Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	302,842	T→A	intergenic (‑269/‑149)	paoA ← / → yagU	aldehyde dehydrogenase, Fe‑S subunit/DUF1440 domain‑containing inner membrane protein YagU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	302,842	0	T	A	100.0%	64.5 / NA	19	intergenic (‑269/‑149)	paoA/yagU	aldehyde dehydrogenase, Fe‑S subunit/DUF1440 domain‑containing inner membrane protein YagU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (10/9);  total (10/9)

TGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTAAACGTTAAGCATAGTCGGCGACAGCAGGAGGATTATTCTTAAAACGCGAATGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAATATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCA  >  NC_000913/302567‑303077                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            tGCTCATACCAGGCCTACGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTAAACGTTAAGCATAGTCGGCGACAGCAGGAGGATTATTCTTAAAACGCGAATGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGa                                                                                                                                                                                                                     >  2:384815/1‑300 (MQ=255)  gCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTAAACGTTAAGCATAGTCGGCGACAGCAGGAGGATTATTCTTAAAACGCGAATGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGa                                                                                                                                                                                                                     >  1:443391/1‑299 (MQ=255)           aGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTAATGCTTTTTTAAACTTTAAGCATAGTCGGCGACAGCAGGAGGATTATTCTTAAAACGCGAATGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGTGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGTAGAAATGATGGAGCTTatat                                                                                                                                                                                                             <  2:111712/298‑1 (MQ=255)                    gTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTAAACGTTAAGCATAGTCGGCGACAGCAGGAGGATTATTCTTAAAACGCGAATGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCNGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGAt                                                                                                                                                                                                  >  2:594983/1‑300 (MQ=255)                      aTTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTAAACGTTAAGCATAGTCGGCGACAGCAGGAGGATTATTCTTAAAACGCGAATGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTa                                                                                                                                                                                                >  1:78034/1‑300 (MQ=255)                                                ttGCGAGAGTGCTGATGCTTTTTTAAACGTTAAGCATAGTCGGCGACAGCAGGAGGATTATTCTTAAAACGCGAATGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACaaaaaaa                                                                                                                                                                      >  2:751740/1‑297 (MQ=255)                                                                                                 cagGAGGATTATTCTTAAAACGCGAATGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAag                                                                                                                           <  2:474958/294‑1 (MQ=255)                                                                                                               ttAAAACGCAAAGGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCTGGATGTTGATGATGATTGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTGTTATGAATACTCCTGAAGAGGTGTATAACATAATgg                                                                                                         <  2:140090/298‑1 (MQ=255)                                                                                                                         aaTGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGt                                                                                             <  1:384815/300‑1 (MQ=255)                                                                                                                              aGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGata                                                                                        <  2:443391/300‑1 (MQ=255)                                                                                                                                cTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGatat                                                                                       >  2:719200/1‑299 (MQ=255)                                                                                                                                                         gTTCGCCGGGATGTTGATAATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGa                                                             <  2:137924/300‑1 (MQ=255)                                                                                                                                                                          ttatgatgGTAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGGCACTTCTAAAATAAAAATAGTGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGt                                            <  2:168377/298‑1 (MQ=255)                                                                                                                                                                           gatgatgGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGANGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTc                                           >  1:549515/1‑300 (MQ=255)                                                                                                                                                                                   gAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGATTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGCATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGca                                   >  2:389298/1‑300 (MQ=255)                                                                                                                                                                                   gAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGATTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGCATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTTTTGCTGca                                   >  2:389596/1‑300 (MQ=255)                                                                                                                                                                                                    tAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGc                  >  1:348162/1‑300 (MQ=255)                                                                                                                                                                                                         gTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGTAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCg             <  2:78034/300‑1 (MQ=255)                                                                                                                                                                                                                    aaaCCAATAGTGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAAAATAAAAATAGGTAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAATAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCGGGCGTTGTTTCCGCa  <  1:373241/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            TGCTCATACCAGGCCTCCGGTATTCTAAAGGGGAAAATAAGAGTGCGTTGCGAGAGTGCTGATGCTTTTTTAAACGTTAAGCATAGTCGGCGACAGCAGGAGGATTATTCTTAAAACGCGAATGCAGCTATGAGCCCGGCTAATAAATTCATGTTCGCCGGGATGTTGATGATGATGGGAGCTGGTTTATTGCTTTAGTTGTACGATGCAAAAACCAATAAGGAAACCTGTGATTTTCAGCTCTACATCACCCTGCAAATCTCTGTCACTTCTAATATAAAAATAGGGAGAAATGATGGAGCTTATATTCATTGGCGATTAGGAAACTATCTTGTTATACAAAACAATACAGTTCTTTACATTTGCCTTGTTTTATGAATACTCCTGAAGAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCA  >  NC_000913/302567‑303077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑