Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	448,201	G→A	L154F (CTT→TTT)	cyoC ←	cytochrome bo3 subunit 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	448,201	0	G	A	100.0%	207.4 / NA	57	L154F (CTT→TTT)	cyoC	cytochrome bo3 subunit 3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/24);  total (33/24)

ATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAGACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCG  >  NC_000913/447910‑448488                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                aTTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAg                                                                                                                                                                                                                                                                                         <  1:76576/300‑1 (MQ=255)           gagaGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGTAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAg                                                                                                                                                                                                                                                                               <  1:477418/300‑1 (MQ=255)             gagGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGAc                                                                                                                                                                                                                                                                             <  2:688135/300‑1 (MQ=255)             gagGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTNTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGAc                                                                                                                                                                                                                                                                             <  1:567868/300‑1 (MQ=255)              agGCAGCTCCTGTCATCAACATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGAcc                                                                                                                                                                                                                                                                            <  1:764811/300‑1 (MQ=255)                      ccTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCgtgcgtgc                                                                                                                                                                                                                                                                    >  1:147160/1‑300 (MQ=255)                                  aTCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGANCACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGCCCAGCGCaa                                                                                                                                                                                                                                                        >  2:574595/1‑300 (MQ=255)                                      aGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGa                                                                                                                                                                                                                                                     <  2:54093/299‑1 (MQ=255)                                              aTCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACa                                                                                                                                                                                                                                            >  1:257479/1‑300 (MQ=255)                                                    gTCATAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGAAAGGAAGc                                                                                                                                                                                                                                      >  1:239324/1‑300 (MQ=255)                                                         aaTGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTg                                                                                                                                                                                                                                 <  1:134332/300‑1 (MQ=255)                                                            gATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGa                                                                                                                                                                                                                              <  2:768087/300‑1 (MQ=255)                                                              tCGACGGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAAGAAGTGCCAGAACATGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCATCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATc                                                                                                                                                                                                                            <  2:285487/300‑1 (MQ=255)                                                                    ggATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAATTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGAcc                                                                                                                                                                                                                      <  1:442065/300‑1 (MQ=255)                                                                    ggATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGAcc                                                                                                                                                                                                                      <  1:440918/300‑1 (MQ=255)                                                                                   tGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATAAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAAt                                                                                                                                                                                                       <  2:719963/300‑1 (MQ=255)                                                                                                   gCCATGGGACGCGCCGNTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCNGGACCCATGCCGTTAACANTCAGGTGATGGAATTCa                                                                                                                                                                                       >  1:666124/1‑300 (MQ=255)                                                                                                   gCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGNAGACCGTGCGTGCCGACCAGCGCAAAGAACGATGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCa                                                                                                                                                                                       >  2:633546/1‑300 (MQ=255)                                                                                                   gCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTNCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCa                                                                                                                                                                                       >  1:178252/1‑300 (MQ=255)                                                                                                   gCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCa                                                                                                                                                                                       >  1:376705/1‑300 (MQ=255)                                                                                                   gCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCa                                                                                                                                                                                       >  2:689255/1‑300 (MQ=255)                                                                                                   gCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGATGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCa                                                                                                                                                                                       >  1:376589/1‑300 (MQ=255)                                                                                                   gCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAAACAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCa                                                                                                                                                                                       >  2:689435/1‑300 (MQ=255)                                                                                                       tGGGACGCGCCGCTGTGATCGGGAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGa                                                                                                                                                                                   <  2:81404/300‑1 (MQ=255)                                                                                                                          cGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATcc                                                                                                                                                                >  1:390178/1‑300 (MQ=255)                                                                                                                                    aCTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAaac                                                                                                                                                      >  1:796260/1‑300 (MQ=255)                                                                                                                                           aCATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGANCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCa                                                                                                                                                 >  1:166652/1‑298 (MQ=255)                                                                                                                                             aTCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTc                                                                                                                                             <  2:801157/300‑1 (MQ=255)                                                                                                                                             aTCGCCCCCAGTAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGTCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAATTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTc                                                                                                                                             <  2:226778/300‑1 (MQ=255)                                                                                                                                                          gATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCAGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGCTAAATCCGGCACCAAACAAACAGGTCAAAgcca                                                                                                                                      >  2:114394/1‑294 (MQ=255)                                                                                                                                                           aTAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTTACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAAC‑AGGTCAACGCCAGCCAGgag                                                                                                                              <  1:508247/300‑1 (MQ=255)                                                                                                                                                            tAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAATTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGTTGATGGAATTCATAGATTTCCGTCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGgag                                                                                                                              <  1:119721/300‑1 (MQ=255)                                                                                                                                                             aaaCAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGTGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCNACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCACGATAAATCAGGCACAAAAAAACCAGGTCAACGCCAGCCAGgaga                                                                                                                             >  2:245735/1‑300 (MQ=255)                                                                                                                                                                     gTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCt                                                                                                                       >  1:482424/1‑298 (MQ=255)                                                                                                                                                                     gTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCAGATAAATCCGGCACCAAACAACAAGGTCAACGCCAGCCAGGAGATAACCt                                                                                                                       >  2:769439/1‑298 (MQ=255)                                                                                                                                                                                             tCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTAAGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGCCCCATGCCGTTAACCATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAACCAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACCTGGc                                                                                              >  1:687926/1‑299 (MQ=255)                                                                                                                                                                                                 ggAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGAAGGGCGATTTGTACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGGGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGAt                                                                                           >  2:24564/1‑298 (MQ=255)                                                                                                                                                                                                     gTGCCAGAACAGGCTCAGGCACATGGTGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGCGACGTGCAGACCGTGCGTGCCGATCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGTAGATAACCCGGCTTTTGTTGTTTTTGTACATGGCGATAgccgc                                                                                     <  2:110982/300‑1 (MQ=255)                                                                                                                                                                                                           gAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGcc                                                                               >  1:46249/1‑300 (MQ=255)                                                                                                                                                                                                           gAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAAACAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAAACAGGTCAAAGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGca                                                                               >  2:716147/1‑299 (MQ=255)                                                                                                                                                                                                            aaCAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAATTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCg                                                                              <  1:620943/300‑1 (MQ=255)                                                                                                                                                                                                                 gCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGt                                                                         >  1:752146/1‑300 (MQ=255)                                                                                                                                                                                                                         acaTGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGTAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGc                                                                 <  2:748208/300‑1 (MQ=255)                                                                                                                                                                                                                         acaTGAGGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGTTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGTTGATGGAGc                                                                 <  2:385040/300‑1 (MQ=255)                                                                                                                                                                                                                         acaTGAGGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGTTGATGGAGc                                                                 <  2:219481/300‑1 (MQ=255)                                                                                                                                                                                                                                   ggTACGGTTAGTGCTGGTCAGGCCGCGACGTGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCANCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAacaaca                                                        >  1:591577/1‑299 (MQ=255)                                                                                                                                                                                                                                        ggTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAAACTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGTTGAACAACAGCaa                                                    >  2:162450/1‑298 (MQ=255)                                                                                                                                                                                                                                        ggTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGAAAAGAACGCTGACAGAAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAAAAAACAGGTCAACGCCAGCCAGGAGATAACATGACTTTTGTTGTTTTTGTACCTGGCGATCGCCGCCATGCCGCAGGTGATGGCGCTTAACAACAGCAAGa                                                  >  2:272406/1‑300 (MQ=255)                                                                                                                                                                                                                                             gTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCATCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACTCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTCCATGGCGATAGCCGCCATGCCGTCGGTGATGGAGCTGAACAACAGCAAGAAAGtt                                             >  1:44463/1‑300 (MQ=255)                                                                                                                                                                                                                                             gTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCATCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACTCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGtt                                             >  1:44184/1‑300 (MQ=255)                                                                                                                                                                                                                                                  ggTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAAc                                        >  1:145567/1‑300 (MQ=255)                                                                                                                                                                                                                                                  ggTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCAGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAAc                                        >  1:146186/1‑300 (MQ=255)                                                                                                                                                                                                                                                       ggCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGCTGGAGCTGACCAACAGCAAGAAAGTTTCAACCAgaa                                   >  1:279026/1‑300 (MQ=255)                                                                                                                                                                                                                                                       ggCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCATAGGTGATGGAGCTGAACAACAGCAAGAAAATTTCCAACAgaa                                   >  1:278137/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gggCGATTTGCACCATCAGCACCGCCATCCAGATAAAACCATAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCATGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGc                           <  2:204334/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         gCACCATCAGCACCGCCATCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAAACAGGTCAACGTCAGCCAGGGGATAACATGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGACATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAATTTCAACCAgaac                                  >  2:312496/1‑283 (MQ=255)                                                                                                                                                                                                                                                                                          aTCCAGATAAAACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGTAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGTAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCg  <  2:175811/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                ATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCATGTAGGTTTTTACGCTGCCATGGGACGCGCCGCTGTGATCGGTAGAATGACTCATTACATCGCCCCCATCAGATAAACAACAGTGAACACACAGATCCAAACCACATCCAGGAAGTGCCAGAACAGGCTCAGGCACATGATGCGGGTACGGTTAGTGCTGGTCAGGCCGCGACGGGCGATTTGCACCATCAGCACCGCCATCCAGATAAGACCAGAAGTGACGTGCAGACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCG  >  NC_000913/447910‑448488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑