGAATTAACGATAACAGGTAGGACTCATcc                                                                                                                                                                                                                  >  2:689320/1‑292 (MQ=255)                                                                                       gAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCCGTGAACGTTTCTGGCGGAAAGCGTAACCGAACCGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGACANATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGtt                                                                                                                                                                                                       >  1:548962/1‑300 (MQ=255)                                                                                       gAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGtt                                                                                                                                                                                                       >  1:736841/1‑300 (MQ=255)                                                                                         gCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTtat                                                                                                                                                                                                     >  1:706385/1‑300 (MQ=255)                                                                                           aTACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTtata                                                                                                                                                                                                    <  1:721596/299‑1 (MQ=255)                                                                                             aCCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTCAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGcc                                                                                                                                                                                                 <  2:603432/300‑1 (MQ=255)                                                                                                   gCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAg                                                                                                                                                                                           <  1:641221/300‑1 (MQ=255)                                                                                                      aaGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGcc                                                                                                                                                                                         <  2:688432/299‑1 (MQ=255)                                                                                                          tttttACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCAc                                                                                                                                                                                       >  1:246841/1‑297 (MQ=255)                                                                                                                            aaCGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTg                                                                                                                                                                  <  2:432217/300‑1 (MQ=255)                                                                                                                                     tCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTc                                                                                                                                                         >  1:660085/1‑300 (MQ=255)                                                                                                                                     tCGCCAGACTGCGCATACACCAGCGCCATACCAAACAACAGGAAAGAAGACGCTGCGGCAGAAAGGATGGCATATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAAAAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCAGCCCAGCGCGGCAATTAACAACAACAGGTAAAACCAATCCTTGTTGTCGTTATAGCCTTAAAGCCACGGGTAGGCGAAAGTGCCGGTGGCGAGGCTc                                                                                                                                                         >  2:19932/1‑300 (MQ=255)                                                                                                                                              tGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAAt                                                                                                                                                <  1:473896/300‑1 (MQ=255)                                                                                                                                                 gcATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATAcc                                                                                                                                             >  1:642091/1‑300 (MQ=255)                                                                                                                                                 gcATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATAc                                                                                                                                              <  2:639950/299‑1 (MQ=255)                                                                                                                                                 gcATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATAc                                                                                                                                              <  1:432061/299‑1 (MQ=255)                                                                                                                                                 gcATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAAACGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGACCAGCGCGGCAATTAACACCAAAAGATAGAACTCATCCTTGTTGTTATTATAGCCTTCAAGCAACGGGTAGGCGAAAGTACAGGTGGAGAGGCTCGCAAACAATa                                                                                                                                               >  2:115494/1‑298 (MQ=255)                                                                                                                                                      cacCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGccc                                                                                                                                        >  1:43412/1‑300 (MQ=255)                                                                                                                                                           gcgcCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGgtgt                                                                                                                                   >  1:603427/1‑300 (MQ=255)                                                                                                                                                                agaCCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACAGTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAATAGATCAGTTCGATGCCGAGAAACATAGACGCCAGATGGTTGGCATTCGCCAGCAGGACCCCGCCCAGCGCGGCAATTAACACCAACATGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGc                                                                                                                              <  2:207428/298‑1 (MQ=255)                                                                                                                                                                aTACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGTTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGc                                                                                                                              <  2:520147/300‑1 (MQ=255)                                                                                                                                                                aTACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGc                                                                                                                              <  1:743486/300‑1 (MQ=255)                                                                                                                                                                aTACCAAACAGCAGGAAAGAAGACGCTGCGACAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACATAGACGCCAGATGGTTTGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGc                                                                                                                              <  2:263196/300‑1 (MQ=255)                                                                                                                                                                     aaaCAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGGAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGTCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGc                                                                                                                              <  2:394539/295‑1 (MQ=255)                                                                                                                                                                               aaaGAAGACGCTGCGGCCGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCAGCCCAGCGCGGCAATTAACCCCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAACCAAAGGGAGGCGAAAGTCCAGGTGGCGAGGCTCGCAAACAATACCAACACGTGGTAAAGCATGGCGAAAcaataa                                                                                                               >  2:295123/1‑295 (MQ=255)                                                                                                                                                                                  gaagaCGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAAc                                                                                                             >  1:444927/1‑299 (MQ=255)                                                                                                                                                                                       cGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCACAGCGCGGCANTTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTCTAGCCTTCAAGCAAAGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCAAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCACCGCTCAt                                                                                                       >  2:227524/1‑300 (MQ=255)                                                                                                                                                                                         cTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCa                                                                                                     >  1:663854/1‑300 (MQ=255)                                                                                                                                                                                                          gtgtATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAg                                                                                                                               <  2:504842/257‑1 (MQ=255)                                                                                                                                                                                                          gtgtATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAg                                                                                                                               >  1:504842/1‑257 (MQ=255)                                                                                                                                                                                                           tgtATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAg                                                                                   <  1:436224/300‑1 (MQ=255)                                                                                                                                                                                                              aTTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAgcgc                                                                                >  1:653461/1‑300 (MQ=255)                                                                                                                                                                                                              aTTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCAAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAgc                                                                                  >  1:710921/1‑298 (MQ=255)                                                                                                                                                                                                                               aGTGAACGTTTCGGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACATAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGTTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCcaacaa                                                                 <  2:255236/298‑1 (MQ=255)                                                                                                                                                                                                                                               gcgcatacaccagcgccataccAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAgc                                                >  1:656228/21‑299 (MQ=255)                                                                                                                                                                                                                                                            gACCAGGCCAAACAGCGGCACAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGCTCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACAGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACCATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTACATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGACGCGTTCAGcc                                   >  2:497219/1‑299 (MQ=255)                                                                                                                                                                                                                                                                aGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATa                              >  1:232759/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     aaaCAGCGGCAAAGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCg                          <  1:665066/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                aaGAGATCAGTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGAGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGagcgtagcgt              <  1:101064/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         gTTCGATACCGAGAAANAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGNTAAGCCCAATAACCGAGANCGTAGCGTTGAGGAAAt     >  1:625553/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         gTTCGATACCGAGAAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCAAGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCAAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGTGGCGTAACGTCCATAGCGCCCGCATGGCCAACAAACCAGAGCGAAACCAGCGCAGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAAt     >  2:331819/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             gATACCGAGAAACATAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGTTAGAACTCATCCTTGTTGTCGTTATAGCCTCCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGAt  <  2:212966/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                  GTCCACAGGTGGAACGGCACCAGAGAGAGTTTGAAGCCGAGGCCAACAATCATCAGGCCGAAACCTGCCAGCAACAGCGGCTCGTTGAGCATACCGTCGCCAAGGTTTTTACCCAACGCGACAAACGACAGGTCGCCAGACTGCGCATACACCAGCGCCATACCAAACAGCAGGAAAGAAGACGCTGCGGCAGAAAGGATGGTGTATTTGATACTGGCTTCCAGTGAACGTTTCTGGCGGAAAGCGTAACCGACCAGGCCAAACAGCGGCAAAGAGATCAGTTCGATACCGAGGAACAGAGACGCCAGATGGTTGGCATTCGCCAGCAGGATCCCGCCCAGCGCGGCAATTAACACCAACAGGTAGAACTCATCCTTGTTGTCGTTATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCCCGGTGTAAAGCATGGCGAAACCATCAACGCGCATCAGCGGCGTAACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGAT  >  NC_000913/2390826‑2391408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑